欢迎访问作物学报,今天是

作物学报 ›› 2015, Vol. 41 ›› Issue (06): 838-844.doi: 10.3724/SP.J.1006.2015.00838

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻白穗突变体wp4的鉴定与基因精细定位

王晓雯,蒋钰东,廖红香,杨波,邹帅宇,朱小燕,何光华*,桑贤春*   

  1. 西南大学水稻研究所 / 转基因植物与安全控制重庆市重点实验室 / 南方山地农业教育部工程研究中心, 重庆 400716
  • 收稿日期:2014-11-24 修回日期:2015-03-19 出版日期:2015-06-12 网络出版日期:2015-04-17
  • 通讯作者: 桑贤春, E-mail: sangxianchun@163.com; 何光华, E-mail: hegh@swu.edu.cn
  • 基金资助:

    本研究由中央高校基本科研业务费(XDJK2011c016)和国家自然科学基金项目(31171178)资助。

Identification and Gene Fine Mapping of White Panicle Mutant wp4 in Oryza sativa

WANG Xiao-Wen,JIANG Yu-Dong,LIAO Hong-Xiang,YANG Bo,ZOU Shuai-Yu,ZHU Xiao-Yan,HE Guang-Hua*,SANG Xian-Chun*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2014-11-24 Revised:2015-03-19 Published:2015-06-12 Published online:2015-04-17
  • Contact: 桑贤春, E-mail: sangxianchun@163.com; 何光华, E-mail: hegh@swu.edu.cn

摘要:

水稻开花灌浆期,内外颖呈绿色,含光合色素,为阐释非叶片组织叶绿体发育的分子机制,本文对EMS诱变获得的新型白穗突变体wp4进行了研究。抽穗灌浆期,wp4的穗轴呈绿色,内外颖呈乳白色;内外颖叶绿体发育不健全,无类囊体结构,叶绿素a、叶绿素b和类胡萝卜素含量极显著降低。此外,wp4全生育期叶片呈淡黄色,叶绿体内基质片层较少且排列松散;与野生型相比,wp4的叶绿素a含量显著降低,叶绿素b和类胡萝卜素含量虽略低,但差异未达到显著水平。农艺性状分析发现,与野生型相比,wp4的有效穗和结实率略有增加,其他性状则略有降低,但变化均未达到显著差异水平。遗传分析表明wp4受一对隐性核基因调控,利用1200株西农1A/wp4的F2隐性群体,最终将WP4精细定位在第8染色体约79 kb的物理范围内,根据水稻基因组注释计划,该区间包含14个基因,这为WP4的功能和作用机制研究奠定了基础,也为水稻标记性状育种提供了新的资源。

关键词: 水稻(Oryza sativa), 白穗黄绿叶, 基因定位, 叶色标记

Abstract:

At the flowering and filling stages, rice hull appears green color and possesses photosynthetic pigment. To clear the mechanism of chloroplast development in the panicle, we identified a novel white panicle mutant from the progeny of indica restorer line Jinhui 10 with seeds treated by EMS and termed it as wp4. The wp4 displayed green spike-stalks and milk-white hulls after the heading stage. The structures of chloroplast and thylakoid were severely destroyed and the contents of photosynthetic pigment decreased extremely significantly in the mutational hulls. Compared with the wild type, the wp4 displayed yellow green leaves and contained looser stromal lamellae. And the contents of chlorophyll a, b, and carotenoid were all declined while only the changing of chlorophyll a led to the significantly different level in statistics. Except for effective panicles number and seed setting rate, other detected agronomic characteristics decreased slightly but the changing did not come up to the statistically significant difference. Genetic analysis indicated that the white panicle of wp4 was controlled by a recessive nuclear gene and which was finally mapped on chromosome 8 with 79 kb physical distances according to 1200 mutational plants derived from the F2 generation of Xinong1A/wp4. The restricted region contained 14 annotated genes based on Rice Genome Annotation Project. These results provided a foundation for gene cloning and function analysis of the WP4. Meanwhile, the traits of wp4 could be available in rice breeding as a morphological marker.

Key words: Rice (Oryza sativa)White panicle, Yellow green leaf, Gene mapping, Leaf color marker

[1]宋桂云, 徐正进, 苏慧, 王翠花, 宫雅琴, 孙海燕. 不同穗型的两个水稻品种株型的研究. 内蒙古民族大学学报(自然科学版), 2006, 21(3): 294–299

Song J Y, Xu Z J, Su H, Wang C H, Gong Y Q, Sun H Y. The study on plant shape of different panicle rice varieties. J Inner Mongolia Univ Nationalities (Nat Sci), 2006, 21(3): 294–299 (in Chinese with an English abstract)

[2]段俊, 田长恩, 梁承邺. 水稻结实过程中谷壳的作用及生理变化. 作物学报, 2000, 26: 71–76

Duan J, Tian C E, Liang C Y. Studies on effects and physiological changes of the hull on grain-filling in rice. Acta Agron Sin, 2000, 26: 71–76 (in Chinese with an English abstract)

[3]邓晓娟, 张海清, 王悦, 舒志芬, 王国槐, 王国梁. 水稻叶色突变基因研究进展. 杂交水稻, 2012, 27(5): 9–14

Deng X J, Zhang H Q, Wang Y, Shu Z F, Wang G H, Wang G L. Research advances on rice leaf-color mutant genes. Hybrid Rice, 2012, 27(5): 9–14 (in Chinese with an English abstract)

[4]Tanaka A, Tanaka R. Chlorophyll metabolism. Curr Opin Plant Biol, 2006, 9: 248–255

[5]Pogson B J, Albrecht V. Genetic dissection of chloroplast biogenesis and development: an overview. Plant Physiol, 2011, 155: 1545–1551

[6]Sanchez A C, Khush G S. Chromosomal location of some marker genes in rice using primary trisomics. J Hered, 1994, 85: 297–300

[7]Song J, Wei X J, Shao G N, Sheng Z H, Chen D B, Liu C L, Jiao G A, Xie L H, Tang S Q, Hu P S. The rice nuclear gene WLP1 encoding a chloroplast ribosome L13 protein is needed for chloroplast development in rice grown under low temperature conditions. Plant Mol Biol, 2014, 84: 301–314

[8]金怡, 刘合芹, 汪得凯, 陶跃之. 一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位. 中国水稻科学, 2011, 25: 461–466

Jin Y, Liu H Q, Wang D K, Tao Y Z. Genetic analysis and gene mapping of a white striped leaf and white panicle mutant in rice. Chin J Rice Sci, 2011, 25(5): 461–466 (in Chinese with English abstract)

[9]Li H C, Qian Q, Wang Y, Li X B, Zhu L H, Xu J C. Characterization and mapping of a white panicle mutant gene in rice. Chin Sci Bull, 2003, 48(3): 268–270

[10]陈德西, 李婷, 曲广林, 黄文娟, 何忠全, 李仕贵. 水稻条斑和颖花异常突变体st-fon的鉴定与遗传分析. 中国水稻科学, 2012, 26: 677–685

Chen D X, Li T, Qu G L, Huang W J, He Z Q, Li S G. Characterization and genetic analysis of a streaked and abnormal glumous flower mutant st-fon. Chin J Rice Sci, 2012, 26: 677–685 (in Chinese with English abstract)

[11]Wellburn A R. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Plant Physiol, 1994, 144: 307–313

[12]Fang L K, Li Y F, Gong X P, Sang X C, Ling Y H, Wang X W, Cong Y F, He G H. Genetic analysis and gene mapping of a dominant presenescing leaf gene PSL3 in rice (Oryza sativa L.). Chin Sci Bull, 2010, 55: 1676–1681

[13]Richly E, Leister D. An improved prediction of chloroplast proteins reveals diversities and commonalities in the chloroplast proteomes of Arabidopsis and rice. Gene, 2004, 329: 11-16

[14]Park J H, Jensen B C, Kifer C T, Parsons M. A novel nucleolar G-protein conserved in eukaryotes. J Cell Sci, 2000, 114: 173–185

[15]Honma Y, Kitamura A, Shioda R, Maruyama H, Ozaki K, Oda Y, Mini T, Jenö P, Maki Y, Yonezawa K, Hurt E, Ueno M, Uritani M, Hall M N, Ushimaru T. TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients. EMBO J, 2006, 25: 3832–3842

[16]Jensen B C, Wang Q, Kifer C T, Parsons M. The NOG1 GTP-binding protein is required for biogenesis of the 60S ribosomal subunit. J Biol Chem, 2003, 278: 32204–32211

[17]Lo KY, Li Z H, Bussiere C, Bresson S, Marcotte E M, Johnson A W. Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit. Mol Cell, 2010, 39: 196–208

[18]Jeon Y, Ahn C S, Jung H J, Kang H, Park G T, Cho Y, Hwang J, Pai H. DER containing two consecutive GTP-binding domains plays an essential role in chloroplast ribosomal RNA processing and ribosome biogenesis in higher plants. J Exp Bot, 2014, 65: 117–130

[19]郭涛, 黄宣, 黄永相, 刘永柱, 张建国, 陈志强, 王慧. 水稻叶色白化转绿及多分蘖矮秆基因hw-1(t)的图位克隆. 作物学报, 2012, 38: 23–35

Guo T, Huang X, Huang Y X, Liu Y Z, Zhang J G, Chen Z Q, Wang H. Characterizations of a mutant gene hw-1(t) for green-revertible albino, high tillering and dwarf in rice (Oryza sativa L.). Acta Agron Sin, 2012, 38: 23–35 (in Chinese with English abstract)

[20]Zhang F T, Luo X D, Hu B L, Wan Y, Xie J K. YGL138(t), encoding a putative signal recognition particle 54 kDa protein, is involved in chloroplast development of rice. Rice, 2013, 6: 7

[21]Wu Z M, Zhang X, He B, Diao L P, Sheng S L, Wang J L, Guo X P, Su N, Wang L F, Jiang L, Wang C M, Zhai H Q, Wan J M. A chlorophyll deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol, 2007, 145: 29–40

[22]Chen H, Cheng Z J, Ma X D, Wu H, Liu Y L, Zhou K N, Chen Y L, Ma W W, Bi J C, Zhang X, Guo X P, Wang J L, Lei C L, Wu F Q, Lin Q B, Liu Y Q, Liu L L, Jiang L. A knockdown mutation of YELLOW-GREEN LEAF2 blocks chlorophyll biosynthesis in rice. Plant Cell Rep, 2013, 32: 1855–1867

[23]刘梦梦, 桑贤春, 凌英华, 杜鹏, 赵芳明, 杨正林, 何光华. 水稻黄绿叶基因YGL4的遗传分析和分子定位. 作物学报, 2009, 35: 1405–1409

Liu M M, Sang X C, Ling Y H, Du P, Zhao F M, Yang Z L, He G H. Genetic analysis and molecular mapping of a yellow green leaf gene (YGL4) in rice (Oryza sativa L.). Acta Agron Sin, 2009, 35: 1405–1409 (in Chinese with English abstract)

[24]杨海莲, 刘敏, 郭旻, 李荣德, 张宏根, 严长杰. 一个水稻黄绿叶突变体ygl10的遗传分析和基因定位. 中国水稻科学, 2014, 28(1): 41–48

Yang H L, Liu M, Guo M, Li R D, Zhang H G, Yan C J. Genetic analysis and position cloning of a yell-green leaf 10 (ygl10) gene, responsible for leaf color in rice. Chin J Rice Sci, 2014, 28(1): 41–48 (in Chinese with English abstract)

[25]孙小秋, 王兵, 肖云华, 万春美, 邓晓建, 王荣平. 水稻ygl98黄绿叶突变基因的精细定位与遗传分析. 作物学报, 2011, 37: 991–997

Sun X Q, Wang B, Xiao Y H, Wan C M, Deng X J, Wang R P. Genetic analysis and fine-mapping of ygl98 yellow-green leaf gene in rice. Acta Agron Sin, 2011, 37: 991–997 (in Chinese with English abstract)

[1] 李世鹏, 陈才武, 张晶, 吕恬, 傅廷栋, 易斌. 基于改进U-Net++模型的油菜pol TCMS温敏两系育性等级鉴定及温度育性关系的量化研究[J]. 作物学报, 2025, 51(6): 1423-1434.
[2] 杨思杰, 杜启迪, 柴守玺, 熊宏春, 谢永盾, 赵林姝, 古佳玉, 郭会君, 刘录祥. 小麦小旗叶突变性状基因定位与遗传分析[J]. 作物学报, 2025, 51(6): 1548-1557.
[3] 袁鑫, 赵卓凡, 赵瑞清, 刘孝伟, 郑名敏, 刘育生, 董好胜, 邓丽娟, 曹墨菊, 黄强. 一份玉米小籽粒发育突变体mn-like1的遗传分析与分子鉴定[J]. 作物学报, 2025, 51(6): 1569-1581.
[4] 苏帅, 刘孝伟, 牛群凯, 时子文, 侯雨微, 冯开洁, 荣廷昭, 曹墨菊. 玉米多叶矮化突变体lyd1的鉴定与基因克隆[J]. 作物学报, 2024, 50(5): 1124-1135.
[5] 余瑶, 王紫瑶, 周思睿, 刘鹏程, 叶亚峰, 马伯军, 刘斌美, 陈析丰. 水稻类病变突变体lms1的表型鉴定与抗病分子机制分析[J]. 作物学报, 2024, 50(4): 857-870.
[6] 杨晨曦, 周文期, 周香艳, 刘忠祥, 周玉乾, 刘芥杉, 杨彦忠, 何海军, 王晓娟, 连晓荣, 李永生. 控制玉米株高基因PHR1的基因克隆[J]. 作物学报, 2024, 50(1): 55-66.
[7] 唐杰, 龙湍, 吴春瑜, 李新鹏, 曾翔, 吴永忠, 黄培劲. 水稻OsGMS2基因的鉴定及其核不育系种子繁殖体系构建[J]. 作物学报, 2023, 49(8): 2025-2038.
[8] 王兴荣, 张彦军, 涂奇奇, 龚佃明, 邱法展. 一个新的玉米细胞核雄性不育突变体ms6的鉴定与基因定位[J]. 作物学报, 2023, 49(8): 2077-2087.
[9] 林孝欣, 黄明江, 韦祎, 朱洪慧, 王子怡, 李忠成, 庄慧, 李彦羲, 李云峰, 陈锐. 水稻籽粒伸长突变体lgdp的鉴定与基因定位[J]. 作物学报, 2023, 49(6): 1699-1707.
[10] 戴文慧, 朱琪, 张小芳, 吕沈阳, 项显波, 马涛, 陈宇杰, 朱世华, 丁沃娜. 一个水稻脆秆突变体bc21的鉴定和基因定位[J]. 作物学报, 2023, 49(5): 1426-1431.
[11] 严昕, 项超, 刘荣, 李冠, 李孟伟, 李正丽, 宗绪晓, 杨涛. 基于BSA-seq技术对豌豆花色基因的精细定位[J]. 作物学报, 2023, 49(4): 1006-1015.
[12] 李秋平, 张春龙, 杨宏, 王拓, 李娟, 金寿林, 黄大军, 李丹丹, 文建成. 水稻半育突变体sfp10的生理特征分析及基因定位[J]. 作物学报, 2023, 49(3): 634-646.
[13] 曹枭雄, 刘伊凡, 周玉强, 王婧, 吴宇锦, 王红武, 李坤, 刘小刚, 黄长玲, 刘志芳, 郭晋杰, 胡小娇. 一个玉米ZmMs7复等位基因突变体的遗传分析与分子鉴定[J]. 作物学报, 2023, 49(11): 2913-2922.
[14] 委刚, 陈单阳, 任德勇, 杨宏霞, 伍靖雯, 冯萍, 王楠. 水稻细长秆突变体sr10的鉴定与基因定位[J]. 作物学报, 2022, 48(8): 2125-2133.
[15] 杜启迪, 郭会君, 熊宏春, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 宋希云, 刘录祥. 小麦顶端小穗退化突变体asd1基因定位[J]. 作物学报, 2022, 48(8): 1905-1913.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!