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作物学报 ›› 2015, Vol. 41 ›› Issue (04): 601-612.doi: 10.3724/SP.J.1006.2015.00601

• 耕作栽培·生理生化 • 上一篇    下一篇

玉米叶片气孔特征及气体交换过程对气候变暖的响应

郑云普1,2,4,徐明2,*,王建书3,邱帅2,王贺新4   

  1. 1河北工程大学水电学院,河北邯郸 056038;2中国科学院地理科学与资源研究所生态系统网络观测与模拟重点实验室,北京 100101;
    3河北工程大学农学院,河北邯郸 056038;4大连大学现代农业研究院,辽宁大连 116622
  • 收稿日期:2014-11-03 修回日期:2015-02-06 出版日期:2015-04-12 网络出版日期:2015-03-03
  • 通讯作者: 徐明,E-mail: mingxu@igsnrr.ac.cn
  • 基金资助:

    本研究由国家重点基础研究发展计划项目(2012CB417103),国家自然科学基金青年项目(31400418),国家公益性行业(农业)科研专项(201303022)和中国博士后科学基金面上项目(2014M561044)资助。

Responses of the Stomatal Traits and Gas Exchange of Maize Leaves to Climate Warming

ZHENG Yun-Pu1,2,4,XU Ming2,*,WANG Jian-Shu3,QIU Shuai2,WANG He-Xin3   

  1. 1 School of water conservancy and hydropower, Hebei University of Engineering, Handan 056038, China; 2 Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographical Sciences and Natural Resources, Chinese Academy of Sciences, Beijing 100101, China; 3School of agriculture, Hebei University of Engineering, Handan 056038, China; 4Institute of Modern Agricultural Research, Dalian University, Dalian 116622, China?
  • Received:2014-11-03 Revised:2015-02-06 Published:2015-04-12 Published online:2015-03-03
  • Contact: 徐明,E-mail: mingxu@igsnrr.ac.cn

摘要:

气孔是植物叶片表面控制大气与植物间气体交换的孔状结构, 对于生态系统碳、水循环过程的调节起着非常重要的作用。本文利用典型农田生态系统实验增温平台,研究了未来气候变暖对玉米叶片的气孔特征(包括气孔频度、气孔开口大小和形状以及气孔分布格局)和气体交换过程的影响。结果表明:(1)尽管增温并没有改变气孔密度(P>0.05),但却由于表皮细胞数目的减少导致气孔指数显著增加12% (P<0.05);(2)增温使气孔开口的长度显著减小18% (P<0.01),宽度增加26% (P<0.01),面积和周长分别增加31% (P <0.01)和13% (P<0.05);(3)实验增温还使单个气孔之间最近邻域的平均距离显著增加,表明气孔在玉米叶片上的分布变得更加均匀;(4)增温导致玉米叶片的净光合反应速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)分别增加52% (P<0.05)、163% (P<0.001)和81% (P<0.05);与此相反,玉米叶片的暗呼吸速率(Rd)却显著降低24% (P<0.01)。增温没有对细胞间CO2浓度(Ci)和水分利用效率(WUE)产生显著的影响(P>0.05)。本研究结果表明,未来全球气候变暖可能通过改变玉米叶片的气孔频度、气孔开口大小和形状及其在叶片上的空间分布格局来改变其气体交换过程。

关键词: 全球变暖, 玉米, 气孔特征, 气体交换, 华北平原

Abstract:

Stomata are the pores on leaf surfaces controlling gas exchanges, mainly CO2 and water vapor, between the atmosphere and plants, and thus regulate carbon and water cycles in various ecosystems. This study investigated the effects of experimental warming on the stomatal frequency, stomatal aperture size and shape, and stomatal distribution pattern, and their relationships with the leaf gas exchange rates of maize (Zea may L.) leaves through a field manipulative warming experiment with infrared heaters in a typical agriculture ecosystem in the North China Plain. Our results showed that experimental warming had little effect on stomatal density, but increased stomatal index by 12% (P<0.05) due to the reduction in the number of epidermal cells under the warming treatment. Warming also decreased stomatal aperture length by 18% (P<0.01) and increased stomatal aperture width 26% (P<0.01). As a result, experimental warming increased the average stomatal aperture area by 31% (P<0.01) and stomatal aperture circumference by 13% (P<0.05), and resulted in a more regular stomatal distribution on both the adaxial and abaxial surfaces in leaves with an increased average nearest neighbor distance between stomata. In addition, experimental warming also affacted the gas exchange of maize leaves. Experimental warming significantly increased net photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr) by 52% (P<0.05), 163% (P<0.001), and 81% (P<0.05), respectively. Meanwhile, experimental warming decreased the leaf dark respiration(Rd) by 24% (P<0.01), but had no significant effects on intercellular CO2 concentration (Ci) and water use efficiency (WUE; P>0.05). In conclusion, the experimental warming may affect the gas exchange of maize leaves through the changes of the stomatal traits including stomatal frequency, stomatal aperture size and shape, and stomatal distribution on leaves.

Key words: Global warming, Maize, Stomatal traits, Gas exchange, The North China Plain

[1]Woodward F I. Stomatal numbers are sensitive to increases in CO2 from preindustrial levels. Nature, 1987, 327: 617–618



[2]Hetherington A M, Woodward F I. The role of stomata in sensing and driving environmental change. Nature, 2003, 424: 901–908



[3]Franks P J, Beerling D J. Maximum leaf conductance driven by CO2 effects on stomatal size and density over geologic time. Proc Natl Acad Sci USA, 2009, 106: 10343–10347



[4]Haworth M, Heath J, McElwain J C. Differences in the response sensitivity of stomatal index to atmospheric CO2 among four genera of Cupressaceae conifers. Ann Bot, 2010, 105: 411–418



[5]Taylor S H, Franks P J, Hulme S P. Photosynthetic pathway and ecological adaptation explain stomatal trait diversity amongst grasses. New Phytol, 2012, 193: 387–396



[6]Lake J A, Woodward F I. Response of stomatal numbers to CO2 and humidity: control by transpiration rate and abscisic acid. New Phytol, 2008, 179: 397–404



[7]Ciais P, Denning A S, Tans P P. A three-dimensional synthesis of vegetation feedbacks in doubled CO2 climate experiments. J Geophys Res, 1997, 102: 5857–5872



[8]Buckley T N, Farquhar G D, Mott K A. Qualitative effects of patchy stomatal conductance distribution features on gas-exchange calculations. Plant Cell Environ, 1997, 20: 867–880



[9]Apple M E, Olszyk D M, Ormrod D P. Morphology and stomatal function of douglas fir needles exposed to climate change: Elevated CO2 and temperature. Int J Plant Sci, 2000, 161: 127–132



[10]Hovenden M J. The influence of temperature and genotype on the growth and stomatal morphology of southern beech, Nothofagus cunninghamii (Nothofagaceae). Aust J Bot, 2001, 49: 427-434



[11]Kouwenberg L L R, Kürschner W M, McElwain J C. Stomatal frequency change over altitudinal gradients: Prospects for paleoaltimetry. Rev Mineral Geochem, 2007, 66: 215–241



[12]Fraser L H, Greenall A, Carlyle C. Adaptive phenotypic plasticity of Pseudoroegneria spicata: response of stomatal density, leaf area and biomass to changes in water supply and increased temperature. Ann Bot, 2009, 103: 769–775



[13]Beerling D J, Chaloner W G. The impact of atmospheric CO2 and temperature change on stomatal density: Observations from Quercus robur Lammas leaves. Ann Bot, 1993, 71: 231–235



[14]Ferris R, Nijs I, Behaeghe T. Elevated CO2 and temperature have different effects on leaf anatomy of perennial ryegrass in spring and summer. Ann Bot, 1996, 78: 489–497



[15]Reddy K R, Robana R R, Hodges H F. Interactions of CO2 enrichment and temperature on cotton growth and leaf characteristics. Environ Exp Bot, 1998, 39: 117–129



[16]Xu Z Z, Zhou G S, Shimizu H. Effects of soil drought with nocturnal warming on leaf stomatal traits and mesophyll cell ultrastructure of a perennial grass. Crop Sci, 2009, 49: 1843–1851



[17]Xu Z Z, Zhou G S. Effects of water stress and high nocturnal temperature on photosynthesis and nitrogen level of a perennial grass Leymus chinensis. Plant Soil, 2005, 269: 131–139



[18]张立荣, 牛海山, 汪诗平, 李英年, 赵新全. 增温与放牧对矮嵩草草甸4种植物气孔密度和气孔长度的影响. 生态学报, 2010, 30: 6961–6969



Zhan  L R, Niu H S, Wang S P, Li Y N, Zhao X Q. Effects of temperature increase and grazing on stomatal density and length of four alpine Kobresia meadow species, Qinghai-Tibetan Plateau. Acta Ecol Sin, 2010, 30: 6961–6969 (in Chinese with English abstract)



[19]Croxdale J L. Stomatal patterning in monocotyledons: Tradescantia as a model system. J Exp Bot, 1998, 49: 279–292



[20]Berger D, Altmann T. A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis thaliana. Genes Dev, 2000, 14: 1119–1131



[21]Croxdale J L. Stomatal patterning in angiosperms. Am J Bot, 2000, 87: 1069–1080



[22]Bergermann D C, Lukowitz W, Somerville C R. Stomatal development and pattern controlled by a MAPKK Kinase. Science, 2004, 304: 1494–1497



[23]Juarez M, Twigg R, Timmermans M. Specification of adaxial cell fate during maize leaf development. Development, 2004, 131: 4533-4544



[24]Wang H, Ngwenyama N, Liu Y. Stomatal development and patterning are regulated by environmentally responsive mitogen-actived protein kinases in Arabidopsis. Plant Cell, 2007, 19: 63–73



[25]Casson S A, Hetherington A M. Environmental regulation of stomatal development. Curr Opin Plant Biol, 2010, 13: 90–95



[26]Ciha A J, Brun W A. Stomatal size and frequency in soybeans. Crop Sci, 1975, 15: 309–313



[27]Green R L, Beard J B, Casnoff D M. Leaf blade stomatal characterizations and evapotranspiration rates of 12 cool-season perennial grasses. HortScience, 1990, 25: 760–761



[28]Driscoll S P, Prins A, Olmos E. Specification of adaxial and abaxial stomata, epidermal structure and photosynthesis to CO2 enrichment in maize leaves. J Exp Bot, 2006, 57: 381–390



[29]Salisbury E J. On the causes and ecological significance of stomatal frequency, with special reference to the woodland flora. Philos Trans R Soc Lond B Biol Sci, 1927, 216: 1–65



[30]Sharma G K, Dunn D B. Environmental modifications of leaf surface traits in Datura stramonium. Can J Bot, 1969, 47: 1211–1216



[31]Tichá I. Photosynthetic characteristics during ontogenesis of leaves. 7. Stomata density and sizes. Photosynthetica, 1982, 16: 375–471



[32]Zacchini M, Morini S, Vitagliano C. Effect of photoperiod on some stomatal characteristics of in vitro cultured fruit tree shoots. Plant Cell Tissue Organ Cult, 1997, 49: 195–200



[33]Stancato G C, Mazzoni-Viveiros S C, Luchi A E. Stomatal characteristics in different habitat forms of Brazilian species of Epidendrum (Orchidaceae). Nord J Bot, 1999, 19: 271–275



[34]Tao F, Yokozawa M, Xu Y, Hayashi Y, Zhang Z. Climate changes and trends in phenology and yields of field crops in China, 1981–2000. Agric For Meteorol, 2006, 138: 82–92



[35]Lin E. Agricultural vulnerability and adaptation to global warming in China. Water Air Soil Pollut, 1996, 92: 63–73



[36]Mo X G, Liu S X, Lin Z H, Guo R P. Regional crop yield, water consumption and water use efficiency and their responses to climate change in the North China Plain. Agric Ecosyst Environ, 2009, 134: 67–78



[37]Liu S X, Mo X G, Lin Z H, Xu Y Q, Ji J J, Wen G, Richey J. Crop yield responses to climate change in the Huang-Huai-Hai Plain of China. Agric Water Manage, 2010, 97: 1195–1209



[38]Tao F L, Zhang S, Zhang Z. Spatiotemporal changes of wheat phenology in China under the effects of temperature, day length and cultivar thermal characteristics. Eur J Agron, 2012, 43: 201–212



[39]Hou R X, Ou-Yang Z, Li Y S. Is the change of winter wheat yield under warming caused by shortened reproductive period? Ecol Evol, 2012, 2: 2999–3008



[40]Lobell D B, Field C B. Global scale climate–crop yield relationships and the impacts of recent warming. Environ Res Lett, 2007, 2: 1–7



[41]Ripley B D. The second-order analysis of stationary processes. J Appl Prob, 1976, 13: 255–266



[42]Lomax B H, Woodward F I, Leitch I J. Genome size as a predictor of guard cell length in Arabidopsis thaliana is independent of environmental conditions. New Phytol, 2009, 181: 311–314



[43]Djanaguiraman M, Prasad P V V, Boyle D L. High-temperature stress and soybean leaves: leaf anatomy and photosynthesis. Crop Sci, 2011, 51: 2125–2131

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