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作物学报 ›› 2025, Vol. 51 ›› Issue (5): 1178-1188.doi: 10.3724/SP.J.1006.2025.44146
王亚雯1，戚正阳1，尤佳琦1，聂新辉2，曹娟3，杨细燕1，涂礼莉1，张献龙1，王茂军1,2,*
WANG Ya-Wen1,QI Zheng-Yang1,YOU Jia-Qi1,NIE Xin-Hui2,CAO Juan3,YANG Xi-Yan1,TU Li-Li1,ZHANG Xian-Long1,WANG Mao-Jun1,2,*
摘要：
棉花是最重要的天然纺织纤维来源，同时是重要的油料来源。功能位点基因芯片作为一种可以提高育种值评估准确性和育种效率的工具，在棉花中应用较少。本研究制备了一款棉花60K功能位点基因芯片。该芯片制备基于已获得的棉花不同品种的Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq)、Chromatin Immunoprecipitation sequencing (ChIP-seq)、High-throughput Chromosome Conformation Capture (Hi-C)等组学数据，相较棉花领域已有的基因芯片，包含了更多经过多维组学数据注释的功能遗传变异的位点，所携带的有效功能信息更多。本研究将该芯片应用于棉花群体的全基因组关联分析中，鉴定到40个与棉花纤维品质性状相关的显著SNP位点，其中与纤维伸长率(FE)相关的显著位点共25个，与马克隆值(FM)相关的显著位点共l5个，与纤维强度(FS)相关的显著位点共2个，与纤维长度(FL)相关的显著位点共4个，与纤维整齐度(FU)相关的显著位点共4个。本研究中棉花60K功能位点基因芯片可应用于棉花种质资源评价、遗传定位及全基因组选择育种等方面，助力棉花基因组育种。
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