作物学报 ›› 2011, Vol. 37 ›› Issue (12): 2152-2157.doi: 10.3724/SP.J.1006.2011.02152
翟莹,雷婷婷,闫帆,黄开猛,李晓薇,张庆林,张海军,苏连泰,孙昕,王英,李景文*,王庆钰*
ZHAI Ying, LEI Ting-Ting,YAN Fan, HUANG Kai-Meng, LI Xiao-Wei, ZHANG Qing-Lin, ZHANG Hai-Jun, SU Lian-Tai, SUN Xin, WANG Ying, LI Jing-Wen*,WANG Qing-Yu*
摘要: 通过对大豆吉林32未成熟胚表达谱的分析,利用RT-PCR技术从大豆中克隆了一个新的脯氨酸富集蛋白基因,命名为GmPRP。GmPRP的开放阅读框长396 bp, 其分子量13.79 kD,具有131个氨基酸残基,等电点8.96,其DNA序列无内含子。GmPRP蛋白序列N端含有一段信号肽,中间为脯氨酸富集区,C端为半胱氨酸富集区。该蛋白与四季豆和木豆的PRP同源性最高, 具有较近的亲缘关系。GmPRP的683 bp启动子序列含有10种与逆境相关的顺式作用元件,分别为ABRE-like、G-box、W-box、GT-1、MYB、MYC、BIHD10s、DPBF、SEBF和WRKY。实时荧光定量PCR分析表明, 该基因表达量在大豆的根和叶中最高,在茎和胚中其次,在花中最低,且受干旱、高盐、低温、机械伤害及SA(水杨酸)、ETH(乙烯)、ABA(脱落酸)、MeJA (茉莉酸甲酯)的诱导上调表达。
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