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作物学报 ›› 2023, Vol. 49 ›› Issue (8): 2051-2063.doi: 10.3724/SP.J.1006.2023.24208

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆叶型调控基因Ln及其同源基因单倍型分析

李刚1,3(), 周彦辰1, 熊亚俊1, 陈伊洁1, 郭庆元1, 高杰1, 宋健2, 王俊1,*(), 李英慧3,*(), 邱丽娟3,*()   

  1. 1 长江大学农学院, 湖北荆州 434025
    2 长江大学生命科学学院, 湖北荆州 434025
    3 中国农业科学院作物科学研究所, 北京 100081
  • 收稿日期:2022-09-10 接受日期:2023-02-21 出版日期:2023-08-12 网络出版日期:2023-03-01
  • 通讯作者: 王俊,李英慧,邱丽娟
  • 作者简介:李刚, E-mail: 1073855911@qq.com第一联系人：**同等贡献
  • 基金资助:
    云南省重大科技专项(202202AE090014)

Haplotype analysis of soybean leaf type regulator gene Ln and its homologous genes

LI Gang1,3(), ZHOU Yan-Chen1, XIONG Ya-Jun1, CHEN Yi-Jie1, GUO Qing-Yuan1, GAO Jie1, SONG Jian2, WANG Jun1,*(), LI Ying-Hui3,*(), QIU Li-Juan3,*()   

  1. 1 College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China
    2 College of Life Science, Yangtze University, Jingzhou 434025, Hubei, China
    3 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2022-09-10 Accepted:2023-02-21 Published:2023-08-12 Published online:2023-03-01
  • Contact: WANG Jun,LI Ying-Hui,QIU Li-Juan
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    Key Science and Technology Project of Yunnan Province(202202AE090014)

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摘要：

大豆叶型是理想株型的重要构成部分, 对大豆品种选育具有重要意义。本研究通过对来自中国不同地区的598份材料进行Ln及其同源基因(Glyma.10G273800)单倍型分析发现, Ln基因和同源基因均分别包含3种单倍型(Hap1~Hap3)。Ln基因3种单倍型之间除了叶长没有显著差异外, Hap2在叶宽、叶面积和叶长宽比中均与Hap1/Hap3差异显著。Ln同源基因的Hap3与Hap2在不同叶型表型中均有显著差异, Hap1和Hap2之间的叶长宽比没有差异, 叶宽在第4节叶位点没有显著差异, 第5节叶位点Hap1显著低于Hap2。叶长、叶面积、叶周长Hap1均显著低于Hap2。从地理分布来看, Ln基因的Hap2主要分布在高纬度和高海拔地区, 其同源基因的3种单倍型分布未发现明显地域倾向性。遗传分析表明仅Ln基因中Hap2的D9H存在人工选择的信号, Ln基因中的另外2个突变位点和同源基因中的2个突变位点则没有人工选择的信号。本研究为大豆叶型育种提供理论依据, 同时对提高大豆产量具有积极意义。

关键词: 大豆, 叶型, 单倍型, Ln基因

Abstract:

Soybean leaf type is an important component of ideal plant type, which is of great significance for the breeding of soybean varieties. In this study, we analyzed the haplotype of Ln and its homologous gene (Glyma.10G273800) in 598 materials from different regions of China. Ln gene and homologous gene contained three haplotypes (Hap1-Hap3), respectively. There was no significant difference in leaf length between the three haplotypes of Ln gene, and Hap2 was significantly different from Hap1/Hap3 in leaf width, leaf area, and leaf length width ratio. There were significant differences between Hap3 and Hap2 of Ln homologous gene in different leaf types. There was no difference in the ratio of leaf length to width between Hap1 and Hap2. There was no significant difference in leaf width phenotype at the 4th leaf site, and Hap1 at the 5th leaf site was significantly lower than Hap2. Leaf length, leaf area, and leaf circumference Hap1 were significantly lower than Hap2. In terms of geographical distribution, Hap2 of Ln gene was mainly distributed in high latitude and high altitude regions, and no obvious regional bias was detected in the distribution of the three haplotypes of its homologous genes. Genetic analysis showed that only D9H of Hap2 in Ln gene had artificial selection signal, while the other two mutation sites in Ln gene and two mutation sites in homologous gene had no artificial selection signal. This study provides the theoretical basis for soybean leaf type breeding and has positive significance for increasing soybean yield.

Key words: soybean, leaf type, haplotype, Ln gene

表1

598份大豆资源材料来源表、分类及地理信息"

来源
Origin		 数量
Number		 改良品种
Improved		 地方品种
Landrace		 经度
Longitude		 纬度
Latitude
安徽Anhui 21 6 15 117.17E 31.52N
北京Beijing 25 22 3 116.28E 39.54N
福建Fujian 6 2 4 118.04E 24.26N
广东Guangdong 11 1 10 113.18E 23.10N
甘肃Gansu 6 0 6 103.50E 36.03N
广西Guangxi 10 1 9 108.21E 22.47N
贵州Guizhou 33 0 33 106.72E 26.57N
海南Hainan 1 0 1 111.10E 20.03N
河北Hebei 48 7 41 114.26E 38.03N
河南Henan 24 11 13 113.40E 34.46N
黑龙Heilongjiang 76 59 17 126.64E 45.76N
湖北Hubei 28 5 23 115.32E 30.52N
湖南Hunan 15 3 12 112.59E 28.12N
吉林Jilin 45 17 28 125.18E 43.55N
江苏Jiangsu 27 10 17 118.75E 32.04N
江西Jiangxi 6 0 6 115.53E 28.42N
辽宁Liaoning 39 12 27 123.23E 41.48N
内蒙古Inner Mongolia 6 2 4 111.65E 40.82N
宁夏Ningxia 3 0 3 106.13E 38.28N
陕西Shaanxi 18 0 18 108.55E 34.15N
四川Sichuan 44 3 41 104.06E 30.67N
山东Shandong 29 9 20 120.18E 36.03N
上海Shanghai 2 0 2 121.43E 34.50N
山西Shanxi 56 11 45 112.33E 37.51N
台湾Taiwan 1 1 0 121.31E 25.02N
新疆Xinjiang 1 1 0 87.36E 43.46N
云南Yunnan 11 0 11 102.42E 25.03N
浙江Zhejiang 6 4 2 120.10E 30.15N

表2

598份材料不同叶型性状描述性统计"

性状
Trait		 节点
Node		 均值
Mean		 标准差
SD		 最小值
Min.		 最大值
Max.		 正态性检验显著性a
Normality test for significance a
叶面积Leaf area (cm2) 4 42.517 13.851 13.329 80.056 <0.001
5 45.803 13.486 16.780 81.826 <0.001
叶长Leaf length (cm) 4 10.206 1.421 6.294 14.009 >0.100
5 10.405 1.331 6.562 13.879 >0.100
叶长宽比Leaf length to width ratio 4 1.808 0.266 1.243 2.876 <0.001
5 1.685 0.279 1.130 2.634 <0.001
叶周长Leaf perimeter (cm) 4 27.817 4.161 16.584 40.053 >0.100
5 28.698 3.990 16.992 39.039 >0.100
叶宽Leaf width (cm) 4 5.950 1.346 3.077 9.693 <0.001
5 6.324 1.337 3.132 10.139 <0.001

表3

不同叶型性状相关性分析"

性状
Trait		 叶面积
Leaf area		 叶长
Leaf length		 叶长宽比
Leaf length to width ratio		 叶周长
Leaf perimeter		 叶宽
Leaf width
叶面积Leaf area 1
叶长Leaf length 0.777*** 1
叶长宽比Leaf length to width ratio -0.463*** 0.003 1
叶周长Leaf perimeter 0.801*** 0.772*** -0.231*** 1
叶宽Leaf width 0.956*** 0.612*** -0.654** 0.721*** 1

图1

Glyma.20G116200和Glyma.10G273800基因结构图"

表4

种质资源中Ln (Glyma.20G116200)及其同源基因(Glyma.10G273800)单倍型品种类型频率分布"

基因
Gene name		 单倍型
Haplotype		 类型
Class		 基因型
Genotype		 频率
Frequency		 改良品种
Improved		 地方品种
Landrace
Glyma.20G116200 Hap1 参考Reference GTA 429 92 337
Hap2 特定Specific CTA 59 55 4
Hap3 特定Specific GGT 92 34 58
Glyma.10G273800 Hap1 特定Specific TG 253 92 161
Hap2 参考Reference GG 206 82 124
Hap3 特定Specific GT 116 11 105

图2

Ln基因单倍型分析 A: 单倍型演化图; B: 突变类型及位点; C~F: 分别为第4节中间叶(C)、侧叶(D)、第5节中间叶(E)和侧叶(F)长宽比。显著性检验采用K-W检验, P < 0.01和P < 0.001分别为显著和极显著; G: Ln基因单倍型地理分布图。"

附图1

Ln基因不同单倍型叶型表型差异比较 A~D: 分别为第4节中间叶(A)、侧叶(B)、第5节中间叶(C)、侧叶(D)叶长比较; E~H: 分别为第4节中间叶(E)、侧叶(F)、第5节中间叶(G)、侧叶(H)叶宽比较; I~L: 分别为第4节中间叶(I)、侧叶(J)、第5节中间叶(K)、侧叶(L)叶面积比较; M~P: 分别为第4节中间叶(M)、侧叶(N)、第5节中间叶(O)、侧叶(P)叶周长比较。"

图3

Glyma.10G273800基因单倍型分析 A: 单倍型演化图; B: 突变类型及位点; C~F: 分别为第4节中间叶(C)、侧叶(D)、第5节中间叶(E)和侧叶(F)长宽比。显著性检验采用K-W检验, P < 0.01和P < 0.001分别为显著和极显著; G: Glyma.10G273800单倍型地理分布图。"

附图2

Glyma.10G273800不同单倍型叶型表型差异比较 A~D: 分别为第4节中间叶(A)、侧叶(B)、第5节中间叶(C)、侧叶(D)叶长比较; E~H: 分别为第4节中间叶(E)、侧叶(F)、第5节中间叶(G)、侧叶(H)叶宽比较; I~L: 分别为第4节中间叶(I)、侧叶(J)、第5节中间叶(K)、侧叶(L)叶面积比较; M~P: 分别为第4节中间叶(M)、侧叶(N)、第5节中间叶(O)、侧叶(P)叶周长比较。"

图4

Ln基因及同源基因遗传分化分析 A: Ln基因群体间遗传分化指数; B: Ln基因中性检验; C: Ln基因种群核苷酸多样性分析; D: Glyma.10G273800群体间遗传分化指数; E: Glyma.10g273800中性检验; F: Glyma.10g273800种群核苷酸多样性分析。Fst: 群体间遗传分化指数; Tajima’s D: 中性检验; π: 种群核苷酸多样性分析。"

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