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作物学报 ›› 2015, Vol. 41 ›› Issue (02): 187-196.doi: 10.3724/SP.J.1006.2015.00187

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆籽粒维生素E含量的QTL分析

张红梅1,李海朝2,文自翔2,顾和平1,袁星星1,陈华涛1,崔晓艳1,陈新1,*,卢为国2,*   

  1. 1江苏省农业科学院蔬菜研究所，江苏南京 210014；2河南省农业科学院经济作物研究所 / 国家大豆改良中心郑州分中心 / 农业部黄淮海油料作物遗传育种重点实验室，河南郑州 450002
  • 收稿日期:2014-06-25 修回日期:2014-12-19 出版日期:2015-02-12 网络出版日期:2014-12-29
  • 通讯作者: 陈新, E-mail: cx@jaas.ac.cn, Tel: 025-84391362; 卢为国, E-mail: 123bean@163.com, Tel. 0371-65733647
  • 基金资助:

    本研究由国家自然科学基金项目(30771360)，江苏省农业自主创新基金项目[cx(13)4070]和江苏省科技支撑计划项目(BE2013379)资助。

Identification of QTL Associated with Vitamin E Content in Soybean Seeds

ZHANG Hong-Mei1,LI Hai-Chao2,WEN Zi-Xiang2,GU He-Ping1,YUAN Xing-Xing1,CHEN Hua-Tao1,CUI Xiao-Yan1,CHEN Xin1,*,LU Wei-Guo2,*   

  1. 1 Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2 Institute of Industrial Crops, Henan Academy of Agricultural Scienecs / Zhengzhou Subcenter of National Center for Soybean Improvement / Key laboratory of Oil Crops in Huanghuaihai Plains, Ministry of Agriculture, Zhengzhou 450002, China
  • Received:2014-06-25 Revised:2014-12-19 Published:2015-02-12 Published online:2014-12-29
  • Contact: 陈新, E-mail: cx@jaas.ac.cn, Tel: 025-84391362; 卢为国, E-mail: 123bean@163.com, Tel. 0371-65733647

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摘要：

维生素E (VE)具有提高人体免疫力、抗癌、预防心血管疾病等保健作用，从大豆中提取的VE安全性更高。本研究采用高效液相色谱技术(HPLC)检测大豆BIEX群体(Essex×ZDD2315)维生素E的α-生育酚、γ-生育酚和δ-生育酚含量。应用QTLNetwork 2.1软件分别检测到8个和12对控制大豆维生素E及组分含量的加性和互作QTL。α-生育酚含量加性和互作QTL累计贡献值分别为8.68% (2个)和15.57% (4对)，γ-生育酚含量加性和互作QTL累计贡献值分别为8.59% (2个)和11.57% (2对)，δ-生育酚含量加性和互作QTL累计贡献值分别为5.44% (1个)和17.61% (3对)，维生素E总含量的加性和互作QTL累计贡献值分别为11.39% (3个)和9.48% (3对)。未检测到维生素E及组分含量和环境互作的QTL。未定位到的微效QTL累计贡献值为66.16%~75.32%，说明未定位到的微效基因的变异占2/3以上。各性状的遗传构成中，未检测出的微效QTL份额最大，加性QTL和互作QTL贡献相差不大。在育种中应考虑常规方法聚合微效QTL与标记辅助方法聚合主要QTL相结合。

关键词: 大豆, 籽粒, 维生素E含量, QTL定位

Abstract:

Vitamin E has effects on human immunity, anti-cancer and prevention of cardiovascular disease. Vitamin E from soybean has the advantages of higher security and higher human body absorption rate. The objective of the present study was to map the additive，additive × additive (epistasis), additive × year and epistasis × year QTLs for vitamin E and relative tocopherol contents with the RIL population BIEX (Essex×ZDD2315) using HPLC (high performance liquid chromatography) method and software QTLNetwork 2.1. Eight additive QTLs and twelve additive × additive (epistasis) QTLs were detected for vitamin E and relative tocopherol contents. The contributions to the phenotypic variances of additive QTL and epistatic QTL pairs were 8.68% (two QTLs) and 15.57% (four pairs) for α- tocopherol, 8.59% (two QTL) and 11.57% (two pairs) for γ-tocopherol, 5.44% (one QTL) and 17.61% (three pairs) for δ-tocopherol and 11.39% (three QTL) and 9.48% (three pairs) for total vitamin E contents, respectively. Those of additive and epistatic QTLs by year interaction were not found. The accumulated contribution of the unmapped minor QTLs was 66.16%–75.32%, indicating the variance of unmapped minor QTLs accounting for more than two thirds. In genetic composition, undetected minor QTLs accounted for a considerably large part additive QTLs and epistatic QTLs were nearly equal in α-tocopherol, γ-tocopherol, δ-tocopherol and total vitamin E contents. Accordingly, in breeding for vitamin E contents, the strategy of pyramiding multiple QTLs, both additive and epistatic, by using marker-assisted selection combined with accumulating minor effect QTLs through conversional procedures should be considered.

Key words: Soybean [Glycine max (L.) Merr.], Seed, Vitamin E content, QTL mapping

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