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作物学报

作物学报 ›› 2025, Vol. 51 ›› Issue (1): 44-57.doi: 10.3724/SP.J.1006.2025.44079

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

甘蓝型油菜BnaSLY1基因进化分析及功能研究

李嘉欣(), 黄莹, 吴潞梅, 赵伦, 易斌, 马朝芝, 涂金星, 沈金雄, 傅廷栋, 文静*()   

  1. 华中农业大学作物遗传改良全国重点实验室 / 国家油菜工程技术研究中心 / 洪山实验室, 湖北武汉 430070
  • 收稿日期:2024-05-13 接受日期:2024-08-15 出版日期:2025-01-12 网络出版日期:2024-09-02
  • 通讯作者: *文静, E-mail: wenjing@mail.hzau.edu.cn
  • 作者简介:E-mail: 18174003505@163.com
  • 基金资助:
    国家自然科学基金项目(31771831);国家自然科学基金项目(31000721);财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-12)

Phylogenetic and functional analysis of the BnaSLY1 genes in Brassica napus L.

LI Jia-Xin(), HUANG Ying-Ying, WU Lu-Mei, ZHAO Lun, YI Bin, MA Chao-Zhi, TU Jin-Xing, SHEN Jin-Xiong, FU Ting-Dong, WEN Jing*()   

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University / National Engineering Research Center of Rapeseed / Hongshan Laboratory, Wuhan 430070, Hubei, China
  • Received:2024-05-13 Accepted:2024-08-15 Published:2025-01-12 Published online:2024-09-02
  • Contact: *E-mail: wenjing@mail.hzau.edu.cn
  • Supported by:
    National Natural Science Foundation of China(31771831);National Natural Science Foundation of China(31000721);China Agriculture Research System of MOF and MARA(CARS-12)

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摘要：

赤霉素调控植物表皮细胞增长、茎叶伸长以及株型建成。拟南芥SLY1属于F-box蛋白, 它通过靶向泛素化赤霉素信号转导路径的负向调控因子——DELLA蛋白来影响植物生长发育, 然而油菜中BnaSLY1的基因功能尚未揭示。本研究对BnaSLY1进行了表达特征和进化树分析, 利用CRISPR/Cas9技术创制了BnaSLY1不同拷贝数的突变体, 结合RNA-Seq技术对BnaSLY1的生物学功能及其对油菜生长发育的影响进行了研究。结果表明, 甘蓝型油菜Westar中有2个SLY1同源拷贝BnaA01.SLY1和BnaA06.SLY1, 它们表达模式基本相同, 为组成型表达基因, 其蛋白定位在细胞核, 且在不同的油菜品种及十字花科植物间序列保守。与对照相比, 单突bnaa01sly1和bnaa06sly1开花时间推迟, 株高显著降低, 而双突bnasly1还表现出深绿色光叶表型, 叶片厚度增加, 开花期比单突进一步推迟, 株高也进一步降低。RNA-Seq结果显示, 双突与Westar之间的差异表达基因显著富集在生长素信号转导路径以及蜡质合成通路, 多个开花时间相关基因表达也发生显著变化。本研究表明, BnaSLY1除影响植物株高和开花时间等生长发育进程, 还影响表皮蜡质合成, 为探索赤霉素信号转导路径在甘蓝型油菜生长发育中的重要作用奠定了理论基础。

关键词: 甘蓝型油菜, BnaSLY1, 赤霉素, 株型, 蜡质合成, 开花时间, RNA-Seq

Abstract:

Gibberellins regulate plant epidermal cell growth, stem and leaf expansion, and plant architecture. In Arabidopsis, SLY1 encodes an F-box protein that modulates plant growth by targeting the negative regulator of GA signaling, the DELLA protein, for ubiquitination and subsequent degradation. However, the function of BnaSLY1 in Brassica napus has not been previously revealed. In this study, we characterized the expression patterns and performed a phylogenetic analysis of BnaSLY1. Using CRISPR/Cas9 technology, we generated mutants with different copy numbers of BnaSLY1. By integrating RNA-Seq analysis, we investigated the biological functions of BnaSLY1 and its impact on the growth and development of Brassica napus. Our results showed that there are two copies of SLY1 in Brassica napus, with similar expression patterns and constitutive expression. The protein is localized in the nucleus and is highly conserved among different varieties of rapeseed and cruciferous plants. Phenotypic analysis of mutants revealed that, compared to the control, single mutants bnaa01sly1 and bnaa06sly1 exhibited delayed flowering and significantly reduced plant height, while the double mutant bnasly1 showed a dark green leaf phenotype, increased leaf thickness, further delayed flowering, and further reduced plant height. RNA-Seq analysis between Westar and bnasly1 showed significant enrichment of differentially expressed genes in the auxin signaling pathway and wax biosynthesis pathway, with several flowering time-related genes showing significant expression changes. This study demonstrates that BnaSLY1 not only influences plant height and flowering time but also affects epidermal wax synthesis, thereby laying a theoretical foundation for exploring the crucial role of the GA signaling pathway in the growth and development of Brasscia napus.

Key words: Brassica napus L., BnaSLY1, gibberellin, plant architecture, wax biosynthesis, flowering time, RNA-seq

附表1

本研究用到的引物"

用途
Functions		 引物
Primers		 序列
Sequence (5'-3')
qRT-PCR分析
qRT-PCR Analysis		 QBnaSLY1.A01-F TCAAACTCAGTTACAAACGGTGACG
QBnaSLY1.A01-R TGCTCACGCAAGAAGACGTTGCTAA
QBnaSLY1.A06-F TCAAACTCAGTTACAGACGGT
QBnaSLY1.A06-R GCAAGATGATGTTGCTAAGG
QActin-F GCTGACCGTATGAGCAAAG
QActin-R AAGATGGATGGACCCGAC
亚细胞定位
Subcellular localization		 C83R TGTGCCCATTAACATCACCATC
SalI-GFP-F CTTGCATGCCTGCAGGTCGACATGAAACGCAGTGCTTCAAACT
BamHI-GFP-R TCTACCGGTACCCGGGGATCCCCGGGGAGTCTCTTAGTGAAATTCATC
CRISPR/Cas9基因
编辑
CRISPR/Cas9
gene editing		 Cr-SLY1.A01-F0 ATATATGGTCTCGATTGGAGTAGATTCTCGTCTAAGTGTT
Cr-SLY1.A01-R0 TGTACTAGGTGCGGAAGCACGGTTTTAGAGCTAGAAATAGC
Cr-SLY1.A01-BsF AACGAGTAGATTCTCGTCTAAGTCAATCTCTTAGTCGACTCTAC
Cr-SLY1.A01-BsR ATTATTGGTCTCGAAACTACTAGGTGCGGAAGCACGCAA
Cr-SLY1.A06-F0 ATATATGGTCTCGATTGCCAGCTCAGATTCGTAGTCTGTT
Cr-SLY1.A06-R0 TGAGACTACGAATCTGAGCTGGGTTTTAGAGCTAGAAATAGC
Cr-SLY1.A06-BsF AACCCAGCTCAGATTCGTAGTCTCAATCTCTTAGTCGACTCTAC
Cr-SLY1.A06-BsR ATTATTGGTCTCGAAACAGACTACGAATCTGAGCTGGCAA
Cr-SLY1s-F0 ATATATGGTCTCGATTGCGTGAGCAAGATCTGGCACGTT
Cr-SLY1s-R0 TGCGTGCTTCAACACCTCGTAGTTTTAGAGCTAGAAATAGC
Cr-SLY1s-BsF AACCGTGAGCAAGATCTGGCACCAATCTCTTAGTCGACTCTAC
Cr-SLY1s-BsR ATTATTGGTCTCCGTGCTTCAACACCTCGTACAA
阳性苗检测
Detection of positive transgenic plants		 U626-IDF TGTCCCAGGATTAGAATGATTAGGC
U629-IDR AGCCCTCTTCTTTCGATCCATCAAC
Hi-TOM分析
Hi-Tom analysis		 A01-T1/T2-F GGAGTGAGTACGGTGTGCCAAGAAGATGAAAAAGACCACAGA
A01-T1/T2-R GAGTTGGATGCTGGATGGTAGAGCGAGTGGAGTTGCTTGAA
A06-T1/T2-F GGAGTGAGTACGGTGTGCTGACGCGAGTAACAAGAAGATG
A06-T1/T2-R GAGTTGGATGCTGGATGGTCGAGAGAGGCCACAGGTA
HI-T1/T2-F GGAGTGAGTACGGTGTGCAAGATCTGGCACAGGACT
HI-T1/T2-R GAGTTGGATGCTGGATGGTAGTACCGAATCGAGAGGAG
F-1 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTGCGTTGGAGTGAGTACGGTGTGC
F-2 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTGTAGTGGAGTGAGTACGGTGTGC
F-3 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTACGCTGGAGTGAGTACGGTGTGC
F-4 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTCTCGTGGAGTGAGTACGGTGTGC
F-5 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTGCTCTGGAGTGAGTACGGTGTGC
F-6 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTAGTCTGGAGTGAGTACGGTGTGC
F-7 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTCGACTGGAGTGAGTACGGTGTGC
F-8 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTGATGTGGAGTGAGTACGGTGTGC
F-9 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTATACTGGAGTGAGTACGGTGTGC
F-10 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTCACATGGAGTGAGTACGGTGTGC
F-11 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTGTGCTGGAGTGAGTACGGTGTGC
F-12 ACTCTTTCCCTACACGACGCTCTTCCGATCTGCTTACTATGGAGTGAGTACGGTGTGC
R-A GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTGCGTTGAGTTGGATGCTGGATGG
R-B GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTGTAGTGAGTTGGATGCTGGATGG
R-C GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTACGCTGAGTTGGATGCTGGATGG
R-D GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTCTCGTGAGTTGGATGCTGGATGG
R-E GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTGCTCTGAGTTGGATGCTGGATGG
R-F GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTAGTCTGAGTTGGATGCTGGATGG
R-G GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTCGACTGAGTTGGATGCTGGATGG
R-H GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCTGTGATGTGAGTTGGATGCTGGATGG
indexP-F11 AATGATACGGCGACCACCGAGATCTACACTCATGGTCACACTCTTTCCCTACACGACGC
indexP-F12 AATGATACGGCGACCACCGAGATCTACACAGCGATGTACACTCTTTCCCTACACGACGC
indexP-R17 CAAGCAGAAGACGGCATACGAGATTTACGAGGGTGACTGGAGTTCAGACGTGTGCTCTT
indexP-R18 CAAGCAGAAGACGGCATACGAGATACAGTGTGGTGACTGGAGTTCAGACGTGTGCTCTT

附图1

不同甘蓝型油菜品种间SLY1核苷酸序列比对 A: 不同品种间A亚基因组SLY1核苷酸序列比对; B: 不同品种间C亚基因组SLY1核苷酸序列比对。黑色阴影部分为外显子，灰色部分为内含子。"

图1

BnaSLY1的蛋白序列和SLY1进化分析 A: 拟南芥、白菜、甘蓝和甘蓝型油菜(Westar)中SLY1氨基酸序列比较分析; B: 不同甘蓝型油菜品种间BnaSLY1进化树分析, 分支上数字代表bootstrap值(%); C: 不同物种SLY1进化树分析。绿色部分代表I群, 蓝色部分代表II群, 其余颜色代表III群。CICLE: 柑橘; FCD: 无花果; EVM: 大麻; Cla97: 西瓜; SORBI: 高粱; Zm: 玉米; Os: 水稻; Traes: 小麦; HORVU: 大麦; AVESA: 燕麦; Rchi: 蔷薇; Csa: 黄瓜; Vitvi: 葡萄; EUTSA: 盐芥; DCAR: 胡萝卜; CEY00: 猕猴桃; SIN: 芝麻; HanXRQ: 向日葵; LSAT: 莴苣; FRAEX: 白蜡; OE9A: 橄榄; AT: 拟南芥; Bna: 甘蓝型油菜; Bra: 白菜; Bo: 甘蓝。"

图2

BnaSLY1表达特征 A: 不同拷贝BnaSLY1在Westar各组织中的相对表达量; B: BnaSLY1在ZS11各组织中的表达热图, 表达数据为各基因TPM值, 数据来源于BnIR数据库; C: BnaSLY1在本氏烟草中的亚细胞定位。标尺为25 μm。"

图3

BnaSLY1靶点设计及基因敲除突变体编辑情况 A: BnaSLY1基因结构和靶点位置; B: T1代BnaSLY1基因靶点突变情况。绿色字体表示PAM序列, 红色字体表示突变碱基, 红色虚线表示碱基缺失。"

附表2

CRISPR/Cas9敲除转基因T0代阳性苗靶点编辑情况统计"

突变体
Mutant		 再生植株
Number of
transgenic plants		 阳性株数
Number of positive plants		 编辑效率
Edit efficiency (%)		 拷贝全突
Total mutation efficiency (%)		 靶点1编辑效率
Editing efficiency of target 1 (%)		 靶点2编辑效率
Editing efficiency of target 2 (%)
bnaa01sly1 22 18 (81.82%) 66.70 41.70 58.30 33.30
bnaa06sly1 10 7 (70.00%) 57.10 25.00 100.00 0
bnasly1 86 65 (75.58%) 64.60 28.60 52.30 69.10

图4

BnaSLY1基因敲除突变体表型特征 A: Westar和突变体叶片表型; B: Westar和突变体苗期表型; C: Westar和突变体花期表型; D: Westar和突变体株高; E: Westar和突变体开花时间。标尺: 8 cm。**表示差异极显著(P < 0.01)。"

图5

BnaSLY1影响叶片细胞形态、叶绿素及表皮蜡质合成 A: WT和突变体的叶片石蜡切片, 标尺为100 μm; B: WT和突变体叶绿体超微结构, 标尺为2 μm; C: WT和突变体叶片厚度统计; D: WT和突变体叶绿素含量比较。sm: 海绵组织; Cp: 叶绿体; Tm: 类囊体。*和**分别表示差异显著(P < 0.05)和极显著(P < 0.01); E: WT和突变体叶片表皮蜡质扫描电镜; 标尺为5 μm。"

图6

Westar与bnasly1内源GA含量比较 **表示差异极显著(P < 0.01)。"

附表3

样品RNA-seq测序数据总览"

样本
Sample		 测序总读数
Total reads		 质控后读数
Clean reads		 错误率
Error rate (%)		 总比对率
Mapped ratio (%)		 Q20 Q30
(%) (%)
CK1 51,374,928 50,456,150 0.026053 94.82 97.16 94.89
CK2 44,454,300 43,654,324 0.026104 94.73 97.18 94.92
CK3 46,223,656 45,461,716 0.002838 94.86 97.25 95.02
T1 43,448,334 42,710,898 0.002819 94.73 97.26 95.04
T2 41,761,542 41,089,858 0.002842 94.78 97.28 95.06
T3 41,721,250 40,921,468 0.008204 94.58 97.06 94.7

附图2

转录组样品皮尔森相关系数"

附图3

Westar与bnasly1差异基因火山图"

附图4

Westar与bnasly1差异基因GO分类"

附图5

Westar与bnasly1差异基因KEGG富集分析"

图7

生长素信号转导通路差异表达基因分析 a、b、c表示基因在Westar三个生物学重复中的log10(FPKM)值, d、e、f表示基因在bnasly1三个生物学重复中log10(FPKM)值, g表示Westar与突变体之间的log2FC。AUX1/IAA: 生长素/吲哚-3-乙酸; TIR1: 转运抑制响应蛋白; ARF: 生长素响应因子; SAUR: 生长素上调小RNA; GH3: 酰胺合成酶。"

附表4

GA合成及信号转导路径差异基因"

Gene ID Putative gene FPKM log2Fold Change
WT-1 WT-2 WT-3 bnasly1-1 bnasly1-2 bnasly1-3
BnaA01G0149400ZS BnaA01.GA20ox1 1.91 2.61 2.32 9.73 7.20 10.23 2.06
BnaC01G0190000ZS BnaC01.GA20ox1 0.56 0.21 0.77 7.48 5.88 8.21 3.86
BnaC07G0465100ZS BnaC07.GA20ox1 0.00 0.00 0.10 2.27 2.26 3.63 6.44
BnaA06G0105000ZS BnaA06.GA3ox1 0.59 0.61 1.51 11.75 10.50 17.79 3.95
BnaC05G0130100ZS BnaC05.GA3ox1 1.22 1.12 1.57 8.35 8.45 10.71 2.89
BnaA02G0232000ZS BnaA02.GA2ox1 1.48 3.44 1.51 0.00 0.07 0.27 -4.16
BnaC06G0441000ZS BnaC06.GA2ox1 3.20 3.60 3.07 0.57 0.46 0.13 -3.02
BnaC05G0539100ZS BnaC05.GID1A 10.15 8.42 8.88 27.94 25.41 27.80 1.63
BnaA04G0003700ZS BnaA04.GID1B 0.88 0.17 0.49 2.02 2.18 1.27 1.90
BnaC04G0258300ZS BnaC04.GID1B 1.98 1.65 1.12 7.63 5.73 7.36 2.19
BnaC08G0417900ZS BnaC08.GID1B 3.28 3.63 3.36 6.70 7.75 7.95 1.20
BnaA02G0160500ZS BnaA02.RGL1 6.49 12.58 7.56 2.48 2.13 3.04 -1.72
BnaC02G0205300ZS BnaC02.RGL1 7.19 17.43 9.53 3.80 3.51 2.02 -1.80
Gene ID Regulation GO KEGG Swissprot Pfam NR
BnaA01G0149400ZS Up GO:0005506 K05282 Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2 non-haem dioxygenase in morphine synthesis N-terminal BnaA01g35230D [Brassica napus]
BnaC01G0190000ZS Up GO:0005506 K05282 Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2 non-haem dioxygenase in morphine synthesis N-terminal BnaC01g17380D [Brassica napus]
BnaC07G0465100ZS Up GO:0005506 K05282 Gibberellin 20 oxidase 1 OS=Arabidopsis thaliana GN=GA20OX1 PE=2 SV=2 2OG-Fe(II) oxygenase superfamily BnaC07g39650D [Brassica napus]
BnaA06G0105000ZS Up GO:0016491 K04124 Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2 non-haem dioxygenase in morphine synthesis N-terminal BnaA06g10250D [Brassica napus]
BnaC05G0130100ZS Up GO:0016491 K04124 Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA3OX1 PE=1 SV=2 non-haem dioxygenase in morphine synthesis N-terminal BnaC05g11920D [Brassica napus]
BnaA02G0232000ZS Down GO:0016491 K04125 Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2 non-haem dioxygenase in morphine synthesis N-terminal PREDICTED: gibberellin 2-beta-dioxygenase 1-like [Brassica rapa]
BnaC06G0441000ZS Down GO:0005506 K04125 Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana GN=GA2OX1 PE=2 SV=2 non-haem dioxygenase in morphine synthesis N-terminal BnaC06g38910D [Brassica napus]
BnaC05G0539100ZS Up GO:0008152 K14493 Gibberellin receptor GID1A OS=Arabidopsis thaliana GN=GID1A PE=1 SV=1 alpha/beta hydrolase fold BnaC05g46680D [Brassica napus]
BnaA04G0003700ZS Up GO:0008152 K14493 Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1 alpha/beta hydrolase fold BnaA04g00210D [Brassica napus]
BnaC04G0258300ZS Up GO:0008152 K14493 Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1 alpha/beta hydrolase fold BnaC04g21040D [Brassica napus]
BnaC08G0417900ZS Up GO:0008152 K14493 Gibberellin receptor GID1B OS=Arabidopsis thaliana GN=GID1B PE=1 SV=1 alpha/beta hydrolase fold BnaCnng55170D [Brassica napus]
BnaA02G0160500ZS Down — K14494 DELLA protein RGL1 OS=Arabidopsis thaliana GN=RGL1 PE=1 SV=1 Transcriptional regulator DELLA protein N terminal BnaCnng68300D [Brassica napus]
BnaC02G0205300ZS Down — K14494 DELLA protein RGL1 OS=Arabidopsis thaliana GN=RGL1 PE=1 SV=1 Transcriptional regulator DELLA protein N terminal BnaCnng28010D [Brassica napus]

图8

蜡质合成通路差异表达基因分析 a、b、c表示基因在Westar三个生物学重复中log10(FPKM)值, d、e、f表示基因在bnasly1三个生物学重复中log10(FPKM)值, g表示野生型与突变体之间的log2FC。LACS: 长链酰基辅酶A合成酶; FAE: 脂肪酸延伸酶; CER3: 脂肪酸羟化酶(FAE复合体亚基); CER26: 转氨酶(FAE复合体亚基); WSD1: 蜡酯合酶/二酰甘油酰基转移酶; FAR: 脂肪酰基辅酶A还原酶; MAH1: 中链烷烃羟化酶; CYP: 细胞色素P450; GPAT: 甘油-3-磷酸酰基转移酶; 2-MHG: 2-单(10,16)-二羟基十六烷酰基甘油。"

附表5

花期相关差异基因"

Gene ID Putative gene FPKM log2Fold Change
WT-1 WT-2 WT-3 bnasly1-1 bnasly1-2 bnasly1-3
BnaA09G0591400ZS BnaA09.SVP 33.92 22.94 37.72 72.61 70.84 70.94 1.25
BnaC04G0438900ZS BnaC04.SVP 24.46 12.02 17.51 43.86 41.38 35.38 1.22
BnaC08G0443200ZS BnaC08.SVP 17.09 9.5 20.32 41.15 35.3 39.69 1.37
BnaA03G0039200ZS BnaA03.FLC 11.26 15.3 18.45 33.08 34.89 33.12 1.22
BnaA08G0227500ZS BnaA08.TEM1 2.00 0.95 1.51 9.99 5.86 11.65 2.68
BnaA09G0438900ZS BnaA09.TEM1 2.92 1.77 1.66 5.94 4.19 9.05 1.66
BnaC03G0630000ZS BnaC03.TEM1 1.84 1.30 1.43 9.28 4.55 10.92 2.50
BnaC05G0224800ZS BnaC05.TEM1 1.69 1.29 0.62 4.9 4.20 6.85 2.20
BnaA01G0166300ZS BnaA01.SPL 7.43 6.46 6.02 0 0 0 -9.03
BnaC01G0213600ZS BnaC01.SPL 5.96 5.57 7.85 0.25 0 0 -6.13
BnaC07G0475500ZS BnaC07.SPL 3.13 4.25 4.11 0 0.16 0.08 -5.52
BnaA07G0282700ZS BnaA07.FT 2.92 4.78 2.68 0.45 0.20 0.61 -2.95
BnaC06G0323800ZS BnaC06.FT 9.78 13.22 17.44 1.18 0.86 2.72 -3.02
Gene ID Regulation GO KEGG Swissprot Pfam NR
BnaA09G0591400ZS Up GO:0003700 K09264 MADS-box protein SVP OS=Arabidopsis thaliana GN=SVP PE=1 SV=1 K-box region short vegetative phase protein [Brassica juncea]
BnaC04G0438900ZS Up GO:0003677 K09264 MADS-box protein SVP OS=Arabidopsis thaliana GN=SVP PE=1 SV=1 K-box region MADS-box protein SVP [Brassica napus]
BnaC08G0443200ZS Up GO:0003677 K09264 MADS-box protein SVP OS=Arabidopsis thaliana GN=SVP PE=1 SV=1 K-box region BnaC08g34920D [Brassica napus]
BnaA03G0039200ZS Up GO:0003677 K09264 MADS-box protein FLOWERING LOCUS C OS=Arabidopsis thaliana GN=FLC PE=2 SV=1 K-box region MADS-box protein [Brassica napus]
BnaA08G0227500ZS Up GO:0003677 K09287 AP2/ERF and B3 domain-containing transcription repressor TEM1 OS=Arabidopsis thaliana GN=TEM1 PE=1 SV=1 AP2 domain PREDICTED: AP2/ERF and B3 domain-containing transcription repressor TEM1-like [Brassica rapa]
BnaA09G0438900ZS Up GO:0003677 K09287 AP2/ERF and B3 domain-containing transcription repressor TEM1 OS=Arabidopsis thaliana GN=TEM1 PE=1 SV=1 AP2 domain PREDICTED: AP2/ERF and B3 domain-containing transcription repressor TEM1-like [Brassica rapa]
BnaC03G0630000ZS Up GO:0003677 K09287 AP2/ERF and B3 domain-containing transcription repressor TEM1 OS=Arabidopsis thaliana GN=TEM1 PE=1 SV=1 AP2 domain BnaCnng04580D [Brassica napus]
BnaC05G0224800ZS Up GO:0003677 K09287 AP2/ERF and B3 domain-containing transcription repressor TEM1 OS=Arabidopsis thaliana GN=TEM1 PE=1 SV=1 AP2 domain BnaC05g20560D [Brassica napus]
BnaA01G0166300ZS Down — — — Plant transcription factor NOZZLE BnaA01g16350D [Brassica napus]
BnaC01G0213600ZS Down — — — Plant transcription factor NOZZLE BnaC01g19500D [Brassica napus]
BnaC07G0475500ZS Down — — — Plant transcription factor NOZZLE BnaC07g40830D [Brassica napus]
BnaA07G0282700ZS Down — K16223 Protein TWIN SISTER of FT OS=Arabidopsis thaliana GN=TSF PE=2 SV=1 Phosphatidylethanolamine-binding protein PREDICTED: protein TWIN SISTER of FT-like [Brassica rapa]
BnaC06G0323800ZS Down — K16223 Protein FLOWERING LOCUS T OS=Arabidopsis thaliana GN=FT PE=1 SV=2 Phosphatidylethanolamine-binding protein flowering locus T variant 6 [Brassica carinata]
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