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作物学报 ›› 2010, Vol. 36 ›› Issue (3): 422-427.doi: 10.3724/SP.J.1006.2010.00422

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻抗白叶枯病基因Xa14的遗传定位

鲍思元1,2，谭明谱3，林兴华1,*   

  1. 1华中农业大学作物遗传改良国家重点实验室，湖北武汉 430070；2咸宁学院基础医学院，湖北咸宁 437100；3南京农业大学生命科学学院，江苏南京 210095
  • 收稿日期:2009-09-23 修回日期:2009-10-02 出版日期:2010-03-12 网络出版日期:2009-12-21
  • 通讯作者: 林兴华，E-mail: xinghualin@mail.hzau.edu.cn
  • 基金资助:

    本研究由国家高技术研究发展计划（863计划）项目（2001AA222161）资助。

Genetic Mapping of a Bacterial Blight Resistance Gene Xa14 in Rice

BAO Si-Yuan1,2,TAN Ming-Pu3,LIN Xing-Hua1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 College of Basic Medical, Xianning University, Xianning 437100, China; 3 Life Science College, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2009-09-23 Revised:2009-10-02 Published:2010-03-12 Published online:2009-12-21
  • Contact: LIN Xing-Hua，E-mail: xinghualin@mail.hzau.edu.cn

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被引次数

13

摘要：

Xa14是一个高抗菲律宾白叶枯病生理小种5的显性基因，Taura等将它定位在水稻第4染色体长臂末端。本研究利用中国国家基因中心的水稻第4染色体测序结果，用SSR标记对Xa14进行遗传定位，为进一步用图位克隆法克隆该基因奠定基础。利用775株IRBB14/IR24 F2中的145株高感群体，将基因Xa14限定在SSR标记HZR970-8和HZR988-1之间的区间，与两个分子标记的距离各为0.34 cM，并找到了在该群体中与基因共分离的HZR645-4、HZR669-2、HZR669-5和HZR669-7四个SSR标记。利用763株IRBB14/珍珠矮F2中158株高感群体，将基因Xa14限定在分子标记HZR648-5与RM280之间的区段，找到了一个与基因紧密连锁的SSR标记HZR648-5，与基因的距离为1.90 cM。将两个F2群体的定位结果进行整合，表明Xa14位于分子标记HZR970-8和HZR988-1之间的3个BAC克隆上，并与这两个标记紧密连锁。

关键词: 水稻, 白叶枯病, 抗性基因, Xa14, SSR, 定位

Abstract:

Bacterial blight of rice, which is a vascular bundle disease caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide, and leads to the yield of rice reduced greatly. The dominant gene Xa14 is highly resistant to Philippine race 5 of bacterial blight. The gene was located at the distal end on the long arm of chromosome 4 by Taura et al. Near-isogenic line in the background of IR24 namely IRBB14 carrying the geneXa14has been developed at International Rice Research Institute(IRRI). In order to construct the high-resolution likage maps for the Xa14 region on chromosome 4 for finally clone by positional cloning, tow F2 populations were used to estimate linkage based on marker genotype and reaction to disease inoculation with Xanthomonas oryzae pv. oryzae. Using 145 highly susceptible individuals from a total of 775 plants of F2 population of the cross between IRBB14 and IR24, the gene Xa14 was located in a 0.68 cM region on the nearby end of chromosome 4, which was flanked by the molecular markers, HZR970-8 and HZR988-1, with the distance of 0.34 cM between the flanked markers and Xa14, respectively, and completely cosegregated with the SSR markers, HZR645-4, HZR669-2, HZR669-5, and HZR669-7 in this population. Using 158 highly susceptible individuals from a total of 763 plants of F2 population of the cross between IRBB14 and ZZA, the gene Xa14 locus was mapped to the interval between HZR648-5 and RM280, and was 1.90 cM away from HZR648-5 which was the closest marker flanking the Xa14 locus. Combining recombination frequencies for the two populations together, the gene Xa14 was mapped to 3 BAC clones spanned approximately 300 kb in length between SSR markers HZR970-8 and HZR988-1.

Key words: Rice, Bacterial blight, Resistance gene, xa14, SSR, Genetic mapping


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