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作物学报 ›› 2015, Vol. 41 ›› Issue (06): 956-962.doi: 10.3724/SP.J.1006.2015.00956

• 耕作栽培·生理生化 • 上一篇    下一篇

外源水杨酸对Cu胁迫下水培烟草生长及营养元素吸收利用的影响

徐根娣1,2,葛淑芳1,2,章艺3,吴玉环4,梅笑漫5,刘鹏1,2,*   

  1. 1 浙江师范大学生态学研究所, 浙江金华 321004；2 浙江师范大学植物学实验室, 浙江金华 321004；3 浙江旅游职业学院, 浙江杭州 3112313；4 杭州师范大学生命与环境科学学院, 浙江杭州 330036；5 杭州师范大学初等教育学院，浙江杭州 330036
  • 收稿日期:2014-10-27 修回日期:2015-04-02 出版日期:2015-06-12 网络出版日期:2015-04-14
  • 基金资助:

    本研究由国家自然科学基金项目(30970188, 30540056)和浙江省公益技术研究农业项目(2011C22053)。

Effect of Salicylic Acid on Growth and Nutrient Uptake in Hydroponic Tobacco under Cu Stress

XU Gen-Di1,2,GE Shu-Fang1,2,ZAHNG Yi3,WU Yu-Huang4,MEI Xiao-Man5,LIU Peng1,2,*   

  1. 1 Research Institute of Ecology, Zhejiang Normal University, Jinhua 321004, China; 2 Key Laboratory of Botany / Zhejiang Normal University, Jinhua 321004, China; 3 Tourism College of Zhejiang, Hanzhou 311231, China; 4 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; 5 College of Biological and Environmental Sciencess, Hanzhou Normal University, Hangzhou 330036, China
  • Received:2014-10-27 Revised:2015-04-02 Published:2015-06-12 Published online:2015-04-14

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摘要：

以耐Cu性较强的烟草品种W38 (Nicotiana tabacum cv. W38)和耐Cu性较弱的本氏烟(Nicotiana benthamiana)为试验材料，设置水培处理组即CK (Cu2+ 0 mg L–1，SA 0 μmol L–1，即对照组)、T1(Cu2+ 4 mg L–1，SA 0 μmol L–1)、T2 (Cu2+ 4 mg L–1，SA 100 μmol L–1)、T3 (Cu2+ 4 mg L–1，SA 300 μmol L–1)、T4 (Cu2+ 4 mg L–1，SA 500 μmol L–1)，探讨SA对烟草生长特性及根、茎、叶不同器官中元素吸收规律的影响。结果表明，经过4 mg L–1 Cu胁迫15 d后，2个品种的烟草生长均受到一定程度的抑制，不同器官中Cu含量均显著升高，K、Ca、Mg、Fe、Zn、B、Mn的吸收受到抑制，而在营养液中添加适宜浓度的SA能够有效缓解Cu胁迫对2个品种烟草根长、株高、鲜重的抑制作用，并降低烟草体内Cu含量。与T1处理组相比，本氏烟的根、茎、叶中Cu含量最大分别下降25.05%、39.78%和22.91%，W38的根、茎、叶中的Cu含量最大分别下降23.27%、37.30%和28.88%，并促进了营养元素K、Ca、Mg、Fe、Mn的吸收，但是对Zn、B元素的吸收影响并不明显。由此可知，高浓度的Cu胁迫会抑制烟草的生长及营养元素的吸收和运输，施加适宜浓度的外源水杨酸有利于营养元素的吸收和促进烟草的生长发育，在本试验浓度范围内施加300 μmol L–1 的水杨酸可显著缓解Cu胁迫对烟草的抑制作用。

关键词: SA, Cu胁迫, 烟草, 微量元素

Abstract:

A hydroponic experiment was conducted to study the effects from treatmeats including CK ( Cu2+ 0 mg L–1, SA 0 μmol L–1 as control), T1 (Cu2+ 4 mg L–1, SA 0 μmol L–1), T2 (Cu2+ 4 mg L–1, SA 100 μmol L-1), T3 (Cu2+ 4 mg L–1, SA 300 μmol L–1), T4 (Cu2+ 4 mg L–1, SA 500 μmol L–1)on growth characteristics, elements absorption and accumulation in two tobacco cultivars, N. tabacum cv. W38 (Cu resistant genotype) and N. benthamiana (Cu sensitive genotype) under copper stress. The result showed that the growth of two tobacco cultivars under 4 mg L–1 Cu stress for 15 days was inhibited to some extent, meanwhile, the content of Cu in their organs was increased while the absorptions of K, Ca, Mg, Fe, Zn, B, and Mn were inhibited. The addition of exogenous SA could alleviate the inhibition of plant height, root length and fresh weight, and reduce Cu content of tobacco, but increase the absorption of nutritional elements K, Ca, Mg, Fe, and Mn. Compared with treatment T1, contents of Cu in root, stem and leaf were increased by 25.05%, 39.78%, 22.91% in N. benthamiana, and by 23.27%, 37.30%, 28.88% in N. tabacum cv.W38, respectively. In conclusion, appropriate concentration of SA promotes the growth and elements absorption in tobacco under copper stress. In this experiment, 300 μmol L–1SA could significantly alleviate copper inhibition to tobacco.

Key words: Salicylic acid, Cu stress, Tobacco, Trace elements

[1]Poll E A, Schzendbel A. Hevey metals ignaling in plants: linking celluar and organismic responses. Cur Gen, 2003, 4: 187–202

[2]高培培, 章艺, 吴玉环, 徐根娣, 刘鹏. 外源水杨酸对铝胁迫下栝楼光合特性及耐铝性的影响. 水土保持学报, 2012, 26(6): 268–273

Gao P P, Zhang Y, Wu Y H, Xu G D, Liu P. Effect of exogenous SA on photosynthesis and Al tolerance of Trichosanthes kirilowii Maxim under aluminum stress. J Soil Water Con, 2012, 26(6): 268–273 (in Chinese with English abstract)

[3]张营, 李法云, 严霞. 外源K+和水杨酸在缓解融雪剂对油松幼苗生长抑制中的效应与机理. 生态学报, 2012, 32: 4300–4308

Zhang Y, Li F Y, Yan X. Alleviation effect and mechanism of exogenous potassium nitrate and salicylic acid on the growth inhibition of Pinus tabulaeformis seedlings induced by deicing salts. Acta Ecol Sin, 2012, 32: 4300–4308 (in Chinese with English abstract)

[4]Hayat Q, Hayat S, Irfan M. Effect of exogenous salicylic acid under changing environment. Environ Exp Bot, 2010, 68(1): 14–25

[5]郝敬虹, 易旸, 尚庆茂, 董春娟, 张志刚. 干旱胁迫下外源水杨酸对黄瓜幼苗膜脂过氧化和光合特性的影响. 应用生态学报, 2012, 23: 717–723

Hao J H, Yi Y, Shang Q M, Dong C J, Zhang Z G. Effects of exogenous salicylic acid on membrane lipid peroxidation and photosynthetic characteristics of Cucumis sativus seedlings under drought stress. Chin J Appl Ecol, 2012, 23: 717–723 (in Chinese with English abstract)

[6]易小林, 杨丙贤, 宗学凤, 李名扬. 信号分子水杨酸减缓干旱胁迫对紫御谷光合和膜脂过氧化的副效应. 生态学报, 2011, 31(1): 67–74

Yi X L, Yang B X, Zong X F, Li M Y. Signal chemical salicylic acid mitigates the negative effects of drought on photosynthesis and membrane lipid peroxidation of purple majesty. Acta Ecol Sin, 2011, 31(1): 67–74 (in Chinese with English abstract)

[7]Wang X M, Yuan X J, Han S, Zha H W, Sun X C, Huang J H, Liu Y N. Aniline modified hypercrosslinked polystyrene resins and their adsorption equilibriums, kinetics and dynamics towards salicylic acid from aqueous solutions. Chem Eng J, 2013, 233: 124–131

[8]张玉秀, 柴团耀. 植物耐金属机理研究进展. 植物学报, 1999, 41: 453–457

Zhang Y X, Chai T Y. Research advances on the mechanisms of heavy metal tolerance in plants. Acta Bot Sin, 1999, 41: 453–457 (in Chinese with English abstract)

[9]Ashraf M, Iris F, Manfred G. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol, 2003, 132: 272–281

[10]刘素纯, 萧浪涛, 廖柏寒, 匡逢春, 黄运湘. 铅、镉胁迫对黄瓜幼苗内源水杨酸的影响. 湖南农业科学, 2006, (3): 25–28

Liu S C, Xiao L T, Liao B H, Kuang F C, Huang Y X. Effects on endogenous salicylic acid of cucumber seedling sunder lead and cadmium stress. Hunan Agric Sci, 2006, (3): 25–28 (in Chinese with English abstract)

[11]Freeman J L, Garcia D, Kim D, Hopf A, Salt D E. Constitutively elevated salicylic acid signals glutathione-mediated nickel tolerance in thlaspinickelhyper accumulators. Plant Physiol, 2005, 137: 1082–1091

[12]Drazic G, Mihailovic N. Modification of cadmium toxicity in soybean seedlings by salicylic acid. Plant Sci, 2005, 168: 511–517

[13]张艳英, 周楠, 刘鹏, 徐根娣, 陈文荣, 蔡妙珍. 铜胁迫对烟草幼苗氮代谢的影响. 生态学报, 2009, 29: 6779–6784

Zhang Y Y, Zhou N, Liu P, Xu G D, Chen W R, Cai M Z. Effect of Cu stress on nitrogen metabolism of Nicotiana tabacum L. seedling. Acta Ecol Sin, 2009, 29: 6779–6784 (in Chinese with English abstract)

[14]张小梅, 王智鑫, 李广录. 水杨酸对铬胁迫下绿豆幼苗生长抑制的缓解效应. 河南农业科学, 2012, 41(11): 52–54

Zhang X M, Wang Z X, Li G L. Mitigating effects of SA on inhibition of Cr2+ stress to mung bean seedling growth. Henan Agric Sci, 2012, 41(11): 52–54 (in Chinese with English abstract)

[15]计汪栋, 施国新, 张慧, 徐勤松, 许晔, 杜开和. 菹草对Hg2+胁迫的生理和结构应答反应. 生态学报, 2007, 27: 2856–2863

Ji W D, Shi G X, Zhang H, Xu Q S, Xu Y, Du K H. Physiolgical and ultrastructural responses of Potamogeton crispus to Hg2+ stress. Acta Ecol Sin, 2007, 27: 2856–2863 (in Chinese with English abstract)

[16]Delhaize E. Uptake on environmental stress:aluminum toxicity and tolerance in plants. Plant Physiol, 1995, 107: 315–321

[17]郑国红, 胡婵娜, 刘鹏. 外源钾对铁胁迫下水稻元素吸收运输规律的影响. 水土保持学报, 2010, 24(5): 141–145

Zheng G H, Hu C N, Liu P. Effects of exogenous kalium on elements absorption and transportation of rice under iron toxicity. J Soil Water Con, 2010, 24(5): 141–145 (in Chinese with English abstract)

[18]Peter R. Interaction between aluminum toxicity and calcium uptake at the root apex in near-isogenies lines of wheat (Triticum aestivan L.) differing in aluminum tolerance. Plant Physiol, 1993, 102: 975–982

[19]Drewm C. Oxygen deficiency and root metabolism: injury and acceliation under hypoxia and anoxia. Annu Rev Plant Physiol Mol Biol, 1997, 48: 223–250

[20]Link H R, Weng C H, Lo H F. Study of the root antioxidative system of tomatoes and eggplants under water logged conditions. Plant Sci, 2004, 167: 355–366

[21]Wang C, Zhang S H, Wang P F, Hou J, ZhangW J, LiW, Lin Z P. The effect of excess Zn onmineral nutrition and antioxidative response in rapeseed seedlings. Chemosphere, 2009, 75, 1468–1476

[22]周媛. 水杨酸对铝胁迫下栝楼生长的调控机理研究. 浙江师范大学硕士论文, 浙江金华, 2012

Zhou Y. The Mechanism of Salicylic Acid Regulating Aluminum Stress in Trichosathe skirilowii Maxim. MS Thesis of Zhejiang Normal University, Jinhua, China, 2012 (in Chinese with English abstract)
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