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作物学报 ›› 2016, Vol. 42 ›› Issue (01): 11-18.doi: 10.3724/SP.J.1006.2016.00011

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦果聚糖合成酶基因6-SFT-D多态性及其与6-SFT-A2的累加效应

岳爱琴1,2,李昂2,毛新国2,昌小平2,柳玉平2,李润植1,景蕊莲2,*   

  1. 1山西农业大学农学院,山西太谷 030801;2中国农业科学院作物科学研究所 / 农业部作物种质资源利用重点开放实验室,北京 100081
  • 收稿日期:2015-09-06 修回日期:2015-09-06 出版日期:2016-01-12 网络出版日期:2015-10-08
  • 通讯作者: 景蕊莲, E-mail: jingruilian@caas.cn, Tel: 010-82105829
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA100501)和国家自然科学基金项目(31461143024)资助。

Sequence Polymorphism and Cumulative Effect with 6-SFT-A2 of Fructan Biosynthesis Gene 6-SFT-D in Wheat

YUE Ai-Qin1, 2,LI Ang2,MAO Xin-Guo2,CHANG Xiao-Ping2,LIU Yu-Ping2,LI Run-Zhi1,JING Rui-Lian2,*   

  1. 1College of Agronomy, Shanxi Agricultural University, Taigu 030801, China; 2Institute of Crop Science, Chinese Academy of Agricultural Sciences / Key Laboratory of Crop Germplasm and Utilization, Ministry of Agriculture, Beijing 100081, China
  • Received:2015-09-06 Revised:2015-09-06 Published:2016-01-12 Published online:2015-10-08
  • Contact: 景蕊莲, E-mail: jingruilian@caas.cn, Tel: 010-82105829
  • Supported by:

    This research was supported by the National High Technology Research and Development Program of China (863 Program) (2011AA100501) and the National Natural Science Foundation of China (31461143024).

摘要:

小麦6-SFT是果聚糖合成的关键酶基因。23份六倍体普通小麦(AABBDD)5D基因组材料(DD)为多样性代表群体材料,通过测序分析小麦6-SFT-D基因的序列多态性,根据多态性开发6-SFT-D基因的功能标记,分析由154份六倍体普通小麦构成的自然群体的6-SFT-D基因单倍型(haplotype)与表型性状的关联特性基因累加效应。28份多样性代表群体中,共检测到6-SFT-D基因的4个多态性位点,均为单核苷酸多态性(SNP)位点,构成36-SFT-D基因单倍型;而在自然群体中只检测到6-SFT-D的两种单倍型。根据6-SFT-D基因2850 bp位点的T/C变异开发等位变异特异PCR标记。关联分析表明,6-SFT-D单倍型分别与灌溉条件下的千粒重和穗长显著关联,单倍型Hap I是提高千粒重的优异等位变异;在雨养和灌溉条件下,同时具有6-SFT-D6-SFT-A2优异等位变异小麦材料的千粒重显著高于其他基因型材料,说明6-SFT-D6-SFT-A2优异等位变异对于提高千粒重表现累加效应。

关键词: 普通小麦, 6-SFT-D, 功能标记, 单核苷酸多态性, 关联分析, 累加效应

Abstract:

Gene 6-SFT encodes a key enzyme in fructan biosynthesis pathway in common wheat (Triticum aestivum L.). In this study, we analyzed the single nucleotide polymorphism (SNP) on 6-SFT-D locus in a diversity population of 23 hexaploid wheat (AABBDD) accessions and five wheat relative species (DD) by means of direct sequencing. Functional markers were developed according to the sequence polymorphism. The correlation between 6-SFT-D haplotypes and phenotypic traits and the cumulative effect of 6-SFT alleles were analyzed using a natural population consisting of 154 historical wheat accessions. Four SNPs were detected on 6-SFT-D locus in the diversity population, forming three haplotypes. However, only two 6-SFT-D haplotypes were identified in the natural population. We developed a pair of allele-specific PCR markers based on a polymorphism (T/C) at the 2850 bp site. The results of haplotype–trait association analysis showed that 6-SFT-D was significantly associated with thousand-grain weight (TGW) and spike length under well-watered conditions. HapI was superior in improving TGW. Under drought stress and well-watered conditions, wheat materials carrying both 6-SFT-D and 6-SFT-A2 had significantly higher TGW than other genotypes, suggesting that 6-SFT-D and 6-SFT-A2 have cumulative effect on TGW improvement.

Key words: Wheat, 6-SFT-D, Functional marker, SNP, Association analysis, Cumulative effect

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