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作物学报 ›› 2016, Vol. 42 ›› Issue (12): 1754-1763.doi: 10.3724/SP.J.1006.2016.01754

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻温敏型叶片白化突变体tsa1的表型鉴定和基因定位

刘钰龙1,**，刘峰1,**，周坤能3，苏晓妹1，方先文2，张云辉2,*，鲍依群1,*   

  1. 1南京农业大学生命科学学院,江苏南京210095;2江苏省农业科学院粮食作物研究所/江苏省农业种质资源保护与利用平台,江苏南京210014;3安徽省农业科学院水稻研究所,安徽合肥230031
  • 收稿日期:2016-03-21 修回日期:2016-07-11 出版日期:2016-12-12 网络出版日期:2016-08-24
  • 通讯作者: 鲍依群,E-mail:baoyiqun@njau.edu.cn,Tel:025-84396795;张云辉,E-mail:zyhrice@163.com,Tel:025-84390321
  • 基金资助:

    本研究由国家自然科学基金项目(31401036)，江苏省自然科学基金项目(BK20130706)，江苏省农业科技自主创新资金[CX(14)5005]，江苏省农业科学院基本科研业务专项[ZX(15)4015]和安徽省自然科学基金项目(1408085MKL62)资助。

Phenotypic Characterization and Gene Mapping of a Thermo-sensitive AlbinoLeaf Mutant tsa1 in Rice

LIU Yu-Long1,**,LIU Feng1,**,ZHOU Kun-Neng3,SU Xiao-Mei1,FANG Xian-Wen2,ZHANG Yun-Hui2,*,BAO Yi-Qun1,*   

  1. 1 College of Life Science, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Jiangsu Agricultural Germplasm Resources Conservation and Utilization Platform, Nanjing 210014, China; 3 Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
  • Received:2016-03-21 Revised:2016-07-11 Published:2016-12-12 Published online:2016-08-24
  • Contact: Bao Yiqun,E-mail:baoyiqun@njau.edu.cn,Tel:025-84396795;Zhang Yunhui,E-mail:zyhrice@163.com,Tel:025-84390321
  • Supported by:
    This study was supported by the National Natural Science Foundation (31401036), the Natural Science Foundation of Jiangsu Province (BK20130706), the Jiangsu Province Agricultural Science and Technology Independent Innovation Foundation [CX(14)5005], the Jiangsu Academy of Agricultural Sciences Fundamental Research Projects [ZX(15)4015], and the Natural Science Foundation of Anhui Province (1408085MKL62).

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摘要：

一个由甲磺酸乙酯(EMS)诱变宁粳36水稻品种获得的温敏型叶片白化突变体tsa1在低温条件下(20~24°C)表现叶片白化，但在较高温度下(28~32°C)叶色与野生型无显著差异。突变体白化叶片中叶绿素a和类胡萝卜素含量与野生型相比显著下降。显微观察发现突变体白化组织中正常叶绿体数量稀少，包含大量小型的异常叶绿体，进一步用透射电镜观察发现异常叶绿体中无发育完整的类囊体片层结构，表明该突变体中叶绿体发育存在严重缺陷。遗传分析表明该突变性状受单个隐性核基因控制。利用tsa1与南京11杂交所得的F2群体中的368个隐性极端个体，将该突变基因定位于第5染色体长臂163 kb的范围内。本研究为该基因的图位克隆及功能解析奠定了基础。

关键词: 水稻(Oryzasativa.L), 温敏型, 白化, 突变体, 叶绿体发育

Abstract:

A thermo-sensitive albino leaf mutant tsa1 was obtained from Ningjing36 induced by Ethyl methane sulfonate (EMS). The mutant developed albinotic leaves under low temperature(20-24°C), and the color of mutant leaves was comparable with the wild-type under high temperature (28-32°C). Compared with wild type, the chlorophyll a and carotene contents of mutant albinotic leaves obviously decreased under low temperature condition. With microscopeobservation, the quantity of normal chloroplast significantly decreased, besides, there were a large number of small abnormal chloroplasts in albinotic leaves. The abnormal chloroplast in albinotic tissue of mutant had no obvious thylakoid stack under transmission electron microscopy. These results indicate that tsa1 mutant has defect on chloroplast development under low temperature. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. Genetic mapping of the mutant gene was conducted using 368 recessive individuals from F2 population of tsa1/Nanjing 11, the target gene was finally located in a 163 kb region on the chromosome 5. This study provides a basis for map-based cloning and functional analysis of the target gene.

Key words: Rice(OryzasativaL.), Thermo-sensitive, Albino, Mutant, Chloroplastdevelopment

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