不同种植方式大豆根际土壤细菌多样性分析

doi:10.3724/SP.J.1006.2018.01539

摘要/Abstract

摘要：

利用Illumina MiSeq第二代高通量测序平台, 对黑龙江省不同地区轮作及连作大豆根际土壤细菌16S rDNA基因组测序, 初步分析在不同种植方式下受大豆胞囊线虫侵染的大豆根际土壤细菌群落结构的变化。从6个土壤样本中共获得25 419个OTUs, 鉴定到细菌的47个门, 147个纲, 709个属。变形菌门、放线菌门、酸杆菌门、芽单胞菌门、浮霉菌门是供试土壤细菌的优势菌门, 占所有细菌群落总数90%以上。连作4年总OTUs及丰富度最高, 连作20年最低。不同轮作方式土壤细菌丰富度差异不显著(P > 0.05), 短期连作与长期连作细菌丰富度及多样性差异显著(P < 0.05)。不同轮作方式下放线菌门相对丰度低于连作方式, 芽单胞菌门和拟杆菌门相对丰度高于同地区连作方式。土壤功能细菌根瘤菌(Bradyrhizobium)、链霉菌属(Streptomyces)、芽孢杆菌属(Bacillus)、溶杆菌属(Lysobacter)、土微菌属(Pedomicrobium)的相对丰度在不同年限连作下高于轮作。长期连作土壤优势细菌丰度与轮作土壤相似性更高。

关键词: 大豆, 轮作, 连作, 细菌多样性, Illumina MiSeq 测序

Abstract:

To study bacterial community structure of soybean rhizosphere soil in rotation and continuous cropping, 16S rDNA gene were sequenced of soil samples infected soybean cyst nematode from Heilongjiang Province two regions by the second generation of high-throughput sequencing Illumina MiSeq platform. A total of 25 419 OTUs were obtained from six soil samples and classified into 47 phylum, 147 class, and 709 genera. Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes were dominant bacteria, accounting for more than 90% of all soil bacterial communities. The total OTUs and richness were the highest in four years continuous cropping and the lowest in twenty years continuous cropping. The difference of bacteria abundance in different rotational cropping years was not obvious (P > 0.05), but abundance and adversity were significant in continuous cropping (P < 0.05). The relative abundance of Actinobacteria in different rotations was lower than that in continuous cropping, and the relative abundance of Gemmatimonadetes and Bacteroidetes was higher than that in the same area. The relative abundance of soil functional bacteria Bradyrhizobium, Streptomyces, Bacillus, Lysobacter, and Pedomicrobium were higher in different continuous cropping years than those in rotations. Predominant bacterial abundance in long term continuous cropping was more similar with that in rotational cropping.

Key words: soybean, rotation, continuous cropping, bacterial diversity, Illumina MiSeq sequencing

王芳,陈井生,刘大伟. 不同种植方式大豆根际土壤细菌多样性分析[J]. 作物学报, 2018, 44(10): 1539-1547.

Fang WANG,Jing-Sheng CHEN,Da-Wei LIU. Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns[J]. Acta Agronomica Sinica, 2018, 44(10): 1539-1547.

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样品 Sample	有效序列数量 Effective sequence-number	OTUs数量 OTUs number (97%)	Chao指数 Chao index	Shannon指数 Shannon index
感病品种-大麻-抗病品种(大庆) RR (Daqing)	39530	4243	5247.7 a	10.45 a
感病品种-大麻-感病品种(大庆) RS (Daqing)	37614	4140	5122.7 a	10.57 a
玉米-谷子-感病品种(齐齐哈尔) RQ (Qiqihar)	54387	4229	4972.7 a	10.20 b

样品 Sample	有效序列数量 Effective sequence-number	OTUs数量 OTUs number (97%)	Chao指数 Chao index	Shannon指数 Shannon index
连作4年(大庆) C4 (Daqing)	67271	4928	5659.6 a	10.47 a
连作10年(大庆) C10 (Daqing)	69356	4173	4583.8 b	10.23 b
连作20年(齐齐哈尔) C20 (Qiqihar )	52387	3706	4147.0 b	10.09 c