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作物学报

作物学报 ›› 2009, Vol. 35 ›› Issue (5): 958-961.doi: 10.3724/SP.J.1006.2009.00958

• 研究简报 • 上一篇    下一篇

玉米简单重复序列不对等交换的热点区域定位

汤继华12，马西青1，滕文涛1，严建兵1，戴景瑞1，李建生1*   

  1. 1中国农业大学国家玉米改良中心，北京100193；2河南农业大学农学院，河南郑州450002
  • 收稿日期:2008-09-19 修回日期:2008-12-15 出版日期:2009-05-12 网络出版日期:2009-02-16
  • 通讯作者: 李建生
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）重大专项（2006AA100103）资助。

Mapping Unequal Crossing Over Hotspot Region of Simple sequence Repeat in Maize

TANG Ji-Hua12,MA Xi-Qing1,TENG Wen-Tao1,YAN Jian-Bing1,DAI Jing-Rui1,LI Jian-Sheng1*   

  1. 1National Maize Improvement Center of China,China Agricultural University,Beijing 100193,China;2College of Agronmy.Hernan Agricultural University,Zhengzhou 450002,China
  • Received:2008-09-19 Revised:2008-12-15 Published:2009-05-12 Published online:2009-02-16
  • Contact: LI Jian-Sheng

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1315

被引次数

3

摘要：

生物基因组中简单重复序列的多态性是同源染色体不对等交换的结果之一，因此明确不对等交换的热点区域具有重要的理论意义。利用来源于优良玉米杂交种豫玉22的一套重组近交系(RIL)群体，对其遗传组成进行了SSR标记分析，发现40个不对等交换的SSR标记，不对等交换在群体间发生的概率介于0.34%~14.63%之间，每世代发生的频率为10-2~10-1，其中(AG)n重复的标记占发生不对等交换总标记的58.3%。有31个不对等交换标记分布于染色体上的11个热点区域，位于第9染色体以外的其它染色体上，其中第3和第5染色体上各分布2个不对等交换的热点区域。

关键词: 玉米, 简单重复序列（SSR）, 不对等交换, 热点区域

Abstract:

The polymorphism of simple sequence repeat (SSR) in biological genome is one result of unequal crossing over for homologous chromosomes; therefore it has theoretical importance in clarifying the hotspot regions of unequal crossing over. A set of recombinant inbred lines (RILs) population that derived form an elite hybrid Yuyu 22 was used in this study, its genetic components of the population was analyzed by means of SSR analysis, and 40 unequal crossing over SSR markers were found. The frequency of the unequal crossing over in the RIL population was 0.34–14.63%, with 10-2–10-1 frequency per generation, and the (AG)n repeat SSR markers accounted for 58.3%. There were 31 unequal crossing over markers locating on 11 chromosomal hotspot regions, distributing on 10 chromosomes except for chromosome 9, including two unequal crossing over hotspot regions each in chromosomes 3 and 5.

Key words: Maize, Simple sequence repeat(SSR), Unequal crossing over, Hotspot region

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