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作物学报 ›› 2009, Vol. 35 ›› Issue (5): 816-820.doi: 10.3724/SP.J.1006.2009.00816

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小豆SSR引物在绿豆基因组中的通用性分析

王丽侠，程须珍，王素华，刘长友，梁辉   

  1. 中国农业科学院作物科学研究所资源中心，北京100081
  • 收稿日期:2008-08-08 修回日期:2008-12-15 出版日期:2009-05-12 网络出版日期:2009-02-13
  • 通讯作者: 程须珍
  • 基金资助:

    本研究由农业部行业专项“食用豆类资源初级核心样本和新品种配套栽培技术研究与集成示范”（nyhyzx07-017,3-32)；中央级科研院所社会公益研究专项（082060302-14），国家自然科学基金（30871565）项目资助。

Transferability of SSR from Adzuki Bean to Mungbean

WANG Li-Xie,CHENG Xu-Zhen*,WANG Su-Hua,LIU Chang-You,LIANG Hui   

  1. Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-08-08 Revised:2008-12-15 Published:2009-05-12 Published online:2009-02-13
  • Contact: CHENG Xu-Zhen

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1760

被引次数

52

摘要：

分析了187对小豆SSR引物在绿豆基因组中的可转移性, 以期为绿豆分子遗传育种研究提供分析工具。结果表明，约75%的小豆SSR引物可在绿豆中有效扩增，但不同小豆连锁群SSR引物的可转移率存在差异。多态性分析发现80对引物中有29对在60份绿豆种质中可以检测到多态性, 等位变异数从2~7不等，平均为2.9, PIC指数从0.02~0.69，平均为0.36。UPGMA聚类及主坐标分析表明, 尽管同一省份的种质不能紧密聚在一起，但大多在聚类图上成簇状分布，说明相同来源的绿豆种质具有相似的遗传背景；此外，国外及我国边远地区的绿豆种质在遗传背景上与内部省份间存在明显差异。这些多态性SSR不仅可以有效用于绿豆分子遗传学研究，还可以用于不同来源绿豆种质资源的辅助鉴别。

关键词: 小豆, 绿豆, SSR, 可转移性

Abstract:

SSR (Simple sequence repeat) has been a popular tool in genetic studies. However, only a few SSR markers are available in mungbean (Vigna radiata L.). Adzuki bean (Vigna angularis L.) is a close relative of mungbean. In the study reported in this paper, we tested the transferability of adzuki bean SSR markers into mungbean. A total of 187 sets of SSR primers were tested and about 75% of them generated repeatable and clear products in mungbean, indicating a high homology between these two genomes. Of the transferable SSRs, 28 sets of primers generated polymorphic products among 60 mungbean genotypes with a total of 81 alleles detected. The average PIC (polymorphic information content) value is 0.36. Based on the SSR fingerprints, the 60 accessions can be divided into different groups agreeing well with their geographical origins. The exotic accessions were well separated from the local genotypes, indicating the former could be important sources for broadening the mungbean gene pool. The present study identified useful SSR markers for mungbean research, which will undoubtedly accelerate the genetic study of this crop.

Key words: Adzuki bean, Mungbean, SSR, Transferability

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