欢迎访问作物学报,今天是

作物学报 ›› 2017, Vol. 43 ›› Issue (12): 1774-1783.doi: 10.3724/SP.J.1006.2017.01774

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

黄淮海地区大豆主栽品种对8个大豆疫霉菌株的抗性评价

李晓那1,2,孙石2,**,钟超2,韩天富1,2*   

  1. 1东北农业大学农学院, 黑龙江哈尔滨 150030; 2中国农业科学院作物科学研究所 / 农业部北京大豆生物学重点实验室, 北京 100081
  • 收稿日期:2017-04-12 修回日期:2017-07-23 出版日期:2017-12-12 网络出版日期:2017-08-10
  • 通讯作者: 韩天富, E-mail: hantianfu@caas.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31471521), 国家现代农业产业技术体系建设专项(CARS-04), 国家公益性行业(农业)科研专项经费项目(201303018)和中国农业科学院农业科技创新工程资助。

Resistance Evaluation to Eight Phytophthora sojae Isolates for Major Soybean Cultivars in Huang-Huai-Hai Rivers Valley

LI Xiao-Na1,2, SUN Shi2,**, ZHONG Chao2,HAN Tian-Fu1,2,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China; 2 Key Laboratory of Soybean Biology (Beijing) / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2017-04-12 Revised:2017-07-23 Published:2017-12-12 Published online:2017-08-10
  • Contact: 韩天富, E-mail: hantianfu@caas.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31471521), the China Agriculture Research System (CARS-04), the Special Fund for Agro-Scientific Research in the Public Interest (201303018), and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.

摘要:

随着麦茬免耕栽培技术的推广应用,黄淮海地区麦后夏播大豆生产中疫霉根腐病呈加重趋势。了解该地区大豆主栽品种对疫霉根腐病的抗性和筛选抗病亲本对培育新的高产广适抗病品种具有重要意义。本研究利用8个具有不同毒力的大豆疫霉菌株,采用下胚轴创伤接种法,对20世纪50年代以来黄淮海地区审定、推广的140个大豆主栽品种接种鉴定表明,除6个品种无抗性外,其余134个品种分别抗1~8个大豆疫霉菌株,占鉴定品种总数的95.7%,其中抗6个以上菌株的品种有83个,占鉴定品种总数的59.3%。以14个鉴别寄主的抗病反应型为参照,发现134个品种对8个大豆疫霉菌株共产生65种反应型,其中19个品种产生的5种反应型与已知单基因或2个单基因组合反应型相同;115个品种产生的60种反应型与含有已知单基因或2个单基因组合的反应型不同,推测可能含有新的抗病基因或基因组合。根据研究结果合理选择亲本,可培育出聚合多个抗性基因且综合性状优良的大豆新品种。

关键词: 大豆, 疫霉根腐病, 大豆疫霉, 抗性基因, 基因推导

Abstract:

With the application of wheat stubble no-tillage cultivation technology, the occurrence rate of phytophthora root rot of soybean is getting higher in the Huang-Huai-Hai Rivers Valley. Therefore, it is crucial to investigate the resistance of the widely-grown soybean cultivars to phytophthora root rot and to screen disease-resistant resources for soybean improvement. In the current study, a total of 140 soybean cultivars, widely-grown in Huang-Huai-Hai Rivers Valley since the 1950s, were investigated for their response to eight strains of Phytophthora sojae through the hypocotyl inoculation method. Six cultivars were susceptible to all eight strains, and 134 or 95.7% of the identified cultivars were resistant to one to eight strains of Phytophthora sojae. Among the cultivars, 83 or 59.3% were resistant to more than six Phytophthora sojae strains, and showed 65 reaction types identified by comparing with the reaction types of 14 reference cultivars. Five reaction types produced by 19 cultivars were the same as those of the known single gene or two gene combinations, and 60 reaction types produced by 115 cultivars were different from those controlled by the known single gene or two gene combinations, suggesting that there were novel resistant genes or gene combinations. The research results are available for selecting parents to breed new elite soybean cultivars with pyramided of resistant genes and excellent agronomic performances.

Key words: Soybean, Phytophthora root rot, Phytophthora sojae, Resistance gene, Gene postulation

[1]王幸, 吴存祥, 齐玉军, 徐泽俊, 王宗标, 韩天富. 麦秸处理和播种方式对夏大豆农艺性状及土壤物理性状的影响. 中国农业科学, 2016, 49: 1453–1465 Wang X, Wu C X, Qi Y J, Xu Z J, Wang Z B, Han T F. Effects of straw management and sowing methods on soybean agronomic traits and soil physical properties. Sci Agric Sin, 2016, 49: 1453–1465 (in Chinese with English abstract) [2]罗永藩. 我国少耕与免耕技术推广应用情况与发展前景. 耕作与栽培, 1991, (2): 1–7 Luo Y F. The popularization and application of less tillage and no tillage technology in China. GENGZUO YU ZAIPEI, 1991, (2): 1–7 (in Chinese) [3]田慧, 谭周进, 屠乃美, 李建国, 肖启明. 少免耕土壤生态学效应研究进展. 耕作与栽培, 2006, (5): 10–12 Tian H, Tan Z J, Tu N M, Li J G, Xiao Q M. Research progress on soil ecological effects of no tillage. GENGZUO YU ZAIPEI, 2006, (5): 10–12 (in Chinese) [4]朱振东, 王晓鸣, 戴法超. 大豆疫霉根腐病在我国的发生及防治对策. 植物保护, 1999, 25: 47–49 Zhu Z D, Wang X M, Dai F C. Occurrence and control of phytophthora root rot of soybean in China. Plant Prot, 1999, 25: 47–49 (in Chinese) [5]Sun J T, Li L H, Zhao J M, Huang J, Yan Q, Xing H, Guo N. Genetic analysis and fine mapping of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max(L.) Merr.]. Theor Appl Genet, 2014, 127: 913–919 [6]Sugimoto T, Kato M, Yoshida S, Matsumoto I, Kobayashi T, Kaga A, Hajika M, Yamamoto R, Watanabe K, Aino M, Matoh T, Walker D R, Biggs A R, Ishimoto M. Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed Sci, 2012, 61: 511–522 [7]Weng C, Yu K, Anderson T R, Poysa V. Mapping genes conferring resistance to phytophthora root rot of soybean, Rps1a and Rps7. J Hered, 2001, 92: 442–446 [8]Burnham K D, Dorrance A E, Francis D M, Fioritto R J, Martin S K St. Rps8, a new locus in soybean for resistance to Phytophthora sojae. Crop Sci, 2003, 43: 101–105 [9]Gordon S G, Martin S K St, Dorrance A E. Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci, 2006, 46: 168–173 [10]Wu X L, Zhang B Q, Sun S, Zhao J M, Yang F, Guo N, Gai J Y, Xing H. Identification, genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean. Sci Agric Sin, 2011, 10: 1506–1511 [11]Zhang J Q, Xia C J, Duan C X, Sun S L, Wang X M, Wu X F, Zhu Z D. Identification and candidate gene analysis of a novel Phytophthora resistance gene Rps10 in a Chinese soybean cultivar. PLoS ONE, 2013, 8: e69799 [12]朱振东, 霍云龙, 王晓鸣, 黄俊斌, 武小菲. 一个抗大豆疫霉根腐病新基因的分子鉴定. 作物学报, 2007, 33: 154–157 Zhu Z D, Huo Y L, Wang X M, Huang J B, Wu X F. Molecular identification of a novel Phytophthora resistance gene in soybean. Acta Agron Sin, 2007, 33: 154–157 (in Chinese with English abstract) [13]Sun S, Wu X L, Zhao J M, Wang Y C, Tang Q H, Yu D Y, Gai J Y, Xing H. Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed, 2011, 130: 139–143 [14]范爱颖, 王晓鸣, 方小平, 武小菲, 朱振东. 大豆品种豫豆25抗疫霉根腐病基因的鉴定. 作物学报, 2009, 35: 1844–1850 Fan A Y, Wang X M, Fang X P, Wu X F, Zhu Z D. Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou25. Acta Agron Sin, 2009, 35: 1844–1850 (in Chinese with English abstract) [15]姚海燕, 王晓鸣, 武小菲, 肖炎农, 朱振东. 大豆品种早熟18抗疫霉根腐病基因的SSR分子标记. 植物遗传资源学报, 2010, 11: 213–217 Yao H Y, Wang X M, Wu X F, Xiao Y N, Zhu Z D. Molecular mapping of Phytophthora resistance gene in soybean cultivar Zaoshu18. J Plant Genet Resour, 2010, 11: 213–217 (in Chinese with English abstract) [16]于安亮, 徐鹏飞, 王金生, 张淑珍, 吴俊江, 李文滨, 陈维元, 李宁辉, 范素杰, 王欣, 姜良宇. 大豆品种绥农10抗疫霉根腐病遗传分析及抗病基因的SSR标记. 中国油料作物学报, 2010, 32: 462–466 Yu A L, Xu P F, Wang J S, Zhang S Z, Wu J J, Li W B, Chen W Y, Li N H, Fan S J, Wang X, Jiang L Y. Genetic analysis and SSR mapping of gene resistance to Phytophthora sojae race 1 in soybean cv Suinong10. Chin J Oil Crop Sci, 2010, 32: 462–466 (in Chinese with English abstract) [17]武晓玲, 周斌, 孙石, 赵晋铭, 陈受宜, 盖钧镒, 邢邯. 大豆对大豆疫霉菌株Pm14抗性的遗传分析及基因定位. 中国农业科学, 2011, 44: 456–460 Wu X L, Zhou B, Sun S, Zhao J M, Chen S Y, Gai J Y, Xing H. Genetic analysis and mapping of resistance to Phytophthora sojae of Pm14 in soybean. Sci Agric Sin, 2011, 44: 456–460 (in Chinese with English abstract) [18]Zhang J Q, Xia C J, Wang X M, Duan C X, Sun S L, Wu X F, Zhu Z D. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor Appl Genet, 2013, 126: 1555–1561 [19]Lin F, Zhao M X, Ping J Q, Johnson A, Zhang B, Abney T S, Hughes T J, Ma J X. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor Appl Genet, 2013, 126: 2177–2185 [20]Ping J Q, Fitzgerald J C, Zhang C B, Lin F, Bai Y H, Wang D C, Aggarwal R, Rehman M, Crasta O, Ma J X. Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean. Theor Appl Genet, 2016, 129: 445–451 [21]Sahoo D K, Abeysekara N S, Cianzio S R, Robertson A E, Bhattacharyya M K. A novel Phytophthora sojae resistance Rps12 gene mapped to a genomic region that contains several Rps genes, PLoS ONE, 2017, 12: e0169950 [22]Li Y P, Sun S L, Zhong C, Wang X M, Wu X F, Zhu Z D. Genetic mapping and development of co-segregating markers of RpsQ, which provides resistance to Phytophthora sojae in soybean. Theor Appl Genet, 2017, doi: 10.1007/s00122-017-2883-7 [23]Cheng Y B, Ma Q B, Ren H L, Xia Q J, Song E L, Tan Z Y, Li S X, Zhang G Y, Nian H. Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing. Theor Appl Genet, 2017, 130: 1041–1051 [24]Schmitthenner A F. Problems and progress in control of phytophthora root rot of soybean. Plant Dis, 1985, 69: 362–368 [25]Yang B, Wang Q Q, Jing M F, Guo B D, Wu J W, Wang H N, Wang Y, Lin L, Wang Y, Ye W W, Dong S M, Wang Y C. Distinct regions of the Phytophthora essential effector Avh238 determine its function in cell death activation and plant immunity suppression. New Phytol, 2017, 214: 361–375 [26]Lee S, Mian M A R, Sneller C H, Wang H H, Dorrance A E, McHale L K. Joint linkage QTL analyses for partial resistance to Phytophthora sojae in soybean using six nested inbred populations with heterogeneous conditions. Theor Appl Genet, 2014, 127: 429–444 [27]朱振东, 王化波, 王晓鸣, 武小菲. 黑龙江省主要栽培大豆品种(系)对大豆疫霉根腐病的多抗性评价. 植物遗传资源学报, 2004, 5: 22–25 Zhu Z D, Wang H B, Wang X M, Wu X F. Response of soybean cultivars or lines developed in Heilongjiang province to five strains of Phytophthora sojae. J Plant Genet Resour, 2004, 5: 22–25 (in Chinese with English abstract) [28]朱振东, 王晓鸣, 常汝镇, 马淑梅, 武小菲, 田玉兰. 黑龙江省大豆疫霉菌生理小种鉴定及大豆种质的抗性评价. 中国农业科学, 2000, 33: 62–67 Zhu Z D, Wang X M, Chang R Z, Ma S M, Wu X F, Tian Y L. Identification of race of Phytophthora sojae and reaction of soybean germplasm resources in Heilongjiang Province. Sci Agric Sin, 2000, 33: 62–67 (in Chinese with English abstract) [29]Kyle D E, Nickell C D, Nelson R L, Pedersen W L. Response of soybean accessions from provinces in southern China to Phytophthora sojae. Plant Dis, 1998, 82: 555–559 [30]孙石, 赵晋铭, 武晓玲, 郭娜, 王源超, 盖钧镒, 邢邯. 黄淮海地区大豆种质对疫霉根腐病的抗性分析. 大豆科学, 2008, 27: 465–470 Sun S, Zhao J M, Wu X L, Guo N, Wang Y C, Gai J Y, Xing H. Resistance of soybean gemplasm to phytophthora in Huanghuai Valley. Soybean Sci, 2008, 27: 465–470 (in Chinese with English abstract) [31]Lohnes D G, Nickell C D, Schmitthenner A F. Origin of soybean alleles for Phytophthora resistance in China. Crop Sci, 1996, 36: 1689–1692 [32]朱振东, 霍云龙, 王晓鸣, 黄俊斌, 武小菲. 大豆疫霉根腐病抗源筛选. 植物遗传资源学报, 2006, 7: 24–30 Zhu Z D, Huo Y L, Wang X M, Huang J B, Wu X F. Screening for resistance sources to phytophthora root rot in soybean. J Plant Genet Resour, 2006, 7: 24–30 (in Chinese with English abstract) [33]任海龙, 宋恩亮, 马启彬, 杨存义, 王瑞鹏, 马天翔, 唐玉娟, 年海. 南方三省(区)抗大豆疫霉根腐病野生大豆资源的筛选. 大豆科学, 2010, 29: 1012–1015 Ren H L, Song E L, Ma Q B, Yang C Y, Wang R P, Ma T X, Tang Y J, Nian H. Screening for resistance sources to phytophthora root rot in glycine sojae from three provinces of Southern China. Soybean Sci, 2010, 29: 1012–1015 (in Chinese with English abstract) [34]程艳波, 马启彬, 牟英辉, 谭志远, 吴鸿, 年海. 华南地区推广应用大豆品种对疫霉根腐病的抗性评价. 华南农业大学学报, 2015, 36: 69–75 Cheng Y B, Ma Q B, Mu Y H, Tan Z Y, Wu H, Nian H. Resistance evaluation of soybean varieties to phytophthora root rot in South China. Huanan J Agric Sci, 2015, 36: 69–75 (in Chinese with English abstract) [35]申宏波, 丁俊杰, 于永梅, 姚文秋, 顾鑫, 杨晓贺, 郑天琪, 赵海红. 2009年黑龙江省大豆新品系抗疫霉根腐病鉴定与评价. 大豆科学, 2010, 29: 1087–1090 Shen H B, Ding J J, Yu Y M, Yao W Q, Gu X, Yang X H, Zheng T Q, Zhao H H. Identification and evaluation on soybean lines resistant to phytophthora root rot from Heilongjiang province in 2009. Soybean Sci, 2010, 29: 1087–1090 (in Chinese with English abstract) [36]徐鹏飞, 姜良宇, 李文滨, 张淑珍, 陈维元, 吴俊江, 李岑, 邱丽娟, 常汝镇, 王金生. 黑龙江省大豆品种对大豆疫霉根腐病抗性评价及抗性基因推导. 中国油料作物学报, 2011, 33: 521–526 Xu P F, Jiang L Y, Li W B, Zhang S Z, Chen W Y, Wu J J, Li C, Qiu L J, Chang R Z, Wang J S. Screening on soybean cultivars resistance to Phytophthora sojae and genes postulation in Heilongjiang Province. Chin J Oil Crop Sci, 2011, 33: 521–526 (in Chinese with English abstract) [37]唐庆华, 崔林开, 李德龙, 戴婷婷, 阴伟晓, 董莎萌, 邢邯, 郑小波, 王源超. 黄淮地区大豆种质资源对疫霉根腐病的抗病性评价. 中国农业科学, 2010, 43: 2246–2252 Tang Q H, Cui L K, Li D L, Dai T T, Yin W X, Dong S M, Xing H, Zheng X B, Wang Y C. Resistance evaluation of soybean germplasm from Huanghuai valley to phytophthora root rot. Sci Agri Sin, 2010, 43: 2246–2252 (in Chinese with English abstract)

[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[3] 王炫栋, 杨孙玉悦, 高润杰, 余俊杰, 郑丹沛, 倪峰, 蒋冬花. 拮抗大豆斑疹病菌放线菌菌株的筛选和促生作用及防效研究[J]. 作物学报, 2022, 48(6): 1546-1557.
[4] 邓钊, 江南, 符辰建, 严天泽, 符星学, 胡小淳, 秦鹏, 刘珊珊, 王凯, 杨远柱. 隆两优与晶两优系列杂交稻的稻瘟病抗性基因分析[J]. 作物学报, 2022, 48(5): 1071-1080.
[5] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[6] 李阿立, 冯雅楠, 李萍, 张东升, 宗毓铮, 林文, 郝兴宇. 大豆叶片响应CO2浓度升高、干旱及其交互作用的转录组分析[J]. 作物学报, 2022, 48(5): 1103-1118.
[7] 彭西红, 陈平, 杜青, 杨雪丽, 任俊波, 郑本川, 罗凯, 谢琛, 雷鹿, 雍太文, 杨文钰. 减量施氮对带状套作大豆土壤通气环境及结瘤固氮的影响[J]. 作物学报, 2022, 48(5): 1199-1209.
[8] 王好让, 张勇, 于春淼, 董全中, 李微微, 胡凯凤, 张明明, 薛红, 杨梦平, 宋继玲, 王磊, 杨兴勇, 邱丽娟. 大豆突变体ygl2黄绿叶基因的精细定位[J]. 作物学报, 2022, 48(4): 791-800.
[9] 李瑞东, 尹阳阳, 宋雯雯, 武婷婷, 孙石, 韩天富, 徐彩龙, 吴存祥, 胡水秀. 增密对不同分枝类型大豆品种同化物积累和产量的影响[J]. 作物学报, 2022, 48(4): 942-951.
[10] 杜浩, 程玉汉, 李泰, 侯智红, 黎永力, 南海洋, 董利东, 刘宝辉, 程群. 利用Ln位点进行分子设计提高大豆单荚粒数[J]. 作物学报, 2022, 48(3): 565-571.
[11] 周悦, 赵志华, 张宏宁, 孔佑宾. 大豆紫色酸性磷酸酶基因GmPAP14启动子克隆与功能分析[J]. 作物学报, 2022, 48(3): 590-596.
[12] 王娟, 张彦威, 焦铸锦, 刘盼盼, 常玮. 利用PyBSASeq算法挖掘大豆百粒重相关位点与候选基因[J]. 作物学报, 2022, 48(3): 635-643.
[13] 董衍坤, 黄定全, 高震, 陈栩. 大豆PIN-Like (PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能[J]. 作物学报, 2022, 48(2): 353-366.
[14] 张国伟, 李凯, 李思嘉, 王晓婧, 杨长琴, 刘瑞显. 减库对大豆叶片碳代谢的影响[J]. 作物学报, 2022, 48(2): 529-537.
[15] 宋丽君, 聂晓玉, 何磊磊, 蒯婕, 杨华, 郭安国, 黄俊生, 傅廷栋, 汪波, 周广生. 饲用大豆品种耐荫性鉴定指标筛选及综合评价[J]. 作物学报, 2021, 47(9): 1741-1752.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!