作物学报 ›› 2013, Vol. 39 ›› Issue (02): 222-229.doi: 10.3724/SP.J.1006.2013.00222
李伟1,2,商海红2,王少干2,范森淼2,李俊文2,刘爱英2,石玉真2,龚举武2,巩万奎2,王涛2,白志川1,*,袁有禄2,*
LI Wei1,2,SHANG Hai-Hong2,WANG Shao-Gan2,FAN Sen-Miao2,LI Jun-Wen2,LIU Ai-Ying2,SHI Yu-Zhen2,GONG Ju-Wu2,GONG Wan-Kui2,WANG Tao2,BAI Zhi-Chuan1,*,YUAN You-Lu2,*
摘要:
从陆地棉SSH-cDNA文库的测序结果中得到一条具有完整ORF的陆地棉水孔蛋白基因序列,将其命名为GhAQP2。以该基因编码的氨基酸序列为探针,在棉花EST数据库经同源搜索得到2个相似性较高的EST,利用RACE技术获得其全长cDNA序列,将其基因命名为GhAQP3和GhAQP4。基因结构分析发现GhAQP2和GhAQP3各有4个外显子,3个内含子;GhAQP4有3个外显子,2个内含子。生物信息分析表明3个基因编码蛋白均含有6个跨膜区,2个NPA结构域,其氨基酸序列具备MIP超家族典型的蛋白保守区序列特征。多序列比对发现3个基因的氨基酸序列与其他物种PIP2类水孔蛋白氨基酸序列具有很高的同源性。qRT-PCR分析表明,GhAQP2在纤维伸长后期优势表达,GhAQP3在下胚轴和子叶中高表达,GhAQP4在纤维伸长前期优势表达,推测3个基因在不同的组织中发挥作用。GhAQP2在20DPA优势表达,为研究该基因在纤维伸长向次生壁加厚期转化过程中的表达调控提供了重要信息。
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