小麦新型化学杂交剂的筛选

doi:10.3724/SP.J.1006.2014.01125

摘要/Abstract

摘要：

为拓展小麦化控两系途径的药剂种类，降低化学杂交剂(CHA)成本，利用西农1376检验15种化学药剂、4个浓度、3个施用时期(小麦不同发育时期)的诱导不育效果, 并对花药败育的机制进行了研究。结果表明，各处理诱导小麦花粉败育效果差异较大，Feek’s 7.5和9.5时期，15种药剂各浓度处理诱导植株营养生长发育异常或花粉败育不明显；Feek’s 8.5时期，T6药剂0.24 kg hm^-2诱导雄性不育率为93.33%，植株表型及雌蕊发育正常，对其饱和授粉，能获得杂交种子，且饱和授粉结实率较高。石蜡切片观察该处理诱导雄性不育花药的绒毡层细胞降解过程异常，自单核早期绒毡层细胞核明显降解，单核中后期花粉内核也开始降解，直至三核期，绒毡层细胞及花粉内核及营养物质基本消失，仅剩少量花粉壁残留，最终导致花粉败育。因此认为，T6诱导的小麦生理型雄性不育与绒毡层的异常降解直接相关。

关键词: 小麦, 化学杂交剂, 饱和授粉, 不育率, 绒毡层

Abstract:

To expand varieties of chemical hybridization agent and reduce their cost, We selected 15 varieties of chemical hybridization agent for treating wheat cultivar Xi’nong 1376 at three growth stages with four concentrations to survey the infertile effect, phytotoxicity and pollen abortion mechanism. The results showed that there were significant differences among the chemical hybridization agent treatments. At stages Feek’s 7.5 and Feek’s 9.5, abnormal vegetative growth or pollens abortion were not obvious. While at the stage of Feek’s 8.5, the rate of male sterility of Xinong 1376 was 93.33% in 0.24 kg ha^-1 T6 treatment, and the pistil and the plant showed normal development, we also achieved a high seed setting rate via crossing the male sterile plants with normal fertile lines by saturate pollination. Paraffin sections indicated that the degradable process of tapetum cells in the male sterile anther treated by T6-C was abnormal. The nucleus of tapetum cells degraded at uninucleate early stage, and then pollen nucleus degraded at uninucleate late stage. Tapetum cells, nucleus of pollen and nutrients were almost disappeared till trinucleate stage. Finally, a little residue of pollen wall left and pollen aborted. Therefore, we hypothesize that the male sterility induced by T6 could be related to the degradation of tapetum cells in anther.

Key words: Wheat, Chemical hybrid agents, Saturate pollination, Sterility ratio, Tapetum

宋瑜龙，张鹏飞，张改生*，赵卓军，牛娜，赵新亮，王军卫. 小麦新型化学杂交剂的筛选[J]. 作物学报, 2014, 40(06): 1125-1131.

SONG Yu-Long,ZHANG Peng-Fei,ZHANG Gai-Sheng*,ZHAO Zhuo-Jun,NIU Na,ZHAO Xin-Liang,WANG Jun-Wei . Screening of a New Chemical Hybridizing Agent in Wheat[J]. Acta Agron Sin, 2014, 40(06): 1125-1131.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2014.01125

https://zwxb.chinacrops.org/CN/Y2014/V40/I06/1125

参考文献

[1]金双义, 范军. 对小麦杂种优势研究方法的探讨. 中国新技术新产品, 2008, 12(上): 156

Jin S Y, Fan J. Study on heterosis of research methods in wheat. China New Technol Prod, 2008, 12(scroller): 156 (in Chinese)

[2]辛金霞, 戎郁萍. 化学杂交剂在植物育种中的应用现状. 草业科学, 2010, 27(3): 124–131

Xin J X, Rong Y P. Application status of chemical hybridizing agent in plant breeding. Pratacul Tural Sci, 2010, 27(3): 124–131 (in Chinese)

[3]魏正平, 刘树人, 翟玉洁, 王岩, 于天峰, 刁艳玲. 化学杂交剂BAU-2诱导春小麦雄性不育的研究. 北京农业大学学报, 1993, 19(增刊): 25–29

Wei Z P, Liu S R, Zhai Y J, Wang Y, Yu T F, Diao Y L. Studies on the male sterility induced by CHA-BAU-2 in spring wheat. Acta Agric Univ Pekinensis, 1993, 19(suppl): 25–29 (in Chinese with English abstract)

[4]黄铁城, 王明理, 张爱民, 张仲琦. 新型化学杂交剂WL84811诱导普通小麦(Triticum aestivum)雄性不育的研究. 作物学报, 1988, 14(2): 155–162

Huang T C, Wang M L, Zhang A M, Zhang Z Q. Studies on the male sterility in wheat (Triticum aestivum) induced by new gametocide WL84811. Acta Agron Sin, 1988, 14(2): 155–162 (in Chinese with English abstract)

[5]梁肖青. SC2053诱导小麦雄性不育的效果研究. 山西农业大学学报, 2006, 5(5): 92–94

Liang X Q. Analysis on the male sterility in wheat induced by SC2053. J Shanxi Agric Univ, 2006, 5(5): 92–94 (in Chinese with English abstract)

[6]高庆荣, 刘保申, 于金凤, 孙兰珍. 化学杂交剂GENESIS对小麦的杀雄效果. 山东农业大学学报(自然科学版), 2002, 33(1): 1–6

Gao Q R, Liu B S, Yu J F, Sun L Z. The Effect of CHA-GENESISI on growth and development of winter wheat. J Shandong Agric Univ (Nat Sci Edn), 2002, 33(1): 1–6 (in Chinese with English abstract)

[7]王振华, 刘宏伟, 张改生, 王军卫, 马守才. 3种新型化学杂交剂诱导小麦雄性不育效果比较. 西北农林科技大学(自然科学版), 2003, 31(3): 43–46

Wang Z H, Liu H W, Zhang G S, Wang J W, Ma S C. Effects on male sterility induced by three kinds of chemical hybridization agent in wheat. J Northwest Sci Tech Univ Agric For (Nat Sci Edn), 2003, 31(3): 43–46 (in Chinese with English abstract)

[8]武计萍, 胡金锁, 李国强, 仇松英, 逯腊虎, 孟兆萍. 化学杂交剂SQ-1诱导小麦雄性不育及与不同小麦品种互作效应的研究. 小麦研究, 2006, 27(3): 12–15

Wu J P, Hu J S, Li G Q, Qiu S Y, Lu L H, Meng Z P. Effect of male sterility on different wheat genotype induced by SQ-1. Wheat Res, 2006, 27(3): 12–15 (in Chinese with English abstract)

[9]叶景秀, 陈蕊红, 张改生, 王俊生, 王书平, 李莉, 牛娜, 马守才, 李红霞, 朱建楚.杀雄剂CULT诱导小麦雄性不育花药蛋白质组分分析. 农业生物技术学报, 2009, 17: 858–864

Ye J X, Chen R H, Zhang G S, Wang J S, Wang S P, Li L, Niu N, Ma S C, Li H X, Zhu J C. Analysis on anther proteins of wheat male sterile line induced by chemical hybridizing agent SQ-1. J Agric Biotechnol, 2009, 17: 858?864 (in Chinese with English abstract)

[10]宋瑜龙, 王亮明, 张改生, 盛英, 李亚鑫, 牛娜, 赵卓军. 杀雄剂SQ-1诱导谷子雄性不育研究. 作物学报, 2011, 37: 1695–1700

Song Y L, Wang L M, Zhang G S, Sheng Y, Li Y X, Niu N, Zhao Z J. Male sterility induced by chemical hybridizing agent SQ-1 in Setaria italica Beauv. Acta Agron Sin, 2011, 37: 1695–1700 (in Chinese with English abstract)

[11]盛英, 张改生, 李亚鑫, 张龙雨, 王书平, 赵新亮, 王亮明, 宋瑜龙. 小麦生理型雄性不育花药绒毡层和孢粉素变化与 RAFTIN1表达的关系. 中国农业科学, 2011, 44: 3937–3944

Sheng Y, Zhang G S, Li Y X, Zhang L Y, Wang S P, Zhao X L, Wang L M, Song Y L. The relationship on anther tapetum, sporopollenin and expression of RAFTIN1 in physiological male sterile wheat. Sci Agric Sin, 2011, 44: 3937–3944 (in Chinese with English abstract)

[12]尹梦回, 董静, 李先碧, 侯磊, 罗明, 李德谋, 裴炎, 肖月华. 烟草绒毡层特异启动子pTA29在棉花中的表达特性. 作物学报, 2008, 34(12): 2092–2098

Yin M H, Dong J, Li X B, Hou L, Luo M, Li D M, Pei Y, Xiao Y H. Expression characteristics of tobacco tapetum-specific promoter pTA29 in cotton. Acta Agron Sin, 2008, 34: 2092–2098 (in Chinese with English abstract)

[13]Li Y Y, Yang Q, Yan J Y, Chen M. Cytoplasmic male-sterility of onion (Allium cepa L.) 63A is associated with premature degeneration of the tapetum. Acta Agron Sin, 2006, 32(03): 369–372

[14]苏少泉. 草甘膦述评. 农药, 2005, 44(4): 145–149

Su S Q. Glyphosate review. Chin J Pesticides, 2005, 44(4): 145–149 (in Chinese with English abstract)

[15]许海霞, 吕德彬, 程西永, 詹克慧, 陈军营, 姜鸿勋. GENESIS诱导小麦雄性不育的形态学和细胞学观察. 河南农业大学学报, 2003, 9: 205–208

Xu H X, Lü D B, Cheng X Y, Zhan K H, Chen J Y, Jiang H X. Morphological and cytological observations of male sterility induced by CHA GENESIS in wheat. J Henan Agric Univ, 2003, 9: 205–208 (in Chinese with English abstract)

[16]徐如强, 黄铁城. “BAU-2”诱导普通小麦雄性不育的研究: III. 化学杀雄机制. 北京农业大学学报, 1993, 19(增刊): 19–24

Xu R Q, Huang T C. Studies on BAU-2 induced male sterility in winter wheat (T. aestivum): III. The methanism of BAU-2 inducing male sterility. Acta Agric Univ Pekinesis, 1993, 19(suppl): 19–24 (in Chinese with English abstract)

[17]范平, 崔党群, 范弘伟. CHA-SC2053诱导普通小麦雄性不育研究. 河南农业大学学报, 1998, 32: 149–153

Fan P, Cui D Q, Fan H W. Studies on the male sterility induced by CHA-SC2053 in common wheat. Acta Agric Univ Henanensis, 1998, 32: 149–153 (in Chinese with English abstract)

[18]杨武云, 饶世达. 小麦新型化杀剂SC2053诱导花粉败育的机制研究. 北京农业大学学报, 1993, 19(增刊): 88–91

Yang W Y, Rao S D. Cytological study on the new type chemical hybridizing agent SC2053 inducing wheat pollen abortion. Acta Agric Univ Pekinesis, 1993, 19(suppl): 88–91 (in Chinese with English abstract)

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