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作物学报 ›› 2020, Vol. 46 ›› Issue (02): 157-165.doi: 10.3724/SP.J.1006.2020.91048

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

小麦品种扬麦16赤霉病抗扩展QTL定位及分析

胡文静1,张勇1,陆成彬1,王凤菊2,刘金栋2,蒋正宁1,王金平2,朱展望2,徐小婷2,郝元峰2,何中虎2,3,高德荣1,*()   

  1. 1 江苏里下河地区农业科学研究所 / 农业农村部长江中下游小麦生物学与遗传育种重点实验室, 江苏扬州 225007
    2 国家小麦改良中心 / 中国农业科学院作物科学研究所, 北京 100081
    3 国际玉米小麦改良中心(CIMMYT)中国办事处, 北京 100081
  • 收稿日期:2019-07-22 接受日期:2019-09-26 出版日期:2020-02-12 网络出版日期:2019-10-09
  • 通讯作者: 高德荣
  • 作者简介:E-mail: huren2008@126.com
  • 基金资助:
    本研究由国家自然科学基金项目(31901544);国家现代农业产业技术体系建设专项(CARS-03-03B);国家现代农业产业技术体系建设专项(CARS-3-2-11);国家重点研发计划项目(2017YFD0100801);国家重点研发计划项目(2017YFD0101802);江苏省自然科学基金项目资助(BK20171279)

Mapping and genetic analysis of QTLs for Fusarium head blight resistance to disease spread in Yangmai 16

HU Wen-Jing1,ZHANG Yong1,LU Cheng-Bin1,WANG Feng-Ju2,LIU Jin-Dong2,JIANG Zheng-Ning1,WANG Jin-Ping2,ZHU Zhan-Wang2,XU Xiao-Ting2,HAO Yuan-Feng2,HE Zhong-Hu2,3,GAO De-Rong1,*()   

  1. 1 Lixiahe Institute of Agriculture Sciences / Key Laboratory of Wheat Biology and Genetic Improvement for Low & Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs, Yangzhou 225007, Jiangsu, China
    2 National Wheat Improvement Center / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
    3 CIMMYT-China Office, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-07-22 Accepted:2019-09-26 Published:2020-02-12 Published online:2019-10-09
  • Contact: De-Rong GAO
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31901544);China Agriculture Research System(CARS-03-03B);China Agriculture Research System(CARS-3-2-11);the National Key Research and Development Program of China(2017YFD0100801);the National Key Research and Development Program of China(2017YFD0101802);the Natural Science Foundation of Jiangsu Province(BK20171279)

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摘要：

扬麦系列品种赤霉病抗性在世界范围内得到重视, 但其抗性遗传机制尚不清楚。扬麦16是近年来大面积推广的抗赤霉病品种, 本研究以扬麦16与中麦895杂交构建的174个双单倍体(double haploid lines, DH)系为材料, 于2017—2019年连续3年对该群体采用单花滴注进行赤霉病抗扩展鉴定。利用660K SNP芯片构建高密度遗传图谱, 共检测到6个抗性QTL, 分别位于2DL、3BL、4BS、4DS、5BL和6AS染色体上。除4BS位点外, 其他5个抗性等位基因均来源于扬麦16。QFhb.yaas-4DS和QFhb.yaas-6AS均在多年被检测到, 可解释8.8%~15.0%的表型变异; QFhb.yaas-2DL、QFhb.yaas-3BL仅在1年被检测到, 分别解释10.5%和14.7%的表型变异; QFhb.yaas-5BL和来源于中麦895的QFhb.yaas-4BS仅在1年被检测到且效应仅为6.4%和8.3%。QTL效应分析结果表明, 相较于单个位点, 多个抗性QTL的聚合可显著降低赤霉病严重度。扬麦16抗赤霉病QTL将为揭示扬麦品种抗性遗传机制及开发相应分子标记奠定基础。

关键词: 小麦, 赤霉病, QTL, 标记辅助育种

Abstract:

Fusarium head blight (FHB) resistance of Yangmai wheat cultivars has been paid much attention, but the underlying genetic mechanism is unclear. In recent years, Yangmai 16 is a predominant wheat cultivar durably resistant to FHB in production. A population of 174 double haploid lines (DH) produced by crossing Yangmai 16 (YM16) with the susceptible cultivar Zhongmai 895 (ZM895) was evaluated for FHB response using point inoculation from 2017 to 2019. The DH population was genotyped with wheat 660K SNP array and a high-density genetic map was constructed. Six resistance QTLs were detected, and among them, five were from the resistant parent Yangmai 16 and one from Zhongmai 895. QFhb.yaas-4DS and QFhb.yaas-6AS were detected at least in two years, explaining 8.8% to 15.0% of the phenotypic variances, respectively. QFhb.yaas-2DL and QFhb.yaas-3BL were detected only in one year, accounting for 10.5% and 14.7% of the phenotypic variances. QFhb.yaas-5BL and QFhb.yaas-4BS were detected in one year, too, accounting for 6.4% and 8.3% of the phenotypic variances, respectively. Pyramiding of multiple resistant loci with large effects (>10%) is an effective approach to increase FHB resistance. The QTLs identified from Yangmai 16 in the present study will provide a starting point for genetic studies of other Yangmai cultivars, and the QTLs closely linked to markers will be useful for marker-assisted selection in wheat FHB improvement.

Key words: Triticum aestivum, Fusarium head blight, QTL, marker-assisted breeding

表1

扬麦16/中麦895 DH群体、亲本和对照的赤霉病严重度"

年份
Year		 亲本 Parents (%) 对照 CK (%) DH群体 DH population
扬麦16
Yangmai 16		 中麦895
Zhongmai 895		 苏麦3
Sumai 3		 周麦18
Zhoumai 18		 平均值
Mean (%)		 标准差
SD		 最大值
Max. (%)		 最小值
Min. (%)		 峰度
Kurt.		 偏度
Skew.		 遗传力
Hereditability
2017 14.2 A 58.7 B 5.2 A 60.2 B 29.1 18.9 83.7 3.4 0.2 0.9 0.67
2018 17.9 A 65.0 B 5.6 A 62.3 B 53.5 19.9 100.0 6.6 -0.7 -0.2 0.74
2019 23.3 A 69.7 B 6.1 A 70.1 B 49.5 18.1 89.8 10.3 -0.5 0.1 0.82

图1

扬麦16/中麦895 DH群体赤霉病严重度频次分布(2017-2019) 横坐标代表病小穗率, 纵坐标代表相关病小穗率范围内的家系数目。"

表2

扬麦16/中麦895 DH 群体抗赤霉病QTL定位"

QTL 年份
Year		 物理位置
Physical position (Mb)		 遗传位置
Genetic position (cM)		 标记区间
Marker interval		 LOD 贡献率
PVE (%)		 加性效应#
Add#
QFhb.yaas-2DL 2017 512.7-532.0 49.6 AX_111765614-AX_109738482 4.7 10.5 -6.1
QFhb.yaas-3BL 2019 637.1-647.2 138.6 AX_94528202-AX_109001202 8.9 14.7 -6.9
QFhb.yaas-4DS 2017 27.1-33.1 61.9 AX_109962849-AX_111071805 5.6 13.7 -7.2
2018 18.3-19.3 54.7 AX_111311200-AX_89421921 4.4 8.8 -5.9
2019 15.9-16.6 53.7 AX_94558069-AX_95150762 6.2 9.5 -5.6
QFhb.yaas-5BL 2018 587.1-588.8 138.7 AX_94646656-AX_109853998 3.2 6.4 -5.0
QFhb.yaas-6AS 2018 12.0-12.4 64.9 AX_109294414-AX_108848013 4.6 9.5 -6.2
2019 12.0-12.4 65.0 AX_109294414-AX_108848013 8.9 15.0 -7.1
QFhb.yaas-4BS 2018 82.7-91.3 14.7 AX_110472183-AX_94859572 4.1 8.3 5.8

图2

扬麦16/中麦895 DH群体抗赤霉病QTL定位情况 连锁群右边是标记名称, 左边是遗传位置(CM)。红色、蓝色和绿色分别代表2017、2018和2019年定位到的QTL, QTL右边对应LOD值。"

表3

扬麦16/中麦895 DH 群体中存在不同稳定赤霉病抗扩展QTL家系的赤霉病严重度情况"

QTL组成
QTL combination		 家系数
Number of lines		 最小值
Minimum		 最大值
Maximum		 平均
Mean		 标准差
Standard deviation		 方差
Variance
None 46 26.1 88.0 54.3 A 13.7 187.6
QFhb.yaas-4DS 62 18.6 71.3 43.2 B 13.1 172.0
QFhb.yaas-6AS 28 18.9 78.8 44.8 B 16.4 269.6
QFhb.yaas-4DS+QFhb.yaas-6AS 38 9.9 57.4 32.3 C 11.2 126.5

表4

扬麦16/中麦895 DH群体中存在不同赤霉病抗扩展QTL家系的赤霉病严重度情况"

QTL数
Number of QTL		 家系数
Number of lines		 平均PSS (100%)#
Average of PSS (100%)#		 不同PSS范围内的家系数目 Number of lines in different PSS range
0-25 25-50 50-75 75-100
0 7 67.1 A 0 0 6 1
1 46 53.8 B 1 15 28 2
2 65 41.8 C 8 38 19 0
3 46 36.9 CD 10 29 7 0
4 10 30.5 D 2 8 0 0

图3

DH家系所含QTL数目与其平均赤霉病严重度的关系 PSS (percentage of scabbed spikelets)代表赤霉病严重度。"

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