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作物学报 ›› 2015, Vol. 41 ›› Issue (07): 1098-1104.doi: 10.3724/SP.J.1006.2015.01098

• 耕作栽培·生理生化 • 上一篇    下一篇

套作大豆苗期茎秆木质素合成与抗倒性的关系

邹俊林1,刘卫国1*,袁晋1,蒋涛2,叶素琴1,邓榆川1,杨晨雨1,罗玲1,杨文钰1   

  1. 1 四川农业大学农学院 / 农业部西南作物生理生态与耕作重点实验室，四川成都 611130; 2 西南民族大学生命科学与技术学院，四川成都 610041
  • 收稿日期:2015-01-15 修回日期:2015-04-02 出版日期:2015-07-12 网络出版日期:2015-05-04
  • 基金资助:

    本研究由国家自然科学基金项目(31201170)资助。

Relationship between Lignin Synthesis and Lodging Resistance at Seedlings Stage in Soybean Intercropping System

ZOU Jun-Lin1,LIU Wei-Guo1,*,YUAN Jin1,JIANG Tao2,YE Su-Qin1,DENG Yu-Chuan1,YANG Chen-Yu1,LUO Ling1,YANG Wen-Yu1,*   

  1. 1 College of Agronomy, Sichuan Agricultural University / Key Laboratory of Crop Ecophysiology and Farming System in Southwest China, Ministry of Agriculture, Chengdu 611130, China; 2 College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
  • Received:2015-01-15 Revised:2015-04-02 Published:2015-07-12 Published online:2015-05-04

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摘要：

为从茎秆强度的角度探索套作大豆苗期耐阴抗倒机制，对套作大豆苗期茎秆木质素合成与抗倒性的关系进行了研究。采用耐阴性不同的3个大豆品种(系)，在大豆-玉米套作和大豆单作两种种植模式下，测定茎秆的木质素含量及其合成过程中的苯丙氨酸转氨酶(PAL)、4-香豆酸：CoA连接酶(4CL)、羟基肉桂醇脱氢酶(CAD)、过氧化物酶(POD)等关键酶活性以及茎秆抗折力和抗倒伏指数。结果表明，套作大豆苗期倒伏严重，茎秆抗折力、抗倒伏指数、木质素含量和相关酶活性均显著低于单作。不同大豆品种受套作阴蔽影响程度不同，强耐阴性大豆南豆12茎秆抗折力降低幅度最小，在套作环境下其茎秆抗折力、抗倒伏指数大，茎秆木质素含量高，PAL、4CL、CAD、POD活性强。相关分析表明，套作大豆苗期茎秆木质素含量与抗折力极显著正相关(r = 0.890，P < 0.01)，与倒伏率极显著负相关(r = –0.889，P < 0.01)，与4CL、CAD酶活性显著正相关。套作环境下，强耐阴性大豆苗期茎秆中较高的4CL、CAD活性是其维持高木质素含量的酶学基础，而高木质素含量有利于提高茎秆强度，进而增强其抗倒伏能力。

关键词: 大豆, 套作, 抗倒伏}木质素, 酶活性

Abstract:

Stem physical characteristics are closely related to the crop lodging, increasing stem strength is available to resolve the problem of lodging.To explore the effect of intercropping soybean stem strength on the mechanism of shade tolerance and lodging resistance, we studied the relationship between lignin synthesis and lodging resistance. Three soybean cultivars with different shade tolerances were planted in maize–soybean intercropping and soybean monocropping, respectively. The lignin content, activities of phenylalanine ammonia-lyase (PAL), 4-coumarate:CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD), peroxidase (POD) which involved in lignin synthesis in stem as well as the snapping resistance and lodging resistance index were determined at seedlings stage. The results showed that the intercropping soybean lodged seriously, the snapping resistance, lodging resistance index, lignin content and activities of the four enzymes were significantly lower than those of monocropping soybean at seedlings stage. The three soybean cultivars were affected differently by intercropping condition, the reduction of snapping resistance of Nandou 12 with strong shade tolerance was the least, its lodging resistance index, lignin content and activities of the four enzymes (PAL, 4CL, CAD, POD) also maintained a relatively high level. The lignin content of intercropping soybean seedling stem was significantly positively correlated with the snapping resistance (r = 0.890, P < 0.01) and the activities of 4CL, CAD, and significantly negatively correlated with the actual lodging percentage(r = –0.889, P < 0.01). In a word, the high activities of 4CL and CAD in the soybean stem provide a enzymatic basis to maintain higher lignin content, resulting in higher stem strength and higher lodging resistance in intercropping condition.

Key words: Soybean, Intercropping, Lodging resistance, Lignin, Enzyme activity

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