作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1839-1846.doi: 10.3724/SP.J.1006.2012.01839
刘玲玲1,2,柳思思2,4,翁建峰2,王昌涛3,李新海2,张世煌2,石庆华4,王丽娟1,*,郝转芳2,*
LIU Ling-Ling1,2,LIU Si-Si2,4,WENG Jian-Feng2,WANG Chang-Tao3,LI Xin-Hai2,ZHANG Shi-Huang2,SHI Qing-Hua4,WANG Li-Juan1,*,HAO Zhuan-Fang2,*
摘要:
HSP90是普遍存在于原核和真核细胞中的一种高度保守的分子伴侣。本研究从玉米中克隆了一个HSP90同源基因, 命名为ZmHSP90-1基因, 并对其进行了初步的序列分析。该基因cDNA序列全长2 371 bp, 开放阅读框2 094 bp, 编码697个氨基酸, 蛋白质分子量约79.98 kD。蛋白结构预测及同源比对分析表明, ZmHSP90-1基因编码蛋白含ATPase位点和HSP90保守结构域, 并与拟南芥、水稻等多种物种的热激蛋白高度同源; 进化树分析表明ZmHSP90-1与拟南芥AtHSP90.1基因关系较近, 蛋白序列相似性达88.3%。目的蛋白亚细胞定位显示, ZmHSP90-1蛋白在细胞质中表达。实时荧光定量PCR分析表明, ZmHSP90-1对非生物胁迫高温、高盐、ABA、低温、干旱均具有明显的应答反应。推测ZmHSP90-1是玉米的一个胁迫相关基因。
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