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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1607-1616.doi: 10.3724/SP.J.1006.2012.01607

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆GmNF-YC2基因的克隆与功能分析

曹岩1，张晓玫2，陈新建1,*，傅永福2,*   

  1. 1 河南农业大学生命科学学院, 河南郑州450002; 2 农业部北京大豆生物学重点实验室 / 农作物基因资源与遗传改良国家重大科学工程 / 中国农业科学院作物科学研究所, 北京100081
  • 收稿日期:2012-02-27 修回日期:2012-04-20 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 陈新建, E-mail: xinjian@371.net; 傅永福, E-mail: fufu19cn@163.com, Tel: 010-82105864
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2011ZX08009-001, 2011ZX08004-005)和国家重点基础研究发展计划(973计划)项目(2010CB125906)。

Cloning and Functional Analysis of GmNF-YC2 Gene in Soybean (Glycine max)

CAO Yan1,ZHANG Xiao-Mei2,CHEN Xin-Jian1,*,FU Yong-Fu2,*   

  1. 1 College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China; 2 Key Laboratory of Soybean Biology / National Key Facility of Crop Gene Resource and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 10081, China
  • Received:2012-02-27 Revised:2012-04-20 Published:2012-09-12 Published online:2012-07-03
  • Contact: 陈新建, E-mail: xinjian@371.net; 傅永福, E-mail: fufu19cn@163.com, Tel: 010-82105864

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摘要： NF-Y (nuclear factor-y)是真核生物中普遍存在的一种转录因子复合物, 是由3个不同的亚基(A, B, C)组成, 并通过作用于其他调控因子的启动子来调节目的基因的表达。本文从大豆KN18中克隆大豆NF-YC基因GmNF-YC2, 并且利用实时荧光定量PCR (qRT-PCR)分析其表达模式, 发现GmNF-YC2的表达受光调控, 并在开花期第3复叶中表达量最高。结果表明, GmNF-YC2在大豆光周期开花调节中起重要作用。拟南芥原生质体瞬时表达实验证实, GmNF-YC2蛋白定位在细胞核中。这为大豆NF-YC家族基因的功能研究提供重要依据。

关键词: NF-Y转录因子, 开花, CONSTANS, 光周期, 大豆

Abstract: The eukaryotic nuclear factor Y (NF-Y) is a ubiquitous transcription factor complex composed of three distinct subunits (NF-YA, NF-YB, and NF-YC).NF-Y transcription factors typically act on the promoters of other regulatory factors to modulate gene expressions. Here, a NF-YC homologue, GmNF-YC2, was cloned from soybean variety KN18. The expression profiles checked by quantitative Real-time RT-PCR (qRT-PCR) showed that GmNF-YC2 was regulated by light but not by circadian clock. And GmNF-YC2 was expressed at the highest level in third trifoliolates at flowering. The results suggested that GmNF-YC2 was one of the important genes in the regulation of flowering in soybean. The transient expression in Arabidopsis protoplasts displayed that GmNF-YC2 protein was localized in nuclear section. It paved a way to study the function of NF-Y family in soybean and its application in soybean molecular breeding.

Key words: NF-Y transcription factor, Flowering, CONSTANS, Photoperiod, Glycine max

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