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作物学报 ›› 2012, Vol. 38 ›› Issue (05): 840-847.doi: 10.3724/SP.J.1006.2012.00840

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

应用RSAP、SRAP和SSR分析苎麻种质亲缘关系

邹自征1,2，陈建华1,*，栾明宝1,*，郭劲霞2，王超3，王晓飞1，许英1，孙志民1   

  1. 1中国农业科学院麻类研究所 / 农业部茎纤维生物质与工程微生物重点开放实验室, 湖南长沙 410205；2益阳医学高等专科学校, 湖南益阳413000；3中南大学生物科学与技术学院，湖南长沙 410205
  • 收稿日期:2011-10-13 修回日期:2012-01-19 出版日期:2012-05-12 网络出版日期:2012-03-05
  • 通讯作者: 陈建华, E-mail: cjhbt@sina.com, Tel: 13908494958; 栾明宝, E-mail: luanmingbao2002@126.com, Tel:0731-88998518
  • 基金资助:

    本研究由国家自然科学基金项目(30900913), 国家科技支撑计划项目(2006BAD13B04-2)和中国农业科学院科技经费项目资助。

Evaluation of Genetic Relationship in Ramie Based on RSAP, SRAP, and SSR

ZOU Zi-Zheng1,2,CHEN Jian-Hua1,*,LUAN Ming-Bao1,*,GUO Jin-Xia2,WANG Chao3,WANG Xiao-Fei1,XU Ying1,SUN Zhi-Min1   

  1. 1 Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences / Key Laboratory of Stem fiber Biomass and Engineering Microbilogy, Ministry of Agriculture, Changsha 420105, China; 2 Yiyang Medical School, Yiyang 413000, China; 3 School of Biological Science and Technology, Central South University, Changsha 410205, China
  • Received:2011-10-13 Revised:2012-01-19 Published:2012-05-12 Published online:2012-03-05
  • Contact: 陈建华, E-mail: cjhbt@sina.com, Tel: 13908494958; 栾明宝, E-mail: luanmingbao2002@126.com, Tel:0731-88998518

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被引次数

26

摘要： 分别采用RSAP、SRAP和SSR 3种分子标记和田间性状对16份苎麻种质进行亲缘关系聚类，结果表明, 每对引物扩增出的多态性位点，SRAP标记最多，RSAP次之，SSR较少；根据SRAP标记和RSAP标记分类的结果都与RSAP+SRAP+SSR联合分类的结果基本一致，相关达到了极显著水平，而与SSR标记分类结果差异较大；分子标记聚类结果与田间性状的聚类结果接近程度, 依次为SRAP+RSAP+SSR>SRAP>RSAP>>SSR。在检测苎麻种质亲缘关系中，SRAP标记效果最优，RSAP标记稍逊，SSR标记最差。RSAP标记能较好地显示苎麻种属间的多态性和亲缘关系。以RSAP、SRAP、SSR标记联合分析，能更好地揭示种质之间的亲缘关系。

关键词: 苎麻, RSAP, SRAP, SSR, 亲缘关系

Abstract: Ramie [Boehmeria nivea (L.) Gaud.] researches in molecular biology lags behind, there is a necessary to develop molecular markers in the genetic relationship analysis of ramie. In this paper, three molecular markers-RSAP, SRAP and SSR were used to cluster 16 Ramie germplasm. The result showed that each pair of primers could provide polymorphic sites and the site number was SRAP>RSAP>>SSR. Both results clustered based on SRAP and RSAP showed high consistency with that clustering based on combination of SRAP, RSAP and SSR, and the highly significant correlation coefficients were given. However, results of clustering based on SSR marker were quite different from the results based on combined markers. The rank order of similarities between the clustering results based on the molecular marker and phenotype was SRAP+RSAP+SSR>SRAP>RSAP>>SSR. For the study of genetic relationship among ramie germplasm, the efficiency of SRAP marker was slightly higher than that of RSAP marker, but that of SSR was low. Based on RSAP marker could efficiently display the polymorphism and genetic relationship among ramie varieties. Using the combined markers of RSAP&SRAP&SSR could be to reveal the genetic relationship of germplasm.

Key words: Ramie, RSAP, SRAP, SSR, Relationship

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