作物学报 ›› 2017, Vol. 43 ›› Issue (05): 691-700.doi: 10.3724/SP.J.1006.2017.00691
刘会云1,**,王婉晴2,**,李欣1,王轲1,王龙3,杜丽璞1,晏月明2,*,叶兴国1,*
LIU Hui-Yun1,**,WANG Wan-Qing2,**,LI Xin1, WANG Ke1,WANG Long3,DU Li-Pu1,YANG Yue-Ming2,*,YE Xing-Guo1,*
摘要:
高分子量谷蛋白亚基(HMW-GS)是小麦籽粒贮藏蛋白的重要成分,其组成、表达和含量决定面团弹性和加工品质。本研究以Glu-B1位点上1Bx20和1By20双亚基沉默的小麦无性系变异体AS208为材料,以AS208的来源亲本轮选987作为对照,对AS208中Glu-B1位点的沉默机制和1Bx20、1By20亚基沉默对种子中蛋白体融合以及合成加工相关基因表达的影响进行了研究。Southern blot分析发现AS208比轮选987少2条特异带,染色体原位杂交(FISH)结果显示轮选987中有6条染色体出现杂交信号,而在AS208中有4条染色体出现杂交信号,表明AS208基因组中缺失Glu-B1位点。透射电镜观察发现,与轮选987相比,AS208的籽粒蛋白体形成过程,以及蛋白体大小和形状基本一致。qRT-PCR检测发现,在12个与籽粒蛋白质合成与加工相关基因中,有7个的表达模式两品种相似,有8个的表达量在AS208中高于在轮选987中,特别是Bip、PDI-1和PDI-5基因(高1.5~2.0倍)。本研究结果表明,小麦Glu-B1位点的基因对种子中蛋白体的形成没有明显影响,但一定程度上刺激了多数蛋白质合成和加工相关基因的表达,保证了种子内蛋白含量、蛋白体外形和大小基本不变,表现负反馈调节效应。
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