作物学报 ›› 2024, Vol. 50 ›› Issue (8): 1934-1947.doi: 10.3724/SP.J.1006.2024.32046
何丹丹1,2(), 舒亚洲1,2(), 周海连1,2, 吴松果1,2, 魏晓双1,2, 杨明冲1,2, 李波1,2, 吴正丹1,2, 韩世健2, 杨娟2,3, 王继斌1,2,4,*(), 王令强1,2,3,*()
HE Dan-Dan1,2(), SHU Ya-Zhou1,2(), ZHOU Hai-Lian1,2, WU Song-Guo1,2, WEI Xiao-Shuang1,2, YANG Ming-Chong1,2, LI Bo1,2, WU Zheng-Dan1,2, HAN Shi-Jian2, YANG Juan2,3, WANG Ji-Bin1,2,4,*(), WANG Ling-Qiang1,2,3,*()
摘要:
RPTA (regulatory particle triple-A ATPase)家族与植物的生长发育、激素调控和逆境胁迫反应密切相关。本研究一共鉴定到33个OsRPTA基因家族成员, 并分析了其基因位置、基因结构、motif组成和启动子顺式作用元件等信息。随后, 利用水稻CREP数据库下载的数据, 分析了OsRPTA基因家族成员的全生育期组织表达模式。发现大部分OsRPTA基因在穗、胚乳和愈伤组织中具有较高的表达水平。β-D-葡萄糖苷酸酶(GUS)染色进一步显示, 基因成员OsRPTA18主要在叶枕、根、叶、叶鞘、茎节、内稃和外稃的维管束等部位表达。亚细胞定位结果显示OsRPTA18蛋白定位于细胞核。通过CRISPR/Cas9基因编辑获得了突变体材料osrpta18-1和osrpta18-2。与中花11相比, 突变体植株的株高、叶倾角变小, 粒宽和千粒重降低。组织切片结果表明, 突变体osrpta18旗叶倾角变小是由于叶枕近轴面厚壁细胞增殖, 导致近轴面与远轴面细胞和维管束的不对称性减弱。本研究有助于了解水稻RPTA基因家族功能, 并为利用OsRPTA18基因培育理想株型的水稻品种提供参考。
[1] | Chen L T, Li Y C, Sottas C, Lazaris A, Petrillo S K, Metrakos P, Li L, Ishida Y, Saito T, Garza S, Papadopoulos V. Loss of mitochondrial ATPase ATAD3A contributes to nonalcoholic fatty liver disease through accumulation of lipids and damaged mitochondria. J Biol Chem, 2022, 298: 102008. |
[2] |
Xu H Y, Holly V R. The SarcoEndoplasmic Reticulum Calcium ATPase (SERCA) pump: a potential target for intervention in aging and skeletal muscle pathologies. Skelet Muscle, 2021, 11: 25.
doi: 10.1186/s13395-021-00280-7 pmid: 34772465 |
[3] |
Kotani Y, Morito D, Yamazaki S, Ogino K, Kawakami K, Takashima S, Hirata H, Nagata K. Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213. Sci Rep, 2015, 5: 16161.
doi: 10.1038/srep16161 pmid: 26530008 |
[4] |
Morito D, Nishikawa K, Hoseki J, Kitamura A, Kotani Y, Kiso K, Kinjo M, Fujiyoshi Y, Nagata K. Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state. Sci Rep, 2014, 4: 4442.
doi: 10.1038/srep04442 pmid: 24658080 |
[5] |
Qi Y, Liu H, Daniels M P, Zhang G, Xu H. Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration. Cell Death Differ, 2016, 23: 291-302.
doi: 10.1038/cdd.2015.94 pmid: 26160069 |
[6] | Cui K, Qin L, Tang X Y, Nong J K, Chen J, Wu N, Gong X, Yi L X, Yang C H, Xia S T. A single amino acid substitution in RFC4 leads to endoduplication and compromised resistance to DNA damage in Arabidopsis thaliana. Genes, 2022, 13: 1037. |
[7] | Zhang B T, Van Aken O, Thatcher L, De Clercq I, Duncan O, Law S R, Murcha M W, van der Merwe M, Seifi H S, Carrie C, Cazzonelli C, Radomiljac J, Höfte M, Singh K B, Van Breusegem F, Whelan J. The mitochondrial outer membrane AAA ATPase AtOM66 affects cell death and pathogen resistance in Arabidopsis thaliana. Plant J, 2014, 80: 709-727. |
[8] |
Wu Q F, Han T T, Yang L, Wang Q, Zhao Y X, Jiang D, Ruan X. The essential roles of OsFtsH2 in developing the chloroplast of rice. BMC Plant Biol, 2021, 21: 445.
doi: 10.1186/s12870-021-03222-z pmid: 34598671 |
[9] | 张海丽, 高静, 张昊, 李生辉, 邢继红, 王凤茹, 董金皋. 油菜素内酯对水稻细胞伸长和分裂的调控. 农业生物技术学报, 2015, 23: 71-79. |
Zhang H L, Gao J, Zhang H, Li S H, Xing J H, Wang F R, Dong J G. The regulation of brassinosteroid (BR) on elongation and division of rice (Oryza sativa) cells. J Agric Biotechnol, 2015, 23: 71-79 (in Chinese with English abstract). | |
[10] | Guo J F, Li W, Shang L G, Wang Y G, Yan P, Bai Y H, Da X W, Wang K, Guo Q Q, Jiang R R, Mao C Z, Mo X R. OsbHLH98 regulates leaf angle in rice through transcriptional repression of OsBUL1. New Phytol, 2021, 230: 1953-1966. |
[11] | Huang Q N, Shi Y F, Zhang X B, Song L X, Feng B H, Wang H M, Xu X, Li X H, Guo D, Wu J L. Single base substitution in OsCDC48isresponsible for premature senescence and death phenotype in rice. J Integr Plant Biol, 2016, 58: 12-28. |
[12] | Zhang Y, Iqbal M F, Wang Y L, Qian K Y, Xiang J X, Xu G H, Fan X R. OsTBP2.1, A TATA-binding protein, alters the ratio of OsNRT2.3b to OsNRT2.3a and improves rice grain yield. Int J Mol Sci, 2022, 23: 10795. |
[13] | Yedidi R S, Wendler P, Enenkel C. AAA-ATPases in protein degradation. Int J Mol Sci, 2017, 4: 42. |
[14] |
Tong H N, Chu C C. Functional specificities of brassinosteroid and potential utilization for crop improvement. Trends Plant Sci, 2018, 23: 1016-1028.
doi: S1360-1385(18)30187-0 pmid: 30220494 |
[15] |
许娜, 徐铨, 徐正进, 陈温福. 水稻株型生理生态与遗传基础研究进展. 作物学报, 2023, 49: 1735-1746.
doi: 10.3724/SP.J.1006.2023.22050 |
Xu N, Xu Q, Xu Z J, Chen W F. Research progress on physiological ecology and genetic basis of rice plant architecture. Acta Agron Sin, 2023, 49: 1735-1746 (in Chinese with English abstract).
doi: 10.3724/SP.J.1006.2023.22050 |
|
[16] |
马梦影, 巩文靓, 康雪蒙, 段海燕. 水稻理想株型改良的研究进展. 中国农学通报, 2020, 36(29): 1-6.
doi: 10.11924/j.issn.1000-6850.casb20190900610 |
Ma M Y, Gong W Y, Kang X M, Duan H Y. The improvement of ideal plant type of rice: a review. Chin Agric Sci Bull, 2020, 36(29): 1-6 (in Chinese with English abstract).
doi: 10.11924/j.issn.1000-6850.casb20190900610 |
|
[17] | Dong H J, Zhao H, Li S L, Han Z M, Hu G, Liu C, Yang G Y, Wang G W, Xie W B, Xing Y Z. Genome-wide association studies reveal that members of bHLH subfamily 16 share a conserved function in regulating flag leaf angle in rice (Oryza sativa L.). PLoS Genet, 2018, 14: 1007323. |
[18] | 徐静, 王莉, 钱前, 张光恒. 水稻叶片形态建成分子调控机制研究进展. 作物学报, 2013, 39: 767-774. |
Xu J, Wang L, Qian Q, Zhang G H. Research advance in molecule regulation mechanism of leaf morphogenesis in rice (Oryza sativa L.). Acta Agron Sin, 2013, 39: 767-774 (in Chinese with English abstract). | |
[19] |
胡娟, 林晗, 徐娜, 焦然, 戴志俊, 鲁草林, 饶玉春, 王跃星. 水稻叶倾角分子机制及育种应用的研究进展. 中国水稻科学, 2019, 33: 391-400.
doi: 10.16819/j.1001-7216.2019.9029 |
Hu J, Lin H, Xu N, Jiao R, Dai Z J, Lu C L, Rao Y C, Wang Y X. Advances in molecular mechanisms of rice leaf inclination and its application in breeding. Chin J Rice Sci, 2019, 33: 391-400 (in Chinese with English abstract).
doi: 10.16819/j.1001-7216.2019.9029 |
|
[20] | Huang P, Zhao J Z, Hong J L, Zhu B, Xia S, Zhu E G, Han P F, Zhang K W. Cytokinins regulate rice lamina joint development and leaf angle. Plant Physiol, 2023, 191: 56-69. |
[21] | Xing M Q, Wang W, Fang X, Xue H W. Rice OsIAA6 interacts with OsARF1 and regulates leaf inclination. Crop J, 2022, 10: 1580-1588. |
[22] |
Fàbregas N, Caño-Delgado A I. Turning on the microscope turret: a new view for the study of brassinosteroid signaling in plant development. Physiol Plant, 2014, 151: 172-183.
doi: 10.1111/ppl.12130 pmid: 24547704 |
[23] | 张晓琼, 王晓雯, 田维江, 张孝波, 孙莹, 李杨羊, 谢佳, 何光华, 桑贤春. LAZY1通过BR途径调控水稻叶夹角的发育. 作物学报, 2017, 43: 1767-1773. |
Zhang X Q, Wang X W, Tian W J, Zhang X B, Sun Y, Li Y Y, Xie J, He G H, Sang X C. LAZY1regulates the development of rice leaf angle through BR pathway. Acta Agron Sin, 2017, 43: 1767-1773 (in Chinese with English abstract). | |
[24] |
Gan L J, Wu H, Wu D P, Zhang Z F, Guo Z F, Yang N, Xia K, Zhou X, Oh K, Matsuoka M, Ng D, Zhu C H. Methyl jasmonate inhibits lamina joint inclination by repressing brassinosteroid biosynthesis and signaling in rice. Plant Sci, 2015, 241: 238-245.
doi: 10.1016/j.plantsci.2015.10.012 pmid: 26706074 |
[25] | Chen H J, Wan Y, Teng K C, Liu B H, Zhao N, Xu K Z, Li J X. The role of OsOFP8gene in regulating rice leaf angle. J Plant Biochem Biot, 2023, 32: 304-318. |
[26] | 凌启鸿, 陆卫平, 蔡建中, 曹显祖. 水稻根系分布与叶角关系的研究初报. 作物学报, 1989, 15: 123-131. |
Ling Q H, Lu W P, Cai J Z, Cao X Z. The relationship between root distribution and leaf angle in rice plant. Acta Agron Sin, 1989, 15: 123-131 (in Chinese with English abstract). | |
[27] |
Liu J M, Park S J, Huang J, Lee E J, Xuan Y H, Je BI, Kumar V, Priatama R A, Vimal R K, Kim S H, Min M K, Cho J H, Kim T H, Chandran A K N, Jung K H, Takatsuto S, Fujioka S, Han C D. Loose Plant Architecture1 (LPA1) determines lamina joint bending by suppressing auxin signalling that interacts with C-22-hydroxylated and 6-deoxo brassinosteroids in rice. J Exp Bot, 2016, 67: 1883-1895.
doi: 10.1093/jxb/erw002 pmid: 26826218 |
[28] |
Luo X Y, Zheng J S, Huang R Y, Huang Y M, Wang H C, Jiang L R, Fang X J. Phytohormones signaling and crosstalk regulating leaf angle in rice. Plant Cell Rep, 2016, 35: 2423-2433.
pmid: 27623811 |
[29] | Kokkirala V R, Yonggang P, Abbagani S, Zhu Z, Umate P. Subcellular localization of proteins of Oryza sativa L. in the model tobacco and tomato plants. Plant Signal Behav, 2010, 5: 1336-1341. |
[30] | Lee J H, Schöffl F. GUS activity staining in gels: a powerful tool for studying protein interactions in plants. Plant Mol Biol Rep, 1995, 13: 346-354. |
[31] | 杨捷频. 常规石蜡切片方法的改良. 生物学杂志, 2006, 23(1): 45-46. |
Yang J P. Improvement of traditional paraffin section preparation methods. J Biol, 2006, 23(1): 45-46 (in Chinese with English abstract). | |
[32] |
Ferrero-Serrano Á, Assmann S M. The α-subunit of the rice heterotrimeric G protein, RGA1, regulates drought tolerance during the vegetative phase in the dwarf rice mutant d1. J Exp Bot, 2016, 67: 3433-3443.
doi: 10.1093/jxb/erw183 pmid: 27194741 |
[33] |
Cao Y Y, Zhong Z J, Wang H Y, Shen R X. Leaf angle: a target of genetic improvement in cereal crops tailored for high-density planting. Plant Biotechnol J, 2022, 20: 426-436.
doi: 10.1111/pbi.13780 pmid: 35075761 |
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