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作物学报 ›› 2009, Vol. 35 ›› Issue (4): 597-601.doi: 10.3724/SP.J.1006.2009.00597

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻抗条纹叶枯病基因Stv-bi的分子标记辅助选择

陈峰1，周继华23，张士永1，严长杰2，朱文银1，孙亚伟2，袁守江1，杨连群1*   

  1. 1山东省水稻研究所，山东济宁272177；2扬州大学教育部植物基因组学重点实验室，江苏扬州225009；3上海农业科学院作物育种栽培研究所，上海201106
  • 收稿日期:2008-08-11 修回日期:2008-12-08 出版日期:2009-04-12 网络出版日期:2009-02-13
  • 通讯作者: 杨连群
  • 基金资助:

    本研究由山东省农业科学院高新技术自主创新基金（2006YCX009和2007YCX007），江苏省高校重点实验室开放课题（K06001），山东省农业良种工程项目（2007LZ006-02），国家科技支撑计划项目（2006BAD01A01-5）资助。

Marker-assisted Selection for Stv-bi Gene Controlling Resistance to Rice Stripe Disease

CHEN Feng1,ZHOU Ji-Hua23,ZHANG Shi-Yong1,YAN Chang-Jie2,ZHU Wen-Yin1,SUN Ya-Wei2,YUAN Shou-Jiang1,YANG Lian-Qun1*   

  1. 1Shandong Rice Research Institute,Jining 272177,China;2Key Laboratory for Plant Functional Genomics,Ministry of education/Agricultural College,Yangzhou University,Yangzhou 225009,China;3Crop Breeding and Cultivation Reserch Institute, Shanghai Academy of Agricultural Sciences,Shanghai 201106,China
  • Received:2008-08-11 Revised:2008-12-08 Published:2009-04-12 Published online:2009-02-13
  • Contact: YANG Lian-Qun

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2283

被引次数

26

摘要：

水稻条纹叶枯病是我国黄淮及长江流域粳稻区重要的病害。由于水稻条纹叶枯病的发病受外界条件影响较大，人工接种抗性鉴定比较困难，利用与抗病基因紧密连锁的分子标记进行标记辅助选择对提高抗性育种效率具有重要意义。来自籼稻抗源Modan的Stv-bi是水稻育种中广泛应用的条纹叶枯病抗性基因。本研究设计了与Stv-bi紧密连锁的SSR及STS分子标记，用3个抗条纹叶枯病混合群体F30718(圣稻13/镇稻88)、F50701(武优34/T022//圣稻806)、F60702 (V6/T022//镇稻88)进行分子标记检测和田间条纹叶枯病抗性鉴定，其结果的符合率分别为99.3%、87.7%和91.8%。表明这些分子标记可以用于条纹叶枯病抗性基因Stv-bi分子标记辅助选择。

关键词: 水稻, 条纹叶枯病, 分子标记, 育种

Abstract:

Rice stripe disease is one of the most serious viral diseases in Huang-Huai and Yangtze River japonica cultivating area in China, and has caused severe loss in rice production. The pathogen is rice stripe virus (RSV) and transmitted by the small brown plant-hopper, Laodelphax striatellus Fallen. Moreover, rice stripe disease is difficult to assess by the way of artificial inoculation, and easily affected by natural conditions, thereby marker-assisted selection using molecular markers closely linked to disease-resistant gene to improve the efficiency of resistance breeding programs is of great significance. At present, Stv-bi is a widely utilized resistant gene for stripe virus disease in rice breeding that came from indica variety Modan, and Stv-bi has been fine mapped on chromosome 11. In this study, eight molecular markers, including three SSR (Simple Sequence Repeat) and five STS (Sequence-tagged Sites), closely linked to Stv-bi, were developed and displayed polymorphic between Shengdao 13 and Zhendao 88. Among them, three markers, H21, H11-8 and H11-12 were subsequently used for marker-assisted selection. The individual seedlings of three compound breeding populations, F30718 (Shengdao 13/Zhendao 88), F50701 (Wuyou 34/T022//Shengdao 806), and F60702 (V6/T022//Zhendao 88) were genotyped with molecular markers H21, H11-8 and H11-12. The lines from these populations checked by marker-assisted selection to rice strip disease were also investigated under field conditions at next generation. The consistency between field performance and the marker genotype in the three compound breeding populations was 99.3%, 87.7%, and 91.8%, respectively. The results indicated that these molecular markers can be applied for marker-assisted selection in the improvement of resistance to RSV.

Key words: Rice, Stripe virus disease, Molecular marker, Breeding

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