作物学报 ›› 2017, Vol. 43 ›› Issue (12): 1774-1783.doi: 10.3724/SP.J.1006.2017.01774
李晓那1,2,孙石2,**,钟超2,韩天富1,2*
LI Xiao-Na1,2, SUN Shi2,**, ZHONG Chao2,HAN Tian-Fu1,2,*
摘要:
随着麦茬免耕栽培技术的推广应用,黄淮海地区麦后夏播大豆生产中疫霉根腐病呈加重趋势。了解该地区大豆主栽品种对疫霉根腐病的抗性和筛选抗病亲本对培育新的高产广适抗病品种具有重要意义。本研究利用8个具有不同毒力的大豆疫霉菌株,采用下胚轴创伤接种法,对20世纪50年代以来黄淮海地区审定、推广的140个大豆主栽品种接种鉴定表明,除6个品种无抗性外,其余134个品种分别抗1~8个大豆疫霉菌株,占鉴定品种总数的95.7%,其中抗6个以上菌株的品种有83个,占鉴定品种总数的59.3%。以14个鉴别寄主的抗病反应型为参照,发现134个品种对8个大豆疫霉菌株共产生65种反应型,其中19个品种产生的5种反应型与已知单基因或2个单基因组合反应型相同;115个品种产生的60种反应型与含有已知单基因或2个单基因组合的反应型不同,推测可能含有新的抗病基因或基因组合。根据研究结果合理选择亲本,可培育出聚合多个抗性基因且综合性状优良的大豆新品种。
[1]王幸, 吴存祥, 齐玉军, 徐泽俊, 王宗标, 韩天富. 麦秸处理和播种方式对夏大豆农艺性状及土壤物理性状的影响. 中国农业科学, 2016, 49: 1453–1465 Wang X, Wu C X, Qi Y J, Xu Z J, Wang Z B, Han T F. Effects of straw management and sowing methods on soybean agronomic traits and soil physical properties. Sci Agric Sin, 2016, 49: 1453–1465 (in Chinese with English abstract) [2]罗永藩. 我国少耕与免耕技术推广应用情况与发展前景. 耕作与栽培, 1991, (2): 1–7 Luo Y F. The popularization and application of less tillage and no tillage technology in China. GENGZUO YU ZAIPEI, 1991, (2): 1–7 (in Chinese) [3]田慧, 谭周进, 屠乃美, 李建国, 肖启明. 少免耕土壤生态学效应研究进展. 耕作与栽培, 2006, (5): 10–12 Tian H, Tan Z J, Tu N M, Li J G, Xiao Q M. Research progress on soil ecological effects of no tillage. GENGZUO YU ZAIPEI, 2006, (5): 10–12 (in Chinese) [4]朱振东, 王晓鸣, 戴法超. 大豆疫霉根腐病在我国的发生及防治对策. 植物保护, 1999, 25: 47–49 Zhu Z D, Wang X M, Dai F C. Occurrence and control of phytophthora root rot of soybean in China. Plant Prot, 1999, 25: 47–49 (in Chinese) [5]Sun J T, Li L H, Zhao J M, Huang J, Yan Q, Xing H, Guo N. Genetic analysis and fine mapping of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max(L.) Merr.]. Theor Appl Genet, 2014, 127: 913–919 [6]Sugimoto T, Kato M, Yoshida S, Matsumoto I, Kobayashi T, Kaga A, Hajika M, Yamamoto R, Watanabe K, Aino M, Matoh T, Walker D R, Biggs A R, Ishimoto M. Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed Sci, 2012, 61: 511–522 [7]Weng C, Yu K, Anderson T R, Poysa V. Mapping genes conferring resistance to phytophthora root rot of soybean, Rps1a and Rps7. J Hered, 2001, 92: 442–446 [8]Burnham K D, Dorrance A E, Francis D M, Fioritto R J, Martin S K St. Rps8, a new locus in soybean for resistance to Phytophthora sojae. Crop Sci, 2003, 43: 101–105 [9]Gordon S G, Martin S K St, Dorrance A E. Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci, 2006, 46: 168–173 [10]Wu X L, Zhang B Q, Sun S, Zhao J M, Yang F, Guo N, Gai J Y, Xing H. Identification, genetic analysis and mapping of resistance to Phytophthora sojae of Pm28 in soybean. Sci Agric Sin, 2011, 10: 1506–1511 [11]Zhang J Q, Xia C J, Duan C X, Sun S L, Wang X M, Wu X F, Zhu Z D. Identification and candidate gene analysis of a novel Phytophthora resistance gene Rps10 in a Chinese soybean cultivar. PLoS ONE, 2013, 8: e69799 [12]朱振东, 霍云龙, 王晓鸣, 黄俊斌, 武小菲. 一个抗大豆疫霉根腐病新基因的分子鉴定. 作物学报, 2007, 33: 154–157 Zhu Z D, Huo Y L, Wang X M, Huang J B, Wu X F. Molecular identification of a novel Phytophthora resistance gene in soybean. Acta Agron Sin, 2007, 33: 154–157 (in Chinese with English abstract) [13]Sun S, Wu X L, Zhao J M, Wang Y C, Tang Q H, Yu D Y, Gai J Y, Xing H. Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed, 2011, 130: 139–143 [14]范爱颖, 王晓鸣, 方小平, 武小菲, 朱振东. 大豆品种豫豆25抗疫霉根腐病基因的鉴定. 作物学报, 2009, 35: 1844–1850 Fan A Y, Wang X M, Fang X P, Wu X F, Zhu Z D. Molecular identification of Phytophthora resistance gene in soybean cultivar Yudou25. Acta Agron Sin, 2009, 35: 1844–1850 (in Chinese with English abstract) [15]姚海燕, 王晓鸣, 武小菲, 肖炎农, 朱振东. 大豆品种早熟18抗疫霉根腐病基因的SSR分子标记. 植物遗传资源学报, 2010, 11: 213–217 Yao H Y, Wang X M, Wu X F, Xiao Y N, Zhu Z D. Molecular mapping of Phytophthora resistance gene in soybean cultivar Zaoshu18. J Plant Genet Resour, 2010, 11: 213–217 (in Chinese with English abstract) [16]于安亮, 徐鹏飞, 王金生, 张淑珍, 吴俊江, 李文滨, 陈维元, 李宁辉, 范素杰, 王欣, 姜良宇. 大豆品种绥农10抗疫霉根腐病遗传分析及抗病基因的SSR标记. 中国油料作物学报, 2010, 32: 462–466 Yu A L, Xu P F, Wang J S, Zhang S Z, Wu J J, Li W B, Chen W Y, Li N H, Fan S J, Wang X, Jiang L Y. Genetic analysis and SSR mapping of gene resistance to Phytophthora sojae race 1 in soybean cv Suinong10. Chin J Oil Crop Sci, 2010, 32: 462–466 (in Chinese with English abstract) [17]武晓玲, 周斌, 孙石, 赵晋铭, 陈受宜, 盖钧镒, 邢邯. 大豆对大豆疫霉菌株Pm14抗性的遗传分析及基因定位. 中国农业科学, 2011, 44: 456–460 Wu X L, Zhou B, Sun S, Zhao J M, Chen S Y, Gai J Y, Xing H. Genetic analysis and mapping of resistance to Phytophthora sojae of Pm14 in soybean. Sci Agric Sin, 2011, 44: 456–460 (in Chinese with English abstract) [18]Zhang J Q, Xia C J, Wang X M, Duan C X, Sun S L, Wu X F, Zhu Z D. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor Appl Genet, 2013, 126: 1555–1561 [19]Lin F, Zhao M X, Ping J Q, Johnson A, Zhang B, Abney T S, Hughes T J, Ma J X. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor Appl Genet, 2013, 126: 2177–2185 [20]Ping J Q, Fitzgerald J C, Zhang C B, Lin F, Bai Y H, Wang D C, Aggarwal R, Rehman M, Crasta O, Ma J X. Identification and molecular mapping of Rps11, a novel gene conferring resistance to Phytophthora sojae in soybean. Theor Appl Genet, 2016, 129: 445–451 [21]Sahoo D K, Abeysekara N S, Cianzio S R, Robertson A E, Bhattacharyya M K. A novel Phytophthora sojae resistance Rps12 gene mapped to a genomic region that contains several Rps genes, PLoS ONE, 2017, 12: e0169950 [22]Li Y P, Sun S L, Zhong C, Wang X M, Wu X F, Zhu Z D. Genetic mapping and development of co-segregating markers of RpsQ, which provides resistance to Phytophthora sojae in soybean. Theor Appl Genet, 2017, doi: 10.1007/s00122-017-2883-7 [23]Cheng Y B, Ma Q B, Ren H L, Xia Q J, Song E L, Tan Z Y, Li S X, Zhang G Y, Nian H. Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing. Theor Appl Genet, 2017, 130: 1041–1051 [24]Schmitthenner A F. Problems and progress in control of phytophthora root rot of soybean. Plant Dis, 1985, 69: 362–368 [25]Yang B, Wang Q Q, Jing M F, Guo B D, Wu J W, Wang H N, Wang Y, Lin L, Wang Y, Ye W W, Dong S M, Wang Y C. Distinct regions of the Phytophthora essential effector Avh238 determine its function in cell death activation and plant immunity suppression. New Phytol, 2017, 214: 361–375 [26]Lee S, Mian M A R, Sneller C H, Wang H H, Dorrance A E, McHale L K. Joint linkage QTL analyses for partial resistance to Phytophthora sojae in soybean using six nested inbred populations with heterogeneous conditions. Theor Appl Genet, 2014, 127: 429–444 [27]朱振东, 王化波, 王晓鸣, 武小菲. 黑龙江省主要栽培大豆品种(系)对大豆疫霉根腐病的多抗性评价. 植物遗传资源学报, 2004, 5: 22–25 Zhu Z D, Wang H B, Wang X M, Wu X F. Response of soybean cultivars or lines developed in Heilongjiang province to five strains of Phytophthora sojae. J Plant Genet Resour, 2004, 5: 22–25 (in Chinese with English abstract) [28]朱振东, 王晓鸣, 常汝镇, 马淑梅, 武小菲, 田玉兰. 黑龙江省大豆疫霉菌生理小种鉴定及大豆种质的抗性评价. 中国农业科学, 2000, 33: 62–67 Zhu Z D, Wang X M, Chang R Z, Ma S M, Wu X F, Tian Y L. Identification of race of Phytophthora sojae and reaction of soybean germplasm resources in Heilongjiang Province. Sci Agric Sin, 2000, 33: 62–67 (in Chinese with English abstract) [29]Kyle D E, Nickell C D, Nelson R L, Pedersen W L. Response of soybean accessions from provinces in southern China to Phytophthora sojae. Plant Dis, 1998, 82: 555–559 [30]孙石, 赵晋铭, 武晓玲, 郭娜, 王源超, 盖钧镒, 邢邯. 黄淮海地区大豆种质对疫霉根腐病的抗性分析. 大豆科学, 2008, 27: 465–470 Sun S, Zhao J M, Wu X L, Guo N, Wang Y C, Gai J Y, Xing H. Resistance of soybean gemplasm to phytophthora in Huanghuai Valley. Soybean Sci, 2008, 27: 465–470 (in Chinese with English abstract) [31]Lohnes D G, Nickell C D, Schmitthenner A F. Origin of soybean alleles for Phytophthora resistance in China. Crop Sci, 1996, 36: 1689–1692 [32]朱振东, 霍云龙, 王晓鸣, 黄俊斌, 武小菲. 大豆疫霉根腐病抗源筛选. 植物遗传资源学报, 2006, 7: 24–30 Zhu Z D, Huo Y L, Wang X M, Huang J B, Wu X F. Screening for resistance sources to phytophthora root rot in soybean. J Plant Genet Resour, 2006, 7: 24–30 (in Chinese with English abstract) [33]任海龙, 宋恩亮, 马启彬, 杨存义, 王瑞鹏, 马天翔, 唐玉娟, 年海. 南方三省(区)抗大豆疫霉根腐病野生大豆资源的筛选. 大豆科学, 2010, 29: 1012–1015 Ren H L, Song E L, Ma Q B, Yang C Y, Wang R P, Ma T X, Tang Y J, Nian H. Screening for resistance sources to phytophthora root rot in glycine sojae from three provinces of Southern China. Soybean Sci, 2010, 29: 1012–1015 (in Chinese with English abstract) [34]程艳波, 马启彬, 牟英辉, 谭志远, 吴鸿, 年海. 华南地区推广应用大豆品种对疫霉根腐病的抗性评价. 华南农业大学学报, 2015, 36: 69–75 Cheng Y B, Ma Q B, Mu Y H, Tan Z Y, Wu H, Nian H. Resistance evaluation of soybean varieties to phytophthora root rot in South China. Huanan J Agric Sci, 2015, 36: 69–75 (in Chinese with English abstract) [35]申宏波, 丁俊杰, 于永梅, 姚文秋, 顾鑫, 杨晓贺, 郑天琪, 赵海红. 2009年黑龙江省大豆新品系抗疫霉根腐病鉴定与评价. 大豆科学, 2010, 29: 1087–1090 Shen H B, Ding J J, Yu Y M, Yao W Q, Gu X, Yang X H, Zheng T Q, Zhao H H. Identification and evaluation on soybean lines resistant to phytophthora root rot from Heilongjiang province in 2009. Soybean Sci, 2010, 29: 1087–1090 (in Chinese with English abstract) [36]徐鹏飞, 姜良宇, 李文滨, 张淑珍, 陈维元, 吴俊江, 李岑, 邱丽娟, 常汝镇, 王金生. 黑龙江省大豆品种对大豆疫霉根腐病抗性评价及抗性基因推导. 中国油料作物学报, 2011, 33: 521–526 Xu P F, Jiang L Y, Li W B, Zhang S Z, Chen W Y, Wu J J, Li C, Qiu L J, Chang R Z, Wang J S. Screening on soybean cultivars resistance to Phytophthora sojae and genes postulation in Heilongjiang Province. Chin J Oil Crop Sci, 2011, 33: 521–526 (in Chinese with English abstract) [37]唐庆华, 崔林开, 李德龙, 戴婷婷, 阴伟晓, 董莎萌, 邢邯, 郑小波, 王源超. 黄淮地区大豆种质资源对疫霉根腐病的抗病性评价. 中国农业科学, 2010, 43: 2246–2252 Tang Q H, Cui L K, Li D L, Dai T T, Yin W X, Dong S M, Xing H, Zheng X B, Wang Y C. Resistance evaluation of soybean germplasm from Huanghuai valley to phytophthora root rot. Sci Agri Sin, 2010, 43: 2246–2252 (in Chinese with English abstract) |
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