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作物学报 ›› 2010, Vol. 36 ›› Issue (10): 1752-1759.doi: 10.3724/SP.J.1006.2010.01752

• 耕作栽培·生理生化 • 上一篇    下一篇

转EdHP1(氢离子焦磷酸化酶）基因烟草促进钾离子吸收的生理机制

金维环1,2,董建辉2,陈明2,*,陈耀锋1,李连城2,徐兆师2,马有志2,*   

  1. 1 西北农林科技大学农学院，陕西杨凌 712100；2 国家农作物基因资源与基因改良重大科学工程 / 农业部作物遗传育种重点开放实验室，中国农业科学院作物科学研究所，北京 100081
  • 收稿日期:2010-03-10 修回日期:2010-05-23 出版日期:2010-10-12 网络出版日期:2010-05-23
  • 通讯作者: 陈明, E-mail: chenming@mail.caas.net.cn; 马有志, E-mail: mayouzhi@yahoo.com.cn
  • 基金资助:
    本研究由国家高技术研究发展计划(863计划)项目(2008AA10Z124和2006AA10A111)和国家自然科学基金项目(30700508)资助。

Physiological Mechanisms of Enhanced Uptake of Potassium Nutrition in EdHP1 (H+-pyrophosphatase) Transgenic Tobacco (Nicotiana tabacum)

JIN  Wei-Huan12,DONG  Jian-Hui2,CHEN  Ming2*,CHEN  Yao-Feng1,LI  Lian-Cheng2,XU  Zhao-Shi2,MA  You-Zhi2*   

  1. 1 College of Agronomy, Northwest A&F University, Yangling 712100, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Crop Genetics and Breeding of Ministry of Agriculture, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2010-03-10 Revised:2010-05-23 Published:2010-10-12 Published online:2010-05-23
  • Contact: CHEN Ming,E-mail:chenming@mail.caas.net.cn;MA You-Zhi,E-mail:mayouzhi@yahoo.com.cn

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摘要： 钾是植物生长必须的重要矿质元素，提高作物对钾的吸收是提高作物产量的重要途径。本研究以前期遗传转化披碱草EdHP1(H+-pyrophosphatase)基因的烟草为材料，在低钾处理条件下比较了转基因烟草和野生型烟草的生理特性。结果显示，在低钾条件下，野生型烟草体内K+积累量只相当于转基因烟草的62.9%。野生型烟草的总根长、总根表面积、总根体积明显低于转基因烟草(P<0.05)，分别为转基因烟草的72.9%、68.1%和60.6%。在不同直径根系(0.5~2.0、2.5~4.5、>4.5 mm 分别为细根、中等根和粗根)中，细根发育差异最大，野生型烟草为转基因烟草的72.9%。低钾条件下，转基因烟草根系活力是野生型烟草的1.2倍，野生型烟草根系焦磷酸化酶活力相当于转基因烟草的46.4%，根部游离IAA含量相当于转基因烟草的66.2%。通过非损伤方法测定根部 K+和 H+流速结果显示，在根系表面积相似情况下，低钾条件下野生型烟草外排钾速率显著高于转基因烟草，而氢离子外排速率显著低于转基因烟草。综上所述，发达的根系和较高的K+转运体活性综合作用显著提高了转基因烟草根部对K+的吸收。

关键词: 烟草, K+吸收, 氢离子焦磷酸化酶, 根系

Abstract: Potassium is necessary for plant growth and enhancing uptake of potassium is important to improve crop yield. In this study, previous transgenic tobacco transformed with Elymus dahuricus EdHP1 (H+-pyrophosphatase) was used to compare the physiological characteristics between transgenic tobacco and wild type tobacco. The results showed that under starvation of potassium, the content of K+ accumulated in wild-type tobacco was 62.9% of that in transgenic tobacco, and the total root length, total root surface area, and total root volume in wild type tobacco were observably lower than those in transgenic tobacco (P<0.05), with the decrease of 72.9%, 68.1%, and 60.6%, respectively. In addition, in different root systems of fine roots (diameter=0.5–2.0 mm), middle roots (diameter= 2.5–4.0 mm) and coarse roots (diameter>4.5 mm), fine roots were biggest different between transgenic and wild type tobacco which fine roots were 72.9% of those in transgenic tobacco. The activity of root system in transgenic tobacco was 1.2 times as high as that in wild type tobacco. The activity of H+-pyrophosphatase and the content of free IAA in wide-type tobacco were only 46.4% and 66.2% of those in transgenic tobacco respectively. The results of non-destructive ion flux measurement showed that under starvation of potassium the efflux of K+ from wild type tobacco was observably higher than that from transgenic tobacco, but inverse result was found efflux of H+ with similarly total root surface area of transgenic tobacco and wild type tobacco. Two physiological mechanisms containing well-developed root system and higher activity of K+ transporters enhanced uptake of potassium in EdHP1 transgenic tobacco plants.

Key words: Tobacco, Uptake of potassium, H+-pyrophosphatase, Root system

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