欢迎访问作物学报，今天是
作物学报 ›› 2023, Vol. 49 ›› Issue (12): 3261-3276.doi: 10.3724/SP.J.1006.2023.33010
所属专题： 玉米：遗传育种·种质资源·分子遗传学
钱甫1(
), 张占琴2, 陈树宾2, 丁永福2, 桑志勤2,*(), 李卫华1,*()
QIAN Fu1(), ZHANG Zhan-Qin2, CHEN Shu-Bin2, DING Yong-Fu2, SANG Zhi-Qin2,*(), LI Wei-Hua1,*()
摘要：
花期是玉米重要性状之一, 解析玉米花期的遗传基础, 挖掘玉米花期关键基因, 对于选育广适玉米品种具有重要意义。在580份玉米自交系构成的自然群体中, 3年种植测定散粉期、吐丝期和散粉吐丝间隔期等3个花期性状, 利用分布全基因组的31,826个SNPs (single nucleotide polymorphisms)标记进行全基因组关联分析(genome wide association study, GWAS), 结合自交系B73的14个不同发育阶段的转录组数据进行权重基因共表达网络分析(weighted gene co-expression network analysis, WGCNA), 挑选与玉米开花相关的组织特异性模块和关键基因。GWAS在多环境(2个环境以上)下共定位标记14个, 挖掘到潜在候选基因10个, WGCNA挖掘到花期潜在候选基因17个, 2种方法共同挖掘到候选基因3个。Zm00001d052180编码一个MADS-box转录因子19, Zm00001d016814编码NAC转录因子133, Zm00001d048082编码MADS-box转录因子8, 这些基因主要参与调节花序生长发育。研究结果为解析玉米花期遗传基础及分子机制提供参考。
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