作物学报 ›› 2023, Vol. 49 ›› Issue (5): 1222-1230.doi: 10.3724/SP.J.1006.2023.24140
陶顺玉(), 吴贝, 刘念, 罗怀勇, 黄莉, 周小静, 陈伟刚, 郭建斌, 喻博伦, 雷永, 廖伯寿, 姜慧芳()
TAO Shun-Yu(), WU Bei, LIU Nian, LUO Huai-Yong, HUANG Li, ZHOU Xiao-Jing, CHEN Wei-Gang, GUO Jian-Bin, YU Bo-Lun, LEI Yong, LIAO Bo-Shou, JIANG Hui-Fang()
摘要:
培育高含油量品种是花生重要的育种目标, 开发稳定高效的分子标记对于标记辅助选择花生高含油量品种具有重要价值。本研究针对2个含油量差异显著的亲本徐花13 (高含油量)和中花6号(低含油量), 利用PacBio三代测序技术检测基因组结构变异, 分别从徐花13和中花6号中检测到35,794个和74,703个结构变异。根据双亲结构变异信息, 针对前期定位的含油量区间开发InDel (插入/缺失)标记84个, 其中9个InDel标记的扩增片段证实在双亲间具有多态性。基于RIL (重组自交系)群体中的杂合残余获得的NIL (近等基因系)构建的一个包含1160个单株的F2群体, 使用9个多态性InDel标记鉴定其基因型, 构建了总长度为149.84 cM的局部遗传连锁图谱。结合F2群体的含油量表型数据, 将该含油量QTL定位在标记M23至M11之间, 位于A08染色体1.2 Mb区段内。本试验证实了InDel标记开展花生含油量QTL定位的可行性, 其定位的含油量位点及其紧密连锁的InDel标记能够为花生分子标记辅助育种提供理论和技术指导。
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