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作物学报 ›› 2016, Vol. 42 ›› Issue (08): 1247-1252.doi: 10.3724/SP.J.1006.2016.01247

• 研究简报 • 上一篇    下一篇

利用HMW-GS全缺失突变体快速构建Glu-1位点近等渗入系

张星星1,2,**,王召军2,3,**,杨玉双2,4,王道文2,郑文明1,*,董振营2,*   

  1. 1河南农业大学生命科学学院 / 小麦玉米作物学国家重点实验室/河南粮食作物协同创新中心, 河南郑州450002; 2中国科学院遗传与发育生物学研究所 / 植物细胞与染色体工程国家重点实验室, 北京100101; 3中国科学院大学, 北京100049; 4中国热带农业科学院橡胶研究所, 海南儋州571731
  • 收稿日期:2016-02-22 修回日期:2016-05-09 出版日期:2016-08-12 网络出版日期:2016-06-02
  • 通讯作者: 郑文明, E-mail: wmzheng@henau.edu.cn; 董振营, E-mail: zhydong@genetics.ac.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31300280)和国家重点基础研究发展计划(973计划)项目(2013CB127702)资助。

Rapid Development of Glu-1 Locus Near-isogenic Introgression Lines Using HMW-GS Deletion Mutant

ZHANG Xing-Xing1,2,**,WANG Zhao-Jun2,3,**,YANG Yu-Shuang2,4,WANG Dao-Wen2,ZHENG Wen-Ming1,*,DONG Zhen-Ying2,*   

  1. 1State Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops, College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2State Key Laboratory of PlantCell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; 3University of Chinese Academy of Sciences, Beijing 100049,China; 4Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571731, China
  • Received:2016-02-22 Revised:2016-05-09 Published:2016-08-12 Published online:2016-06-02
  • Contact: 郑文明, E-mail: wmzheng@henau.edu.cn; 董振营, E-mail: zhydong@genetics.ac.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31300280) and the National Key Basic Research Program of China (2013CB127702).

摘要:

小麦高分子量麦谷蛋白亚基(highmolecular weight glutenin subunit, HMW-GS)由Glu-A1Glu-B1Glu-D1位点中含有的复等位基因编码,评价和优化Glu-1位点组合是认识与改良HMW-GS表达与功能的重要途径。本研究创制了以小偃81为背景的HMW-GS基因完全缺失突变体DLGlu1。将DLGlu1与加拿大优质强筋小麦品种Glenlea杂交,结合后代幼胚培养与分子标记辅助选择技术,在BC3F3种子中快速鉴定出来自Glenlea的Glu-A1aGlu-B1alGlu-D1d位点不同组合的7种渗入系材料,可进一步发展成一套完整的Glu-1位点有差异的近等渗入系。本研究表明,DLGlu1可用于Glu-1位点近等渗入系的快速创制,对Glu-1位点功能研究和改良具有重要价值。

关键词: 小麦, 高分子量麦谷蛋白亚基, 缺失突变体, 渗入系

Abstract:

Wheat (Triticumaestivum L., AABBDD) highmolecular weight glutenin subunits (HMW-GS) were encoded by the genes located inGlu-A1, Glu-B1and Glu-D1 loci.Evaluation and optimization of the combination of HMW-GS are very importantto understand Glu-1functions. In this study, we constructed aHMW-GS deletion mutant, DLGlu1 withXiaoyan 81 background, and crossed it with Glenlea, a Canada elite wheat variety with superior end-use quality. Combining the technologies of wheat embryo culture and molecular marker-assisted selection (MAS), we obtained seven introgression lines containing GlenleaGlu-A1a, Glu-B1al, and Glu-D1d loci, which can be developed as a complete set of near-isogenic introgression lines possessingGlenleadifferentHMW-GS genes. Our study indicated that the Glu-1 deletion mutant DLGlu1is of great value in the fast development of Glu-1 near-isogenic introgression lines and the studyand utility of wheat Glu-1.

Key words: Wheat, HMW-GS, Deletion mutant, Introgression lines

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