小麦籽粒淀粉分支酶同工酶基因型与酶活性关系研究

作物学报 ›› 2007, Vol. 33 ›› Issue (11): 1850-1855.

小麦籽粒淀粉分支酶同工酶基因型与酶活性关系研究

赵法茂^1,2;毕建杰¹;李天骄¹;逄孝云¹;王宪泽^1,*

1 山东农业大学作物生物学国家重点实验室, 山东泰安271018; 2 泰山学院生物系, 山东泰安271021

收稿日期:2007-02-07 修回日期:1900-01-01 出版日期:2007-11-12 网络出版日期:2007-11-12
通讯作者: 王宪泽

Relationship between Isozyme Genotypes and Activities of Starch Branching Enzyme in Wheat Grain

ZHAO Fa-Mao¹²,BI Jian-Jie¹,LI Tian-Jiao¹,PANG Xiao-Yun¹,WANG Xian-Ze^1*

1 State Key Laboratory of Crop Biology, Shandong Agricultural University，Tai’an 271018, Shandong；2 Department of Biology, Taishan College, Tai’an 271021, Shandong, China

Received:2007-02-07 Revised:1900-01-01 Published:2007-11-12 Published online:2007-11-12
Contact: WANG Xian-Ze

摘要/Abstract

摘要：

测定了71个小麦品种的籽粒淀粉分支酶活性，并采用非变性聚丙烯酰胺凝胶电泳（Native-PAGE），鉴定了分支酶同工酶的基因型，以明确小麦籽粒淀粉分支酶（SBE）各同工型等位基因的分布特点和基因型组成，阐明分支酶活性与分支酶同工酶基因型的相关性。结果表明，分支酶有SBEⅠ和SBEⅡ两个基因位点，SBEⅡ位点未显现多态性，SBEⅠ位点存在多样性。在SBEⅠ位点鉴定出A、B、D_ⅰ和D_ⅱ共4个等位基因位点，各等位基因位点出现频率分别为9.9%、76.1%、95.8%和57.8%；共检测到9种基因型，其中基因型D_ⅰD_ⅱB、D_ⅰB和D_ⅰD_ⅱ有较高的分布频率，分别为45.1%、28.2%和8.5%。从单一位点分析，分支酶活性与SBEⅠ位点有显著的相关性，与SBEⅡ位点相关性不显著。由此可见，小麦籽粒胚乳淀粉分支酶同工酶不同基因位点和基因型对酶活性具有不同的遗传效应。

关键词: 小麦, 淀粉分支酶, 等位基因, 基因型, 酶活性

Abstract:

To elucidate distribution of alleles, genotype composition and correlation between isozyme genotypes and enzyme activities of starch branching enzyme (SBE) in wheat (Triticum aestivum L.) grains, total activity of SBE was measured in seventy-one wheat cultivars with different provenances, including some typical hexaploid wheat from various province of China and some high-yielding modern varieties as well as eight exotic ones. The allele loci and genotypes of SBE isozymes were also investigated using native polyacrylamide gel electrophoresis (Native-PAGE). The results showed that there were two loci of starch branching enzyme, SBEⅠ and SBEⅡ. SBEⅡ failed to exhibit genetic polymorphism but SBEⅠmanifested genetic diversity. Four alleles were identified at SBEⅠ locus, that was A, B, D_ⅰ, and D_ⅱ, with frequency 9.9%, 76.1%, 95.8%, and 57.8%, respectively. Nine genotypes were determined, of them genotype D_ⅰD_ⅱB, D_ⅰB, and D_ⅰDⅱ took on high frequency with 45.1%, 28.2%, and 8.5%, respectively. From single locus analysis, starch branching enzyme activity correlated to SBEⅠ locus, but no significant correlation to SBEⅡ was observed. Therefore it indicated that different gene loci and genotypes of starch branching enzyme in wheat had different genetic impacts on enzyme activities.

Key words: Wheat (Triticum aestivum L), Starch branching enzyme, Allele, Genotype, Enzyme activity

赵法茂;毕建杰;李天骄;逄孝云;王宪泽. 小麦籽粒淀粉分支酶同工酶基因型与酶活性关系研究[J]. 作物学报, 2007, 33(11): 1850-1855.

ZHAO Fa-Mao;BI Jian-Jie;LI Tian-Jiao;PANG Xiao-Yun;WANG Xian-Ze. Relationship between Isozyme Genotypes and Activities of Starch Branching Enzyme in Wheat Grain[J]. Acta Agron Sin, 2007, 33(11): 1850-1855.

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