作物学报 ›› 2010, Vol. 36 ›› Issue (08): 1409-1413.doi: 10.3724/SP.J.1006.2010.01409
刘珊珊,滕卫丽**,张彬彬,葛玉君,刁桂珠,郑天慧,姜自芹,曾蕊,吴帅,李文滨*
LIU Shan-Shan,TENG Wei-Li**,ZHANG Bin-Bin,GE Yu-Jun,DIAO Gui-Zhu,ZHENG Tian-Hui,JIANG Zi-Qin,ZENG Rui,WU Shuai,LI Wen-Bin*
摘要:
采用常规杂交育种方法,人工去雄、授粉,以10份综合农艺性状优良的黑龙江省主栽品种(或优良品系)为母本、亚基组成为(α'+α+11S酸性亚基)-缺失型育种材料日B为父本进行有性杂交。将F1杂交种南繁,单粒点播、单株收获得到F2代分离群体。聚丙烯酰胺凝胶电泳(SDS-PAGE)法分析表明,F1代杂交种子7S球蛋白α'-与α-亚基的表现型全部为正常型。在杂交F2代种子中获得了具有中国大豆遗传背景的α-缺失、(α+A1aA1bA2)-缺失、A3-缺失、(α'+A4)-缺失和(α '+α)-缺失的贮藏蛋白亚基组成新类型种质, 为我国大豆蛋白组分育种选择提供了重要的中间材料。
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