欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (06): 887-894.doi: 10.3724/SP.J.1006.2010.00887

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

水稻密穗突变体A989突变基因克隆和转基因植株分析

黎凌1,3,时振英1,沈革志2,王新其2,安林升1,张景六1,*   

  1. 1中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室，上海200032；2上海农业科学院作物研究所，上海201106；3上海交通大学农业与生物学院，上海200240
  • 收稿日期:2010-01-08 修回日期:2010-03-04 出版日期:2010-06-12 网络出版日期:2010-04-20
  • 通讯作者: 张景六,E-mail:jlzhang@sippe.ac.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA10A102）资助。

Dense-Panicle-Related Gene Cloning from Rice Mutant A989 and Transgenic Plant Analysis

LI Ling1,3,SHI Zhen-Ying1,CHEN Ge-Zhi2,WANG Xin-Qi2,AN Lin-Sheng1,ZHANG Jing-Liu1*   

  1. 1National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 2The Plant Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3 School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2010-01-08 Revised:2010-03-04 Published:2010-06-12 Published online:2010-04-20
  • Contact: ZHANG Jing-Liu,E-mail:jlzhang@sippe.ac.cn

PDF

2482

被引次数

2

摘要：

从水稻T-DNA插入突变群体中分离得到一个密穗突变体A989，表现晚开花、穗二级枝梗和小花数增加以及包颈。分子检测证明A989中T-DNA是单拷贝插入，通过inverse PCR的方法分离得到A989中T-DNA的旁邻序列，表明T-DNA插入在RCN2基因polyA加A位点后330 bp处；RT-PCR检测发现在A989中，RCN2基因的表达被显著上调。利用2×35S启动子在野生型水稻Zhonghua11中超表达RCN2基因，转基因植株表现为不抽穗，通过细胞学观察发现转基因植株能完成营养生长向生殖生长的转变，但是生殖生长期的分生组织在分化出二级枝梗原基后停止分化和生长。此外，通过比较部分开花相关基因在野生型Zhonghua11、突变体A989中的表达，推测了RCN2基因可能的作用途径。

关键词: 水稻, T-DNA, 密穗, RCN2基因

Abstract:

Rice (Oryza sativa L.) is a model monocotyledonous plant for genetic study due to its small genome size. Along with the completion of genome sequencing, gene cloning and function study becomes the most important task. The concomitant methodology of reverse genetics has played fundamental roles in identifying and studying rice genes in recent years. Rice heading time and inflorescence architecture are two inter-relating and agriculturally important characters. In Arabidopsis, TFL1 gene takes part in the configuration process and transition of growth stage, including flowering. In rice, there are four TFL1 gene homologies, RCN1-4, over-expression of any one of which could result in delayed flowering and abnormal inflorescence architecture. In this study, a mutant A989 with the character of dense panicle and late flowering was isolated from our T-DNA insertion population. Genetic and molecular analysis proved that in mutant A989, insertion of T-DNA nearby the RCN2 gene caused its over-expression, and resulted in the phenotype of dense panicle and late flowering. We further made RCN2 gene over-expressed driven by double 35S promoter, and analyzed characters of the transformants. Possible pathway of RCN2 gene function was discussed.

Key words: Rice, T-DNA, Dense panicle, RCN2

[1] Wang J(王江), Li L(李琳), Wan X-S(宛新杉), An L-S(安林升), Zhang J-L(张景六), Hong M-M(洪孟民). Generation and molecular analysis of a population of transgenic rice plants carrying Ds element. J Plant Physiol (植物生理学报), 2000, 26(6): 501-506 (in Chinese with English abstract)

[2] Lee SJung K H, An G-765, Chung Y Y. Isolation and characterization of a rice cysteine protease gene, OsCP1, using T-DNA gene-trap system. Plant Mol Biol, 2004, 54: 755,

[3] Oikawa T-700, Koshioka M, Kojima K, Yoshida H, Kawata M. A role of OsGA20ox1, encoding an isoform of gibberellin 20-oxidase, for regulation of plant stature in rice. Plant Mol Biol, 2004, 55: 687

[4] Peng L T-885, Shi Z Y, Li L, Shen G Z, Zhang J L. Overexpression of transcription factor OsLFL1 delays flowering time in Oryza sativa. J Plant Physiol, 2008, 165: 876

[5] Shannon S-892, Meeks-Wagner D R. A mutation in the Arabidopsis TFL1 gene affects inflorescence meristem development. Plant Cell, 1991, 3: 877

[6] Nakagawa M, Shimamoto K, Kyozuka J. Overexpression of RCN1 and RCN2, rice TERMINAL FLOWER 1/CENTRORADIALIS homologs, confers delay of phase transition and altered panicle morphology in rice. Plant J, 2002, 29: 743-750

[7] Zhang S, Hu W, Wang L, Lin C, Cong B, Sun C, Luo D. TFL1/CEN-like genes control intercalary meristem activity and phase transition in rice. Plant Sci, 2005, 168: 1393-1408

[8] Wang J, Li L, Wan X, An L, Zhang J. Distribution of T-DNA carrying a Ds element on rice chromosomes. Sci China (Ser C), 2004, 47: 322-331

[9] Kyozuka J, Kobayashi T, Morita M, Shimamoto K. Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol, 2000, 41: 710-718

[10] Kyozuka J, Konishi S, Nemoto K, Izawa T, Shimamoto K. Down-regulation of RFL, the FLO/LFY homolog of rice, accompanied with panicle branch initiation. Proc Natl Acad Sci USA, 1998, 95: 1979-1982

[11] Tadege M, Sheldon C C, Helliwell C A, Upadhyaya N M, Dennis E S, Peacock W J. Reciprocal control of flowering time by OsSOC1 in transgenic Arabidopsis and by FLC in transgenic rice. Plant Biotechnol J, 2003, 1: 361-369

[12] Aubert D, Chen L, Moon Y H, Martin D, Castle L A, Yang C H, Sung Z R. EMF1, a novel protein involved in the control of shoot architecture and flowering in Arabidopsis. Plant Cell, 2001, 13: 1865-1875

[13] Chardon F, Damerval C. Phylogenomic analysis of the PEBP gene family in cereals. J Mol Evol, 2005, 61: 579-590

[14] Yoo S Y, Kardailsky I, Lee J S, Weigel D, Ahn J H. Acceleration of flowering by overexpression of MFT (MOTHER OF FT AND TFL1). Mol Cells, 2004, 17: 95-101

[15] Kardailsky I, Shukla V K, Ahn J H, Dagenais N, Christensen S K, Nguyen J T, Chory J, Harrison M J, Weigel D. Activation tagging of the floral inducer FT. Science, 1999, 286: 1962-1965

[16] Kobayashi Y, Kaya H, Goto K, Iwabuchi M, Araki T. A pair of related genes with antagonistic roles in mediating flowering signals. Science, 1999, 286: 1960-1962

[17] An H, Roussot C, Suarez-Lopez P, Corbesier L, Vincent C, Pineiro M, Hepworth S, Mouradov A, Justin S, Turnbull C, Coupland G. CONSTANS acts in the phloem to regulate a systemic signal that induces photoperiodic flowering of Arabidopsis. Development, 2004, 131: 3615-3626

[18] Ayre B G, Turgeon R. Graft transmission of a floral stimulant derived from CONSTANS. Plant Physiol, 2004, 135: 2271-2278

[19] Komiya R, Ikegami A, Tamaki S, Yokoi S, Shimamoto K. Hd3a and RFT1 are essential for flowering in rice. Development, 2008, 135: 767-774

[20] Mathieu J, Warthmann N, Ku¨ttner F, Schmid M. Export of FT protein from phloem companion cells is sufficient for floral induction in Arabidopsis. Curr Biol, 2007, 17: 1055-1060

[21] Liljegren S J, Gustafson-Brown C, Pinyopich A, Ditta G S, Yanofsky M F. Interactions among APETALA1, LEAFY, and TERMINAL FLOWER1 specify Meristem Fate. Plant Cell,1999, 11: 1007-1018

[22] Komatsu M, Maekawa M, Shimamoto K, Kyozuka J. The LAX1 and FRIZZY PANICLE 2 genes determine the inflorescence architecture of rice by controlling rachis-branch and spikelet development. Dev Biol, 2001, 231: 364-373
[1] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[2] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[3] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[4] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[5] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[6] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[7] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[8] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
[9] 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099.
[10] 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
[11] 雷松翰, 范骏扬, 车艳奕, 代永东, 郑雨萌, 田维江, 桑贤春, 王晓雯. 水稻内卷叶突变体acl3的鉴定及调控基因的功能分析[J]. 作物学报, 2025, 51(6): 1467-1479.
[12] 李福媛, 杨奕, 马继琼, 许明辉, 林良斌, 孙一丁. 水稻OsPUB4基因克隆、激素诱导表达分析与互作蛋白筛选[J]. 作物学报, 2025, 51(6): 1690-1700.
[13] 王梦宁, 谢可冉, 高逖, 王飞, 任孝俭, 熊栋梁, 黄见良, 彭少兵, 崔克辉. 水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系[J]. 作物学报, 2025, 51(5): 1347-1362.
[14] 盛倩男, 方娅婷, 赵剑, 杜思垚, 胡行珍, 余秋华, 朱俊, 任涛, 鲁剑巍. 不同养分管理措施对稻田和旱地油菜产量的影响及其对冻害的响应[J]. 作物学报, 2025, 51(5): 1286-1298.
[15] 翁文安, 邢志鹏, 胡群, 魏海燕, 廖萍, 朱海滨, 瞿济伟, 李秀丽, 刘桂云, 高辉, 张洪程. 无人化旱直播水稻产量形成特征及其能量与经济效益研究[J]. 作物学报, 2025, 51(5): 1363-1377.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2