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作物学报 ›› 2013, Vol. 39 ›› Issue (12): 2177-2182.doi: 10.3724/SP.J.1006.2013.02177

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米胁迫诱导表达基因ZmSNAC1的功能分析

卢敏,张登峰,石云素,宋燕春,黎裕*,王天宇*   

  1. 中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2013-03-27 修回日期:2013-06-24 出版日期:2013-12-12 网络出版日期:2013-08-12
  • 通讯作者: 黎裕, E-mail: liyu03@caas.cn; 王天宇, E-mail: wangtianyu@263.net
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2011ZX08003-004)，国家科技支撑计划项目(2013BAD01B02)和国家自然科学基金项目(U1138304)资助。

Overexpression of a Stress Induced Maize NAC Transcription Factor Gene, ZmSNAC1, Improved Drought and Salt Tolerance in Arabidopsis

LU Min,ZHANG Deng-Feng,SHI Yun-Su,SONG Yan-Chun,LI Yu*,WANG Tian-Yu*   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI), Beijing 100081, China
  • Received:2013-03-27 Revised:2013-06-24 Published:2013-12-12 Published online:2013-08-12
  • Contact: 黎裕, E-mail: liyu03@caas.cn; 王天宇, E-mail: wangtianyu@263.net

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摘要：

NAC转录因子是具有多种生物功能的植物特异转录调控因子，在植物生长发育、器官建成、激素调节和抵抗逆境等方面发挥着重要的作用。我们之前分离得到1个受干旱、盐、冷等非生物逆境胁迫和植物激素ABA诱导显著上调表达的玉米NAC家族成员ZmSNAC1，过表达转基因株系在苗期的耐脱水能力较野生型株系明显提高。在此基础上，本研究进一步对ZmSNAC1过表达转基因株系在植株生殖生长发育时期的抗旱性和耐盐性进行功能鉴定。结果表明，在干旱胁迫条件下，野生型株系相比转基因株系较存活率提高50%~52%，相对电导率降低17%~21%，叶绿素含量提高36%~47%，脯氨酸含量提高了17%~23%；在300mmol L-1 NaCl胁迫条件下，转基因株系的存活率提高36~40%。ZmSNAC1可能作为一个正向调控因子在逆境胁迫信号转导过程中发挥重要作用。

关键词: ZmSNAC1, 逆境胁迫抗逆, 玉米

Abstract:

NAC proteins, which are important regulatory factors, have received much attention for their biological functions in abiotic stress adaptations and tolerances, as well as in the plant metabolic processes under biotic stress and in development. Previously, we isolated a stress-induced NAC gene, designed as ZmSNAC1, from the maize inbred line “CN165” and found that overexpression of ZmSNAC1 led to enhanced tolerance to dehydration at seedling stage. In this study, the functions of ZmSNAC1 were further characterized under drought and salt stresses at reproductive stage. The results indicate that overexpression of ZmSNAC1 inArabidopsis conferred enhanced drought and salt tolerances, showing an improved survival percentage and physiological changes, such as the reduced electrolyte leakage and higher chlorophyll content in transgenic plants. These results indicated that ZmSNAC1 may function as a positive regulator in multiple pathways of signal transduction under abiotic stresses and have potential utilization in transgenic breeding for improving stress tolerance in crops.

Key words: ZmSNAC1, Abiotic stress, Stress tolerance, Maize

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