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作物学报 ›› 2019, Vol. 45 ›› Issue (3): 411-418.doi: 10.3724/SP.J.1006.2019.84090

• 耕作栽培·生理生化 • 上一篇    下一篇

通过外源MeJA抑制H2O2积累提高烟草的耐冷性

马晓寒,张杰,张环纬,陈彪,温心怡,许自成()   

  1. 河南农业大学烟草学院, 河南郑州 450002
  • 收稿日期:2018-07-02 接受日期:2018-10-08 出版日期:2019-03-12 网络出版日期:2019-01-10
  • 通讯作者: 许自成
  • 作者简介:E-mail: maxiaohan1994@126.com
  • 基金资助:
    本研究由中国烟草总公司河南省公司科技攻关项目资助(201641170024100);本研究由中国烟草总公司河南省公司科技攻关项目资助(201641170024099)

Exogenous MeJA improves cold tolerance of tobacco by inhibiting H2O2 accumulation

Xiao-Han MA,Jie ZHANG,Huan-Wei ZHANG,Biao CHEN,Xin-Yi WEN,Zi-Cheng XU()   

  1. College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002, Henan, China
  • Received:2018-07-02 Accepted:2018-10-08 Published:2019-03-12 Published online:2019-01-10
  • Contact: Zi-Cheng XU
  • Supported by:
    This study was supported by the China National Tobacco Corporation’s Henan Provincial Science and Technology Research Project(201641170024100);This study was supported by the China National Tobacco Corporation’s Henan Provincial Science and Technology Research Project(201641170024099)

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摘要：

茉莉酸甲酯(MeJA)是可参与多种生理生化过程的激发子, 为探究外源MeJA对低温环境下烟草幼苗的影响, 以烟草品种“豫烟10号”为材料, 在其六叶一心时用4个不同浓度(1、10、100和1000 μmol L -1MeJA)进行喷施处理3 d后, 再进行低温处理, 同时以正常温度和低温处理作为阳性和阴性对照。分析各处理的长势指标、相对电解质渗透率、光合色素含量、抗氧化酶活性以及激素含量。结果表明: 在10 μmol L -1茉莉酸甲酯处理下能降低低温对烟草幼苗的损伤。然后在材料和时期不变的情况下, 进行DPI、10 μmol L -1 MeJA以及DPI+MeJA处理以低温处理为对照, 测定了H2O2、O 2-、CAT、MDA以及ASA-GSH循环的含量。证明在外源茉莉酸甲酯协同低温的处理下, 烟草植株中的H2O2主要作为毒害分子而非第二信使存在。

关键词: 低温胁迫, 烟草幼苗, 茉莉酸甲酯, H2O2传导

Abstract:

Methyl jasmonate (MeJA) is an elicitor evolving in a variety of physiological and biochemical processes. To explore the effects of exogenous MeJA on tobacco seedlings under low temperature conditions, we used the tobacco variety “Yuyan 10” and sprayed four concentrations (1, 10, 100, 1000 μmol L -1) of MeJA for 3 days on plants. Under low temperature with the treatment under normal temperature as positive and that under low temperature on negative control. The growth indicators, relative electrolyte permeability, photosynthetic pigment content, antioxidant enzyme activity and hormone content of each treatment were measured, showing that the treatment with 10 μmol L -1 methyl jasmonate could reduce the damage of tobacco seedlings caused by low temperature. The effect of H2O2 was then verified by applications of DPI, 10 μmol L -1 MeJA, and DPI+MeJA with the same material and growth period, while low temperature treatment was used as a control. The contents of H2O2, O 2-, CAT, MDA, and ASA-GSH cycle were determined. In conclusion, H2O2 in tobacco plants mainly exists as a poison molecule instead of a second messenger under the treatment of exogenous methyl jasmonate combined with low temperature.

Key words: low temperature stress, tobacco seedlings, MetJA, H2O2 conduction

表1

MeJA对低温胁迫下烟草幼苗地上部、根部的鲜重和干重的影响"

处理
Treatment		 鲜重Fresh weight 干重Dry weight
地上部 Aboveground 根 Root 地上部 Aboveground 根 Root
CK 25.52±1.45 a 3.56±0.27 a 3.74±0.52 a 0.30±0.04 a
C0 11.53±1.35 e 1.24±0.08 e 0.59±0.11 d 0.11±0.02 c
C1 12.85±0.52 de 1.13±0.12 de 0.85±0.09 d 0.12±0.05 c
C10 22.58±1.47 b 2.54±0.12 b 2.45±0.08 b 0.23±0.03 b
C100 19.28±0.78 c 2.08±0.04 c 1.84±0.10 c 0.22±0.02 b
C1000 14.03±0.68 d 1.45±0.19 d 0.92±0.09 d 0.11±0.01 c

图1

低温处理5 d内各处理的最大叶面积 缩写同表1。图柱上不同小写字母表示在0.05水平差异显著。"

图2

低温处理5 d后各处理的相对电导率和光合色素含量 缩写同表1。图柱上不同小写字母表示在0.05水平差异显著。"

图3

低温处理5 d后各处理的激素含量 缩写同表1。图柱上不同小写字母表示在0.05水平差异显著。"

图4

低温处理5 d后各处理的抗氧化酶系统活性 缩写同表1。图柱上不同小写字母表示在0.05水平差异显著。"

图5

低温下各处理的活性氧、CAT及MDA的含量 图柱上不同小写字母表示在0.05水平差异显著。"

图6

低温下各处理的GR、GSH、APX、ASA含量 图柱上不同小写字母表示在0.05水平差异显著。"

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