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作物学报 ›› 2010, Vol. 36 ›› Issue (06): 887-894.doi: 10.3724/SP.J.1006.2010.00887

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

水稻密穗突变体A989突变基因克隆和转基因植株分析

黎凌1,3,时振英1,沈革志2,王新其2,安林升1,张景六1,*   

  1. 1中国科学院上海生命科学研究院植物生理生态研究所植物分子遗传国家重点实验室，上海200032；2上海农业科学院作物研究所，上海201106；3上海交通大学农业与生物学院，上海200240
  • 收稿日期:2010-01-08 修回日期:2010-03-04 出版日期:2010-06-12 网络出版日期:2010-04-20
  • 通讯作者: 张景六,E-mail:jlzhang@sippe.ac.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2006AA10A102）资助。

Dense-Panicle-Related Gene Cloning from Rice Mutant A989 and Transgenic Plant Analysis

LI Ling1,3,SHI Zhen-Ying1,CHEN Ge-Zhi2,WANG Xin-Qi2,AN Lin-Sheng1,ZHANG Jing-Liu1*   

  1. 1National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 2The Plant Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; 3 School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
  • Received:2010-01-08 Revised:2010-03-04 Published:2010-06-12 Published online:2010-04-20
  • Contact: ZHANG Jing-Liu,E-mail:jlzhang@sippe.ac.cn

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被引次数

2

摘要：

从水稻T-DNA插入突变群体中分离得到一个密穗突变体A989，表现晚开花、穗二级枝梗和小花数增加以及包颈。分子检测证明A989中T-DNA是单拷贝插入，通过inverse PCR的方法分离得到A989中T-DNA的旁邻序列，表明T-DNA插入在RCN2基因polyA加A位点后330 bp处；RT-PCR检测发现在A989中，RCN2基因的表达被显著上调。利用2×35S启动子在野生型水稻Zhonghua11中超表达RCN2基因，转基因植株表现为不抽穗，通过细胞学观察发现转基因植株能完成营养生长向生殖生长的转变，但是生殖生长期的分生组织在分化出二级枝梗原基后停止分化和生长。此外，通过比较部分开花相关基因在野生型Zhonghua11、突变体A989中的表达，推测了RCN2基因可能的作用途径。

关键词: 水稻, T-DNA, 密穗, RCN2基因

Abstract:

Rice (Oryza sativa L.) is a model monocotyledonous plant for genetic study due to its small genome size. Along with the completion of genome sequencing, gene cloning and function study becomes the most important task. The concomitant methodology of reverse genetics has played fundamental roles in identifying and studying rice genes in recent years. Rice heading time and inflorescence architecture are two inter-relating and agriculturally important characters. In Arabidopsis, TFL1 gene takes part in the configuration process and transition of growth stage, including flowering. In rice, there are four TFL1 gene homologies, RCN1-4, over-expression of any one of which could result in delayed flowering and abnormal inflorescence architecture. In this study, a mutant A989 with the character of dense panicle and late flowering was isolated from our T-DNA insertion population. Genetic and molecular analysis proved that in mutant A989, insertion of T-DNA nearby the RCN2 gene caused its over-expression, and resulted in the phenotype of dense panicle and late flowering. We further made RCN2 gene over-expressed driven by double 35S promoter, and analyzed characters of the transformants. Possible pathway of RCN2 gene function was discussed.

Key words: Rice, T-DNA, Dense panicle, RCN2

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