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作物学报 ›› 2015, Vol. 41 ›› Issue (04): 666-670.doi: 10.3724/SP.J.1006.2015.00666

• 研究简报 • 上一篇    

铝离子胁迫下大豆根尖柠檬酸的分泌及SGA1基因的表达

杨列耿2,**,杨曙2,**,张永先2,唐健1,黎晓峰2,*   

  1. 1广西优良用材林资源培育重点实验室 / 广西林业科学研究院, 广西南宁530002; 2广西大学 / 亚热带生物资源保护与利用国家重点实验室，广西南宁 530005
  • 收稿日期:2014-11-02 修回日期:2015-02-06 出版日期:2015-04-12 网络出版日期:2015-03-03
  • 通讯作者: 黎晓峰, E-mail: lxf@gxu.edu.cn, Tel: 0771-3235314
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2014CB138701), 国家自然科学基金项目(30771287), 广西优良用材林资源培育重点实验室开发课题基金(12A0202)和教育部博士学科点专项科研资金(200805930008)资助。

Secretion of Citrate from Root Apices and Expression of SGA1 in Soybean under AlCl3 Stress

YANG Lie-Geng2,**,YANG Shu2,**,ZHANG Yong-Xian2,TANG Jian1,LI Xiao-Feng2,*   

  1. 1 Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation / Guangxi Forestry Research Institute, Nanning 530002, China; 2 Guangxi University / State Key Laboratory for Conservation and Utilization of Subtropical Agrobioresources, Nanning 530004, China
  • Received:2014-11-02 Revised:2015-02-06 Published:2015-04-12 Published online:2015-03-03
  • Contact: 黎晓峰, E-mail: lxf@gxu.edu.cn, Tel: 0771-3235314

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摘要：

为揭示铝离子诱导大豆根尖分泌有机酸的特点及介导有机酸分泌的信号途径，采用溶液培养试验方法调查AlCl3对大豆品种广州本地2号根尖有机酸分泌及SGA1基因表达的影响。结果表明，AlCl3胁迫下大豆活体根尖分泌柠檬酸，且分泌量随着铝浓度(25、50 µmol L–1AlCl3)和处理时间(2~12 h)的增加而增加；大豆根尖以模式II分泌柠檬酸，处理后的前4 h，分泌速率很低，其后显著提升；有机酸明显分泌的诱导期长达6 h；在50 µmol L–1 AlCl3的溶液中添加异三聚体G蛋白抑制剂百日咳毒素(200 ng mL–1)，柠檬酸分泌减少38.7%。RT-PCR分析结果显示，AlCl3溶液诱导大豆根尖SGA1基因的表达，其表达水平随着铝处理时间(0.5~12.0 h)的延长有提升的趋势，而诱导的SGA1基因表达明显早于有机酸开始分泌时间(6 h)。这些结果表明，铝离子诱导大豆根尖分泌柠檬酸及SGA1基因的表达，异三聚体G蛋白可能作为铝胁迫信号开关器参与有机酸分泌的调控。

关键词: AlCl3, 大豆, 柠檬酸, 异三聚体G蛋白, SGA1基因

Abstract:

The effects of Al3+ on the secretion of organic acids from root apices and the expression of SGAI gene were investigated by hydroponics to elucidate the characteritics of organic acid secretion and Al3+ stress signal transduction pathway which mediates the secretion of organic acids in soybean Guangzhou 2. The results showed that soybean root apices (in vivo) secreted citrate under Al3+ stress. The secretion of citrate increased with the increase of Al3+ concentrations (25, 50 µmol L–1 AlCl3) and the prolongation (2–12 hours) of treatment with Al3+. Citrate was secreted from root apices by pattern II in soybean. The secretion rate was very low within initial four hours after Al3+ treatment but remarkably elevated thereafter. A gap of time between the secretion and Al3+ treatment reached to about six hours. On the other hand when cholera toxin, an inhibitor of heterotrimeric G-protein, was added to Al3+solution, the amount of citrate secreted decreased by 38.7%. RT-PCR analysis results indicated that Al3+ induced SGA1 expression. In general, the expression level was elevated with the prolongation of treatment with Al3+ (0.5 to 12 hours). Moreover, Al3+ induced expression of SGA1 sooner than the secretion of citrate. These results imply that Al3+ induces  the secretion of citrate from root apices and SGA1 expression in the soybean, and heterotrimeric G proteins may act as a switch of Al3+ stress signal to be involved in the regulation of citrate secretion from root apices under Al3+ stress.

Key words: AlCl3, Soybean, Citrate, Heterotrimeric G-protein, SGA1 gene

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