欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2010, Vol. 36 ›› Issue (3): 422-427.doi: 10.3724/SP.J.1006.2010.00422

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻抗白叶枯病基因Xa14的遗传定位

鲍思元1,2，谭明谱3，林兴华1,*   

  1. 1华中农业大学作物遗传改良国家重点实验室，湖北武汉 430070；2咸宁学院基础医学院，湖北咸宁 437100；3南京农业大学生命科学学院，江苏南京 210095
  • 收稿日期:2009-09-23 修回日期:2009-10-02 出版日期:2010-03-12 网络出版日期:2009-12-21
  • 通讯作者: 林兴华，E-mail: xinghualin@mail.hzau.edu.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2001AA222161）资助。

Genetic Mapping of a Bacterial Blight Resistance Gene Xa14 in Rice

BAO Si-Yuan1,2,TAN Ming-Pu3,LIN Xing-Hua1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 College of Basic Medical, Xianning University, Xianning 437100, China; 3 Life Science College, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-09-23 Revised:2009-10-02 Published:2010-03-12 Published online:2009-12-21
  • Contact: LIN Xing-Hua，E-mail: xinghualin@mail.hzau.edu.cn

PDF

2104

被引次数

13

摘要：

Xa14是一个高抗菲律宾白叶枯病生理小种5的显性基因，Taura等将它定位在水稻第4染色体长臂末端。本研究利用中国国家基因中心的水稻第4染色体测序结果，用SSR标记对Xa14进行遗传定位，为进一步用图位克隆法克隆该基因奠定基础。利用775株IRBB14/IR24 F2中的145株高感群体，将基因Xa14限定在SSR标记HZR970-8和HZR988-1之间的区间，与两个分子标记的距离各为0.34 cM，并找到了在该群体中与基因共分离的HZR645-4、HZR669-2、HZR669-5和HZR669-7四个SSR标记。利用763株IRBB14/珍珠矮F2中158株高感群体，将基因Xa14限定在分子标记HZR648-5与RM280之间的区段，找到了一个与基因紧密连锁的SSR标记HZR648-5，与基因的距离为1.90 cM。将两个F2群体的定位结果进行整合，表明Xa14位于分子标记HZR970-8和HZR988-1之间的3个BAC克隆上，并与这两个标记紧密连锁。

关键词: 水稻, 白叶枯病, 抗性基因, Xa14, SSR, 定位

Abstract:

Bacterial blight of rice, which is a vascular bundle disease caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide, and leads to the yield of rice reduced greatly. The dominant gene Xa14 is highly resistant to Philippine race 5 of bacterial blight. The gene was located at the distal end on the long arm of chromosome 4 by Taura et al. Near-isogenic line in the background of IR24 namely IRBB14 carrying the geneXa14has been developed at International Rice Research Institute(IRRI). In order to construct the high-resolution likage maps for the Xa14 region on chromosome 4 for finally clone by positional cloning, tow F2 populations were used to estimate linkage based on marker genotype and reaction to disease inoculation with Xanthomonas oryzae pv. oryzae. Using 145 highly susceptible individuals from a total of 775 plants of F2 population of the cross between IRBB14 and IR24, the gene Xa14 was located in a 0.68 cM region on the nearby end of chromosome 4, which was flanked by the molecular markers, HZR970-8 and HZR988-1, with the distance of 0.34 cM between the flanked markers and Xa14, respectively, and completely cosegregated with the SSR markers, HZR645-4, HZR669-2, HZR669-5, and HZR669-7 in this population. Using 158 highly susceptible individuals from a total of 763 plants of F2 population of the cross between IRBB14 and ZZA, the gene Xa14 locus was mapped to the interval between HZR648-5 and RM280, and was 1.90 cM away from HZR648-5 which was the closest marker flanking the Xa14 locus. Combining recombination frequencies for the two populations together, the gene Xa14 was mapped to 3 BAC clones spanned approximately 300 kb in length between SSR markers HZR970-8 and HZR988-1.

Key words: Rice, Bacterial blight, Resistance gene, xa14, SSR, Genetic mapping


[1]Mew T W. Current status and future prospects of research on bacterial blight of rice. Ann Rev Phytopathol, 1987, 25: 359–382


[2] Ogawa T. Methods and strategy for monitoring race distribution and identification of resistance genes to bacterial leaf blight (Xanthomonas campestris pv. oryzae) in rice. Jpn Agric Res Q, 1993, 27: 71–80


[3] Lee K S, Rasabandith S, Angeles E R, Khush G S. Inheritance of resistance to bacterial blight in 21 cultivars of rice. Phytopathology, 2003, 93: 147–152


[4] Chen Y(陈艳), Hu J(胡军), Qian W(钱韦), Tian Y-C(田颖川), Zhao W-M(赵文明), He C-Z(何朝族). Characterization and molecular marker screening of a rice bacteria-resistant gene Xa-min(t). Prog Nat Sci (自然科学进展), 2003, 13(9): 1001–1004 (in Chinese with English abstract)


[5] Tan G-X(谭光轩), Ren X(任翔), Wong Q-M(翁清妹), Shi Z-Y(时振英), Zhu L-L(祝莉莉), He G-C(何光存). Mapping of a new resistance gene to bacterial blight in rice line introgressed from Oryza officinalis. Acta Genet Sin (遗传学报), 2004, 31(7): 724–729 (in Chinese with English abstract)


[6]Zhang Q(章琦). Highlights in identification and application of resistance genes to bacterial blight. Chin J Rice Sci (中国水稻科学), 2005, 19(5): 453–459 (in Chinese with English abstract)


[7]Yoshimura S, Yamanouchi U, Katayose Y, Toki S, Wang Z X, Kono I, Kurata N, Yano M, Iwata N, Sasaki T. Expression of Xa1, a bacterial blight-resistance gene in rice, is induced by bacterial inoculation. Proc Natl Acad Sci USA, 1998, 95: 1663–1668


[8]Lyer A S, McCouch S R. The rice bacterial blight resistance gene xa5 encodes a novel form of disease resistance. Mol Plant-Microbe Interact, 2004, 17: 1348–1354


[9] Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Gardner J, Wang B, Holsten T, Zhai W X, Zhu L H, Fauquwt C, Ronald P. A receptor kinase-like protein encoded by the rice disease resistance gene Xa21. Science, 1995, 270: 1804–1806


[10]Sun X, Cao Y, Yang Z, Xu C, Li X, Wang S, Zhang Q. Xa26, a gene conferring resistance to Xanthomonas oryzae pv. oryzae in rice, encodes an LRR receptor kinase-like proein. Plant J, 2004, 37: 517–527


[11]Gu K, Yang B, Tian D, Wu L, Wang D, Sreekala C, Yang F, Chu Z, Wang G L, White F F, Yin Z R. Gene expression induced by a type-III effector triggers disease resistance in rice. Nature, 2005, 435: 1122–1125


[12] Taura S, Ogawa T, Tabien R E, Khush G S, Yoshimura A, Omura T. The specific reaction of Taichung Native 1 to Philippine races of bacterial blight and inheritance of resistance to race 5. Rice Genet Newsl, 1987, 4: 101–102


[13] Taura S, Ogawa T, Tabien R E, Khush G S, Yoshimura A, Omura T. Resistance gene of rice cultivar, Taichung Native to Philippines races of bacterial blight pathogens. Jpn J Breed, 1992, 42: 195–201


[14] Kinoshita T. Report of the committee on gene symbolization and linkage map. Rice Genet Newsl, 1993, 10: 1–5


[15] Tan Z-B(谭震波), Zhang Q(章琦), Zhu L-H(朱立煌), Wang C-L(王春莲). RFLP mapping of a rice bacterial blight resistance gene Xa14. Hereditas (遗传), 1998, 20(6): 30–33 (in Chinese with English abstract)


[16] Ogawa T, Yamamoto T, Khush G S, Mew T W. Breeding of near-isogenic lines of rice with single genes for resistance to bacterial blight pathogen (Xanthomonas campestris pv. oryzae). Jpn J Breed, 1991, 41: 523–529


[17] Wakimoto S. The detemination of the presence of Xanthomonas oryzae by the phage technique. Scientific Bulletin of Faculty of Agriculture, Kyushu University, 1954, 14: 485–493 (in Japanese with English abstract)


[18]Kauffman H E, Reddy A P K, Hsieh S P Y, Merca S D. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Dis Rep, 1973, 57: 537–541


[19] Temnykh S, DeClerck G, Lukashova A, Lipovich L, Cartinhour S, McCouch S. Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): frequency, length variation, transposon associations, and genetic marker potential. Genome Res, 2001, 11: 1441–1152


[20] Temnykh S,Park W D, Ayres N, Cartinhour S, Harek N, Lipovich L, Cho Y G, Ishii T, McCouch S R. Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.). Theor Appl Genet, 2000, 100: 697–712


[21] Allard R W. Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia, 1956, 24: 235-278


[22]Kosambi D D. The estmation of map distance from recombination values. Ann Eugen, 1994, 12: 172–175


[23]Liu R-H(刘仁虎), Meng J-L(孟金陵). MapDraw: A microsoft excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas(遗传), 2003, 25(3): 317–321 (in Chinese with English abstract)


[24]Sanchez A C, Ilag L L, Yang D, Brar D S, Ausubel F, Khush G S, Yano M, Sasaki T, Li Z, Huang N. Genetic and physical mapping of xa13, a recessive bacterial blight resistance gene in rice. Theor Appl Genet, 1999, 98: 1022–1028


[25] Blair M W, McCouch S R. Microsatellite and sequence-tagged site markers diagnostic for the rice bacterial leaf blight resistance gene xa-5. Theor Appl Genet, 1997, 95: 174–184
[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[6] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[7] 邓钊, 江南, 符辰建, 严天泽, 符星学, 胡小淳, 秦鹏, 刘珊珊, 王凯, 杨远柱. 隆两优与晶两优系列杂交稻的稻瘟病抗性基因分析[J]. 作物学报, 2022, 48(5): 1071-1080.
[8] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[9] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[10] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[11] 王小雷, 李炜星, 欧阳林娟, 徐杰, 陈小荣, 边建民, 胡丽芳, 彭小松, 贺晓鹏, 傅军如, 周大虎, 贺浩华, 孙晓棠, 朱昌兰. 基于染色体片段置换系群体检测水稻株型性状QTL[J]. 作物学报, 2022, 48(5): 1141-1151.
[12] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[13] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[14] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[15] 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2