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作物学报 ›› 2016, Vol. 42 ›› Issue (07): 990-999.doi: 10.3724/SP.J.1006.2016.00990

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆盐胁迫相关GmNAC基因的鉴定、表达及变异分析

张彦威1,2,张礼凤1,李伟1,王彩洁1,张军1,徐冉1, *   

  1. 1 山东省农业科学院作物研究所, 山东济南 250131; 2 东北农业大学大豆生物学教育部重点实验室, 黑龙江哈尔滨 150001
  • 收稿日期:2015-12-16 修回日期:2016-03-14 出版日期:2016-07-12 网络出版日期:2016-03-28
  • 通讯作者: 徐冉, E-mail: soybeanxu@126.com
  • 基金资助:

    本研究由?东北农业大学大豆生物学教育部重点实验室开放基金项目(SB14A04), 国家现代农业产业技术体系建设专项(CARS-04-CES18), 国家自然科学基金项目(31501329)和山东省自然科学基金项目(ZR2015YL070)资助。

Identification, Expression and Variation Analysis of Salt Tolerance Related GmNAC Genes in Soybean

ZHANG Yan-Wei1, 2,ZHANG Li-Feng1,LI Wei1,WANG Cai-Jie1,ZHANG Jun1,XU Ran1, *   

  1. 1 Crop Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250131, China; 2 Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin 150001, China
  • Received:2015-12-16 Revised:2016-03-14 Published:2016-07-12 Published online:2016-03-28
  • Contact: 徐冉, E-mail: soybeanxu@126.com
  • Supported by:

    The work was supported by Open Foundation of Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University (SB14A04), China Agriculture Research System (CARS-04-CES18), National Natural Science Foundation of China (31501329), and Shandong Provincial Natural Science Foundation (ZR2015YL070).

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摘要：

NAC基因在植物的逆境胁迫中发挥着重要作用。本研究参照水稻和拟南芥的逆境相关NAC基因, 采用生物信息学方法鉴定了大豆逆境相关GmNAC基因, 利用荧光定量PCR技术分析了GmNAC基因在耐盐差异的大豆品种根部、叶片的表达及其对NaCl胁迫的应答, 采用反转录PCR技术克隆了表达差异显著的GmNAC基因。结果表明, 大豆GmNAC基因家族包含175个基因, 其中11个GmNAC蛋白与水稻和拟南芥的逆境相关NAC蛋白位于同一进化分支, 这些蛋白具有高度保守的NAC结构域; 这11个GmNAC基因在大豆根部的表达均高于在叶片, 而且在叶片和根部均受NaCl诱导, 部分基因在根部和叶片以及品种间表现出不同的表达规律; 在大豆品种齐黄34、徐豆10和汾豆95中, Glyma06g11970.1存在3个同义突变和1个非同义突变, Glyma06g16440.2存在1个同义突变。

关键词: 大豆, GmNAC, 进化树, NaCl处理, 表达分析, 序列变异

Abstract:

NAC genes play an important role in plant stress tolerance. In this study, bioinformatics method was used to identify the stress related GmNAC gene in soybean; the expression of candidated GmNAC genes in root and leaf was analyzed in soybean with NaCl treatment by Real-time-PCR. Reverse transcription PCR was performed to clone genes with significant difference in expression. The results showed that there were 175 genes in soybean GmNAC gene family. There were 11 GmNACproteins with highly conserved NAC located on the same evolutionary branch with the stress related NAC proteins in rice and Arabidopsis. The expression of 11 GmNAC genes in soybean root was higher than that in leaf. The GmNAC genes were all induced by NaCl stress, but part of the GmNAC genes showed different expression levels between root and leaf in soybean varieties with different salt tolerances. There were three synonymous mutations and one non-synonymous mutation on the CDS region of Glyma06g11970.1 and one synonymous mutation on the CDS region of Glyma06g16440.2 in Qihuang 34, Xudou 10, and Fendou 95.

Key words: Soybean, GmNAC, Phylogenetic tree, NaCl treatment, Expression analysis, Sequence variation

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