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作物学报

作物学报 ›› 2025, Vol. 51 ›› Issue (1): 117-133.doi: 10.3724/SP.J.1006.2025.44077

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

绿豆R2R3-MYB转录因子家族鉴定及其类黄酮合成调控基因的筛选

郭飞翔(), 李春霞(), 周爽, 郭彬彬, 张均, 马超()   

  1. 河南科技大学农学院 / 河南省旱地农业工程技术研究中心, 河南洛阳 471000
  • 收稿日期:2024-05-10 接受日期:2024-08-15 出版日期:2025-01-12 网络出版日期:2024-08-27
  • 通讯作者: 马超
  • 作者简介:郭飞翔, E-mail: gfx0405@163.com;
    李春霞, E-mail: lichx@haust.edu.cn第一联系人：**同等贡献
  • 基金资助:
    国家自然科学基金项目(32372227);河南省高等学校青年骨干教师培养计划项目(2021GGJS050);河南省现代农业产业技术体系建设专项(HARS-22-04-G2)

Identification of the R2R3-MYB transcription factor family and screening of genes regulating flavonoid synthesis in mung bean

GUO Fei-Xiang(), LI Chun-Xia(), ZHOU Shuang, GUO Bin-Bin, ZHANG Jun, MA Chao()   

  1. Agronomy College, Henan University of Science and Technology / Dry-land Agricultural Engineering Technology Research Center in Henan, Luoyang 471000, Henan, China
  • Received:2024-05-10 Accepted:2024-08-15 Published:2025-01-12 Published online:2024-08-27
  • Contact: MA Chao
  • About author:First author contact:**Contributed equally to this work
  • Supported by:
    National Natural Science Foundation of China(32372227);Training Program for University Young Key Teachers in Henan Province(2021GGJS050);Special Fund for Henan Agriculture Research System(HARS-22-04-G2)

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摘要：

R2R3-MYB转录因子家族在植物次生代谢物合成、胁迫应答和生长发育等生命过程起着重要的调控作用。本研究基于生物信息学鉴定了绿豆(Vigna radiata L.)全基因组水平的R2R3-MYB转录因子, 并对其理化性质、系统进化、染色体定位、启动子顺式作用元件及基因结构做了预测分析; 此外, 转录组数据和实时荧光定量PCR (Quantitative Real-time PCR, RT-PCR)分析该转录因子在不同组织、逆境胁迫下的表达模式; 并基于相关分析及蛋白互作网络, 筛选到可能参与调控绿豆类黄酮生物合成的R2R3-MYB成员。结果表明, 共鉴定到168个R2R3-MYB成员, 其中145个分布于11条染色体, 23个成员染色体信息未知; 大多数R2R3-MYB含有3个外显子, 编码99~1645个氨基酸, 均为亲水性蛋白; 系统进化将绿豆R2R3-MYB基因家族分为30个亚组(V1~V30), 不同亚组成员的基因结构存在差异; 共线性分析表明, 片段复制事件均进行了纯化选择; 启动子顺式作用元件分析表明, 绿豆R2R3-MYB基因启动子区含有大量激素响应、胁迫响应及少量的类黄酮合成响应等元件; 基因表达分析表明, 在叶片、叶柄、下胚轴和籽粒种皮中表达量较高的成员分别占15.5%、16.1%、16.1%和10.7%。RT-PCR分析发现, 几乎所有的R2R3-MYB家族成员在低温胁迫下的相对表达量显著下调, 不同成员对逆境胁迫有不同的响应模式。蛋白互作与相关性分析可知, VrMYB6、VrMYB77、VrMYB93这3个基因可能参与了绿豆类黄酮生物合成的调控。

关键词: 绿豆, R2R3-MYB转录因子, 转录因子家族分析, 类黄酮合成调控基因

Abstract:

The R2R3-MYB transcription factor family plays a crucial regulatory role in the synthesis of secondary metabolites, stress responses, and the growth and development of plants. This study employed bioinformatics analysis to identify the R2R3-MYB transcription factors across the entire genome of mung bean (Vigna radiata L.), and to predict their physicochemical properties, phylogenetic evolution, chromosome localization, cis-acting elements of the promoter, and gene structure. The expression patterns of mung bean under different tissues, exogenous hormones, and stress conditions were analyzed using transcriptome data and Quantitative Real-time PCR (RT-PCR). R2R3-MYB members potentially involved in regulating the biosynthesis of mung bean flavonoids were screened through correlation analysis and protein interaction networks. The results demonstrated that a total of 168 R2R3-MYB members were identified in mung bean, 145 of which were distributed across 11 chromosomes, while the chromosome information for 23 members remains unknown. Most of these members contain three exons encoding proteins ranging from 99 to 1645 amino acids and are all hydrophilic. The phylogenetic analysis divided the R2R3-MYB gene family in mung beans into 30 subgroups (V1-V30), revealing structural differences among members of different subgroups. Collinearity analysis within the mung bean genome indicated that all segmental duplication events underwent purifying selection. Analysis of cis-acting elements in the promoter regions of the R2R3-MYB genes in mung beans revealed a large number of hormone-responsive and stress-responsive elements, as well as a small number of flavonoid synthesis-responsive elements. Gene expression analysis showed that members with higher expression levels in leaves, petioles, hypocotyls, and seed coats accounted for 15.5%, 16.1%, 16.1%, and 10.7% of the total, respectively. RT-PCR analysis indicated that the relative expression levels of almost all R2R3-MYB family members significantly decreased under low-temperature stress, with different members had diverse response patterns to stress. Protein interaction and correlation analysis suggested that the genes VrMYB6, VrMYB77, and VrMYB93 may be involved in the regulation of flavonoid biosynthesis in mung beans. The results of this study lay the foundation for further in-depth research on the function of the R2R3-MYB transcription factor family in mung beans.

Key words: mung bean, R2R3-MYB transcription factor, transcription factor family analysis, flavonoids synthesis regulatory genes

表1

本试验所用引物"

登录号
Accession number		 基因名称
Gene name		 上游引物
Forward primer (5'-3')		 下游引物
Reverse primer (5'-3')
XP_014495171.1 VrMYB39 AAACTTCAGGCTGTTCTTGGC AGATTGGTGTGGAGTGGCATT
XP_014495344.2 VrMYB40 ATGGACCAACTATCTCCGACCT TTCCTCACTCTGCATCTTCTTCA
XP_014497212.1 VrMYB50 GAAGAGTTGTAGACTGAGATGGTTG CTGTTGTGAGATTGTACTTTGCG
XP_014500543.1 VrMYB52 GATGGTCAATGATTGCAGGAA CGAGTCTTGTCACGAGAGGCT
XP_014505357.1 VrMYB68 GGAAGAGAGCCAAAGCACAGA AGAAGAGGCAAGATAACCAGGAA
XP_014505359.1 VrMYB72 GCCACTTCACCCTTCACCTTA TTCTCCAATCACCTTTCCCATA
XP_014508007.1 VrMYB79 TCTTTACCTTATCCCACCTCG AGCAATTAATGGCTTCCACTG
XP_014505860.1 VrMYB84 ACGACGGTCCCACCACCT ATTCAACGCTCCCATTCCAA
XP_014510156.1 VrMYB96 CTACTTCAAAGAGCGTCAGCAGT GGTCAGAAATGTATTGGGAGAGC
XP_022638546.1 VrMYB98 AGTGACTGTGGGCTTGGACAC TAGGACATTGCTGCTGCGTT
XP_014513300.1 VrMYB111 ACGGCAGGAGCAGTAGGGA CCAAGAGTTCACGAAATCGGA
XP_022631357.1 VrMYB133 GTAACAACAAAAGCAGCGGTG CAATGGTGGCAGATAATTCGTA
XP_014521508.1 VrMYB137 TACTTCCCTTTCCCTGTCCC AACTCCTCACCTCCTTCCTTATC
XP_014491137.1 VrMYB156 CTGCTGCGGAATCTTCTCAC ACTCCATCATCTCCTCCACTTG
NP_001304238.1 Actin GTTCTGTTCCAGCCATCCAT GTGGTGCGACAACCTTGATT

图1

绿豆(☆)与拟南芥(★) R2R3-MYB基因家族成员的系统进化树"

附表1

绿豆R2R3-MYB蛋白理化性质和亚细胞定位"

基因名称Gene name 登录号
Accession number		 氨基酸数目
No. of amino acids		 相对分子质量Molecular weight (Da) 等电点
pI		 不稳定指数Instability index 脂肪系数
Aliphatic index		 平均疏水指数
Grand average of hydropathicity		 亚细胞定位
Subcellular localization
VrMYB2 XP_014490478.1 353 40,519.12 9.42 55.78 62.95 -0.804 细胞核Nuclear
VrMYB154 XP_014490560.1 447 50,974.45 6.72 49.59 66.76 -0.748 细胞核Nuclear
VrMYB155 XP_014490587.1 369 41,290.28 5.72 57.00 67.13 -0.538 细胞核Nuclear
VrMYB29 XP_014491051.2 213 24,945.49 8.91 45.59 69.53 -0.759 细胞核Nuclear
VrMYB156 XP_014491137.1 336 37,977.18 6.28 61.76 62.74 -0.903 细胞核Nuclear
VrMYB157 XP_014491218.1 353 39,488.24 7.66 56.63 64.33 -0.728 细胞核Nuclear
VrMYB158 XP_014491519.1 279 31,560.73 9.00 49.20 71.94 -0.845 细胞核Nuclear
VrMYB159 XP_014491522.1 222 25,046.15 8.77 53.49 67.25 -0.728 细胞核Nuclear
VrMYB17 XP_014491580.1 254 28,186.93 8.97 49.55 76.89 -0.573 细胞核Nuclear
VrMYB160 XP_014491673.1 295 34,124.76 5.06 45.76 68.71 -0.740 细胞核Nuclear
VrMYB161 XP_014491740.1 326 36,571.14 6.17 44.82 82.85 -0.532 细胞核Nuclear
VrMYB162 XP_014492023.1 354 40,197.79 9.31 58.95 72.54 -0.797 细胞核Nuclear
VrMYB163 XP_014492260.1 321 35,431.69 8.65 48.69 75.70 -0.597 细胞核Nuclear
VrMYB164 XP_014492289.1 303 34,033.61 8.06 43.08 76.27 -0.519 细胞核Nuclear
VrMYB165 XP_014492385.1 446 49,816.51 7.19 58.09 59.30 -0.731 细胞核Nuclear
VrMYB166 XP_014492596.1 474 53,985.72 5.67 60.76 75.53 -0.753 细胞核Nuclear
VrMYB167 XP_014492680.1 223 25,598.86 5.71 63.01 73.00 -0.770 细胞核Nuclear
VrMYB24 XP_014493503.1 299 33,897.71 5.28 54.91 64.48 -0.592 细胞核Nuclear
VrMYB20 XP_014493509.1 308 34,486.58 6.32 58.14 61.10 -0.695 细胞核Nuclear
VrMYB30 XP_014493577.1 290 33,354.49 6.06 51.73 66.90 -0.671 细胞核Nuclear
VrMYB25 XP_014493625.1 312 34,732.4 4.95 56.11 60.64 -0.574 细胞核Nuclear
VrMYB27 XP_014493695.1 235 27,330.3 5.71 54.02 54.38 -0.913 细胞核Nuclear
VrMYB21 XP_014493915.2 969 109,225.52 5.34 51.49 70.24 -0.905 细胞核Nuclear
VrMYB18 XP_014494074.1 327 36,606.69 5.52 57.90 65.29 -0.652 细胞核Nuclear
VrMYB7 XP_014494133.1 273 30,238.8 5.75 50.18 72.56 -0.483 细胞核Nuclear
VrMYB8 XP_014494335.1 307 35,125.92 5.25 66.86 63.91 -0.764 细胞核Nuclear
VrMYB36 XP_014494877.1 404 45,089.38 6.64 58.14 67.65 -0.688 细胞核Nuclear
VrMYB35 XP_014495060.1 365 41,348.45 6.28 66.58 73.95 -0.571 细胞核Nuclear
VrMYB39 XP_014495171.1 393 43,217.33 8.15 51.87 69.72 -0.610 细胞核Nuclear
VrMYB37 XP_014495198.1 439 48,752.97 6.96 50.96 59.54 -0.752 细胞核Nuclear
VrMYB38 XP_014495343.1 296 34,406.21 8.88 58.65 67.84 -0.690 细胞核Nuclear
VrMYB40 XP_014495344.2 356 40,586.71 8.50 59.69 61.66 -0.827 细胞核Nuclear
VrMYB32 XP_014495439.1 334 37,009.28 6.89 47.45 61.35 -0.583 细胞核Nuclear
VrMYB33 XP_014495618.1 353 40,571.45 5.99 51.36 63.51 -0.874 细胞核Nuclear
VrMYB34 XP_014495632.1 276 30,743.97 5.78 50.45 63.66 -0.615 细胞核Nuclear
VrMYB31 XP_014495663.1 288 32,668.16 5.01 59.29 67.08 -0.835 细胞核Nuclear
VrMYB49 XP_014496414.1 393 43,324.87 6.37 52.14 60.84 -0.782 细胞核Nuclear
VrMYB51 XP_014496890.2 266 30,532.81 7.57 46.40 62.63 -0.666 细胞核Nuclear
VrMYB48 XP_014497102.1 317 35,260.48 8.50 57.97 60.38 -0.735 细胞核Nuclear
VrMYB50 XP_014497212.1 233 26,831.1 7.65 42.12 68.97 -0.804 细胞核Nuclear
VrMYB43 XP_014497533.1 332 37,589.87 6.98 61.47 59.61 -0.757 细胞核Nuclear
VrMYB47 XP_014497557.1 308 34,506.71 8.13 50.69 68.15 -0.675 细胞核Nuclear
VrMYB45 XP_014497590.1 202 23,404.17 6.52 54.95 61.83 -0.972 细胞核Nuclear
VrMYB44 XP_014497598.1 199 23,201.05 7.71 54.97 60.80 -0.897 细胞核Nuclear
VrMYB42 XP_014497919.1 245 28,010.17 5.85 51.28 51.31 -0.923 细胞核Nuclear
VrMYB63 XP_014498875.1 510 56,364.71 4.83 46.30 62.55 -0.688 细胞核Nuclear
VrMYB55 XP_014499283.1 332 37,079.62 6.07 54.79 72.62 -0.584 细胞核Nuclear
VrMYB64 XP_014499553.1 465 51,891.28 5.94 41.56 53.76 -0.956 细胞核Nuclear
VrMYB65 XP_014499570.1 241 27,677.98 6.23 57.75 71.62 -0.715 细胞核Nuclear
VrMYB59 XP_014499816.1 258 28,897.52 8.02 56.23 74.22 -0.753 细胞核Nuclear
VrMYB54 XP_014500310.1 341 38,408 5.84 54.17 72.55 -0.502 细胞核Nuclear
VrMYB61 XP_014500370.1 356 40,431.79 5.04 58.23 58.37 -0.887 细胞核Nuclear
VrMYB57 XP_014500397.1 255 28,177.83 9.58 49.35 64.67 -0.788 细胞核Nuclear
VrMYB52 XP_014500543.1 168 19,498.29 8.86 54.24 73.63 -0.783 细胞核Nuclear
VrMYB58 XP_014500580.2 316 35,386.47 8.61 51.44 73.16 -0.592 细胞核Nuclear
VrMYB56 XP_014500680.1 341 37,894.6 7.49 56.44 74.05 -0.581 细胞核Nuclear
VrMYB62 XP_014500689.1 354 39,760.86 6.11 52.36 85.65 -0.392 细胞核Nuclear
VrMYB60 XP_014500881.2 335 38,140.87 6.56 58.61 75.94 -0.566 细胞核Nuclear
VrMYB67 XP_014502988.1 229 26,557.09 9.76 60.71 70.70 -0.881 细胞核Nuclear
VrMYB71 XP_014503089.1 322 37,763.5 9.03 57.96 61.74 -0.966 细胞核Nuclear
VrMYB74 XP_014503316.1 239 26,950.93 7.90 50.64 58.49 -0.765 细胞核Nuclear
VrMYB77 XP_014503606.1 274 30,061.36 9.53 64.13 73.39 -0.546 细胞核Nuclear
VrMYB70 XP_014503716.1 322 36,800.06 4.90 52.65 69.97 -0.794 细胞核Nuclear
VrMYB76 XP_014504095.1 368 41,266.76 6.41 55.04 62.26 -0.761 细胞核Nuclear
VrMYB75 XP_014504137.1 311 34,682.76 8.10 57.24 63.38 -0.706 细胞核Nuclear
VrMYB69 XP_014504153.2 300 34,017.88 9.40 59.97 85.57 -0.54 细胞核Nuclear
VrMYB73 XP_014504193.2 415 46,264.58 6.50 51.14 73.28 -0.622 细胞核Nuclear
VrMYB78 XP_014504223.1 195 21,915.55 10.44 44.37 63.54 -0.756 细胞核Nuclear
VrMYB68 XP_014505357.1 317 36,325.3 8.12 51.91 66.72 -0.572 细胞核Nuclear
VrMYB72 XP_014505359.1 304 34,265.21 8.73 56.32 57.7 -0.796 细胞核Nuclear
VrMYB84 XP_014505860.1 288 31,053.32 9.06 60.05 72.19 -0.322 细胞核Nuclear
VrMYB5 XP_014506098.1 319 35,110.35 8.42 51.17 68.56 -0.602 细胞核Nuclear
VrMYB82 XP_014506348.1 399 44,463.32 5.96 54.87 71.15 -0.614 细胞核Nuclear
VrMYB10 XP_014506438.1 356 40,139.75 6.08 51.58 60.81 -0.735 细胞核Nuclear
VrMYB93 XP_014506712.1 245 27,984.64 8.13 51.43 75.63 -0.718 细胞核Nuclear
VrMYB95 XP_014506748.1 164 19,056.48 9.62 51.35 82.07 -0.598 细胞核Nuclear
VrMYB97 XP_014506838.2 298 33,320.62 7.06 44.96 78.79 -0.399 细胞核Nuclear
VrMYB81 XP_014506887.1 450 49,710.71 6.06 57.01 54.89 -0.729 细胞核Nuclear
VrMYB91 XP_014507110.1 295 32,234.85 6.98 65.33 59.86 -0.625 细胞核Nuclear
VrMYB89 XP_014507552.1 363 41,251.18 5.94 53.28 69.56 -0.658 细胞核Nuclear
VrMYB13 XP_014507919.1 555 60,998.53 5.57 45.88 64.88 -0.653 细胞核Nuclear
VrMYB94 XP_014507961.1 306 35,249.24 6.10 53.99 71.70 -0.769 细胞核Nuclear
VrMYB79 XP_014508007.1 225 25,636.07 9.08 55.83 80.22 -0.764 细胞核Nuclear
VrMYB90 XP_014508126.1 312 35,048.85 6.16 50.92 58.85 -0.774 细胞核Nuclear
VrMYB9 XP_014508294.1 229 26,995.16 8.51 60.83 60.00 -0.913 细胞核Nuclear
VrMYB92 XP_014508343.1 361 40,704.39 6.16 65.30 69.47 -0.751 细胞核Nuclear
VrMYB86 XP_014508468.1 198 22,943.64 6.60 57.67 74.39 -0.908 细胞核Nuclear
VrMYB80 XP_014508588.1 488 55,604.67 5.91 58.75 63.95 -0.988 细胞核Nuclear
VrMYB11 XP_014509201.2 358 39,544.61 8.89 56.66 67.54 -0.563 细胞核Nuclear
VrMYB87 XP_014509393.1 271 30,693.45 5.15 50.54 78.12 -0.68 细胞核Nuclear
VrMYB4 XP_014509585.2 399 44,662.72 7.64 51.72 79.65 -0.357 细胞核Nuclear
VrMYB15 XP_014509801.1 289 32,833.83 7.22 50.58 67.82 -0.757 细胞核Nuclear
VrMYB3 XP_014509861.2 251 29,115.64 7.87 63.01 73.47 -0.795 细胞核Nuclear
VrMYB96 XP_014510156.1 314 36,063.06 6.21 58.06 62.74 -0.836 细胞核Nuclear
VrMYB107 XP_014510965.1 262 30,135.86 5.67 65.63 60.34 -0.730 细胞核Nuclear
VrMYB100 XP_014511116.1 258 29,566.02 5.62 62.92 59.34 -0.777 细胞核Nuclear
VrMYB110 XP_014511901.1 273 31,804.92 6.21 44.41 63.22 -0.815 细胞核Nuclear
VrMYB112 XP_014511935.1 318 35,764.29 6.96 53.02 74.47 -0.584 细胞核Nuclear
VrMYB106 XP_014512306.1 395 44,483.57 6.60 54.21 65.42 -0.727 细胞核Nuclear
VrMYB104 XP_014512332.1 257 29,779.93 9.01 55.67 81.56 -0.589 细胞核Nuclear
VrMYB115 XP_014512566.1 241 27,351.48 6.14 51.98 61.83 -0.764 细胞核Nuclear
VrMYB102 XP_014512645.1 316 34,687.82 7.09 53.99 60.44 -0.615 细胞核Nuclear
VrMYB14 XP_014512651.1 969 109,188.43 5.36 51.82 70.72 -0.901 细胞核Nuclear
VrMYB114 XP_014512719.1 305 34,691 6.40 66.70 63.67 -0.730 细胞核Nuclear
VrMYB103 XP_014512761.1 360 40,628.34 5.20 55.12 76.36 -0.696 细胞核Nuclear
VrMYB101 XP_014513141.2 287 33,282.66 9.34 63.54 65.3 -0.805 细胞核Nuclear
VrMYB109 XP_014513204.1 261 30,035.61 5.10 53.88 70.27 -0.734 细胞核Nuclear
VrMYB105 XP_014513208.1 342 39,172.99 7.07 49.85 69.85 -0.863 细胞核Nuclear
VrMYB113 XP_014513226.1 269 30,264.31 9.01 64.58 70.04 -0.662 细胞核Nuclear
VrMYB108 XP_014513230.1 267 31,116.31 9.11 61.24 69.4 -0.842 细胞核Nuclear
VrMYB111 XP_014513300.1 1645 179,255.55 5.82 45.51 68.21 -0.614 细胞核Nuclear
VrMYB124 XP_014515157.1 352 38,079.81 6.23 46.47 73.75 -0.499 细胞核Nuclear
VrMYB116 XP_014515250.1 326 37,097.32 6.19 58.75 78.10 -0.453 细胞核Nuclear
VrMYB117 XP_014515266.1 266 30,146.01 8.45 60.91 74.77 -0.852 细胞核Nuclear
VrMYB118 XP_014515267.1 292 33,211.04 6.46 54.81 65.75 -0.819 细胞核Nuclear
VrMYB121 XP_014515433.2 320 36,512.18 5.86 65.81 68.84 -0.679 细胞核Nuclear
VrMYB125 XP_014515544.1 355 40,712.28 9.36 60.40 60.14 -0.721 细胞核Nuclear
VrMYB123 XP_014515811.1 350 40,036.64 8.96 64.20 75.63 -0.787 细胞核Nuclear
VrMYB120 XP_014516586.1 279 31,167.14 8.08 56.03 72.08 -0.623 细胞核Nuclear
VrMYB122 XP_014516816.1 359 41,072.71 9.12 56.34 75.68 -0.784 细胞核Nuclear
VrMYB126 XP_014517284.1 325 36,384.97 6.63 41.73 65.48 -0.631 细胞核Nuclear
VrMYB127 XP_014517297.1 412 46,439.76 9.05 51.83 70.29 -0.771 细胞核Nuclear
VrMYB129 XP_014517902.1 374 42,214.16 5.94 53.07 71.66 -0.627 细胞核Nuclear
VrMYB6 XP_014518062.1 408 44,437.02 5.93 56.82 63.41 -0.738 细胞核Nuclear
VrMYB130 XP_014518874.1 637 72,923.96 8.81 53.79 64.69 -1.002 细胞核Nuclear
VrMYB128 XP_014518898.1 320 36,181.58 9.07 59.95 60.34 -0.763 过氧化物酶体Peroxisomal
VrMYB19 XP_014519116.1 259 30,015.36 5.47 66.20 59.85 -0.822 细胞核Nuclear
VrMYB135 XP_014519579.1 179 20,859.68 9.53 56.90 71.40 -0.761 细胞核Nuclear
VrMYB139 XP_014519753.2 454 50,306.7 7.58 53.06 53.72 -0.701 细胞核Nuclear
VrMYB141 XP_014519828.1 284 31,907.4 8.87 46.97 84.15 -0.471 细胞核Nuclear
VrMYB142 XP_014519830.1 301 33,798.42 8.72 51.25 80.7 -0.509 细胞核Nuclear
VrMYB143 XP_014519831.1 284 31,877.33 8.87 46.12 82.78 -0.490 细胞核Nuclear
VrMYB144 XP_014519832.2 316 36,076.88 8.91 46.6 75.28 -0.684 细胞核Nuclear
VrMYB145 XP_014519833.1 301 33,771.47 7.68 44.26 79.73 -0.516 细胞核Nuclear
VrMYB134 XP_014520093.1 265 30,135.08 5.45 47.93 79.51 -0.649 细胞核Nuclear
VrMYB136 XP_014520380.1 350 39,831.52 6.75 49.12 65.94 -0.725 细胞核Nuclear
VrMYB138 XP_014520455.2 344 38,854.71 6.80 47.11 73.72 -0.697 细胞核Nuclear
VrMYB132 XP_014520504.1 303 34,206.42 6.59 44.17 77.59 -0.628 细胞核Nuclear
VrMYB140 XP_014520829.1 293 31,226.46 6.37 49.98 65.26 -0.600 细胞核Nuclear
VrMYB137 XP_014521508.1 310 33,733.35 9.27 54.04 73.55 -0.454 细胞核Nuclear
VrMYB146 XP_014521761.1 274 30,927.32 7.72 57.48 65.88 -0.836 细胞核Nuclear
VrMYB147 XP_014522324.1 336 38,530.62 8.97 60.39 55.74 -0.913 细胞核Nuclear
VrMYB16 XP_014523159.1 341 38,315.16 5.69 51.97 78.09 -0.543 细胞核Nuclear
VrMYB148 XP_014523541.1 286 32,163.29 5.92 53.84 82.83 -0.486 细胞核Nuclear
VrMYB149 XP_014523608.1 294 31,231.63 6.80 48.98 63.44 -0.645 细胞核Nuclear
VrMYB26 XP_014523889.1 224 25,899.25 6.72 43.28 70.62 -0.696 细胞核Nuclear
VrMYB28 XP_014523933.1 308 34,382.87 6.00 51.64 81.40 -0.483 细胞核Nuclear
VrMYB151 XP_014524064.1 374 42,360.42 8.81 53.39 74.30 -0.697 细胞核Nuclear
VrMYB152 XP_014524093.1 289 32,729.65 7.63 51.61 60.10 -0.979 细胞核Nuclear
VrMYB153 XP_014524479.1 526 58,840.02 5.33 49.40 65.48 -0.785 细胞核Nuclear
VrMYB133 XP_022631357.1 510 57,347.4 8.18 55.01 62.53 -0.676 细胞核Nuclear
VrMYB131 XP_022631644.1 397 45,022.38 6.94 42.96 88.14 -0.276 细胞核Nuclear
VrMYB150 XP_022632584.1 514 56,968.81 5.79 56.18 58.11 -0.746 细胞核Nuclear
VrMYB168 XP_022633932.1 386 43,709.37 6.28 57.89 67.98 -0.710 细胞核Nuclear
VrMYB12 XP_022634264.1 378 41,994.41 8.73 54.57 74.05 -0.513 细胞核Nuclear
VrMYB22 XP_022634320.1 99 11,526.34 10.4 66.57 66.06 -0.810 细胞核Nuclear
VrMYB23 XP_022634322.1 219 25,489.04 5.56 48.64 59.77 -0.859 细胞核Nuclear
VrMYB41 XP_022635231.1 312 35,451.36 5.52 58.31 59.04 -0.720 细胞核Nuclear
VrMYB46 XP_022635873.1 225 26,354.19 9.61 68.18 64.98 -0.902 细胞核Nuclear
VrMYB53 XP_022635910.1 415 47,715.06 6.29 54.57 69.54 -0.708 细胞核Nuclear
VrMYB66 XP_022637422.1 237 27,126.58 9.59 54.49 63.33 -0.965 细胞核Nuclear
VrMYB88 XP_022638520.1 189 21,854.73 8.97 49.45 63.39 -0.801 细胞核Nuclear
VrMYB98 XP_022638546.1 525 58,703.1 7.22 53.37 66.48 -0.613 细胞核Nuclear
VrMYB99 XP_022638786.1 282 31,347.02 8.47 52.64 66.77 -0.819 细胞核Nuclear
VrMYB83 XP_022640184.1 241 27,649.18 8.11 52.17 66.76 -0.822 细胞核Nuclear
VrMYB85 XP_022640223.1 304 34,030.11 7.62 55.37 69.31 -0.584 细胞核Nuclear
VrMYB1 XP_022640949.1 266 30,826.14 9.73 53.47 60.15 -0.840 细胞核Nuclear
VrMYB119 XP_022641765.1 294 32,984.46 8.52 55.40 77.69 -0.540 细胞核Nuclear

附表2

绿豆R2R3-MYB蛋白的二级结构预测"

登录号
Accession number		 α-螺旋
Alpha helix (Hh)		 β-转角
Beta turn (Tt)		 延伸链
Extended strand (Ee)		 无规则卷曲
Random coil (Cc)
XP_014490478.1 28.05 4.82 10.76 56.37
XP_014490560.1 28.41 2.91 6.04 62.64
XP_014490587.1 32.25 3.79 7.05 56.91
XP_014491051.2 43.19 6.10 1.88 48.83
XP_014491137.1 34.52 2.08 5.65 57.74
XP_014491218.1 27.76 5.67 10.20 56.37
XP_014491519.1 36.20 4.66 14.70 44.44
XP_014491522.1 29.73 6.76 6.76 56.76
XP_014491580.1 33.46 5.12 10.24 51.18
XP_014491673.1 37.29 5.76 5.08 51.86
XP_014491740.1 30.98 2.45 7.67 58.90
XP_014492023.1 53.67 2.26 3.95 40.11
XP_014492260.1 18.69 4.67 14.33 62.31
XP_014492289.1 38.28 5.28 8.25 48.18
XP_014492385.1 28.70 3.36 3.59 64.35
XP_014492596.1 36.92 4.01 7.59 51.48
XP_014492680.1 41.70 5.38 4.93 47.98
XP_014493503.1 30.43 4.35 8.70 56.52
XP_014493509.1 23.05 5.19 13.31 58.44
XP_014493577.1 31.72 5.52 6.55 56.21
XP_014493625.1 35.90 5.45 5.45 53.21
XP_014493695.1 40.85 6.81 6.81 45.53
XP_014493915.2 53.66 1.75 4.44 40.14
XP_014494074.1 28.75 5.20 8.87 57.19
XP_014494133.1 31.87 7.69 14.65 45.79
XP_014494335.1 32.25 4.56 8.47 54.72
XP_014494877.1 32.18 3.96 4.70 59.16
XP_014495060.1 34.25 8.22 14.52 43.01
XP_014495171.1 35.11 7.12 11.70 46.06
XP_014495198.1 25.97 4.10 4.56 65.38
XP_014495343.1 28.72 4.73 17.57 48.99
XP_014495344.2 29.21 2.53 8.15 60.11
XP_014495439.1 27.54 7.78 16.77 47.90
XP_014495618.1 35.13 5.95 15.01 43.91
XP_014495632.1 40.58 5.07 9.42 44.93
XP_014495663.1 35.07 3.47 5.21 56.25
XP_014496414.1 33.59 5.09 10.43 50.89
XP_014496890.2 37.59 6.02 7.89 48.50
XP_014497102.1 36.59 0.63 4.42 58.36
XP_014497212.1 35.62 9.44 9.44 45.49
XP_014497533.1 23.19 4.82 13.86 58.13
XP_014497557.1 36.04 5.84 6.82 51.30
XP_014497590.1 33.66 5.45 5.45 55.45
XP_014497598.1 33.17 5.03 6.03 55.78
XP_014497919.1 38.37 6.12 6.94 48.57
XP_014498875.1 26.27 4.12 6.47 63.14
XP_014499283.1 35.84 3.31 1.20 59.64
XP_014499553.1 26.45 4.09 4.73 64.73
XP_014499570.1 32.78 5.39 7.05 54.77
XP_014499816.1 34.11 6.20 6.98 52.71
XP_014500310.1 33.43 3.81 5.57 57.18
XP_014500370.1 27.25 4.78 8.15 59.83
XP_014500397.1 38.43 5.10 7.84 48.63
XP_014500543.1 38.10 9.52 1.79 50.60
XP_014500580.2 31.33 6.96 19.94 41.77
XP_014500680.1 31.09 2.93 6.16 59.82
XP_014500689.1 29.94 4.52 6.50 59.04
XP_014500881.2 42.09 5.07 8.36 44.48
XP_014502988.1 44.10 6.99 7.86 41.05
XP_014503089.1 27.02 4.35 15.22 53.42
XP_014503316.1 31.80 5.44 7.95 54.81
XP_014503606.1 33.58 3.65 5.84 56.93
XP_014503716.1 33.54 4.35 6.21 55.90
XP_014504095.1 26.09 4.62 13.59 55.71
XP_014504137.1 27.65 5.79 7.07 59.49
XP_014504153.2 28.33 5.00 10.33 56.33
XP_014504193.2 22.65 6.02 14.22 57.11
XP_014504223.1 40.51 7.69 7.18 44.62
XP_014505357.1 30.91 4.10 13.88 51.10
XP_014505359.1 20.39 3.29 17.76 58.55
XP_014505860.1 29.51 3.82 5.56 61.11
XP_014506098.1 33.54 2.51 4.08 59.87
XP_014506348.1 34.59 3.51 8.02 53.88
XP_014506438.1 26.97 4.21 8.99 59.83
XP_014506712.1 34.29 6.12 6.94 52.65
XP_014506748.1 50.00 6.71 6.71 36.59
XP_014506838.2 24.50 11.07 18.12 46.31
XP_014506887.1 24.44 3.78 10.22 61.56
XP_014507110.1 20.00 2.03 12.20 65.76
XP_014507552.1 33.61 3.31 6.89 56.20
XP_014507919.1 25.41 3.60 4.32 66.67
XP_014507961.1 37.58 2.61 6.21 53.59
XP_014508007.1 25.33 6.22 5.78 62.67
XP_014508126.1 34.62 2.88 4.49 58.01
XP_014508294.1 31.88 4.37 8.73 55.02
XP_014508343.1 36.01 2.22 4.71 57.06
XP_014508468.1 44.95 6.57 3.54 44.95
XP_014508588.1 36.68 3.28 8.61 51.43
XP_014509201.2 23.18 5.03 11.73 60.06
XP_014509393.1 36.53 4.06 5.90 53.51
XP_014509585.2 33.83 4.76 6.52 54.89
XP_014509801.1 32.53 5.54 4.50 57.44
XP_014509861.2 36.25 5.18 4.78 53.78
XP_014510156.1 32.17 3.82 4.78 59.24
XP_014510965.1 22.14 4.96 4.58 68.32
XP_014511116.1 30.62 4.26 3.10 62.02
XP_014511901.1 43.22 4.03 5.13 47.62
XP_014511935.1 34.91 6.29 12.89 45.91
XP_014512306.1 26.84 4.81 3.04 65.32
XP_014512332.1 39.30 6.61 9.34 44.75
XP_014512566.1 23.24 3.32 14.11 59.34
XP_014512645.1 25.00 6.33 12.66 56.01
XP_014512651.1 53.66 2.68 4.54 39.11
XP_014512719.1 31.48 3.93 6.56 58.03
XP_014512761.1 35.83 5.00 13.06 46.11
XP_014513141.2 47.04 8.36 9.06 35.54
XP_014513204.1 38.31 4.98 5.75 50.96
XP_014513208.1 39.77 5.26 3.80 51.17
XP_014513226.1 37.92 5.58 5.58 50.93
XP_014513230.1 48.69 5.62 8.99 36.70
XP_014513300.1 26.32 2.37 9.42 61.88
XP_014515157.1 25.28 3.98 11.08 59.66
XP_014515250.1 26.99 4.60 3.99 64.42
XP_014515266.1 27.44 6.39 3.38 62.78
XP_014515267.1 29.11 6.85 7.88 56.16
XP_014515433.2 23.75 3.75 9.38 63.12
XP_014515544.1 29.58 5.35 15.49 49.58
XP_014515811.1 56.00 4.00 4.00 36.00
XP_014516586.1 34.41 4.30 5.73 55.56
XP_014516816.1 56.55 4.18 5.29 33.98
XP_014517284.1 35.06 5.54 5.54 53.85
XP_014517297.1 30.34 4.61 9.95 55.10
XP_014517902.1 27.54 4.28 5.61 62.57
XP_014518062.1 29.41 3.92 9.31 57.35
XP_014518874.1 59.34 3.14 5.81 31.71
XP_014518898.1 22.81 2.81 14.06 60.31
XP_014519116.1 28.19 8.49 11.58 51.47
XP_014519579.1 41.90 7.82 7.26 43.02
XP_014519753.2 25.55 3.74 11.89 58.81
XP_014519828.1 38.38 4.93 7.75 48.94
XP_014519830.1 38.54 4.98 13.29 43.19
XP_014519831.1 41.20 5.99 8.10 44.72
XP_014519832.2 36.71 5.70 9.18 48.42
XP_014519833.1 36.88 3.99 11.63 47.51
XP_014520093.1 34.72 4.53 5.66 55.09
XP_014520380.1 36.86 4.57 9.14 49.43
XP_014520455.2 39.53 4.94 4.65 50.87
XP_014520504.1 43.56 3.30 5.94 47.19
XP_014520829.1 18.09 4.44 12.63 64.85
XP_014521508.1 25.48 3.87 6.77 63.87
XP_014521761.1 24.82 4.01 4.01 67.15
XP_014522324.1 25.60 2.38 10.42 61.61
XP_014523159.1 41.06 3.81 3.52 51.61
XP_014523541.1 41.26 5.59 9.79 43.36
XP_014523608.1 19.05 3.74 12.24 64.97
XP_014523889.1 31.70 7.14 6.70 54.46
XP_014523933.1 35.71 3.25 5.52 55.52
XP_014524064.1 35.83 5.08 8.29 50.80
XP_014524093.1 26.64 4.84 12.11 56.40
XP_014524479.1 22.43 3.04 5.89 68.63
XP_022631357.1 27.45 4.90 6.27 61.37
XP_022631644.1 41.31 4.53 13.60 40.55
XP_022632584.1 19.07 5.06 10.51 65.37
XP_022633932.1 32.90 4.40 6.99 55.70
XP_022634264.1 25.93 6.61 12.43 55.03
XP_022634320.1 33.33 9.09 8.08 49.49
XP_022634322.1 36.07 5.94 4.11 53.88
XP_022635231.1 36.22 4.49 8.01 51.28
XP_022635873.1 54.22 4.89 3.11 37.78
XP_022635910.1 30.84 3.61 8.67 56.87
XP_022637422.1 31.22 5.49 9.28 54.01
XP_022638520.1 41.80 11.11 10.05 37.04
XP_022638546.1 25.52 5.33 8.76 60.38
XP_022638786.1 25.18 5.32 16.67 52.84
XP_022640184.1 42.74 7.05 6.22 43.98
XP_022640223.1 35.20 3.29 8.55 52.96
XP_022640949.1 40.98 4.89 12.03 42.11
XP_022641765.1 39.80 4.42 8.16 47.62

图2

绿豆与拟南芥R2R3-MYB基因成员的二级结构与三级结构预测"

图3

绿豆R2R3-MYB蛋白序列R2 (A)、R3 (B)结构域"

图4

绿豆R2R3-MYB基因染色体定位 染色体中条带颜色越深, 基因密度越大。The darker the band in the chromosome, the greater the gene density."

图5

绿豆R2R3-MYB基因的区域共线性"

附表3

绿豆R2R3-MYB基因Ka/Ks分析"

序列1
Seq_1		 序列2
Seq_2		 非同义替换率
Ka		 同义替换率
Ks		 非同义替换/同义替换Ka/Ks 有效长度Effective length 平均S位点Average S-sites 平均N位点Average N-sites cN cS pN pS
rna-XM_022780189.1 rna-XM_014635074.1 0.3078 1.0338 0.2977 1224 256.0833 967.9167 244.3333 143.6667 0.2524 0.5610
rna-XM_022780566.1 rna-XM_014635101.2 0.1261 0.8136 0.1550 999 215.6667 783.3333 90.9167 107.0833 0.1161 0.4965
rna-XM_014668607.2 rna-XM_014636033.2 0.1720 1.1622 0.1480 822 180.0000 642.0000 98.6667 106.3333 0.1537 0.5907
rna-XM_014636094.2 rna-XM_014644330.2 0.1882 0.7434 0.2531 750 167.6667 582.3333 96.9167 79.0833 0.1664 0.4717
rna-XM_022778602.1 rna-XM_014636187.2 0.4748 1.7940 0.2647 480 103.5833 376.4167 132.4167 70.5833 0.3518 0.6814
rna-XM_014668447.2 rna-XM_014636254.2 0.2000 1.1215 0.1783 891 195.9167 695.0833 122.0000 114.0000 0.1755 0.5819
rna-XM_014662416.2 rna-XM_014636254.2 0.3013 2.6518 0.1136 936 207.8333 728.1667 180.6667 151.3333 0.2481 0.7281
rna-XM_014639391.2 rna-XM_014636899.2 0.1446 0.7099 0.2037 1191 257.6667 933.3333 122.7500 118.2500 0.1315 0.4589
rna-XM_014638588.2 rna-XM_014638091.2 0.3803 2.2624 0.1681 855 175.8333 679.1667 202.5833 125.4167 0.2983 0.7133
rna-XM_014638588.2 rna-XM_022779510.1 0.1619 0.6790 0.2384 921 192.3333 728.6667 106.0833 85.9167 0.1456 0.4467
rna-XM_014638647.2 rna-XM_014651352.2 0.2537 0.9661 0.2626 729 168.0000 561.0000 120.7500 91.2500 0.2152 0.5432
rna-XM_014638849.2 rna-XM_014654670.2 0.2541 0.5574 0.4558 897 189.4167 707.5833 152.5000 74.5000 0.2155 0.3933
rna-XM_014639712.2 rna-XM_014656820.2 0.1598 0.8404 0.1902 1161 254.2500 906.7500 130.5000 128.5000 0.1439 0.5054
rna-XM_014639857.2 rna-XM_014649871.2 0.1444 0.8683 0.1663 867 185.6667 681.3333 89.5000 95.5000 0.1314 0.5144
rna-XM_014639858.2 rna-XM_014641616.2 0.2792 2.6718 0.1045 870 188.0000 682.0000 159.0000 137.0000 0.2331 0.7287
rna-XM_014639858.2 rna-XM_014652640.2 0.1876 0.9742 0.1926 879 190.0833 688.9167 114.3333 103.6667 0.1660 0.5454
rna-XM_022778513.1 rna-XM_014639953.2 0.3419 1.3375 0.2556 888 186.5833 701.4167 192.5833 116.4167 0.2746 0.6239
rna-XM_014639953.2 rna-XM_014642047.2 0.3041 1.4189 0.2144 930 195.0833 734.9167 183.7500 124.2500 0.2500 0.6369
rna-XM_014640132.2 rna-XM_014657275.2 0.3539 1.4057 0.2518 975 201.0833 773.9167 218.3333 127.6667 0.2821 0.6349
rna-XM_014640928.2 rna-XM_014650862.2 0.4346 2.4693 0.1760 1110 244.4167 865.5833 285.5000 176.5000 0.3298 0.7221
rna-XM_014640928.2 rna-XM_022778211.1 0.3640 2.6801 0.1358 1050 229.6667 820.3333 236.5833 167.4167 0.2884 0.7290
rna-XM_014650612.2 rna-XM_014641616.2 0.1659 0.6854 0.2421 909 202.0000 707.0000 105.2500 90.7500 0.1489 0.4493
rna-XM_014641616.2 rna-XM_014652640.2 0.3519 2.1240 0.1657 861 188.6667 672.3333 188.8333 133.1667 0.2809 0.7058
rna-XM_022778513.1 rna-XM_014642047.2 0.1696 0.6238 0.2719 900 192.8333 707.1667 107.3333 81.6667 0.1518 0.4235
rna-XM_014642071.2 rna-XM_022786044.1 0.1376 0.6361 0.2163 822 180.3333 641.6667 80.6667 77.3333 0.1257 0.4288
rna-XM_014654375.2 rna-XM_014642112.2 0.1436 1.0902 0.1317 597 123.8333 473.1667 61.8333 71.1667 0.1307 0.5747
rna-XM_014663630.2 rna-XM_014642433.1 0.1817 0.7792 0.2331 693 149.0833 543.9167 87.7500 72.2500 0.1613 0.4846
rna-XM_022778602.1 rna-XM_014644084.2 0.5350 3.0374 0.1761 438 97.2500 340.7500 130.3333 71.6667 0.3825 0.7369
rna-XM_014644084.2 rna-XM_014666481.1 0.6052 2.3371 0.2590 294 62.6667 231.3333 96.0833 44.9167 0.4153 0.7168
rna-XM_014644911.2 rna-XM_014648120.2 0.2610 1.8531 0.1408 705 164.3333 540.6667 119.1667 112.8333 0.2204 0.6866
rna-XM_014644911.2 rna-XM_014650374.2 0.3289 1.5390 0.2137 717 170.5833 546.4167 145.5000 111.5000 0.2663 0.6536
rna-XM_014644911.2 rna-XM_014666022.2 0.3525 3.9120 0.0901 753 179.0833 573.9167 161.4167 133.5833 0.2813 0.7459
rna-XM_014645057.2 rna-XM_014651226.2 0.3277 1.8294 0.1791 432 96.1667 335.8333 89.1667 65.8333 0.2655 0.6846
rna-XM_014645094.2 rna-XM_014648737.2 0.2350 1.3692 0.1716 537 132.5833 404.4167 81.5833 83.4167 0.2017 0.6292
rna-XM_014645194.2 rna-XM_014648609.2 0.1841 1.1619 0.1584 966 211.3333 754.6667 123.1667 124.8333 0.1632 0.5907
rna-XM_014645194.2 rna-XM_022784502.1 0.3845 2.3274 0.1652 867 193.0000 674.0000 202.7500 138.2500 0.3008 0.7163
rna-XM_014647830.1 rna-XM_014666838.2 0.3694 1.1299 0.3269 690 149.7500 540.2500 157.5833 87.4167 0.2917 0.5838
rna-XM_014648120.2 rna-XM_014650374.2 0.2990 1.7962 0.1664 798 191.3333 606.6667 149.5833 130.4167 0.2466 0.6816
rna-XM_014649873.2 rna-XM_014663412.2 0.1160 0.8302 0.1397 903 202.6667 700.3333 75.2500 101.7500 0.1074 0.5021
rna-XM_014650374.2 rna-XM_014666022.2 0.1378 1.4860 0.0928 828 199.2500 628.7500 79.1667 128.8333 0.1259 0.6466
rna-XM_014650862.2 rna-XM_022778211.1 0.2296 1.3801 0.1663 1059 227.5833 831.4167 164.4167 143.5833 0.1978 0.6309
rna-XM_014651226.2 rna-XM_022786210.1 0.3563 3.1262 0.1140 582 127.4167 454.5833 128.9167 94.0833 0.2836 0.7384
rna-XM_014651401.2 rna-XM_014664267.2 0.2406 0.7111 0.3383 1257 281.1667 975.8333 200.8333 129.1667 0.2058 0.4594
rna-XM_014668447.2 rna-XM_014652066.2 0.3394 4.3051 0.0788 900 198.7500 701.2500 191.4167 148.5833 0.2730 0.7476
rna-XM_014652066.2 rna-XM_014662416.2 0.2332 0.9009 0.2588 1068 237.5833 830.4167 166.4167 124.5833 0.2004 0.5244
rna-XM_022778990.1 rna-XM_014652475.2 0.4088 2.6224 0.1559 786 177.8333 608.1667 191.6667 129.3333 0.3152 0.7273
rna-XM_014652475.2 rna-XM_014662545.2 0.1670 1.3036 0.1281 888 192.2500 695.7500 104.1667 118.8333 0.1497 0.6181
rna-XM_014652982.2 rna-XM_014664093.2 0.2184 1.2977 0.1683 504 106.4167 397.5833 75.3333 65.6667 0.1895 0.6171
rna-XM_014653907.2 rna-XM_014664607.2 0.0907 0.6921 0.1310 792 167.4167 624.5833 53.3333 75.6667 0.0854 0.4520
rna-XM_014654670.2 rna-XM_014657233.2 0.4056 1.9651 0.2064 846 177.8333 668.1667 209.3333 123.6667 0.3133 0.6954
rna-XM_014655630.2 rna-XM_014655479.2 0.1305 0.8446 0.1545 732 153.2500 578.7500 69.3333 77.6667 0.1198 0.5068
rna-XM_014655630.2 rna-XM_014636037.2 0.3851 1.4787 0.2604 633 131.6667 501.3333 151.0000 85.0000 0.3012 0.6456
rna-XM_022784502.1 rna-XM_014664894.2 0.2244 1.1396 0.1969 912 201.8333 710.1667 137.7500 118.2500 0.1940 0.5859
rna-XM_014667673.2 rna-XM_022775923.1 0.1594 0.6126 0.2601 864 188.9167 675.0833 96.9167 79.0833 0.1436 0.4186
rna-XM_022782825.1 rna-XM_022775636.1 0.1127 0.9731 0.1159 1494 342.0000 1152.0000 120.5833 186.4167 0.1047 0.5451
rna-XM_022785228.1 rna-XM_022780152.1 0.3979 1.9011 0.2093 447 94.2500 352.7500 108.9167 65.0833 0.3088 0.6905

图6

绿豆R2R3-MYB基因保守基序和基因结构"

图7

绿豆R2R3-MYB基因启动子顺式作用元件"

图8

绿豆R2R3-MYB基因不同组织表达热图 VB: 叶柄; VL: 叶片; VP: 下胚轴; VS: 籽粒种皮。"

图9

绿豆R2R3-MYB基因在不同逆境胁迫下的表达分析 *表示处理间在0.05概率水平差异显著。"

图10

绿豆R2R3-MYB基因与类黄酮合成结构基因相关性热图"

图11

绿豆R2R3-MYB与类黄酮合成结构基因的蛋白互作图 线条的颜色和数量表示蛋白之间互作的依据。"

[1] Dubos C, Stracke R, Grotewold E, Weisshaar B, Martin C, Lepiniec L. MYB transcription factors in Arabidopsis. Plant Cell, 2010, 15: 573-581.
[2] Paz-Ares J, Ghosal D, Wienand U, Peterson P A, Saedler H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J, 1987, 6: 3553-3558.
[3] Wang X P, Niu Y L, Zheng Y. Multiple functions of MYB transcription factors in abiotic stress responses. Int J Mol Sci, 2021, 22: 6125.
[4] Jia L, Clegg M T, Jiang T. Evolutionary dynamics of the DNA-binding domains in putative R2R3-MYB genes identified from rice subspecies indica and japonica genomes. Plant Physiol, 2004, 134: 575-585.
[5] Rosinski J A, Atchley W R. Molecular evolution of the Myb family of transcription factors: evidence for polyphyletic origin. J Mol Evol, 1998, 46: 74-83.
[6] Stracke R, Werber M, Weisshaar B. The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol, 2001, 4: 447-456.
[7] Yang J, Zhang S, Li H Q, Wang L T, Liu Y, Niu L L, Yang Q, Meng D, Fu Y J. Genome-wide analysis and characterization of R2R3-MYB family in pigeon pea (Cajanus cajan L.) and their functional identification in phenylpropanoids biosynthesis. Planta, 2021, 254: 64.
[8] 王古悦, 唐慕宁, 牛静雅, 冯雪. 菜豆R2R3-MYB基因家族鉴定及其在BCMV侵染后的表达分析. 山西农业科学, 2023, 51: 1020-1033.
Wang G Y, Tang M N, Niu J Y, Feng X. Identification of R2R3- MYB gene family in common bean (Phaseolus vulgaris L.) and expression analysis after BCMV infection. J Shanxi Agric Sci, 2023, 51: 1020-1033 (in Chinese with English abstract).
[9] Knight H, Trewavas A J, Knight M R. Calcium signalling in Arabidopsis thaliana responding to drought and salinity. Plant J, 1997, 12: 1067-1078.
[10] Van Schie C C, Haring M A, Schuurink R C. Regulation of terpenoid and benzenoid production in flowers. Curr Opin Plant Biol, 2006, 9: 203-208.
[11] Cao Y P, Li K, Li Y L, Zhao X P, Wang L H. MYB transcription factors as regulators of secondary metabolism in plants. Biology, 2020, 9: 61.
[12] Xie X B, Li S, Zhang R F, Zhao J, Chen Y C, Zhao Q, Yao Y X, You C X, Zhang X S, Hao Y J. The bHLH transcription factor MdbHLH3 promotes anthocyanin accumulation and fruit colouration in response to low temperature in apples. Plant Cell Environ, 2012, 35: 1884-1897.
[13] Naing A H, Kim C K. Roles of R2R3-MYB transcription factors in transcriptional regulation of anthocyanin biosynthesis in horticultural plants. Plant Mol Biol, 2018, 98: 1-18.
[14] Li S S, Zhang Y J, Shi L Y, Cao S F, Chen W, Yang Z F. Involvement of a MYB transcription factor in anthocyanin biosynthesis during Chinese bayberry (Morella rubra L.) fruit ripening. Biology, 2023, 12: 894.
[15] Kovinich N, Kayanja G, Chanoca A, Riedl K, Otegui M S, Grotewold E. Not all anthocyanins are born equal: distinct patterns induced by stress in Arabidopsis. Plant Cell, 2014, 240: 931-940.
[16] Chen X S, Wu Y, Yu Z H, Gao Z Y, Ding Q, Shah S H A, Lin W Y, Li Y, Hou X L. BcMYB111 responds to BcCBF2 and induces flavonol biosynthesis to enhance tolerance under cold stress in non-heading Chinese cabbage. Int J Mol Sci, 2023, 24: 8670.
[17] Yu Y, Guo D D, Min D H, Cao T, Ning L, Jiang Q Y, Sun X J, Zhang H, Tang W S, Gao S Q, Zhou Y B, Xu Z S, Chen J, Ma Y Z, Chen M, Zhang X H. Foxtail millet MYB-like transcription factor SiMYB16 confers salt tolerance in transgenic rice by regulating phenylpropane pathway. Plant Physiol Biochem, 2023, 195: 310-321.
[18] Song Q, Kong L F, Yang X R, Jiao B, Hu J, Zhang Z C, Xu C Z, Luo K M. PtoMYB142, a poplar R2R3-MYB transcription factor, contributes to drought tolerance by regulating wax biosynthesis. Tree Physiol, 2022, 42: 2133-2147.
[19] Dahiya P K, Linnemann A R, Van Boekel M A J S, Khetarpaul N, Grewal R B, Nout M J R. Mung bean: technological and nutritional potential. Crit Rev Food Sci Nutr, 2015, 55: 670-688.
[20] Xu H, Zhou Q, Liu B, Cheng K W, Chen F, Wang M F. Neuroprotective potential of mung bean (Vigna radiata L.) polyphenols in alzheimer's disease: a review. J Agric Food Chem, 2021, 69: 11554-11571.
[21] 宋梦媛, 郭中校, 苏禹霏, 邓昆鹏, 兰天娇, 程钰鑫, 包淑英, 王桂芳, 窦金光, 姜泽锴, 王明海, 徐宁. 一种绿豆柱头外露突变体的转录组分析. 作物学报, 2024, 50: 957-968.
Song M Y, Guo Z X, Su Y F, Deng K P, Lan T J, Cheng Y X, Bao S Y, Wang G F, Dou J G, Jiang Z K, Wang M H, Xu N. Transcriptome analysis of a stigma exsertion mutant in mungbean. Acta Agron Sin, 2024, 50: 957-968 (in Chinese with English abstract).
[22] Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol, 2021, 38: 3022-3027.
[23] Bailey T L, Johnson J, Grant C E, Noble W S. The MEME suite. Nucleic Acids Res, 2015, 43: W39-W49.
[24] Chen C J, Chen H, Zhang Y, Thomas H R, Frank M H, He Y H, Xia R. TBtools: an integrative toolkit developed for interactive analyses of big biological data. Mol Plant, 2020, 13: 1194-1202.
[25] Kang C H, Jung W Y, Kang Y H, Kim J Y, Kim D G, Jeong J C, Baek D W, Jin J B, Lee J Y, Kim M O, Chung W S, Mengiste T, Koiwa H, Kwak S S, Bahk J D, Lee S Y, Nam J S, Yun D J, Cho M J. AtBAG6, a novel calmodulin-binding protein, induces programmed cell death in yeast and plants. Cell Death Differ, 2006, 13: 84-95.
[26] Yang J H, Zhang B H, Gu G, Yuan J Z, Shen S J, Jin L, Lin Z Q, Lin J F, Xie X F. Genome-wide identification and expression analysis of the R2R3-MYB gene family in tobacco (Nicotiana tabacum L.). BMC Genomics, 2022, 23: 432.
[27] 蒋宇佳, 于元平, 孙向一, 周敏, 吴春妍, 李玉华, 刘明稀. 假俭草R2R3-MYB基因家族的鉴定及其在干旱胁迫下的表达模式分析. 草地学报, 2023, 31: 2628-2641.
Jiang Y J, Yu Y P, Sun X Y, Zhou M, Wu C Y, Li Y H, Liu M X. Identification of R2R3-MYB gene family and analysis of its expression pattern in centipedegrass under drought stress. Acta Agrest Sin, 2023, 31: 2628-2641 (in Chinese with English abstract).
[28] Ren L L, Liu Y J, Liu H J, Qian T T, Qi L W, Wang X R, Zeng Q Y. Subcellular relocalization and positive selection play key roles in the retention of duplicate genes of populus class III peroxidase family. Plant Cell, 2014, 26: 2404-2419.
[29] 居利香, 雷欣, 赵成志, 舒黄英, 汪志伟, 成善汉. 辣椒MYB基因家族的鉴定及与辣味关系分析. 园艺学报, 2020, 47: 875-892.
Ju L X, Lei X, Zhao C Z, Shu H Y, Wang Z W, Cheng S H. Identification of MYB family genes and its relationship with pungency of pepper. Acta Hortic Sin, 2020, 47: 875-892 (in Chinese with English abstract).
[30] Gonzalez A, Zhao M Z, Leavitt J M, Lloyd A M. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J, 2008, 53: 814-827.
[31] Millar A A, Gubler F. The Arabidopsis GAMYB-like genes, MYB33 and MYB65, are microRNA-regulated genes that redundantly facilitate anther development. Plant Cell, 2005, 17: 705-721.
[32] Reyes J L, Chua N H. ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination. Plant J, 2007, 49: 592-606.
[33] Agarwal M, Hao Y J, Kapoor A, Dong C H, Fujii H, Zheng X W, Zhu J K. A R2R3 type MYB transcription factor is involved in the cold regulation of CBF genes and in acquired freezing tolerance. J Biol Chem, 2006, 281: 37636-37645.
[34] Wang Y J, Zhang Y, Fan C J, Wei Y C, Meng J X, Li Z, Zhong C L. Genome-wide analysis of MYB transcription factors and their responses to salt stress in Casuarina equisetifolia. Plant Cell, 2021, 21: 328.
[35] 琚吉浩, 马超, 王添宁, 吴毅, 董钟, 方美娥, 陈钰姝, 张均, 付国占. 小麦TaPOD家族的全基因组鉴定及表达分析. 作物学报, 2024, 50: 779-821.
Ju J H, Ma C, Wang T N, Wu Y, Dong Z, Fang M E, Chen Y S, Zhang J, Fu G Z. Genome wide identification and expression analysis of TaPOD family in wheat. Acta Agron Sin, 2024, 50: 779-821 (in Chinese with English abstract).
[36] Byrne M E, Barley R, Curtis M, Arroyo J M, Dunham M, Hudson A, Martienssen R A. Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis. Plant Cell, 2000, 408: 967-971.
[37] Cheng H, Song S S, Xiao L T, Soo H M, Cheng Z W, Xie D X, Peng J R. Gibberellin acts through jasmonate to control the expression of MYB21, MYB24, and MYB57 to promote stamen filament growth in Arabidopsis. PLoS Genet, 2009, 5: e1000440.
[38] Li J R, Chen X Z, Zhou X X, Huang H L, Wu D D, Shao J Q, Zhan R T, Chen L K. Identification of trihelix transcription factors in Pogostemon cablin reveals PatGT-1 negatively regulates patchoulol biosynthesis. Ind Crops Prod, 2021, 161: 113182.
[39] Lou X, Yao S, Chen P Z, Wang D B, Agassin R H, Hou Y Q, Zhang C, Ji K S. Transcriptome identification of R2R3-MYB gene family members in Pinus massoniana and PmMYB4 response to drought stress. Forests, 2023, 14: 410.
[40] Shin R, Burch A Y, Huppert K A, Tiwari S B, Murphy A S, Guilfoyle T J, Schachtman D P. The Arabidopsis transcription factor MYB77 modulates auxin signal transduction. Plant Cell, 2007, 19: 2440-2453.
[41] Jung C, Seo J S, Han S W, Koo Y J, Kim C H, Song S I, Nahm B H, Choi Y D, Cheong J J. Overexpression of AtMYB44 enhances stomatal closure to confer abiotic stress tolerance in transgenic Arabidopsis. Plant Physiol, 2008, 146: 623-635.
[42] Fang S Y, Qiu S, Chen K X, Lyu Z Y, Chen W S. The transcription factors SbMYB45 and SbMYB86.1 regulate flavone biosynthesis in Scutellaria baicalensis. Plant Physiol Biochem, 2023, 200: 107794.
[43] Xu H S, Dai X, Hu X, Yu H Z, Wang Y, Zheng B S, Xu J, Wu X Q. Phylogenetic analysis of R2R3-MYB family genes in Tetrastigma hemsleyanum Diels et gilg and roles of ThMYB4 and ThMYB7 in flavonoid biosynthesis. Biomolecules, 2023, 13: 531.
[44] Ma C, Feng Y L, Zhou S, Zhang J, Guo B B, Xiong Y, Wu S W, Li Y, Li Y J, Li C X. Metabolomics and transcriptomics provide insights into the molecular mechanisms of anthocyanin accumulation in the seed coat of differently colored mung bean (Vigna radiata L.). Plant Physiol Biochem, 2023, 200: 107739.
[45] 张静. 气象因素对绿豆种植的影响. 科学大众, 2008, (11): 128.
Zhang J. The influence of meteorological factors on mung bean cultivation. Popular Sci, 2008, (11): 128 (in Chinese with English abstract).
[46] 陈旭微, 杨玲, 章艺. 低温下的绿豆下胚轴细胞超微结构变化. 植物生理学通讯, 2006, 42: 1099-1102.
Chen X W, Yang L, Zhang Y. Changes in ultrastructure of hypocotyl cells in Phaseolus radiatus Linn. under low temperature condition. Plant Physiol Commun, 2006, 42: 1099-1102 (in Chinese with English abstract).
[47] Cominelli E, Galbiati M, Vavasseur A, Conti L, Sala T A, Vuylsteke M, Leonhardt N, Dellaporta S L, Tonelli C. A guard-cell- specific MYB transcription factor regulates stomatal movements and plant drought tolerance. Curr Biol, 2005, 15: 1196-1200.
[48] Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K. Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell, 2003, 15: 63-78.
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