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作物学报 ›› 2012, Vol. 38 ›› Issue (11): 1949-1959.doi: 10.3724/SP.J.1006.2012.01949

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

粳稻超优1号背景回交导入系的耐热性筛选与评价

孟丽君1,2，马秀芳3,**，唐志强3，沈枫3，崔彦茹1，柴路1，陈凯1，徐建龙1,*，黎志康1,*
   

  1. 1 沈阳农业大学 / 农业部作物生理生态和遗传育种重点实验室, 辽宁沈阳110161; 2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京100081; 3辽宁省农业科学院稻作研究所, 辽宁沈阳110101
  • 收稿日期:2012-03-29 修回日期:2012-06-10 出版日期:2012-11-12 网络出版日期:2012-09-01
  • 通讯作者: 徐建龙, E-mail: xujlcaas@yahoo.com.cn; 黎志康, E-mail: zhkli1953@126.com
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目[2011-G2B(2)]和国家高技术研究发展计划(863计划)项目(2012AA101101)资助。

Screening and Evaluation of Heat Tolerance of Introgression Lines with Japonica Chaoyou 1 Background

MENG Li-Jun1,2,MA Xiu-Fang3,**,TANG Zhi-Qiang3,SHEN Feng3,CUI Yan-Ru1,CAI Lu1,CHEN Kai1,XU Jian-Long1,*,LI Zhi-Kang1,*   

  1. 1 Shenyang Agricultural University / Key Laboratory of Crop Physiology, Ecology, Genetics and Breeding, Ministry of Agriculture, Shenyang 110161, China;
    2 Institute of Crop Sciences / National Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Rice Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110101, China
  • Received:2012-03-29 Revised:2012-06-10 Published:2012-11-12 Published online:2012-09-01
  • Contact: 徐建龙, E-mail: xujlcaas@yahoo.com.cn; 黎志康, E-mail: zhkli1953@126.com

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被引次数

5

摘要：

全球气候变暖不可避免地影响水稻的生产。本研究以粳稻品种超优1号为轮回亲本与9个来自不同国家的供体品种杂交培育的BC2F4回交导入群体为材料, 通过开花期高温胁迫, 共初筛到124个耐高温单株; 以粳稻为供体的回交导入群体出现耐热个体的频率高于籼稻供体的导入群体, 表明粳稻资源中同样存在耐热有利基因。经对初筛后代的耐热性重复鉴定和在正常条件下的性状评价, 发现有80个株系的结实率显著高于轮回亲本, 耐热性选择效率为64.5%, 这些耐热导入系的产量及其相关性状在高温胁迫和正常条件下均出现广幅分离, 从中鉴定出耐热性和产量性状均显著好于轮回亲本的8个优良导入系。在3个耐热导入系的聚合F2群体中, 筛选出耐热个体的平均结实率在80%以上, 极显著高于轮回亲本和最高聚合亲本, 从中获得106株耐热性极显著好于聚合亲本的单株, 显示出较理想的耐热性聚合效果。通过对耐热导入系和轮回亲本在正常和高温胁迫条件下的产量以及相关性状的表型比较分析, 有助于提高对水稻耐热性状筛选中的供体选择、选择效率和筛选方法等一些重要问题的认识。本研究获得的高产耐热聚合系, 将为水稻耐热有利基因发掘和耐热性标记辅助选择聚合育种提供宝贵材料。

关键词: 水稻, 耐热性, 高温胁迫, 导入系, 聚合育种

Abstract:

Global climatic warming inevitably affects rice production. Nine BC2F4 backcross introgression populations derived from crosses between a japonica variety Chaoyou1 (CY1) as the recurrent parent (RP) and nine donor parents collected from different countries were screened for heat tolerance (HT) at flowering stage. A total of 124 introgression lines (ILs) were selected based on seed setting rate in single plant under high temperature (38℃). The results showed that: introgression lines (ILs) had better HT than Chaoyou1, and the frequency of plants with HT derived from the ILs with donors of japonica was higher than that with donors of indica, demonstrating the potential of using genes from japonica varieties to improve HT of breeding line. Progeny testing of the 124 ILs under heat stress and normal condition revealed a high selection efficiency of 64.5% for HT and 80 ILs had higher SF than RP. The 124 selected ILs presented wide segregations for the tested traits in replicated experiments under stress and normal conditions and allowed us to identify eight promising ILs that had greatly improved HT and higher yield than CY1. HT-tolerant plants selected from three pyramiding populations had an average SF of more than 80%, which was significantly higher than that of RP and the highest pyramiding parent. From them, 106 plants with significantly better HT than pyramiding parent were selected, showing ideal pyramiding effect on HT. Detailed comparisons between the ILs and CY1 for yield performances and related traits under heat stress and non-stress conditions provided useful information and better understanding in donor selection, selection efficiency and screening method for HT in the BC breeding process. The selected lines with HT will become valuable materials for gene mining and molecular breeding for improvement of HT by high efficient pyramiding of favorable genes.

Key words: Rice (Oryza sativa L.), Heat tolerance, High temperature stress, Introgression line, Pyramiding breeding

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