欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2015, Vol. 41 ›› Issue (07): 1027-1038.doi: 10.3724/SP.J.1006.2015.01027

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜株高、第一分枝高和分枝数的QTL检测及候选基因筛选

王嘉**,荆凌云**,荐红举,曲存民,谌利,李加纳,刘列钊*   

  1. 西南大学农学与生物科技学院, 重庆 400715
  • 收稿日期:2014-11-25 修回日期:2015-04-02 出版日期:2015-07-12 网络出版日期:2015-04-14
  • 基金资助:

    本研究由国家自然科学基金项目(31371655)资助。

Quantitative Trait Loci Mapping for Plant Height, the First Branch Height, and Branch Number and Possible Candidate Genes Screening in Brassica napus L.

WANG Jia**,JING Ling-Yun**,JIAN Hong-Ju,QU Cun-Min,CHEN Li,LI Jia-Na,LIU Lie-Zhao*   

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
  • Received:2014-11-25 Revised:2015-04-02 Published:2015-07-12 Published online:2015-04-14

RichHTML

PDF

979

被引次数

5

摘要：

株高、分枝数及第1分枝高是油菜重要的农艺性状。本研究利用甘蓝型油菜GH06和P174杂交, F2通过单粒法连续自交至F11构建重组自交系群体, 利用油菜60K芯片对该群体进行基因分型, 构建高密度遗传连锁图谱。结果表明, 该图谱包含2795个SNP多态性标记位点, 总长1832.9 cM, 相邻标记间平均距离为0.66 cM。在此图谱基础上采用复合区间作图法(CIM), 检测到3个农艺性状的24个QTL。其中11个株高QTL分别位于A01、A06、A07、A08、A10和C06染色体, 单个QTL解释5.00%~15.26%的表型变异; 7个第1分枝高QTL分别位于A06、C05和C06染色体, 单个QTL解释5.04%~12.99%的表型变异; 6个分枝数QTL分别位于A03、A07、C01、C04和C06染色体, 单个QTL解释5.95%~8.14%的表型变异。将156个拟南芥株高相关基因、10个拟南芥第1分枝高相关基因和148个拟南芥分枝数相关基因与QTL对应置信区间序列进行同源比较分析(E<1E–20), 分别找出了20个株高候选基因、3个第1分枝高候选基因以及12个分枝数候选基因。2个环境中在A07染色体上重复检测到的QTL置信区间检测到与株高相关的候选基因ATGID1B/GID1B和WRI1, A08染色体上重复检测到的QTL置信区间检测到SLR/IAA14和AXR2/IAA72个与株高相关的候选基因。在具有部分置信区间重叠的q2013FBH-C05-1和q2014FBH-C05-2区间均检测到第1分枝高候选基因PHT1;8, 在A03和C06染色体上的QTL置信区间内, 分别检测到4个分枝数候选基因, 匹配E值介于0~3E–56之间。

关键词: 甘蓝型油菜, 单核苷酸多态性, 数量性状位点, 株高, 第1分枝高度, 分枝数

Abstract:

Plant height, the first branch height and branch number are important agronomic traits in rapeseed. In our study, QTL mapping of plant height, the first branch height and branch number in Brassica napus was tested by using the high density SNP genetic map constructed from the high generation RIL population with the rapeseed 60K chip array. The reference SNP genetic map contains 2795 SNP markers, covering 1832.9 cM with an average distance of 0.66 cM in B. napus genome. Totally, 24 putative QTLs were identified for plant height, the first branch height and branch number by using the complex interval mapping. The phenotypic variation was explained by individual QTL ranged from 5.00% to 15.26% for 11 QTLs of plant height, from 5.04% to 12.99% for seven QTLs of the first branch height, and from 5.95% to 8.14% for six QTLs of branch number. We collected 156 genes associated with plant height, 10 genes associated with the first branch height and 148 genes associated with branch number in Arabidopsis thaliana and searched the homology region of the QTL confidence interval E-value<1E–20 to screen the possible candidate genes. We found 20 genes associated with plant height, three genes associated with the first branch height and 12 genes associated with branch number. The plant height candidate genes ATGID1B/GID1B and WRI1 were found in QTL confidence interval on A07 and SLR/IAA14 and AXR2/IAA7 were found in QTL confidence interval on A08 in 2013 and 2014. The first branch height candidate gene PHT1;8 was found in QTLs q2013FBH-C05-1 and q2014FBH-C05-2 that had an overlapping confidence interval. Moreover, we found four genes associated with branch number that E-value from 0 to 3E–56 on A03 and C06, respectively.

Key words: Brassica napus, SNP, QTL, Plant height, The first branch height, Branch number

[1] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[2] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[3] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[4] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[5] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[6] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[7] 付美玉, 熊宏春, 周春云, 郭会君, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 徐延浩, 刘录祥. 小麦矮秆突变体je0098的遗传分析与其矮秆基因定位[J]. 作物学报, 2022, 48(3): 580-589.
[8] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[9] 王瑞, 陈雪, 郭青青, 周蓉, 陈蕾, 李加纳. 甘蓝型油菜白花基因InDel连锁标记开发[J]. 作物学报, 2022, 48(3): 759-769.
[10] 王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[11] 韩玉洲, 张勇, 杨阳, 顾正中, 吴科, 谢全, 孔忠新, 贾海燕, 马正强. 小麦株高QTL Qph.nau-5B的效应评价[J]. 作物学报, 2021, 47(6): 1188-1196.
[12] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[13] 王吴彬, 童飞, KHAN Mueen Alam, 张雅轩, 贺建波, 郝晓帅, 邢光南, 赵团结, 盖钧镒. 大豆根部水压胁迫耐逆指数遗传体系解析[J]. 作物学报, 2021, 47(5): 847-859.
[14] 唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[15] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2