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作物学报 ›› 2018, Vol. 44 ›› Issue (10): 1539-1547.doi: 10.3724/SP.J.1006.2018.01539

• 耕作栽培·生理生化 • 上一篇    下一篇

不同种植方式大豆根际土壤细菌多样性分析

王芳1,陈井生2,刘大伟3   

  1. 1齐齐哈尔大学生命科学与农林学院 / 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江齐齐哈尔 161006
    2黑龙江省农科院大庆分院, 黑龙江大庆 163316
    3东北农业大学农学院, 黑龙江哈尔滨 150030
  • 收稿日期:2018-02-05 接受日期:2018-07-20 出版日期:2018-10-10 网络出版日期:2018-07-31
  • 基金资助:
    本研究由黑龙江省省属高等学校基本科研业务费科研项目(2012K-M21)

Bacterial Diversity of Soybean Rhizosphere Soil under Different Cropping Patterns

Fang WANG1,Jing-Sheng CHEN2,Da-Wei LIU3   

  1. 1 College of Life Sciences and Agroforestry, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University, Qiqihar 161006, Heilongjiang, China
    2 Daqing Branch of Heilongjiang Academy of Agricultural Sciences, Daqing 163316, Heilongjiang, China
    3 Agronomy College, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
  • Received:2018-02-05 Accepted:2018-07-20 Published:2018-10-10 Published online:2018-07-31
  • Supported by:
    This study was supported by the Heilongjiang Provincial Higher-education Basic Scientific Research Project(2012K-M21)

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摘要：

利用Illumina MiSeq第二代高通量测序平台, 对黑龙江省不同地区轮作及连作大豆根际土壤细菌16S rDNA基因组测序, 初步分析在不同种植方式下受大豆胞囊线虫侵染的大豆根际土壤细菌群落结构的变化。从6个土壤样本中共获得25 419个OTUs, 鉴定到细菌的47个门, 147个纲, 709个属。变形菌门、放线菌门、酸杆菌门、芽单胞菌门、浮霉菌门是供试土壤细菌的优势菌门, 占所有细菌群落总数90%以上。连作4年总OTUs及丰富度最高, 连作20年最低。不同轮作方式土壤细菌丰富度差异不显著(P > 0.05), 短期连作与长期连作细菌丰富度及多样性差异显著(P < 0.05)。不同轮作方式下放线菌门相对丰度低于连作方式, 芽单胞菌门和拟杆菌门相对丰度高于同地区连作方式。土壤功能细菌根瘤菌(Bradyrhizobium)、链霉菌属(Streptomyces)、芽孢杆菌属(Bacillus)、溶杆菌属(Lysobacter)、土微菌属(Pedomicrobium)的相对丰度在不同年限连作下高于轮作。长期连作土壤优势细菌丰度与轮作土壤相似性更高。

关键词: 大豆, 轮作, 连作, 细菌多样性, Illumina MiSeq 测序

Abstract:

To study bacterial community structure of soybean rhizosphere soil in rotation and continuous cropping, 16S rDNA gene were sequenced of soil samples infected soybean cyst nematode from Heilongjiang Province two regions by the second generation of high-throughput sequencing Illumina MiSeq platform. A total of 25 419 OTUs were obtained from six soil samples and classified into 47 phylum, 147 class, and 709 genera. Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, Planctomycetes were dominant bacteria, accounting for more than 90% of all soil bacterial communities. The total OTUs and richness were the highest in four years continuous cropping and the lowest in twenty years continuous cropping. The difference of bacteria abundance in different rotational cropping years was not obvious (P > 0.05), but abundance and adversity were significant in continuous cropping (P < 0.05). The relative abundance of Actinobacteria in different rotations was lower than that in continuous cropping, and the relative abundance of Gemmatimonadetes and Bacteroidetes was higher than that in the same area. The relative abundance of soil functional bacteria Bradyrhizobium, Streptomyces, Bacillus, Lysobacter, and Pedomicrobium were higher in different continuous cropping years than those in rotations. Predominant bacterial abundance in long term continuous cropping was more similar with that in rotational cropping.

Key words: soybean, rotation, continuous cropping, bacterial diversity, Illumina MiSeq sequencing

图1

相似度为97%水平下各样品稀释性曲线 RR: 感病品种-大麻-抗病品种; RS: 感病品种-大麻-感病品种; RQ: 玉米-谷子-感病品种; C4: 连作4年; C10: 连作10年; C20: 连作20年。"

图2

6个土壤样品丰度分布曲线 RR: 感病品种-大麻-抗病品种; RS: 感病品种-大麻-感病品种; RQ: 玉米-谷子-感病品种; C4: 连作4年; C10: 连作10年;C20: 连作20年。"

表1

不同轮作下大豆根际土壤细菌序列统计及多样性指数"

样品
Sample		 有效序列数量
Effective
sequence-number		 OTUs数量
OTUs number (97%)		 Chao指数
Chao index		 Shannon指数
Shannon index
感病品种-大麻-抗病品种(大庆) RR (Daqing) 39530 4243 5247.7 a 10.45 a
感病品种-大麻-感病品种(大庆) RS (Daqing) 37614 4140 5122.7 a 10.57 a
玉米-谷子-感病品种(齐齐哈尔) RQ (Qiqihar) 54387 4229 4972.7 a 10.20 b

表2

不同连作下大豆根际土壤细菌序列统计及多样性指数"

样品
Sample		 有效序列数量
Effective
sequence-number		 OTUs数量
OTUs number (97%)		 Chao指数
Chao index		 Shannon指数
Shannon index
连作4年(大庆) C4 (Daqing) 67271 4928 5659.6 a 10.47 a
连作10年(大庆) C10 (Daqing) 69356 4173 4583.8 b 10.23 b
连作20年(齐齐哈尔) C20 (Qiqihar ) 52387 3706 4147.0 b 10.09 c

图3

各样品在门水平的群落相对丰度"

图4

各样品在纲水平的群落相对丰度"

图5

样品聚类分析热图"

图6

6个土壤样品群落主成分分析"

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