作物学报 ›› 2016, Vol. 42 ›› Issue (11): 1638-1646.
朱亚军1,**,孙强2,**,王金明2,陈凯1,冯博1,方雅洁1,林秀云2,*,徐建龙1,3,*
ZHU Ya-Jun1,**, SUN Qiang2,**, WANG Jin-Ming2, CHEN Kai1, FENG Bo1, FANG Ya-Jie1, LIN Xiu-Yun2,*, and XU Jian-Long1,3,*
摘要:
稻瘟病是水稻生产中危害最为严重的病害之一,种植抗病品种是抵御稻瘟病危害的有效措施。本研究利用吉粳809的2个亲本材料吉粳88与93072配制的回交分离群体进行稻瘟病人工接种,采用抗、感极端分池法定位双亲的稻瘟病抗性基因,结合基因型分析,推断吉粳809的抗性基因组成。结果表明,回交群体对强致病菌GD9-1表现为4个主效抗病基因Pi-2(t)、Pi-7-1(t)、Pi-7-2(t)和Pi-11(t)分离,对弱致病菌GD19-1表现单个主效抗病基因Pi-2(t)分离,其中Pi-2(t)基因同时抗2个菌株。除Pi-2(t)位点抗性等位基因来自吉粳88,其余3个位点的抗性等位基因均来自93072。比较基因组研究表明,Pi-2(t)可能与Pi-b等位,Pi-11(t)可能与Pi-47(t)或Pik等位,而Pi-7-1(t)和Pi-7-2(t)是2个新的抗性位点,分别与RM21260和RM8037连锁,遗传距离为0.11 cM和6.97 cM。利用与上述4个抗病位点紧密连锁的SSR标记和来自3K水稻种质资源重测序开发的55K芯片鉴定抗性位点吉粳809与双亲基因型的异同,推断吉粳809抗性基因组成,发现吉粳809携带轮回亲本吉粳88的Pi-2(t)和来自供体亲本93072的Pi-11(t) 2个基因,合理地解释了吉粳809抗性明显好于吉粳88的原因。对如何通过不同主效抗性基因聚合特别是充分利用原来抗病品种中“丧失”抗性残效基因来改良品种的稻瘟病抗性进行了讨论。
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