甘蔗品种的AFLP和SSR标记鉴定及其应用

doi:10.3724/SP.J.1006.2018.00723

作物学报 ›› 2018, Vol. 44 ›› Issue (05): 723-736.doi: 10.3724/SP.J.1006.2018.00723

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

甘蔗品种的AFLP和SSR标记鉴定及其应用

汪洲涛, 游倩, 高世武, 王春风, 李竹, 马晶晶, 阙友雄, 许莉萍, 罗俊^*()

福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室, 福建福州 350002

收稿日期:2017-10-13 接受日期:2018-03-15 出版日期:2018-05-20 网络出版日期:2018-03-16
通讯作者: 罗俊
作者简介:
第一作者联系方式: E-mail: suiyiyixinyisi@163.com
基金资助:
本研究由国家现代农业产业技术体系建设专项(CARS-17)和引进国际先进农业科学技术计划(948计划)项目(2014-S18)资助

Identification of Sugarcane Varieties by AFLP and SSR Markers and Its Application

Zhou-Tao WANG, Qian YOU, Shi-Wu GAO, Chun-Feng WANG, Zhu LI, Jing-Jing MA, You-Xiong QUE, Li-Ping XU, Jun LUO^*()

Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

Received:2017-10-13 Accepted:2018-03-15 Published:2018-05-20 Published online:2018-03-16
Contact: Jun LUO
Supported by:
This study was supported by China Agriculture Research System (CARS-17) and the Program of Introducing International Super Agricultural Science and Technology (948 Program) (2014-S18).

摘要/Abstract

摘要：

品种遗传多样性和指纹图谱是育种、品种权保护和新品种推广等工作的重要参考和依据。本研究选用38个来自国家甘蔗品种区域试验的甘蔗新品种(系), 以9对AFLP标记扩增出348个位点, 多态性位点248个, 多态性比率为72.26%; 15对SSR标记扩增出180个位点, 多态性位点176个, 多态性比率为97.78%。38个新品种(系)的遗传相似系数分布在0.668~0.847之间, 其箱线图分布特征显示, 其中的FN、MT、YZ、YG、GT等系列品种(系)遗传基础相似。通过UPGMA聚类表明, 可在遗传相似系数为0.732处将38个甘蔗新品种(系)划分为2个群体, 其中福农09-2201和桂糖06-1492作为一个子群体最先被划分出来, 它们在群体中的异质性较强; 另外, 在遗传相似性系数为0.770处划分出一个子群体a, 其中包含参照品种ROC22、福农07-3206、福农40、海蔗22、桂糖09-12、柳城07-150等品种(系)。ROC22具有广适应性和高产高糖等优良特性, 子群体a中的另外几个品种(系)则更有可能拥有这些特性, 具有更高的推广潜力。本研究选择60个SSR位点构建了38个甘蔗新品种(系)的指纹图谱, 对品种鉴定及品种权的保护具有重要作用, 可望直接应用于指导甘蔗种质资源的遗传多样性评价和分子指纹图谱鉴定, 并将为这些品种(系)推广布局或作为杂交亲本利用提供参考和借鉴。

关键词: 甘蔗, 分子标记, 种质鉴定, 群体划分, 推广布局

Abstract:

Genetic diversity and fingerprint of varieties are crucial reference for breeding, cultivar right protection and new cultivar extension. We used nine pairs of AFLP markers and 15 pairs of SSR markers to scan 38 new sugarcane varieties (lines) from the national variety regional testings, obtaining abundant fingerprint data. Among the total 348 AFLP bands, 248 were polymorphic, with a polymorphism rate of 72.26 %. In addition, among the total 180 SSR bands, 176 were polymorphic, with a polymorphism rate of 97.78 %. The genetic similarity coefficients of these 38 new varieties (lines) were distributed from 0.668 to 0.847. We explored the distribution characteristics of genetic similarity coefficients using the boxplot, observing the genetic basis of five series (FN, MT, YZ, YG, and GT) of the 38 new sugarcane varieties (lines) was approximately similar. The clustering analysis manifested that these 38 new sugarcane varieties (lines) were divided into two groups at the genetic similarity coefficient of 0.732, with a subgroup including FN09-2201 and GT06-1492 that had high heterogeneity. Moreover, there was a small subgroup containing ROC22 at the genetic similarity coefficient of 0.770. Except for ROC22, the subgroup also contained FN 07-3206, FN 40, HZ 22, GT 09-12, and LC 07-150. ROC22 has wide adaptability, high yield, high sugar content and other excellent characteristics, other varieties (lines) in the same subgroup should be more likely to have these excellent characteristics and higher extention potential. Finally, through selection of 60 efficient amplification sites in the identification of SSR markers, we constructed the fingerprints of these 38 new sugarcane varieties (lines), which should play an important role in variety identification and variety protection. This study is expected to be directly applied to guide the genetic diversity assessment and molecular fingerprinting identification of sugarcane germplasm, and also to provide references for the extention and layout of these varieties or their utilization as hybrid parents.

Key words: sugarcane, molecular marker, germplasm identification, group division, variety extension

汪洲涛, 游倩, 高世武, 王春风, 李竹, 马晶晶, 阙友雄, 许莉萍, 罗俊. 甘蔗品种的AFLP和SSR标记鉴定及其应用[J]. 作物学报, 2018, 44(05): 723-736.

Zhou-Tao WANG, Qian YOU, Shi-Wu GAO, Chun-Feng WANG, Zhu LI, Jing-Jing MA, You-Xiong QUE, Li-Ping XU, Jun LUO. Identification of Sugarcane Varieties by AFLP and SSR Markers and Its Application[J]. Acta Agronomica Sinica, 2018, 44(05): 723-736.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2018.00723

https://zwxb.chinacrops.org/CN/Y2018/V44/I05/723

图/表 10

附表1

38份来自3轮国家甘蔗品种区域试验的品种(系)"

编号 No.	品种(系) Variety (line)	亲本组合 Parent combination	选育单位 Breeding institution	参试轮次 Round	系列 Series
1	福农07-2020 Funong 07-2020	粤糖91-976 × LC 85-384 Yuetang 91-976 × LC 85-384	福建农林大学甘蔗综合研究所 SIFAFU	10	FN
2	福农07-3206 Funong 07-3206	90-1211 × 77-797	福建农林大学甘蔗综合研究所 SIFAFU	10	FN
3	福农40 Funong 40	福农93-3406 × 粤糖91-976 Funong 93-3406 × Yuetang 91-976	福建农林大学甘蔗综合研究所 SIFAFU	10	FN
4	福农09-2201 Funong 09-2201	ROC 22 × 桂糖00-122 ROC 22 × Guitang 00-122	福建农林大学甘蔗综合研究所 SIFAFU	11	FN
5	福农09-7111 Funong 09-7111	桂糖96-44 × ROC 11 Guitang 96-44 × ROC 11	福建农林大学甘蔗综合研究所 SIFAFU	11	FN
6	福农09-12206 Funong 09-12206	CP 65-357 × 崖城97-40 CP 65-357 × Yacheng 97-40	福建农林大学甘蔗综合研究所 SIFAFU	12	FN
7	福农11-2105 Funong 11-2105	川蔗89-103 × 云瑞05-770 Chuanzhe 89-103 × Yunrui 05-770	福建农林大学甘蔗综合研究所 SIFAFU	12	FN
8	福农09-4095 Funong 09-4095	粤糖93-159 × 云蔗91-790 Yuetang 93-159 × Yunzhe 91-790	福建农林大学甘蔗综合研究所 SIFAFU	12	FN
9	闽糖06-1405 Mintang 06-1405	闽糖92-649 × ROC 10 Mintang 92-649 × ROC 10	福建省农业科学院甘蔗研究所 SRIFAAS	11	MT
10	闽糖07-2005 Mintang 07-2005	崖城73-512 × ROC 22 Yacheng 73-512 × ROC 22	福建省农业科学院甘蔗研究所 SRIFAAS	12	MT
11	闽糖09-104 Mintang 09-104	桂糖90-420 × ROC 10 Guitang 90-420 × ROC 10	福建省农业科学院甘蔗研究所 SRIFAAS	12	MT
编号 No.	品种(系) Variety (line)	亲本组合 Parent combination	选育单位 Breeding institution	参试轮次 Round	系列 Series
12	闽糖02-205 Mintang 02-205	崖城90-3 × ROC 10 Yacheng 90-3 × ROC 10	福建省农业科学院甘蔗研究所 SRIFAAS	10	MT
13	云蔗08-2060 Yunzhe 08-2060	粤糖93-159 × Q 121 Yuetang 93-159 × Q 121	云南省农业科学院甘蔗研究所 SRIYAAS	10	YZ
14	云蔗08-1095 Yunzhe 08-1095	CP 84 -1198 × 科5 CP 84-1198 × Ke5	云南省农业科学院甘蔗研究所 SRIYAAS	11	YZ
15	云蔗09-1028 Yunzhe 09-1028	云瑞15-178 × 闽糖86-2121 Yunrui 15-178 × Mintang 86-2121	云南省农业科学院甘蔗研究所 SRIYAAS	12	YZ
16	云蔗09-1601 Yunzhe 09-1601	CP 94-1100 × 川糖89-103 CP 94-1100 × Chuantang 89-103	云南省农业科学院甘蔗研究所 SRIYAAS	12	YZ
17	云瑞09-315 Yunrui 09-315	CL 69-52 × 云瑞05-285 CL 69-52 × Yunrui 05-285	云南省农业科学院甘蔗研究所 SRIYAAS	12	YZ
18	云瑞07-1433 Yunrui 07-1433	云瑞99-155 × L 75-20 Yunrui 99-155 × L75-20	云南省农业科学院甘蔗研究所 SRIYAAS	10	YZ
19	云瑞10-187 Yunrui 10-187	ROC 20 × 云瑞05-282 ROC20 × Yunrui 05-282	云南省农业科学院甘蔗研究所 SRIYAAS	12	YZ
20	云瑞10-701 Yunrui 10-701	云瑞08-18 × 云瑞05-704 Yunrui 08-18 × Yunrui 05-704	云南省农业科学院甘蔗研究所 SRIYAAS	12	YZ
21	德蔗07-36 Dezhe 07-36	桂糖92-66 × CP 67-412 Guitang 92-66 × CP 67-412	云南德宏州甘蔗科学研究所 SRIDY	11	DZ
22	德蔗09-78 Dezhe 09-78	桂糖94-119 × ROC 10 Guitang 94-119 × ROC 10	云南德宏州甘蔗科学研究所 SRIDY	12	DZ
23	德蔗09-84 Dezhe 09-84	桂糖94-119 × ROC 10 Guitang 94-119 × ROC 10	云南德宏州甘蔗科学研究所 SRIDY	12	DZ
24	粤甘43 Yuegan 43	粤糖93-213 × 粤糖93-159 Yuetang 93-213 × Yuetang 93-159	广州甘蔗糖业研究所 GSIRI	10	YG
25	粤甘46 Yuegan 46	粤糖00-236 × 桂糖96-211 Yuetang 00-236 × Guitang 96-211	广州甘蔗糖业研究所 GSIRI	10	YG
26	粤甘47 Yuegan 47	粤农73-204 × ROC 22 Yuenong 73-204 × ROC 22	广州甘蔗糖业研究所 GSIRI	11	YG
27	粤甘48 Yuegan 48	HOCP 95-988 × 粤糖97-76 HOCP 95-988 × Yuetang 97-76	广州甘蔗糖业研究所 GSIRI	12	YG
28	粤甘50 Yuegan 50	粤糖96-86 × 粤糖99-66 Yuetang 96-86 × Yuetang 99-66	广州甘蔗糖业研究所 GSIRI	12	YG
29	海蔗22 Haizhe 22	粤糖93-159 × ROC 22 Yuetang 93-159 × ROC 22	广州甘蔗糖业研究所 GSIRI	11	YG
30	桂糖06-2081 Guitang 06-2081	桂糖00-122 × 崖城97-47 Guitang 00-122 × Yacheng 97-47	广西农业科学院甘蔗研究所 SRIGAAS	11	GT
31	桂糖08-1180 Guitang 08-1180	ROC 26 × ROC 22	广西农业科学院甘蔗研究所 SRIGAAS	11	GT
32	桂糖06-1492 Guitang 06-1492	CP 72-1210 × 湛蔗92-126 CP 72-1210 × Zhanzhe 92-126	广西农业科学院甘蔗研究所 SRIGAAS	12	GT
33	桂糖09-12 Guitang 09-12	ROC 24 × 粤农79-780 ROC 24 × Yuenong 79-780	广西农业科学院甘蔗研究所 SRIGAAS	12	GT
编号 No.	品种(系) Variety (line)	亲本组合 Parent combination	选育单位 Breeding institution	参试轮次 Round	系列 Series
34	柳城07-500 Liucheng 07-500	粤糖92-1287 × CP 72-1210 Yuetang 92-1287 × CP 72-1210	柳城县甘蔗研究中心 SRCLC	10	LC
35	柳城07-150 Liucheng 07-150	粤糖85-177 × ROC 22 Yuetang 85-177 × ROC 22	柳城县甘蔗研究中心 SRCLC	11	LC
36	柳城07-506 Liucheng 07-506	粤糖85-177 × ROC 22 Yuetang 85-177 × ROC 22	柳城县甘蔗研究中心 SRCLC	12	LC
37	赣蔗07-538 Ganzhe 07-538	ROC 10 × CP 57-614	江西省甘蔗研究所 SRIJP	10	GZ
38	ROC 22		台湾糖业研究所 SPRIT	参照	ROC

附表1

表1

表2

图1

图2

表3

图3

图4

图5

图6

参考文献 55

[1]	Menhas R, Umer S, Shabbir G.Climate change and its impact on food and nutrition security in Pakistan.Iran J Public Health, 2016, 45: 549-550 pmid: 4888188
[2]	Kumar M.Impact of climate change on crop yield and role of model for achieving food security.Environ Monit Assess, 2016, 188: 1-14 doi: 10.1007/s10661-015-4999-z
[3]	Tiepolo M.Urbanization and food security in Niamey, Niger.Stor Urbana, 2002, 26: 29-58 pmid: 17500125
[4]	Matsumoto K.Energy structure and energy security under climate mitigation scenarios in China.PLoS One, 2015, 10: e0144884 doi: 10.1371/journal.pone.0144884 pmid: 4684348
[5]	Luo J, Pan Y B, Xu L P, Grisham M P, Zhang H, Que Y X.Rational regional distribution of sugarcane cultivars in China.Sci Rep, 2015, 5: 15721 doi: 10.1038/srep15721 pmid: 26499905
[6]	Que Y X, Pan Y B, Lu Y H, Yang C, Yang Y T, Huang N, Xu L P.Genetic analysis of diversity within a Chinese local sugarcane germplasm based on start codon targeted polymorphism.Biomed Res Int, 2014, (5): 468375 doi: 10.1155/2014/468375 pmid: 24779012
[7]	袁隆平. 杂交水稻超高产育种. 杂交水稻, 1997, 12(6): 1-3
	Yuan L P.Hybrid rice breeding for super high yield.Hybrid Rice, 1997, 12(6): 1-3 (in Chinese with English abstract)
[8]	Tang S X, Wei X H, Javier E L.Introduction and utilization of INGER rice germplasm in China.Agric Sci China, 2004, 3: 561-567
[9]	Erskine W, Muehlbauer F J.Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm.Theor Appl Genet, 1991, 83: 119-125 doi: 10.1007/BF00229234 pmid: 24202265
[10]	Nayak S, Naik P K, Acharya L, Mukherjee A K, Panda P C, Das P.Assessment of genetic diversity among 16 promising cultivars of ginger using cytological and molecular markers.Z Naturforsch C, 2005, 60: 485-492 doi: 10.1038/ncprheum0465 pmid: 16047412
[11]	Wang C L, Singh D, Mitra S K.Biochemical markers: A useful tool for assessing genetic diversity in jackfruit (Artocarpus heterophyllus Lam.). Acta Hortic, 2011, 890: 91-101
[12]	Li H, Cao S Y, Niu J, Yuan P, Zhao D.The types and application of molecular markers in the study of pomegranate germplasm resources. Acta Hortic, 2015, 1089: 127-132 doi: 10.17660/ActaHortic.2015.1089.14
[13]	Praveen K, Kumar M H, Umamaheshwari A, Reddy D M, Sudhakar P, Sabita N.SGDB: a sugarcane germplasm database.Sugar Tech, 2015, 17: 150-155 doi: 10.1007/s12355-014-0307-4
[14]	Chen R K, Xu L P, Lin Y Q.Modern Sugarcane Genetic Breeding. Beijing: China Agriculture Press, 2011. pp 2-12
[15]	刘庆昌. 遗传学. 北京:科学出版社, 2015. pp 286-288
	Liu Q C.Genetics. Beijing: Science Press, 2015. pp 286-288 (in Chinese)
[16]	Ashfaq M, Khan A S.Genetic diversity in basmati rice (Oryza sativa L.) germplasm as revealed by microsatellite (SSR) markers. Russ J Genet, 2012, 48: 53-62 doi: 10.1134/S1022795411120027 pmid: 22567855
[17]	Shoaib A, Arabi M I E. Genetic diversity among syrian cultivated and landraces wheat revealed by AFLP markers.Genet Resour Crop Evol, 2006, 53: 901-906 doi: 10.1007/s10722-004-3557-2
[18]	Lenka D, Tripathy S K, Kumar R, Behera M, Ranjan R.Assessment of genetic diversity in quality protein maize (QPM) inbreds using ISSR markers.J Environ Biol, 2015, 36: 985-992 pmid: 26364479
[19]	Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M. AFLP: a new technique for DNA fingerprinting.Nucl Acids Res, 1995, 23: 4407-4414 doi: 10.1093/nar/23.21.4407 pmid: 7501463
[20]	Liu X L, Li X J, Xu C H, Lin X Q, Deng Z H.Genetic diversity of populations of Saccharum spontaneum, with different ploidy levels using SSR molecular markers. Sugar Tech, 2016, 18: 365-372 doi: 10.1007/s12355-015-0399-5
[21]	Lander E S.The new genomics: global views of biology.Science, 1996, 274: 536-539 doi: 10.1126/science.274.5287.536
[22]	Zietkiewicz E, Rafalski A, Labuda D.Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification.Genomics, 1994, 20: 176-183 doi: 10.1006/geno.1994.1151 pmid: 8020964
[23]	Duarte Filho L S C, Silva P P, Santos J M, Barbosa G V S, Ramalho-Neto C E, Soares L, Andrade J C F, Almeida C. Genetic similarity among genotypes of sugarcane estimated by SSR and coefficient of parentage.Sugar Tech, 2010, 12: 145-149 doi: 10.1007/s12355-010-0028-2
[24]	Russell J R, Fuller J D, Macaulay M, Hatz B G, Jahoor A, Powell W, Waugh R.Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs.Theor Appl Genet, 1997, 95: 714-722 doi: 10.1007/s001220050617
[25]	Pejic I, Ajmone-Marsan P, Morgante M, Kozumplick V, Castiglioni P, Taramino G, Motto M.Comparative analysis of genetic similarity among maize inbred lines detected by RFLPs, RAPDs, SSRs, and AFLPs.Theor Appl Genet, 1998, 97: 1248-1255 doi: 10.1007/s001220051017
[26]	Guichoux E, Lagache L, Wagner S, Chaumeil P, Léger P, Lepais O O, Lepoittevin C, Malausa T, Revardel E, Salin F, Petit R J.Current trends in microsatellite genotyping.Mol Ecol Resour, 2011, 11: 591-611 doi: 10.1111/j.1755-0998.2011.03014.x pmid: 21565126
[27]	刘新龙, 蔡青, 毕艳, 陆鑫, 马丽, 应雄美. 中国滇蔗茅种质资源遗传多样性的AFLP分析. 作物学报, 2009, 35: 262-269 doi: 10.3724/SP.J.1006.2009.00262
	Liu X L, Cai Q, Bi Y, Lu X, Ma L, Ying X M.Genetic diversity analysis for germplasm of Erianthus rockii in China. Acta Agron Sin, 2009, 35: 262-269 (in Chinese with English abstract) doi: 10.3724/SP.J.1006.2009.00262
[28]	Lima M L, Garcia A A, Oliveira K M, Matsuoka S, Arizono H, De Souza C L Jr, De Souza A P. Analysis of genetic similarity detected by AFLP and coefficient of parentage among genotypes of sugar (Saccharum spp.). Theor Appl Genet, 2002, 104: 30-38
[29]	Barret B A, Kidwell K K.Comparison of AFLP and pedigree-based genetic diversity assessment methods using wheat cultivars from the Pacific Northwest.Crop Sci, 1998, 38: 1271-1278 doi: 10.2135/cropsci1998.0011183X003800050026x
[30]	Singh R K, Singh R B, Singh S P, Mishra N, Rastogi J, Sharma M L, Kumar A.Genetic diversity among Saccharum spontaneum clones and commercial hybrids through SSR markers. Sugar Tech, 2013, 15: 109-115
[31]	齐永文, 劳方业, 张垂明, 樊丽娜, 何慧怡, 刘少谋, 李奇伟, 邓海华. 中美重要甘蔗种质SSR遗传多样性比较. 热带作物学报, 2011, 32: 99-104 doi: 10.3969/j.issn.1000-2561.2011.01.021
	Qi Y W, Lao F Y, Zhang C M, Fan L N, He H Y, Liu S M, Li Q W, Deng H H.Comparative analysis of genetic diversity of Chinese and American sugarcane (Saccharum spp.) using SSR makers. Chin J Trop Crop, 2011, 32: 99-104 (in Chinese with English abstract) doi: 10.3969/j.issn.1000-2561.2011.01.021
[32]	刘新龙, 马丽, 陈学宽, 应雄美, 蔡青, 刘家勇, 吴才文. 云南甘蔗自育品种DNA指纹身份证构建. 作物学报, 2010, 36: 202-210 doi: 10.3724/SP.J.1006.2010.00202
	Liu X L, Ma L, Chen X K, Ying X M, Cai Q, Liu J Y, Wu C W.Establishment of DNA fingerprint ID in sugarcane cultivars in Yunnan, China.Acta Agron Sin, 2010, 36: 202-210 (in Chinese with English abstract) doi: 10.3724/SP.J.1006.2010.00202
[33]	姚春雪, 王先宏, 何丽莲, 李富生. 甘蔗与蔗茅杂交不同世代的SSR指纹图谱构建. 分子植物育种, 2011, 9: 381-389 doi: 10.3969/mpb.009.000381
	Yao C X, Wang X H, He L L, Li F S.DNA fingerprint construction of different generations of Saccharum spp. × Erianthus fulvus using SSR marker. Mol Plant Breed, 2011, 9: 381-389 (in Chinese with English abstract) doi: 10.3969/mpb.009.000381
[34]	汪洲涛, 苏炜华, 阙友雄, 许莉萍, 张华, 罗俊. 应用AMMI和HA-GGE双标图分析甘蔗品种产量稳定性和试点代表性. 中国生态农业学报, 2016, 24: 790-800 doi: 10.13930/j.cnki.cjea.151284
	Wang Z T, Su W H, Que Y X, Xu L P, Zhang H, Luo J.Analysis of yield stability and test site representativeness of sugarcane trials using combined AMMI and HA-GGE biplot models.Chin J Eco-Agric, 2016, 24: 790-800 (in Chinese with English abstract) doi: 10.13930/j.cnki.cjea.151284
[35]	罗俊, 邓祖湖, 阙友雄, 袁照年, 陈如凯. 国家甘蔗第七轮区域试验品种的丰产性及稳定性. 应用与环境生物学报, 2012, 18: 734-739 doi: 10.3724/sp.j.1145.2012.00734
	Luo J, Deng Z H, Que Y X, Yuan Z N, Chen R K.Productivity and stability of sugarcane varieties in the 7th round national regional trial of China.Chin J Appl Environ Biol, 2012, 18: 734-739 (in Chinese with English abstract) doi: 10.3724/sp.j.1145.2012.00734
[36]	Luo J, Pan Y B, Que Y X, Zhang H, Grisham M P, Xu L P.Biplot evaluation of test environments and identification of mega-environment for sugarcane cultivars in China.Sci Rep, 2015, 5: 15505 doi: 10.1038/srep15505 pmid: 26489689
[37]	Pan Y B. Application of microsatellite and RAPD fingerprints in the Florida sugarcane variety program. Int Sugar J, 2003, March/April: 19-28
[38]	Pan Y B, Scheffler B E, Richard Jr E P. High-throughput molecular genotyping of commercial sugarcane clones with microsatellite (SSR) markers.Sugar Tech, 2007, 9: 176-181
[39]	Pan Y B.Databasing molecular identities of sugarcane (Saccharum spp.) clones constructed with microsatellite (SSR) DNA markers. Am J Plant Sci, 2010, 1: 87-94 doi: 10.4236/ajps.2010.12011
[40]	闫学兵, 阙友雄, 许莉萍, 郭晋隆, 陈如凯, 潘永保. 甘蔗EST序列的SSR信息分析. 热带作物学报, 2010, 31: 1497-1501
	Yan X B, Que Y X, Xu L P, Guo J L, Chen R K, Pan Y B.Analysis of SSR information in EST resources of sugarcane.Chin J Trop Crop, 2010, 31: 1497-1501 (in Chinese with English abstract)
[41]	Cordeiro G M, Pan Y B, Henry R J.Sugarcane microsatellites for the assessment of genetic diversity in sugarcane germplasm.Plant Sci, 2003, 165: 181-189 doi: 10.1016/S0168-9452(03)00157-2
[42]	Aitken K S, Jackson P A, McIntyre C L. A combination of AFLP and SSR markers provides extensive map coverage and identification of homo(eo)logous linkage groups in a sugarcane cultivar.Theor Appl Genet, 2005, 110: 789-801 doi: 10.1007/s00122-004-1813-7 pmid: 15700149
[43]	Milbourne D, Meyer R, Bradshaw J E, Baird E, Bonar N, Provan J, Powell W, Waugh R.Comparison of PCR-based marker systems for the analysis of genetic relationships in cultivated potato.Mol Breed, 1997, 3: 127-136 doi: 10.1023/A:1009633005390
[44]	Streit M, Gehlenborg N.Bar charts and box plots.Nat Methods, 2014, 11: 117 doi: 10.1038/nmeth.2807 pmid: 24645191
[45]	Nuzzo R L.The Box plots alternative for visualizing quantitative data.PM&R, 2016, 8: 268-272 doi: 10.1016/j.pmrj.2016.02.001 pmid: 26892802
[46]	Creste S, Sansoli D M, Tardiani A C S, Silva D N, Goncalves F K, Favero T M, Medeiros C N F, Festucci C S, Carlini-Garcia L A, Landell M G A, Pinto L R. Comparison of AFLP, TRAP and SSRs in the estimation of genetic relationships in sugarcane.Sugar Tech, 2010, 12: 150-154 doi: 10.1007/s12355-010-0029-1
[47]	Mohamed H A, Manosh K B, Zhang Z W, Guo W W.Exploitation of SSR, SRAP and CAPS-SNP markers for genetic diversity of citrus germplasm collection.Sci Hortic, 2011, 128: 220-227 doi: 10.1016/j.scienta.2011.01.021
[48]	高伟, 王坤波, 刘方, 王春英, 张香娣, 王玉红, 黎绍惠. SSR引物及多态性位点数对陆地棉野生种系聚类结果的影响. 植物遗传资源学报, 2013, 14: 237-242 doi: 10.3969/j.issn.1672-1810.2013.02.008
	Gao W, Wang K B, Liu F, Wang C Y, Zhang X D, Wang Y H, Li S H.Effection of the quantity of SSR primer and allele on cluster analysis of Gossypium hirsutum Linn races. J Plant Genet Resour, 2013, 14: 237-242 (in Chinese with English abstract) doi: 10.3969/j.issn.1672-1810.2013.02.008
[49]	Luo J, Pan Y B, Xu L P, Zhang H, Yuan Z N, Deng Z H, Chen R K, Que Y X.Cultivar evaluation and essential test locations identification for sugarcane breeding in China. Sci World J, 2014: 302753 doi: 10.1155/2014/302753 pmid: 4055468
[50]	Benin G, Matei G, Costa d O A, Silva G O, Hagemann T R, Lemes da Silva C, Pagliosa E S, Beche E. Relationships between four measures of genetic distance and breeding behavior in spring wheat.Genet Mol Res, 2012, 11: 2390-2400 doi: 10.4238/2012.June.15.3 pmid: 22782625
[51]	Yao J, Li H, Ye J, Shi L L.Relationship between parental genetic distance and offspring’s heterosis for early growth traits in Liriodendron: implication for parent pair selection in cross breeding.New For, 2016, 47: 163-177 doi: 10.1007/s11056-015-9508-2
[52]	Longin F H, Liu W X, Ranc N, Reif J C.Association of progeny variance and genetic distances among parents and implications for the design of elite maize breeding programs.Maydica, 2011, 56: 227-231 doi: 10.1007/s10343-010-0235-5
[53]	Wegary D, Vivek B, Labuschagne M.Association of parental genetic distance with heterosis and specific combining ability in quality protein maize.Euphytica, 2013, 191: 205-216 doi: 10.1007/s10681-012-0757-2
[54]	戎俊, 杨小强, 耿宇鹏, 宋志平, 卢宝荣. 分子生态学(第2版). 北京: 高等教育出版社, 2015. pp 249-250
	Rong J, Yang X Q, Geng Y P, Song Z P, Lu B R. Molecular Ecology (2nd edn). Beijing: Higher Education Press, 2015. pp 249-250 (in Chinese)
[55]	朱有勇. 农业生物多样性与作物病虫害控制. 北京: 科学出版社, 2013. pp 9-27
	Zhu Y Y.Agricultural Biodiversity and Control of Crop Diseases and Insect Pests. Beijing: Science Press, 2013. pp 9-27 (in Chinese)

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

组合 Combination	引物名 Primer name	序列 Sequence (5°-3°)	引物名 Primer name	序列 Sequence (5°-3°)
1	E-ACA	GACTGCGTACCAATTC ACA	M-CTG	GATGAGTCCTGAGTAA CTG
2	E-ACT	GACTGCGTACCAATTC ACT	M-CAA	GATGAGTCCTGAGTAA CAA
3	E-AAC	GACTGCGTACCAATTC AAC	M-CAC	GATGAGTCCTGAGTAA CAC
4	E-ACT	GACTGCGTACCAATTC ACT	M-CAG	GATGAGTCCTGAGTAA CAG
5	E-ACA	GACTGCGTACCAATTC ACA	M-CAC	GATGAGTCCTGAGTAA CTC
6	E-ACT	GACTGCGTACCAATTC ACT	M-CAC	GATGAGTCCTGAGTAA CAC
7	E-AAC	GACTGCGTACCAATTC AAC	M-CAG	GATGAGTCCTGAGTAA CAG
8	E-ACT	GACTGCGTACCAATTC ACT	M-CTC	GATGAGTCCTGAGTAA CTC
9	E-ACC	GACTGCGTACCAATTC ACC	M-CAG	GATGAGTCCTGAGTAA CAG

引物名 Primer name	重复单元 Repeating unit	引物序列(正/反) Primer sequence (Positive/Reverse) (5°-3°)	退火温度 T_m(°C)
SMC334BS	(TG)₃₆	CAATTCTGACCGTGCAAAGAT/CGATGAGCTTGATTGCGAATG	60
SMC336BS	(TG)₂₃(AG)₁₉	ATTCTAGTGCCAATCCATCTCA/CATGCCAACTTCCAAACAGAC	62
SMC286CS	(TG)₄₃	TCAAATGGGACCTTATTGGAG/TCCCTCGATCTCCGTTGTT	58
SMC569CS	(TG)₃₇	GCGATGGTTCCTATGCAACTT/TTCGTGGCTGAGATTCACACTA	62
SMC119CG	(TTG)₁₂	TTCATCTCTAGCCTACCCCAA/AGCAGCCATTTACCCAGGA	58
SMC31CUQ	(TC)₁₀(AC)₂₂	CATGCCAACTTCCAATACAGACT/AGTGCCAATCCATCTCAGAGA	62
mSSCIR43	(GT)₃(AT)₂(GT)₂₉	ATTCAACGATTTTCACGAG/AACCTAGCAATTTACAAGAG	52
SEP17	(CCT)₉	ACCCTGCTGGTCTCCTCC/ACGTTCGACGTCGTGTAGTG	60
SEP23	(AGA)₉	GTGTTCAGGCAGATGGTCCT/GTCGATGGCACCGATTTATT	60
SEP59	(CGG)₉	TTCCATTTACTCCTCCGTGC/CTCCCCCTCCTCGTACTTGT	60
SEP6	(GCA)₅(ACA)₁₃	CAGCCCATTAACCAAGCAAT/GAAGCAGCTGTTGCTCACTG	60
SEP70	(GCG)₈	AACTCACCCAACAAAGCGAC/AGACGAAGAGCTCGTGGATG	60
SEP8	(CT)₁₇	CTTGCTTCCCCTTTACTCCC/GAGGCGCCTTACTGTTCTTG	60
SEP84	(GGT)₉	AGAGACCGTAATGGTGACCG/ACCACCACCACCACCATACT	60
SEP89	(CGT)₁₀	AGCTCTGATTTTTGGGGGTT/GGAAGACAGTGGACGAGGTC	60

标记类别 Marker sort	引物名 Primer name	多态性位点数 Number of polymorphic loci	总扩增位点数 Total site number	多态比例 Polymorphic percentage (%)	引物多态信息含量 PIC
SSR标记 SSR marker (100-350 bp)	SEP6	9	9	100	0.964
	SPE8	13	14	92.86	0.959
	SEP17	10	10	100	0.961
	SEP23	22	22	100	0.979
	SEP59	10	11	90.91	0.920
	SEP70	8	8	100	0.895
	SEP84	5	5	100	0.695
	SEP89	12	12	100	0.896
	SMC334BS	10	12	83.33	0.927
	SMC336BS	12	12	100	0.967
	SMC286CS	20	20	100	0.961
	SMC569CS	9	9	100	0.779
	SMC119CG	13	13	100	0.961
	SMC31CUQ	11	11	100	0.967
	mSSCIR43	12	12	100	0.973
	总数/平均数Total/average	176	180	97.78
标记类别 Marker sort	引物名 Primer name	多态性位点数 Number of polymorphic loci	总扩增位点数 Total site number	多态比例 Polymorphic percentage (%)	引物多态信息含量 PIC
AFLP标记 AFLP marker (1-500 bp)	E-ACA/M-CTG	37	58	63.79	0.974
	E-ACT/M-CAA	24	42	57.14	0.968
	E-AAC/M-CAC	26	36	72.22	0.968
	E-ACT/M-CAG	24	32	75.00	0.971
	E-ACA/M-CAG	22	27	81.48	0.968
	E-ACT/M-CAC	19	25	76.00	0.972
	E-AAC/M-CAC	29	37	78.38	0.972
	E-ACT/M-CTC	36	51	70.59	0.974
	E-ACC/M-CAG	31	40	77.50	0.974
	总数/平均数Total/average	248	348	71.26	0.971

甘蔗品种的AFLP和SSR标记鉴定及其应用

Identification of Sugarcane Varieties by AFLP and SSR Markers and Its Application

RichHTML

PDF

摘要/Abstract

引用本文

使用本文

图/表 10

参考文献 55

相关文章 15

Metrics

本文评价

推荐阅读 0

关于本刊

在线期刊

作者中心

相关单位

联系我们

[1]	崔致远, 秦晨展, 刘星雨, 张海, 曾康, 黄国强, 徐景升. 甘蔗类四跨膜蛋白ScTSPAN18与6K2互作应答SCMV侵染研究[J]. 作物学报, 2026, 52(6): 1618-1630.
[2]	翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1、Pod-D1和Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[3]	彭佳泺, 李颖, 李丹丹, 杨军宁, 郭学峰, 张文姣, 俞晓雪, 周亚荣, 王振玉, 王彩香, 马雄风, 宿俊吉. 陆地棉I类LBD家族成员鉴定及GhLBD6调控开花期的功能和单倍型分析[J]. 作物学报, 2026, 52(6): 1682-1697.
[4]	张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[5]	田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[6]	王楚锐, 李开祥, 赵志, 肖麓, 唐国永, 赵志刚, 徐亮, 杜德志, 柳海东. 甘蓝型春油菜早花基因BnCRY2功能位点KASP标记的开发及应用[J]. 作物学报, 2026, 52(3): 708-721.
[7]	杨宗桃, 杨婷, 王禹童, 艾静, 李燕烨, 刘家勇, 邓军, 赵勇, 张跃彬. 甘蔗CLC基因家族鉴定与表达分析[J]. 作物学报, 2026, 52(3): 722-734.
[8]	王粤生, 葛冬冬, 程兰斐, 陈春环, 王长有, 刘新伦, 李停栋, 邓平川, 吉万全, 赵继新. 小麦-华山新麦草二体异代换系16DH25-7的分子细胞遗传学及抗病性鉴定[J]. 作物学报, 2026, 52(2): 433-445.
[9]	万慧兰, 吴华英, 曾丹, 钱禛锋, 赵昌祖, 廖然超, 何丽莲, 李富生. 蔗茅耐寒相关基因EfWRKY51克隆分析及功能验证[J]. 作物学报, 2025, 51(8): 2048-2059.
[10]	展宗冰, 靳奇峰, 刘迪, 吕迎春, 郭莹, 张雪婷, 虎梦霞, 王尚, 杨芳萍. 甘肃省小麦农家种老芒麦分子鉴定及其重要性状评价[J]. 作物学报, 2025, 51(3): 609-620.
[11]	徐晓伟, 冯晶, 王凤涛, 童朝阳, 张建周, 李春盈, 蔺瑞明. 小麦地方品种蚕老麦成株抗条锈病QTL定位[J]. 作物学报, 2025, 51(11): 2933-2943.
[12]	田汉钊, 冯龙婷, 应开, 孟天琪, 武军, 刘玉秀. 外引小麦种质麦谷蛋白亚基组成及评价[J]. 作物学报, 2025, 51(10): 2663-2680.
[13]	匡博文, 韦妳, 刘金典, 陈美燕, 毛兴洁, 段维兴, 杨细平. 基于甘蔗及其近缘属参考基因组开发SSR标记及数据库[J]. 作物学报, 2025, 51(1): 103-116.
[14]	刘鑫源, 程宇坤, 王丽丽, 战帅帅, 马孟瑶, 郭玲, 耿洪伟. 新疆小麦过氧化物酶活性基因TaPod-A1、TaPod-A3和TaPod-D1等位变异及分布规律[J]. 作物学报, 2025, 51(1): 68-78.
[15]	艾莎, 李莎, 方治伟, 李论, 李甜甜, 高利芬, 陈利红, 肖华锋, 万人静, 闫多子, 武星廷, 彭海, 韩瑞玺, 周俊飞. 棉花MNP标记位点开发及其在DNA指纹图谱构建中的应用[J]. 作物学报, 2024, 50(9): 2267-2278.