卷叶木薯及其突变体叶片的比较转录组分析

doi:10.3724/SP.J.1006.2024.34168

作物学报 ›› 2024, Vol. 50 ›› Issue (8): 2143-2156.doi: 10.3724/SP.J.1006.2024.34168

• 研究简报 • 上一篇

卷叶木薯及其突变体叶片的比较转录组分析

肖明昆¹(), 严炜¹^,²^,^*(), 宋记明¹^,²(), 张林辉¹^,², 刘倩¹, 段春芳¹^,², 李月仙¹^,², 姜太玲¹^,², 沈绍斌¹^,², 周迎春¹, 沈正松¹^,², 熊贤坤¹, 罗鑫¹, 白丽娜¹, 刘光华³^,^*()

¹云南省农业科学院热带亚热带经济作物研究所, 云南保山 678000
²热带作物生物育种全国重点实验室, 云南昆明 650205
³云南省农业科学院国际农业研究所, 云南昆明 650205

收稿日期:2023-10-15 接受日期:2024-04-01 出版日期:2024-08-12 网络出版日期:2024-04-24
通讯作者: * 严炜, E-mail: rjsyw@yaas.org.cn;刘光华, E-mail: rjslgh@vip.126.com
作者简介:肖明昆, E-mail: nkyxmk@163.com;
宋记明, E-mail: 821427168@qq.com
^** 同等贡献
基金资助:
兴滇英才支持计划项目——云南优良饲草品种选育及产业化关键技术集成示范和财政部和农业农村部国家现代农业产业技术体系建设专项(木薯-保山综合试验站);兴滇英才支持计划项目——云南优良饲草品种选育及产业化关键技术集成示范和财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-11-YNLGH)

Comparative transcriptome profiling of leaf in curled-leaf cassava and its mutant

XIAO Ming-Kun¹(), YAN Wei¹^,²^,^*(), SONG Ji-Ming¹^,²(), ZHANG Lin-Hui¹^,², LIU Qian¹, DUAN Chun-Fang¹^,², LI Yue-Xian¹^,², JIANG Tai-Ling¹^,², SHEN Shao-Bin¹^,², ZHOU Ying-Chun¹, SHEN Zheng-Song¹^,², XIONG Xian-Kun¹, LUO Xin¹, BAI Li-Na¹, LIU Guang-Hua³^,^*()

¹Tropical and Subtropical Cash Crops Research Institute, Yunnan Academy of Agricultural Sciences, Baoshan 678000, Yunnan, China
²National Key Laboratory of Tropical Crop Breeding, Kunming 650205, Yunnan, China
³International Agricultural Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, Yunnan, China

Received:2023-10-15 Accepted:2024-04-01 Published:2024-08-12 Published online:2024-04-24
Contact: * E-mail: rjsyw@yaas.org.cn;E-mail: rjslgh@vip.126.com
About author:^** Contributed equally to this work
Supported by:
Xingdian Talent Support Plan Project: Integrated Demonstration of Key Technologies for the Breeding and Industrialization of Excellent Forage Varieties in Yunnan, and the China Agriculture Research System of MOF and MARA (Cassava-Comprehensive Experimental Station Project);Xingdian Talent Support Plan Project: Integrated Demonstration of Key Technologies for the Breeding and Industrialization of Excellent Forage Varieties in Yunnan, and the China Agriculture Research System of MOF and MARA(CARS-11-YNLGH)

摘要/Abstract

摘要：

为解析卷叶木薯叶片异常发育的分子调控机制及代谢通路, 以正常卷叶木薯叶片(JY)、突变株展叶叶片(ZY)和突变株卷叶叶片(BJ)为试验材料, 基于转录组测序(RNA-seq)数据进行生物信息学分析。DESeq差异分析结果显示, ZY vs BJ、JY vs ZY、JY vs BJ分别有327 (255个上调, 72个下调)、1085 (337个上调, 748个下调)、689 (381个上调, 308个下调)个DEGs, 有19个DEGs是3个比较组共同表达的。GO功能分析显示, DEGs在刺激反应、膜的组成部分、跨膜转运蛋白活性等途径差异显著。KEGG富集分析显示, DEGs在苯丙素的生物合成、植物激素信号转导、内质网中的蛋白加工等通路较为活跃。进一步对变异展叶与同株的卷叶和其他植株正常卷叶的DEGs分析发现集中富集到苯丙素的生物合成途径、淀粉和蔗糖代谢、植物激素信号转导, 这可能是展叶突变体形成的关键因子。展叶与卷叶差异基因的主要KEGG代谢通路有9条, 重要差异基因有9个。对不同类型叶片显微分析发现突变后的展叶木薯上表皮细胞层数增加、海绵组织结构变得疏松、维管束细胞减少。研究结果为进一步理解木薯叶片异常发育的分子机制提供了理论指导, 为木薯遗传改良、种质创新挖掘提供基因资源和改良策略。

关键词: 木薯, 转录组分析, 突变, 差异基因表达

Abstract:

The objective of this study is to elucidate the molecular regulatory mechanisms and metabolic pathways underlying the abnormal development in the leaf of curled-leaf cassava. Normal curled cassava leaf (JY), mutant expanded leaf (ZY), and mutant curled leaf (BJ) were used as the experimental materials, and bioinformatics analysis was conducted based on transcriptome sequencing (RNA-seq) data. DESeq differential analysis showed that there were 327 (255 up-regulated, 72 down-regulated) DEGs for ZY vs BJ, 1085 (337 up-regulated, 748 down-regulated) DEGs for JY vs ZY, and 689 (381 up-regulated, 308 down-regulated) DEGs for JY vs BJ, and 19 DEGs were co-expressed by three comparative groups. GO functional analysis showed that DEGs had significant differences in the pathways of stimulus response, components of membranes, and transmembrane transporter protein activity. KEGG enrichment analysis indicated that the DEGs were more active in pathways such as phenylpropanoid biosynthesis, phytohormone signaling, and protein processing in the endoplasmic reticulum. Further analysis of the DEGs in the mutated leaf expansion, curled leaves of the same plant, and normal curled leaves of other plants, demonstrated that the DEGs were concentrated in the phenylpropanoid biosynthesis pathways, starch and sucrose metabolism, and plant hormone signal transduction, which may be the key factors causing leaf expansion mutants. There were 9 main KEGG metabolic pathways and 9 important differentially expressed genes involved in leaf expansion and leaf curling. Microscopic analysis of different types of leaf revealed an increase in the number of epidermal cell layers, a loosening of sponge tissue structure, and a decrease in vascular bundle cells in mutated expanded cassava. The results of this study provide theoretical guidance for further understanding the molecular mechanisms underlying the abnormal development of cassava leaf, as well as genetic resources and improvement strategies for cassava genetic improvement and germplasm innovation.

Key words: cassava, transcriptome analysis, mutation, differential gene expression

肖明昆, 严炜, 宋记明, 张林辉, 刘倩, 段春芳, 李月仙, 姜太玲, 沈绍斌, 周迎春, 沈正松, 熊贤坤, 罗鑫, 白丽娜, 刘光华. 卷叶木薯及其突变体叶片的比较转录组分析[J]. 作物学报, 2024, 50(8): 2143-2156.

XIAO Ming-Kun, YAN Wei, SONG Ji-Ming, ZHANG Lin-Hui, LIU Qian, DUAN Chun-Fang, LI Yue-Xian, JIANG Tai-Ling, SHEN Shao-Bin, ZHOU Ying-Chun, SHEN Zheng-Song, XIONG Xian-Kun, LUO Xin, BAI Li-Na, LIU Guang-Hua. Comparative transcriptome profiling of leaf in curled-leaf cassava and its mutant[J]. Acta Agronomica Sinica, 2024, 50(8): 2143-2156.

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参考文献 64

[1]	Ano C U, Ochwo-Ssemakula M, Ibanda A, Ozimati A, Gibson P, Onyeka J, Njoku D, Egesi C, Kawuki R S. Cassava brown streak disease response and association with agronomic traits in elite Nigerian cassava cultivars. Front Plant Sci, 2021, 12: 720532.
[2]	Ceballos H, Hershey C, Iglesias C, Zhang X F. Fifty years of a public cassava breeding program: evolution of breeding objectives, methods, and decision-making processes. Theor Appl Genet, 2021, 134: 2335-2353. doi: 10.1007/s00122-021-03852-9 pmid: 34086085
[3]	刘翠娟, 郑刚辉, 梁海波. 不同种植方式对华南205木薯产量的影响. 现代农业科技, 2016, (14): 15-16.
	Liu C J, Zheng G H, Liang H B. Effects of different planting methods on the yield of cassava South China 205. Modern Agric Sci Technol, 2016, (14): 15-16 (in Chinese).
[4]	刘月娥, 徐田军, 蔡万涛, 吕天放, 张勇, 薛洪贺, 王荣焕, 赵久然. 我国玉米超高产研究现状与展望. 生物技术通报, 2023, 39(8): 52-61. doi: 10.13560/j.cnki.biotech.bull.1985.2023-0555
	Liu Y E, Xu T J, Cai W T, Lyu T F, Zhang Y, Xue H H, Wang R H, Zhao J R. Current status and prospects of maize super high yield research in China. Biotechnol Bull, 2023, 39(8): 52-61 (in Chinese with English abstract).
[5]	Jathar V, Saini K, Chauhan A, Rani R, Ichihashi Y, Ranjan A. Spatial control of cell division by GA-OSGRF7/8 module in a leaf explains the leaf length variation between cultivated and wild rice. New Phytol, 2022, 234: 867-883.
[6]	Zhang T, Li C, Li D, Liu Y, Yang X. Roles of YABBY transcription factors in the modulation of morphogenesis, development, and phytohormone and stress responses in plants. J Plant Res, 2020, 133: 751-763. doi: 10.1007/s10265-020-01227-7 pmid: 33033876
[7]	Wang H F, Kong F J, Zhou C. From genes to networks: the genetic control of leaf development. J Integr Plant Biol, 2021, 63: 1181-1196. doi: 10.1111/jipb.13084
[8]	孙虎声. 卷叶型和展叶型水稻的生理、生长性状的对比研究. 南京信息工程大学硕士学位论文, 江苏南京, 2006.
	Sun H S. Comparison of Rice Physiology and Growth between Curled-Leaf and Uncurled-Leaf Varieties. MS Thesis of Nanjing University of Information Science & Technology, Nanjing, Jiangsu, China, 2006 (in Chinese with English abstract).
[9]	黄桂荣. 冬小麦品种间水分利用效率差异及其关键影响因素分析. 中国农业科学院博士学位论文, 北京, 2021.
	Huang G R. Analysis of Differences in Water Use Efficiency among Winter Wheat Cultivars and Key Influencing Factors. PhD Dissertation of Chinese Academy of Agricultural Sciences, Beijing, China, 2021 (in Chinese with English abstract).
[10]	Hayakawa Y, Tachikawa M, Mochizuki A. Flat leaf formation realized by cell-division control and mutual recessive gene regulation. J Theor Biol, 2016, 404: 206-214. doi: S0022-5193(16)30139-4 pmid: 27287339
[11]	贾越. 生菜叶卷曲控制基因的遗传克隆与分子机制. 华中农业大学博士学位论文, 湖北武汉, 2022.
	Jia Y. Genetic and Molecular Mechanisms Underlying Lettuce Wavy Leaves. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2022 (in Chinese with English abstract).
[12]	Nath U, Crawford B C, Carpenter R, Coen E. Genetic control of surface curvature. Science, 2003, 299: 1404-1407. doi: 10.1126/science.1079354 pmid: 12610308
[13]	Palatnik J F, Allen E, Wu X L, Schommer C, Schwab R, Carrington J C, Weigel D. Control of leaf morphogenesis by microRNAs. Nature, 2003, 425: 257-263.
[14]	Alvarez J P, Goldshmidt A, Efroni I, Bowman J L, Eshed Y. The NGATHA distal organ development genes are essential for style specification in Arabidopsis. Plant Cell, 2009, 21: 1373-1393. doi: 10.1105/tpc.109.065482 pmid: 19435933
[15]	Chen N, Yu B, Dong R, Lei J J, Chen C M, Cao B H. RNA-Seq-derived identification of differential transcription in the eggplant (Solanum melongena) following inoculation with bacterial wilt. Gene, 2018, 644: 137-147. doi: S0378-1119(17)30936-8 pmid: 29104166
[16]	杨亚杰, 李昱樱, 申状状, 陈天, 荣二花, 吴玉香. 草棉不同倍性材料叶片转录组差异表达分析. 作物学报, 2022, 48: 2733-2748. doi: 10.3724/SP.J.1006.2022.14168
	Yang Y J, Li Y Y, Shen Z Z, Chen T, Rong E H, Wu Y X. Differential expressed analysis by transcriptome sequencing in leaves of different ploidy Gossypium herbaceum. Acta Agron Sin, 2022, 48: 2733-2748 (in Chinese with English abstract). doi: 10.3724/SP.J.1006.2022.14168
[17]	刘敏, 黄炜忠, 何孟璐, 梅瑜, 王继华. 大青转录组测序及生物信息学分析. 广州中医药大学学报, 2022, 39: 177-183.
	Liu M, Huang W Z, He M L, Mei Y, Wang J H. Transcriptome sequencing and bioinformatic analysis of Clerodendron cyrtophyllum Turez. J Guangzhou Univ Tradit Chin Med, 2022, 39(1): 177-183 (in Chinese with English abstract).
[18]	韦丽君, 俞奔驰, 宋恩亮, 郑华, 卢赛清, 付海天. 基于转录组测序的木薯性别决定相关基因挖掘. 南方农业学报, 2020, 51: 1785-1796.
	Wei L J, Yu B C, Song E L, Zheng H, Lu S Q, Fu H T. Gene mining for sex determination in cassava (Manihot esculenta Crantz) based on transcriptome sequencing. J South Agric, 2020, 51: 1785-1796 (in Chinese with English abstract).
[19]	张雨晨, 王国镇, 洪启浩, 刘紫佳, 郑雨晨, 唐铎, 周志祥. 基于转录组测序探究PM 2.5引起THP-1细胞发生炎性反应的关键因子. 环境科学学报, 2023, 43: 454-463.
	Zhang Y C, Wang G Z, Hong Q H, Liu Z J, Zheng Y C, Tang D, Zhou Z X. Transcriptome sequencing to explore key factors in the inflammatory response of PM2.5 induced THP-1 cells. Acta Sci Circumstant, 2023, 43: 454-463 (in Chinese with English abstract).
[20]	刘鹏, 毕江涛, 李文兵, 惠治兵, 肖国举, 孙权, 王静. 生物有机肥对盐碱地水稻叶片的影响转录组分析. 生态学报, 2022, 42: 2342-2356.
	Liu P, Bi J T, Li W B, Hui Z B, Xiao G J, Sun Q, Wang J. Transcriptome analysis of the effect of bio-organic fertilizer on rice leaves. Acta Ecol Sin, 2022, 42: 2342-2356 (in Chinese with English abstract).
[21]	范小锋, 古佳玉, 赵明辉, 赵林姝, 郭会君, 熊宏春, 谢永盾, 赵世荣, 丁玉萍, 乔文臣, 徐延浩, 刘录祥. 小麦直立叶突变体MtHS29的转录组分析. 植物遗传资源学报, 2022, 23: 871-880. doi: 10.13430/j.cnki.jpgr.20211115001
	Fan X F, Gu J Y, Zhao M H, Zhao L S, Guo H J, Xiong H C, Xie Y D, Zhao S R, Ding Y P, Qiao W C, Xu Y H, Liu L X. Transcriptome analysis of wheat erect leaf mutant MtHS29. J Plant Genet Resour, 2022, 23: 871-880 (in Chinese with English abstract).
[22]	苏湘杰, 岳晓楠, 卢银, 解紫薇, 王彦华, 赵建军, 刘梦洋. 大白菜叶色深绿突变dg的转录组差异表达调控分析. 河北农业大学学报, 2023, 46(4): 22-32.
	Su X J, Yue X N, Lu Y, Xie Z W, Wang Y H, Zhao J J, Liu M Y. Differential expression regulation analysis of dark-green leaf mutant dg in Chinese cabbage. J Hebei Agric Univ, 2023, 46(4): 22-32 (in Chinese with English abstract).
[23]	吴连成, 李沛, 田磊, 王顺喜, 李明娜, 王宇宇, 王赛, 陈彦惠. 阻断授粉诱导玉米叶片提前衰老的转录组分析. 作物学报, 2018, 44: 1661-1672. doi: 10.3724/SP.J.1006.2018.01661
	Wu L C, Li P, Tian L, Wang S X, Li M N, Wang Y Y, Wang S, Chen Y H. Transcriptome analysis of premature senescence induced by pollination-prevention in maize. Acta Agron Sin, 2018, 44: 1661-1672 (in Chinese with English abstract).
[24]	Luo X Q, An F F, Xue J J, Zhu W L, Wei Z W, Ou W J, Li K M, Chen S B, Cai J. Integrative analysis of metabolome and transcriptome reveals the mechanism of color formation in cassava (Manihot esculenta Crantz) leaves. Front Plant Sci, 2023, 14: 1181257.
[25]	Ding Z H, Zhang Y, Xiao Y, Liu F F, Wang M H, Zhu X G, Liu P, Sun Q, Wang W Q, Peng M, Brutnell T, Li P H. Transcriptome response of cassava leaves under natural shade. Sci Rep, 2016, 6: 31673. doi: 10.1038/srep31673 pmid: 27539510
[26]	韦丽君, 俞奔驰, 吕平, 檀小辉, 雷开文, 马崇熙, 卢赛清, 唐玉娟. 木薯叶芽和花芽的转录组差异分析. 分子植物育种, 2020, 18: 2108-2117.
	Wei L J, Yu B C, Lyu P, Tan X H, Lei K W, Ma C X, Lu S Q, Tang Y J. Transcriptome difference analysis of cassava (Manihot esculenta) leaf bud and flower bud. Mol Plant Breed, 2020, 18: 2108-2117 (in Chinese with English abstract).
[27]	张书敏, 杨尚君, 刘红云, 刘征. 微波快速石蜡切片法观察谷子幼嫩叶片组织技术的优化. 基因组学与应用生物学, 2015, 34: 669-673.
	Zhang S M, Yang S J, Liu H Y, Liu Z. Optimization of microwave paraffin techniques for clear observation of cell structures of millet (Setaria italica) young leaves. Genomics Appl Biol, 2015, 34: 669-673 (in Chinese with English abstract).
[28]	张贤, 王建红, 喻曼, 曹凯, 庄俐, 徐昌旭, 曹卫东. 基于RNA-seq的能源植物芒转录组分析. 生物工程学报, 2015, 31: 1437-1448.
	Zhang X, Wang J H, Yu M, Cao K, Zhuang L, Xu C X, Cao W D. Transcriptome analysis of bioenergy plant Miscanthus sinensis Anderss. by RNA-Seq. Chin J Biotechnol, 2015, 31: 1437-1448 (in Chinese with English abstract).
[29]	翟莹. 不同光质和基质对韭菜生长及营养品质影响规律研究. 东北农业大学硕士学位论文, 黑龙江哈尔滨, 2018.
	Zhai Y. Effect of Different Light Quality and Substrate on the Growth and Nutritional Quality of Chinese Chive. MS Thesis of Northeast Agricultural University, Harbin, Heilongjiang, China, 2018 (in Chinese with English abstract).
[30]	韦云飞, 白璐嘉, 宋晓叶, 肖晓荣, 马启林. 基于水稻幼穗盐胁迫响应转录组的MYB基因分析及耐盐基因挖掘. 分子植物育种, 2023, 21: 360-369.
	Wei Y F, Bai L J, Song X Y, Xiao X R, Ma Q L. Transcriptome analysis of MYB based on salt stress response in young rice panicles and mining of salt tolerance genes. Mol Plant Breed, 2023, 21: 360-369 (in Chinese with English abstract).
[31]	Jia Y, Yu P, Shao W, An G H, Chen J J, Yu C C, Kuang H H, Up-regulation of LsKN1 promotes cytokinin and suppresses gibberellin biosynthesis to generate wavy leaves in lettuce. J Exp Bot, 2022, 73: 6615-6629.
[32]	李祥燕. 白掌叶片生长规律研究及转录组测序分析. 华南农业大学硕士学位论文, 广东广州, 2020.
	Li X Y. Research on Growth Rhythm and Transcriptome Analysis of Leaves of Spathiphyllum. MS Thesis of South China Agricultural University, Guangzhou, Guangdong, China, 2020 (in Chinese with English abstract).
[33]	Hupp S, Rosenkranz M, Bonfig K, Pandey C, Roitsch T. Noninvasive phenotyping of plant-pathogen interaction: consecutive in situ imaging of fluorescing pseudomonas syringae, plant phenolic fluorescence, and chlorophyll fluorescence in Arabidopsis leaves. Front Plant Sci, 2019, 10: 1239.
[34]	de Wit P J G M. How plants recognize pathogens and defend themselves. Cell Mol Life Sci, 2007, 64: 2726-2732. pmid: 17876517
[35]	Zipfel C. Pattern-recognition receptors in plant innate immunity. Curr Opin Immunol, 2008, 20: 10-16. doi: 10.1016/j.coi.2007.11.003 pmid: 18206360
[36]	Cal A J, Sanciangco M, Rebolledo M C, Luquet D, Torres R O, McNally K L, Henry A. Leaf morphology, rather than plant water status, underlies genetic variation of rice leaf rolling under drought. Plant Cell Environ, 2019, 42: 1532-1544.
[37]	范玉斌, 梁婉琪. 水稻叶极性发育分子机制研究进展. 上海交通大学学报(农业科学版), 2014, 32(1): 16-22.
	Fan Y B, Liang W Q. Research progress on the mechanism of leaf polarity establishment in rice. J Shanghai Jiaotong Univ (Agric Sci), 2014, 32(1): 16-22 (in Chinese with English abstract).
[38]	王灿, 王艳芳, 张应华, 许俊强. 泛基因组学在植物中的应用研究进展. 湖南生态科学学报, 2020, 7(2): 51-58.
	Wang C, Wang Y F, Zhang Y H, Xu J Q. Research progress on the applications of pan-genomics in plants. J Hunan Ecol Sci, 2020, 7(2): 51-58 (in Chinese with English abstract).
[39]	王雅利. 谷子类胡萝卜素含量合成的相关性分析及其SiERF-ANT基因的表达模式研究. 山西师范大学硕士学位论文, 山西太原, 2022.
	Wang Y L. Correlation Analysis of Carotenoid Content Synthesis in Millet and Its Expression Pattern of SiERF-ANT Gene. MS Thesis of Shanxi Normal University, Taiyuan, Shanxi, China, 2022 (in Chinese with English abstract).
[40]	秦智. 基于转录组测序和QTL定位的草莓香型葡萄香气相关基因挖掘. 沈阳农业大学硕士学位论文, 辽宁沈阳, 2021.
	Qin Z. Aroma-related Gene Mining in Labrusca-flavored Grapes Based on Transcriptome Sequencing and QTL Mapping. MS Thesis of Shenyang Agricultural University, Shenyang, Liaoning, China, 2021 (in Chinese with English abstract).
[41]	赵芳明, 魏霞, 马玲, 桑贤春, 王楠, 张长伟, 凌英华, 何光华. 水稻生育后期卷叶突变体lrl1的鉴定及基因定位和候选基因预测. 科学通报, 2015, 60: 3133-3143.
	Zhao F M, Wei X, Ma L, Sang X C, Wang N, Zhang C W, Ling Y H, He G H. Identification, gene mapping and candidate gene prediction of a late-stage rolled leaf mutant lrl1in rice (Oryza sativa L.). Chin Sci Bull, 2015, 60: 3133-3143 (in Chinese with English abstract).
[42]	Hammerschmidt R. PHYTOALEXINS: what have we learned after 60 years? Annu Rev Phytopathol, 1999, 37: 285-306. pmid: 11701825
[43]	Baslam M, Toshiaki M, Kuni S, Takuji O. Recent advances in carbon and nitrogen metabolism in C₃ plants. Int J Mol Sci, 2021, 22: 318.
[44]	李良良, 李燕, 丁贵杰. 高温和干旱对木本植物碳代谢影响的研究进展. 山地农业生物学报, 2022, 41(6): 30-36.
	Li L L, Li Y, Ding G J. Research progress on effects of high temperature and drought on carbon metabolism in woody plants. J Mount Agric Biol, 2022, 41(6): 30-36 (in Chinese with English abstract).
[45]	Chandel S S, Sharma K D. Down-regulation of carbohydrate metabolic pathway genes lowers sucrose and starch content in chickpea leaves under high temperature stress. Natl Acad Sci Lett, 2023, 46: 445-449.
[46]	Yin X, Yang D N, Liu Y M, Yang S H, Zhang R, Sun X L, Liu H X, Duan Y W, Yang Y Q, Yang Y P. Sophora moorcroftiana genome analysis suggests association between sucrose metabolism and drought adaptation. Plant Physiol, 2023, 19: 844-848.
[47]	Gómez-Vásquez R, Day R, Buschmann H, Randles S, Beeching J R, Cooper R M. Phenylpropanoids, phenylalanine ammonia lyase and peroxidases in elicitor-challenged cassava (Manihot esculenta) suspension cells and leaves. Ann Bot, 2024, 19: 87-97.
[48]	Kavil S, Otti G, Bouvaine S, Armitage A, Maruthi M N. PAL1 gene of the phenylpropanoid pathway increases resistance to the cassava brown streak virus in cassava. Virol J, 2021, 18: 184. doi: 10.1186/s12985-021-01649-2 pmid: 34503522
[49]	Fu L L, Ding Z H, Tie W W, Yan Y, Hu W, Zhang J M. Large- scale RNA-seq analysis reveals new insights into the key genes and regulatory networks of anthocyanin biosynthesis during development and stress in cassava. Ind Crops Prod, 2021, 169: 113627.
[50]	陈会鲜, 梁振华, 黄珍玲, 韦婉羚, 张秀芬, 杨海霞, 李锐, 何文, 李天元, 兰秀, 阮丽霞, 蔡兆琴, 农君鑫. 木薯花性别分化关键时期的转录组分析及雌花分化相关候选基因的筛选. 作物学报, 2023, 49: 2863-2875.
	Chen H X, Liang Z H, Huang Z L, Wei W L, Zhang X F, Yang H X, Li R, He W, Li T Y, Lan X, Ruan L X, Cai Z Q, Nong J X. Transcriptomic profile of key stages of sex differentiation in cassava flowers and discovery of candidate genes related to female flower differentiation. Acta Agron Sin, 2023, 49: 2863-2875 (in Chinese with English abstract).
[51]	刘嘉伟. 高加索三叶草根瘤菌多样性及结瘤固氮机制研究. 内蒙古农业大学硕士学位论文, 内蒙古呼和浩特, 2022.
	Liu J W. Rhizobium Diversity and Nodulation and Nitrogen Fixation Mechanism in Trifolium ambiguum. MS Thesis of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China, 2022 (in Chinese with English abstract).
[52]	Qi J Y, Wu B B, Feng S L, Lu S Q, Guan C M, Zhang X, Qiu D L, Hu Y C, Zhou Y H, Li C Y, Long M, Jiao Y L. Mechanical regulation of organ asymmetry in leaves. Nat Plants, 2017, 3: 724-733. doi: 10.1038/s41477-017-0008-6 pmid: 29150691
[53]	Guan C M, Wu B B, Yu T, Wang Q Q, Krogan N T, Liu X G, Jiao Y L. Spatial auxin signaling controls leaf flattening in Arabidopsis. Curr Biol, 2017, 27: 2940-2950.
[54]	Xiong Y Y, Wu B B, Du F, Guo X L, Tian C H, Hu J R, Lü S Q, Long M, Zhang L, Wang Y, Jiao Y L. A crosstalk between auxin and brassinosteroid regulates leaf shape by modulating growth anisotropy. Mol Plant, 2021, 14: 949-962. doi: 10.1016/j.molp.2021.03.011 pmid: 33722761
[55]	Yu X L, Guo X, Zhao P J, Li S X, Zou L P, Li W B, Xu Z Y, Peng M, Ruan M B. A homeodomain-leucine zipper I transcription factor, MeHDZ14, regulates internode elongation and leaf rolling in cassava (Manihot esculenta Crantz). Crop J, 2023, 11: 1419-1430.
[56]	赵亚林, 闫青地, 冯佳佳, 肖锋, 杨卓, 王凤茹, 董金泉. 石蜡切片方法的改良. 安徽农业科学, 2017, 45(32): 6-8.
	Zhao Y L, Yan Q D, Feng J J, Xiao F, Yang Z, Wang F R, Dong J Q. Method improvement of paraffin section. J Anhui Agric Sci, 2017, 45(32): 6-8 (in Chinese with English abstract).
[57]	张俊环, 张美玲, 杨丽, 姜凤超, 于文剑, 王玉柱, 孙浩元. 基于叶片显微结构综合评价杏不同品种(系)的抗旱性. 果树学报, 2023, 40: 2050-2060.
	Zhang J H, Zhang M L, Yang L, Jiang F C, Yu W J, Wang Y Z, Sun H Y. Comprehensive evaluation of drought resistance of different apricot cultivars (lines) based on leaf microstructure. J Fruit Sci, 2023, 40: 2050-2060 (in Chinese with English abstract).
[58]	王兆成, 王磊, 周梦钰, 何的明, 毕慧慧, 葛翔, 沈军城, 傅松玲. 3个薄壳山核桃品种叶片结构特征和枝条导水功能比较. 植物资源与环境学报, 2021, 30(3): 38-45.
	Wang Z C, Wang L, Zhou M Y, He D M, Bi H H, Ge X, Shen J C, Fu S L. Comparison on leaf structure characteristics and branch hydraulic function of three Carya illinoinensis cultivars. J Plant Resour Environ, 2021, 30(3): 38-45 (in Chinese with English abstract).
[59]	丁祥, 钟海霞, 王西平, 宋军阳, 吴久赟, 刘国宏, 张付春, 胡鑫, 潘明启, 伍新宇. 新疆葡萄砧木叶片解剖结构观察及抗旱性评价. 分子植物育种, 2023, 22: 7318-7324.
	Ding X, Zhong H X, Wang X P, Song J Y, Wu J Y, Liu G H, Zhang F C, Hu X, Pan M Q, Wu X Y. Observation on leaf anatomical structure and evaluation of drought resistance of grape rootstocks in Xinjiang. Mol Plant Breed, 2023, 22: 7318-7324 (in Chinese with English abstract).
[60]	黄州, 杜志喧, 王建平, 李剑镔, 鲍建中, 任伟芳, 傅军如. 水稻卷叶性状与分子调控机制研究进展. 分子植物育种, 2021, 19: 7604-7611.
	Huang Z, Du Z X, Wang J P, Li J B, Bao J Z, Ren W F, Fu J R. Research progress on rolled leaf traits and molecular regulation mechanism in rice (Oryza sativa). Mol Plant Breed, 2021, 19: 7604-7611 (in Chinese with English abstract).
[61]	秦茜, 朱俊杰, 关心怡, 于天卉, 曹坤芳. 七个甘蔗品种叶片解剖结构特征与光合能力和耐旱性的关联. 植物生理学报, 2017, 53: 705-712.
	Qin X, Zhu J J, Guan X Y, Yu T H, Cao K F. The correlations of leaf anatomical characteristics with photosynthetic capacity and drought tolerance in seven sugarcane cultivars. Plant Physiol J, 2017, 53: 705-712 (in Chinese with English abstract).
[62]	马胜, 齐恩芳, 文国宏, 李掌, 曲亚英, 郑永伟, 白永杰, 贾小霞. 基于叶片显微结构综合评价马铃薯不同品种的抗旱性. 中国马铃薯, 2021, 35: 500-506.
	Ma S, Qi E F, Wen G H, Li Z, Qu Y Y, Zheng Y W, Bai Y J, Jia X X. Leaf microstructure comprehensive evaluation of drought resistance of different potato varieties based on. Chin Potato J, 2021, 35: 500-506 (in Chinese with English abstract).
[63]	许慧慧, 刘肖娟, 王孟珂, 毕泉鑫, 王利兵, 于海燕. 文冠果不同种质资源的叶片解剖结构分析及抗旱性评价. 浙江农林大学学报, 2023, 40: 348-355.
	Xu H H, Liu X J, Wang M K, Bi Q X, Wang L B, Yu H Y. Leaf anatomical structure and evaluation of drought resistance of different germplasm resources of Xanthoceras sorbifolium. J Zhejiang A&F Univ, 2023, 40: 348-355 (in Chinese with English abstract).
[64]	朱婧. 不同水分条件下6种园林灌木的生理反应研究. 河北农业大学硕士学位论文, 河北保定, 2022.
	Zhu J. Study on Physiological Responses under Different Water Conditions of Six Kinds Garden Shrubs. MS Thesis of Hebei Agricultural University, Baoding, Hebei, China, 2022 (in Chinese with English abstract).

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名称 Name	株高 Plant height (cm)	主茎粗 Stem diameter (mm)	地上鲜重 Aboveground fresh weight (kg)	鲜薯个数 Number of fresh cassava	单株产量 Yield per plant (kg)	鲜薯内皮/外皮/薯肉颜色 Cassava inner skin color/exterior skin color/flesh color
BJ	220.80±10.71 a	24.18±1.30 a	1.70±0.55 a	10.0±2.36 a	1.54±0.69 a	白色/红褐色/白色 White/reddish brown/white
ZY	278.60±16.57 b	28.16±4.20 a	2.46±0.71 a	12.2±3.27 a	2.66±0.73 b	白色/红褐色/白色 White/reddish brown/white
JY	257.40±11.85 c	24.06±4.96 a	1.92±0.61 a	10.0±3.32 a	2.02±0.31 ab	白色/红褐色/白色 White/reddish brown/white

样品名 Sample name	原始Reads总数 Total number of original Reads	碱基总数 Base total	过滤后Reads总数 Total number of Reads after filtering (%)	比对上参考基因组的Reads总数 Total number of Reads for the reference genome on the comparison (%)	Phred>20的百分比Q20 (%)	Phred>30的百分比Q30 (%)
BJ1	48,631,342	7,294,701,300	42,424,940 (87.24%)	39,417,519 (92.91%)	97.98	94.44
BJ2	48,954,268	7,343,140,200	43,028,526 (87.9%)	40,024,972 (93.02%)	97.88	94.15
BJ3	46,016,900	6,902,535,000	40,829,784 (88.73%)	37,826,541 (92.64%)	98.03	94.51
JY1	56,255,460	8,438,319,000	49,247,380 (87.54%)	46,094,300 (93.60%)	98.01	94.48
JY2	48,692,404	7,303,860,600	43,869,232 (90.09%)	40,496,556 (92.31%)	97.96	94.37
JY3	53,605,768	8,040,865,200	47,008,274 (87.69%)	43,423,863 (92.37%)	98.01	94.49
ZY1	55,114,316	8,267,147,400	48,942,778 (88.80%)	45,888,649 (93.76%)	97.96	94.37
ZY2	48,301,582	7,245,237,300	41,856,280 (86.66%)	39,058,323 (93.32%)	97.92	94.30
ZY3	50,036,022	7,505,403,300	44,199,486 (88.34%)	40,576,149 (91.80%)	97.90	94.26

突变类型 Mutation type	ZY突变基因数量 Number of ZY mutated genes	占ZY突变基因数量 Percentage of the number of ZY mutated genes (%)	BJ突变基因数量 Number of BJ mutated genes	占BJ突变基因数量 Percentage of the number of BJ mutated genes (%)	JY突变基因数量 Number of JY mutated genes	占JY突变基因数量 Percentage of the number of JY mutated genes (%)
同义单核苷酸突变Synonymous SNV	43,071	51.32	43,602	51.26	44,439	50.89
非同义单核苷酸突变 Non synonymous SNV	39,678	47.28	40,291	47.37	41,641	47.69
非移码替换 Non frameshift substitution	591	0.70	587	0.69	608	0.70
未知类型 Unknown	194	0.23	187	0.22	233	0.27
移码替换 Frameshift substitution	170	0.20	172	0.20	182	0.21
终止密码子增加 Stopgain	167	0.20	164	0.19	171	0.20

路径ID Pathway ID	条目 Term	数量Number
路径ID Pathway ID	条目 Term	ZY vs BJ	JY vs ZY
mesc00360	苯丙氨酸代谢 Phenylalanine metabolism	2	6
mesc00460	氰胺酸代谢 Cyanoamino acid metabolism	3	8
mesc00480	谷胱甘肽代谢 Glutathione metabolism	3	7
mesc00500	淀粉和蔗糖代谢 Starch and sucrose metabolism	7	12
mesc00592	α-亚麻酸代谢 Alpha-Linolenic acid metabolism	5	7
mesc00904	二萜类化合物的生物合成 Diterpenoid biosynthesis	2	5
mesc00940	苯丙素类化合物的生物合成 Phenylpropanoid biosynthesis	9	22
mesc00941	类黄酮的生物合成 Flavonoid biosynthesis	2	6
mesc04075	植物激素信号转导 Plant hormone signal transduction	5	12

基因ID	基因长度 Gene length	上调出现频数 Frequency of gene up-regulation	下调出现频数Frequency of gene downregulation	描述 Description	条目 Term
LOC110625143	1706	3	3	β-葡萄糖苷酶12 [木薯] XP_021626382.1 beta-glucosidase 12-like [Manihot esculenta]	苯丙素类化合物的生物合成 Phenylpropanoid biosynthesis α-亚麻酸代谢 Starch and sucrose metabolism 谷胱甘肽代谢 Cyanoamino acid metabolism
LOC110619850	1880	3	3	木薯亚麻苦甙水解酶 AAB22162.1 linamarase [Manihot esculenta]
LOC122724051	1849	3	3	木薯亚麻苦甙水解酶 AAB22162.1 linamarase [Manihot esculenta]
LOC110603741	1879	3	0	β-葡萄糖苷酶45 [木薯] XP_021597319.1 beta-glucosidase 45 [Manihot esculenta]
LOC110618460	1829	3	0	β-葡萄糖苷酶24-样异构体 X2 [木薯] XP_021617285.1 beta-glucosidase 24-like isoform X2 [Manihot esculenta]
LOC110618773	1768	3	0	β-葡萄糖苷酶24-样异构体 X1 [木薯] XP_021617701.1 beta-glucosidase 24-like isoform X1 [Manihot esculenta]
LOC110615840	2048	0	3	β-葡萄糖苷酶11 [木薯] XP_021613689.1 beta-glucosidase 11-like [Manihot esculenta]
LOC110625014	2363	2	2	PLA:苯丙氨酸氨解酶G4 [木薯] PLA: phenylalanine ammonia-lyase G4-like [Manihot esculenta]	苯丙氨酸代谢 Phenylalanine metabolism 类黄酮的生物合成 Flavonoid biosynthesis
LOC110618581	1657	2	2	肌酸合成酶[木薯] XP_021617435.1 vinorine synthase-like [Manihot esculenta]

卷叶木薯及其突变体叶片的比较转录组分析

Comparative transcriptome profiling of leaf in curled-leaf cassava and its mutant

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