作物学报 ›› 2024, Vol. 50 ›› Issue (9): 2297-2309.doi: 10.3724/SP.J.1006.2024.34193
QI Jia-Min1,*(), XU Chun-Miao2, XIAO Bin2
摘要:
TIFY家族是植物所特有的包含保守TIFY结构域(TIF [F/Y]XG)的转录因子基因家族, 在调节植物发育、响应非生物胁迫和植物激素方面具有重要作用。本研究在马铃薯(Solanum tuberosum L.)全基因组水平共鉴定出了26个StTIFY家族成员, 根据系统发育特征和蛋白结构将StTIFY分为4大亚家族, 其中JAZ亚家族分为了5个亚组。串联重复和片段重复事件在马铃薯TIFY基因家族的扩张中起主要作用。通过共线性分析发现, StTIFY与双子叶植物番茄(Solanum lycopersicum)、拟南芥(Arabidopsis)、甘蓝(Brassica oleracea)分别有21对、5对和6对直系同源基因, 而和单子叶植物水稻(Oryza sativa)、玉米(Zea mays)则分别只有1对和3对直系同源基因, 这些基因均在纯化选择下进化。RNA-seq数据分析发现, TIFY基因和ZML亚家族的2个基因在13个组织部位均有较高的表达量, 而其余成员则呈现组织表达特异性。同时我们进一步分析了StTIFY基因对非生物胁迫以及激素处理的响应, 发现有19个基因响应非生物胁迫(干旱/盐/热), 其中分别有3个和2个基因同时响应3种和2种非生物胁迫; 有5个基因在BAP、ABA、IAA和GA3处理下差异表达。利用qPCR进一步检测了在干旱胁迫条件下抗旱品种和水分敏感品种中4个候选基因的表达量, 结果发现这4个基因在2个品种的落花期显著差异表达。本研究为了解StTIFY基因家族的特征, 深入分析StTIFY家族成员在马铃薯响应激素信号和抵御非生物胁迫的功能提供了理论依据。
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