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作物学报 ›› 2013, Vol. 39 ›› Issue (02): 238-248.doi: 10.3724/SP.J.1006.2013.00238

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

应用SSR分子标记分析小麦品种(系)的遗传重组

李小军,冯素伟,李淦,董娜,陈向东,宋杰, 茹振钢*   

  1. 河南科技学院小麦中心 / 河南省高校作物分子育种重点开放实验室, 河南新乡453003
  • 收稿日期:2012-07-30 修回日期:2012-10-09 出版日期:2013-02-12 网络出版日期:2012-12-11
  • 通讯作者: 茹振钢, E-mail: rzgh58@sohu.com
  • 基金资助:

    本研究由国家科技支撑计划项目(2011BAD07B02), 河南省重大科技专项(111100110100)和国家高技术研究发展计划(863计划)项目(2009AA101102)资助。

Genetic Recombination in Wheat Using SSR Markers

LI Xiao-Jun,FENG Su-Wei,LI Gan,DONG Na,CHEN Xiang-Dong,SONG Jie,RU Zhen-Gang*   

  1. Center of Wheat Breeding, Henan Institute of Science and Technology / Key Discipline Open Laboratory on Crop Molecular Breeding of Henan Institute, Xinxiang 453003, China
  • Received:2012-07-30 Revised:2012-10-09 Published:2013-02-12 Published online:2012-12-11
  • Contact: 茹振钢, E-mail: rzgh58@sohu.com

摘要:

为了解小麦品种形成中亲本基因组的遗传重组和遗传保留区段的分布特点,对周麦18和百农AK58及其衍生品系共23个材料进行了全基因组SSR扫描分析。遗传重组分析表明,单交组合的平均重组数(12.3)低于回交组合(13.9);染色体4A5A7A1B3B4B7B1D2D3D5D6D7D重组发生较多,其余染色体重组相对较少,染色体的中间区段与远端区段重组数相当,分别为6.16.0。子代间基因组比较发现,一些染色体区段成为重组的多发区,如5Dgwm358–wmc3576Dcfd49–barc1967Awmc158–barc237Bgwm274–gwm146区段,分别有35191514次重组。分析亲本传递给子代的染色体区段,发现子代继承亲本的遗传区段在14~29个,每个区段涉及2~8个多态性位点,大的遗传区段主要分布于4A5A5B5D7D染色体。以上基因组区域的关联性状是进一步研究的重点。

关键词: 小麦, 遗传重组, 染色体区段, SSR

Abstract:

To understand the characteristics of inheritance and recombination of parental chromosome fragments in wheat progenies, we screened the genomes of 23 genotypes derived from Zhoumai 18 and Bainong AK58 with 340 SSR markers covering the whole wheat genome, together with the parents. The average recombination frequency in cultivars from single-cross was 12.3, which was smaller than that in cultivars from single backcross (13.9). Recombination mostly occurred on chromosomes 4A, 5A, 7A, 1B, 3B, 4B, 7B, 1D, 2D, 3D, 5D, 6D, and 7D. The distal and central chromosomal regions had similar frequencies of recombination which were 6.1, and 6.0, respectively. Some chromosomal regions were hot in recombination, such as marker intervals gwm358–wmc357 on chromosome 5D, cfd49–barc196 on chromosome 6D, wmc158–barc23 on chromosome 7A, and gwm274–gwm146 on chromosome 7B, with 35, 19, 15, and 14 recombination events, respectively. The analysis for inheritance of large linkage blocks indicated that large chromosome fragments inherited from one parent varied from 14 to 29 in each derivative, with 2–8 consecutive and informative SSR loci in a fragment. These large fragments were mainly distributed on chromosomes 4A, 5A, 5B, 5D, and 7D, which might harbor genes controlling important agronomic traits.

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