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作物学报 ›› 2024, Vol. 50 ›› Issue (10): 2493-2502.doi: 10.3724/SP.J.1006.2024.42007

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

深水稻全生育期耐盐性状的QTL定位

夏秀忠1,**(), 张宗琼1,**(), 农保选1, 冯锐1, 郭辉1, 陈灿1, 梁树辉1, 荘洁1, 廖祖宇1, 宋国显2, 杨行海1,*(), 李丹婷1,*()   

  1. 1广西壮族自治区农业科学院水稻研究所 / 广西水稻遗传育种重点实验室, 广西南宁 530007
    2钦州市农业科学研究所, 广西钦州 535000
  • 收稿日期:2024-01-31 接受日期:2024-06-20 出版日期:2024-10-12 网络出版日期:2024-07-08
  • 通讯作者: *李丹婷, E-mail: ricegl@163.com;杨行海, E-mail: yangxinghai514@163.com
  • 作者简介:夏秀忠, E-mail:xiaxiuzhong@163.com;
    张宗琼, E-mail:zhangzongqiong@gxaas.net
    **同等贡献
  • 基金资助:
    国家重点研发计划项目(2021YFD1200505-2);国家自然科学基金项目(32260487);国家自然科学基金项目(32360519);广西科技重大专项(桂科AA23073004);广西科技计划项目(桂科AB21238009);广西农业科学院基本科研业务专项(桂农科2021YT030)

QTL mapping for salt tolerance traits throughout the entire growth period of deep-water rice

XIA Xiu-Zhong1,**(), ZHANG Zong-Qiong1,**(), NONG Bao-Xuan1, FENG Rui1, GUO Hui1, CHEN Can1, LIANG Shu-Hui1, ZHUANG Jie1, LIAO Zu-Yu1, SONG Guo-Xian2, YANG Xing-Hai1,*(), LI Dan-Ting1,*()   

  1. 1Rice Research Institute, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning 530007, Guangxi, China
    2Qinzhou Institute of Agricultural Sciences, Qinzhou 535000, Guangxi, China
  • Received:2024-01-31 Accepted:2024-06-20 Published:2024-10-12 Published online:2024-07-08
  • Contact: *E-mail: ricegl@163.com;E-mail: yangxinghai514@163.com
  • About author:
    **(Contributed equally to this work)
  • Supported by:
    National Key Research and Development Program of China(2021YFD1200505-2);National Natural Science Foundation of China(32260487);National Natural Science Foundation of China(32360519);Guangxi Science and Technology Major Program(Gui Ke AA23073004);Guangxi Science and Technology Program(Gui Ke AB21238009);Special Funds of Basic Scientific Research Foundation of Guangxi Academy of Agricultural Sciences(Gui Nong Ke 2021YT030)

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摘要：

盐胁迫是许多沿海地区水稻生产的主要制约因素, 尤其是沿海地区的咸淡水交汇区域。耐盐性是一种复杂的性状, 可以通过QTL定位来帮助耐盐育种, 以培育更高耐盐性的水稻品种。本研究供体亲本为沿海深水稻品种赤禾, 受体亲本为美国水稻品种Lemont, 杂交获得174份F9代的重组自交系, 在芽期、苗期和生殖生长期分别利用浓度为15 g L-1、5 g L-1和5~6 g L-1的NaCl进行胁迫, 通过芽期相对发芽率、苗期耐盐性评级和生殖生长期的7个表型性状为基础数据, 利用142个SSR分子标记绘制连锁遗传图并进行QTL分析。鉴定结果发现, 赤禾在芽期表现敏盐, 在苗期和生殖生长期表现耐盐; Lemont相反。3个生长时期分别有70.11%、50.57%和60.34%的品系表现为弱耐盐性, 而且耐盐性为弱的负相关。本研究共鉴定出33个LOD值为2.52~10.32的QTL, 解释0.06%~13.68%的表型遗传变异, 解释最大遗传变异的QTL均由耐盐亲本贡献, 其中芽期4个、苗期6个和生殖生长期23个位点, 并在生殖生长期发现4个QTL重叠区域。这些QTL可以进一步研究, 不仅为提高水稻育种的耐盐性提供了新的遗传资源, 还有助于在水稻耐盐育种中, 提高水稻品种的耐盐性。

关键词: 全生育期, 耐盐, 深水稻, 鉴定评价, 数量性状位点

Abstract:

Salinity stress is a major constraint to rice production in many coastal regions, particularly in the salt-fresh water interface areas. Salt tolerance in rice is a complex trait that can be localized through quantitative trait loci (QTL) mapping, which can facilitate the breeding of rice varieties with enhanced salt tolerance. In this study, an F9 mapping population consisting of 174 recombinant inbred lines was developed from a cross between the coastal deep-water rice variety Chihe (donor parent) and the U.S. rice variety Lemont (receptor parent). The population was subjected to salinity stress during the germination, seedling, and reproductive stages with NaCl concentrations of 15 g L-1, 5 g L-1, and 5-6 g L-1, respectively. Data were collected on relative germination rates, seedling salt tolerance grades, and seven phenotypic traits during the reproductive stages. Linkage genetic mapping and QTL mapping were performed using 142 simple sequence repeat (SSR) markers. The results indicated that Chihe was salt-sensitive at the germination stage but exhibited salt tolerance at the seedling and reproductive stages, while Lemont lines were consistently salt-sensitive. Approximately 70.11%, 50.57%, and 60.34% of the lines showed salt sensitivity in the germination, seedling, and reproductive stages, respectively, with a weak negative correlation of salt tolerance across the stages. A total of 33 QTLs were identified, with LOD values ranging from 2.52 to 10.32 and phenotypic variation explained ranging from 0.06% to 13.68%. Specifically, 4 QTLs were identified for the germination stage, 6 for the seedling stage, and 23 for the reproductive stage. Four overlapping QTLs were identified at the reproductive stage, and the QTLs contributing to the largest phenotypic variation were all derived from the salt-tolerant parent. Further investigation of these QTLs can provide new genetic resources for improving salt tolerance in rice breeding, thereby aiding the development of rice varieties with enhanced tolerance to salinity stress.

Key words: whole growth period, salt tolerance, deep-water rice, identification and evaluation, quantitative trait locus (QTL)

图1

174份重组自交系芽期耐盐鉴定结果分布 A: 正常处理与盐胁迫下, 群体发芽率分布小提琴图; B: 芽期耐盐等级评价结果。***代表P < 0.001水平差异显著, 图基检验计算P值。"

图2

174份重组自交系苗期耐盐鉴定结果分布 A: 正常处理与盐胁迫下, 群体苗期株高分布小提琴图; B: 群体在盐胁迫下枯叶率分布直方图; C: 苗期耐盐等级评价结果; ***代表P < 0.001水平差异显著, 图基检验计算P值。"

表1

生殖生长期耐盐性表型统计结果"

处理
Treatment		 性状
Trait		 亲本Parentage 重组自交系Recombinant inbred lines
赤禾Chihe Lemont 均值
Mean		 标准差
SD		 变异系数CV 最小值Min. 最大值Max.
正常对照
Control		 株高 PH (cm) 224.5 96.8 130.04* 25.28 0.19 83.33 185.00
有效穗 TN 7.6 9.3 10.41 2.44 0.23 6.33 18.00
穗长 SL (cm) 29.5 16.8 26.25 2.93 0.11 18.83 35.67
实粒数 FGN 170.6 115.8 124.32** 127.58 0.34 141.00 801.00
结实率 SSR (%) 89.4 82.5 74.12** 10.96 0.15 44.95 97.95
单株产量 PY (g) 14.7 12.8 7.90** 2.57 0.33 3.10 17.06
千粒重 TGW (g) 23.1 26.8 21.55 3.17 0.15 15.27 30.88
盐胁迫
Salt treatment		 株高 PH (cm) 194.6 86.2 123.12 27.94 0.23 67.40 194.60
有效穗 TN 6.4 7.8 10.45 2.44 0.23 5.80 18.40
穗长 SL (cm) 25.7 15.5 26.15 2.91 0.11 17.93 33.27
实粒数 FGN 113.5 53.2 110.70 93.86 0.28 138.00 587.00
结实率 SSR (%) 59.5 37.9 0.69 0.13 0.19 34.46 92.26
单株产量 PY (g) 11.2 3.0 6.87 2.00 0.29 2.45 12.38
千粒重 TGW (g) 19.8 23.2 20.99 3.62 0.17 13.04 38.90

图3

174份重组自交系生殖生长期耐盐鉴定结果分布 A: 正常处理与盐胁迫下, 群体单株产量分布小提琴图; B: 群体耐盐系数分布直方图; C: 耐盐等级评价结果; ***代表P < 0.001水平差异显著。"

图4

142个SSR标记遗传连锁图和耐盐性QTL位置"

表2

3个水稻生育期耐盐性鉴定的关联QTL"

生长期
Growth period		 QTL 分子标记
Marker interval		 位置
Position (cM)		 LOD值
LOD value		 加性效应Additive effect 显性效应Dominant effect 贡献率
R2
芽期
Germination stage		 qGR-7.1 RM11-RM10 55.17 10.32 1.037 1.914 13.68
qGR-7.2 RM10-RM3826 69.77 7.88 0.923 2.060 11.33
qGR-7.3 RM3286-RM505 73.91 2.55 -0.429 2.134 1.32
qGR-10 RM8201-RM596 38.16 3.29 -0.659 1.206 2.40
苗期
Seedling stage		 qSDI-1 RM259-RM572 45.84 3.34 -0.224 -1.206 0.98
qSDI-2 RM525-RM1092 119.43 2.60 0.429 0.575 3.48
qSDI-4 RM335-RM5687 23.30 2.59 0.533 -2.083 5.98
qSDI-7.1 RM125-RM214 30.51 2.96 -0.515 -0.400 5.22
qSDI-7.2 RM8263-RM11 34.50 4.31 -0.683 -0.029 9.65
qSDI-10 RM6128-RM6824 46.81 2.62 0.460 -1.561 4.02
生殖生长期
Reproductive stage		 qPH-2 RM3355-RM263 88.53 3.77 0.006 0.174 0.77
qPH-8 RM223-RM210 76.28 3.18 -0.005 0.072 0.59
qPH-9 RM215-RM205 64.98 3.20 -0.010 0.206 2.64
qTN-4 RM5687-RM273 58.40 2.64 -0.029 0.346 4.53
qTN-5.1 RM169-RM289 29.75 3.17 0.006 -0.123 0.20
qTN-5.2 RM274-RM1054 88.17 2.67 -0.044 0.020 8.83
qFGN-5 RM405-RM413 5.92 3.11 0.006 0.191 0.31
qFGN-7 RM505-RM3589 76.91 2.52 0.019 0.063 3.45
qSSR-5 RM413-RM5994 9.92 3.04 -0.005 0.274 0.12
qSSR-10 RM216-RM311 12.17 2.58 -0.031 0.122 5.78
qPY-2 RM5764-RM7581 9.65 2.54 0.029 0.040 4.48
qPY-3 RM16-RM227 108.01 2.68 -0.035 -0.013 6.24
qPY-5 RM413-RM5994 11.92 2.83 -0.012 0.306 0.74
qPY-9 RM201-RM215 57.47 2.61 -0.038 -0.018 8.02
qTGW-2.1 RM5764-RM7581 1.00 3.20 0.021 -0.002 8.01
qTGW-2.2 RM3355-RM263 90.53 2.57 0.001 0.280 0.06
qTGW-3.1 RM545-RM517 5.01 4.78 -0.004 0.219 0.35
qTGW-3.2 RM517-OSR13 11.11 4.14 -0.005 0.281 0.46
qTGW-4 RM5687-RM273 48.40 4.10 0.003 0.280 0.23
qTGW-6 RM3827-RM5371 77.46 2.80 0.004 0.188 0.31
qTGW-12.1 RM19-RM453 20.60 3.76 -0.015 0.088 4.13
qTGW-12.2 RM453-RM7003 27.44 4.47 -0.012 0.143 2.67
qTGW-12.3 RM309-RM235 71.71 2.96 -0.003 0.208 0.15
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