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作物学报 ›› 2012, Vol. 38 ›› Issue (05): 840-847.doi: 10.3724/SP.J.1006.2012.00840

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

应用RSAP、SRAP和SSR分析苎麻种质亲缘关系

邹自征1,2，陈建华1,*，栾明宝1,*，郭劲霞2，王超3，王晓飞1，许英1，孙志民1   

  1. 1中国农业科学院麻类研究所 / 农业部茎纤维生物质与工程微生物重点开放实验室, 湖南长沙 410205；2益阳医学高等专科学校, 湖南益阳413000；3中南大学生物科学与技术学院，湖南长沙 410205
  • 收稿日期:2011-10-13 修回日期:2012-01-19 出版日期:2012-05-12 网络出版日期:2012-03-05
  • 通讯作者: 陈建华, E-mail: cjhbt@sina.com, Tel: 13908494958; 栾明宝, E-mail: luanmingbao2002@126.com, Tel:0731-88998518
  • 基金资助:

    本研究由国家自然科学基金项目(30900913), 国家科技支撑计划项目(2006BAD13B04-2)和中国农业科学院科技经费项目资助。

Evaluation of Genetic Relationship in Ramie Based on RSAP, SRAP, and SSR

ZOU Zi-Zheng1,2,CHEN Jian-Hua1,*,LUAN Ming-Bao1,*,GUO Jin-Xia2,WANG Chao3,WANG Xiao-Fei1,XU Ying1,SUN Zhi-Min1   

  1. 1 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences / Key Laboratory of Stem fiber Biomass and Engineering Microbilogy, Ministry of Agriculture, Changsha 420105, China; 2 Yiyang Medical School, Yiyang 413000, China; 3 School of Biological Science and Technology, Central South University, Changsha 410205, China
  • Received:2011-10-13 Revised:2012-01-19 Published:2012-05-12 Published online:2012-03-05
  • Contact: 陈建华, E-mail: cjhbt@sina.com, Tel: 13908494958; 栾明宝, E-mail: luanmingbao2002@126.com, Tel:0731-88998518

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被引次数

26

摘要： 分别采用RSAP、SRAP和SSR 3种分子标记和田间性状对16份苎麻种质进行亲缘关系聚类，结果表明, 每对引物扩增出的多态性位点，SRAP标记最多，RSAP次之，SSR较少；根据SRAP标记和RSAP标记分类的结果都与RSAP+SRAP+SSR联合分类的结果基本一致，相关达到了极显著水平，而与SSR标记分类结果差异较大；分子标记聚类结果与田间性状的聚类结果接近程度, 依次为SRAP+RSAP+SSR>SRAP>RSAP>>SSR。在检测苎麻种质亲缘关系中，SRAP标记效果最优，RSAP标记稍逊，SSR标记最差。RSAP标记能较好地显示苎麻种属间的多态性和亲缘关系。以RSAP、SRAP、SSR标记联合分析，能更好地揭示种质之间的亲缘关系。

关键词: 苎麻, RSAP, SRAP, SSR, 亲缘关系

Abstract: Ramie [Boehmeria nivea (L.) Gaud.] researches in molecular biology lags behind, there is a necessary to develop molecular markers in the genetic relationship analysis of ramie. In this paper, three molecular markers-RSAP, SRAP and SSR were used to cluster 16 Ramie germplasm. The result showed that each pair of primers could provide polymorphic sites and the site number was SRAP>RSAP>>SSR. Both results clustered based on SRAP and RSAP showed high consistency with that clustering based on combination of SRAP, RSAP and SSR, and the highly significant correlation coefficients were given. However, results of clustering based on SSR marker were quite different from the results based on combined markers. The rank order of similarities between the clustering results based on the molecular marker and phenotype was SRAP+RSAP+SSR>SRAP>RSAP>>SSR. For the study of genetic relationship among ramie germplasm, the efficiency of SRAP marker was slightly higher than that of RSAP marker, but that of SSR was low. Based on RSAP marker could efficiently display the polymorphism and genetic relationship among ramie varieties. Using the combined markers of RSAP&SRAP&SSR could be to reveal the genetic relationship of germplasm.

Key words: Ramie, RSAP, SRAP, SSR, Relationship

[1]Lu H-R(卢浩然). Cultivation Science of Chinese Fiber Crops (中国麻类作物栽培学). Beijing: China Agriculture Press, 1992

[2]Jie Y-C(揭雨成), Zhou Q-W(周青文), Chen P-D(陈佩度). Genetic relation analysis of ramie cultivars with RAPD mark. Acta Agron Sin (作物学报), 2002, 28(2): 254–259 (in Chinese with English abstract)

[3]Qiu C-S(邱财生), Cheng C-H(程超华), Zhao L-L(赵立宁), Li Y-J(李育君), Zang G-G(臧巩固). Genetic relationship among Boehmeria nivea spp. revealed by RAPD. Hubei Agric Sci (湖北农业科学), 2011, 50(7): 1499–1501 (in Chinese with English abstract)

[4]Jiang Y-B(蒋彦波), Jie Y-C(揭雨成), Zhou J-L(周建林), Xing H-C(邢虎成), She W(佘玮). Isolation and characterization of microsatellites from ramie [Boehmeria nivea L. Gaud.]. Acta Agron Sin (作物学报), 2007, 33(1): 158–162 (in Chinese with English abstract)

[5]Xing X-L(邢秀龙). Development of Microsatellite Markers from Ramie and Its Application for Preliminary Study of Heterosis Forecast. MS Dissertation of Huazhong Agricultural University, 2008 (in Chinese with English abstract)

[6]Chen J H, Luan M B, Song S F, Zou Z Z,Wang X F, Xu Y, Sun Z M. Isolation and characterization of EST-SSRs in the Ramie. Afr J Microbiol Res, 2011, 5: 3504–3508

[7]Zhao Z-W(赵中伟), Xu L-L(徐玲玲), Li T-J(李同建), Liao L(廖亮), Pan Q-H(潘其辉), Lai Z-J(赖占均), Shi Q-H(石庆华). Sampling strategy of Boehmeria nivea population by SSR molecular markers. J Anhui Agric Sci (安徽农业科学), 2009, 37(19): 8901–8903 (in Chinese with English abstract)

[8]Ding M-Z(丁明忠), Pan G-T(潘光堂), Zhang Z-H(张中华), Yang Y(杨燕), Li L-A(李立安). Genetic relation analysis of Boehmeria nivea cultivars or lines from Sichuan with ISSR and construction of the molecular markers linked to male sterile Geng. J Nucl Agric Sci (核农学报), 2008, 22(2): 183–187 (in Chinese with English abstract)

[9]Wen L(温岚), Yu C-M(喻春明), Wang Y-Z(王延周), Chen P(陈平), Chen J-K(陈继康), Tan L-T(谭龙涛), Xiong H-P(熊和平). Application of molecular marker SRAP on analysis of genetic diversity of polyembryonic ramie seedlings. J Hunan Agric Univ (Nat Sci) (湖南农业大学学报?自然科学版), 2011, 37(3): 243–247 (in Chinese with English abstract)

[10]Li G, Quiros C F. Sequence-related amplified polymorphisim (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet, 2001, 103: 455–461

[11]Fang X-J(方宣钧), Wu W-R(吴为人), Tang J-L(唐纪良). DNA Marker Assisted Selection in Crop Breeding (作物DNA标记辅助育种). Beijing: Science Press, 2001. pp 1–9 (in Chinese)

[12]Du X-H(杜晓华), Wang D-Y(王得元), Gong Z-H(巩振辉). Development and refinement of a new marker technique restriction site amplified polymorphism (RSAP). J Northwest A&F Univ (Nat Sci Edn)(西北农林科技大学学报?自然科学版), 2006, 34(9): 45–49 (in Chinese with English abstract)

[13]Du X-H(杜晓华), Wang D-Y(王得元), Gong Z-H(巩振辉). Estimation and comparison of genetic distances among elite inbred lines in hot pepper (Capsicum annuum L.) by RSAP and SSR. J Northwest A&F Univ (Nat Sci Edn)(西北农林科技大学学报?自然科学版), 2007, 35(7): 97–102 (in Chinese with English abstract)

[14]Qiao L-X(乔利仙), Weng M-L(翁曼丽), Kong F-N(孔凡娜), Dai J-X(戴继勋), Wang B(王斌). The application of RSAP marker technique in diversity detection and germplasm identification of porphyra. Period Ocean Univ China (Nat Sci)(中国海洋大学学报?自然科学版), 2007, 37(6): 951–956 (in Chinese with English abstract)

[15]Song G-L(宋国立), Cui R-X(崔荣霞), Wang K-B(王坤波), Guo L-P(郭立平), Li S-H(黎绍惠), Wang C-Y(王春英), Zhang X-D(张香娣). A rapid improved CTAB method for extraction of cotton genomic DNA. Acta Gossypii Sin (棉花学报), 1998, 10(5): 273–275 (in Chinese with English abstract)

[16]Zhang J(张军), Wu Y-T(武耀廷), Guo W-Z(郭旺珍), Zhang T-Z(张天真). Fast screening of microsatellite markers in cotton with page/silver staining. Acta Gossypii Sin (棉花学报), 2000, 12(5): 267–269 (in Chinese with English abstract)

[17]Luan M-B(栾明宝), Chen J-H(陈建华), Xu Y(许英), Wang X-F(王晓飞), Sun Z-M(孙志民). Method of establishing ramie core collection. Acta Agron Sin (作物学报), 2010, 36(12): 2099–2106 (in Chinese with English abstract)

[18]Liu L J, Peng D X, Wang B. Genetic relation analysis on ramie [Boehmeria nivea (L.) Gaud.] inbred lines by SRAP markers. Agric Sci China, 2008, 7(8): 944–949

[19]Du X Y, Zhang Q L, Luo Z R. Comparison of four molecular markers for genetic analysis in Diospyros L. (Ebenaceae). Plant Syst Evol, 2009, 281: 171–181

[20]Du X-H(杜晓华). Development of RSAP-a Novel DNA Marker Technique and Its Application in Pepper Genetics and Breeding. PhD Dissertation of Northwest A&F University, 2006 (in Chinese with English abstract)

[21]Rus-Kortekaas W, Smulders M J M, Arens P, Vosman B. Direct comparison of levels of genetic variation in tomato detected by GACA-containing microsatellite probe and by randomamplified polymorphic DNA. Genome, 1994, 37: 375–381

[22]Chen J-H(陈建华), Luan M-B(栾明宝), Xu Y(许英), Wang X-F(王晓飞), Sun Z-M(孙志民), Zou Z-Z(邹自征), Xiong H-P(熊和平). Construction of core germplasm in ramie. Plant Fiber Sci China (中国麻业科学), 2011, 33(2): 59–64 (in Chinese with English abstract)

[23]Zhou J-L(周建林), Jie Y-C(揭雨成), Jiang Y-B(蒋彦波), Hu X(胡翔), Zhang J(张健). Genetic relation analysis on ramie cultivars with microsatellite Markers. Acta Agron Sin (作物学报), 2004, 30(3): 289–292 (in Chinese with English abstract)

[24]Ye Y-M(叶要妹). Assessment of the germplasm genetic diversity of Zinnia elegans inbred lines detected by RAPDs and ISSRs. J Central South Univ For Technol (中南林业科技大学学报), 2008, 28(4): 36–41 (in Chinese with English abstract)
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