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作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1409-1413.doi: 10.3724/SP.J.1006.2010.01409

• 研究简报 • 上一篇    下一篇

大豆7S球蛋白α-亚基缺失型种质创新

刘珊珊,滕卫丽**,张彬彬,葛玉君,刁桂珠,郑天慧,姜自芹,曾蕊,吴帅,李文滨*   

  1. 东北农业大学农学院 / 教育部大豆生物学重点实验室,黑龙江哈尔滨 150030
  • 收稿日期:2010-01-04 修回日期:2010-04-21 出版日期:2010-08-12 网络出版日期:2010-06-11
  • 通讯作者: 李文滨, E-mail: wenbinli@yahoo.com
  • 基金资助:

    本研究由哈尔滨市科技攻关计划项目(2008RFLXN002),东北农业大学引进人才项目(2005YJRC003),博士后研究人员落户黑龙江科研启动项目(2009HB009)和国家转基因生物亲品种培育科技重大专项子任务(2008ZX08004-004)资助。

Development of Soybean Germplasm Lacking of 7S Globulin α-Subunit

LIU Shan-Shan,TENG Wei-Li**,ZHANG Bin-Bin,GE Yu-Jun,DIAO Gui-Zhu,ZHENG Tian-Hui,JIANG Zi-Qin,ZENG Rui,WU Shuai,LI Wen-Bin*   

  1. Soybean Research Institute,Northeast Agricultural University/Key Laboratory of Soybean Biology,Ministry of Education,Harbin 150030,China
  • Received:2010-01-04 Revised:2010-04-21 Published:2010-08-12 Published online:2010-06-11
  • Contact: LI Wen-Bin, E-mail: wenbinli@yahoo.com

摘要:

采用常规杂交育种方法,人工去雄、授粉,以10份综合农艺性状优良的黑龙江省主栽品种(或优良品系)为母本、亚基组成为(α'+α+11S酸性亚基)-缺失型育种材料日B为父本进行有性杂交。将F1杂交种南繁,单粒点播、单株收获得到F2代分离群体。聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析表明,F1代杂交种子7S球蛋白α'-与α-亚基的表现型全部为正常型。在杂交F2代种子中获得了具有中国大豆遗传背景的α-缺失、(α+A1aA1bA2)-缺失、A3-缺失、(α'+A4)-缺失和(α '+α)-缺失的贮藏蛋白亚基组成新类型种质, 为我国大豆蛋白组分育种选择提供了重要的中间材料。

关键词: 大豆, 7S球蛋白, α-亚基缺失型, 种质创新

Abstract:

α-subunit of soybean β-conglycinin is one of the major allergens in soybean products. It is possible to reduce or remove the allergens from soybean by development of mutant lines. In this study, a cross was made between 10 major varieties in Heilongjiang province of China and “riB” lacking α'-, α-, and 11S acidic subunits in order to breed soybean variety characterized by α-subunit null. F1 seeds were sown individually and individual plants were harvested to obtain F2-progeny seeds. Subunit compositions of seeds in F1 and F2 were examined for storage protein by SDS-PAGE. The results showed that all the F1 seeds obtained from cross contained α'- and α-subunits. Five band types of 7S globulin in the F2-progeny were observed including α-null, (α+A1aA1bA2)-null, A3-null, (α'+A4)-null, and (α'+α)-null types.

Key words: Soybean, 7S globulin, α-subunit mull type

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