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作物学报 ›› 2026, Vol. 52 ›› Issue (11): 3293-3307.doi: 10.3724/SP.J.1006.2026.64058

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

高粱SbCPP01基因过表达提高拟南芥的耐盐碱性

杜品廷1，周伟1，王振国2，李岩2，刘新宇1，张诗琪1，王金月1，包雪莲2，周亚星1,*   

  1. 1 内蒙古民族大学农学院, 内蒙古通辽 028000; 2 通辽市农牧业科学院, 内蒙古通辽 028000
  • 收稿日期:2026-04-30 修回日期:2026-08-21 接受日期:2026-08-21 出版日期:2026-11-12 网络出版日期:2026-08-26
  • 通讯作者: 周亚星, E-mail: 18747341055@163.com
  • 基金资助:
    研究由内蒙古自然科学基金项目(2025MS03084)，内蒙古科技计划项目(2025YFDZ0052)，内蒙古自治区高等学校青年科技英才项目(NJYT22052)和通辽市科技计划项目(TL2025YF004)资助。

SbCPP01 overexpression improves saline-alkali tolerance in Arabidopsis

Du Pin-Ting1,Zhou Wei1,Wang Zhen-Guo2,Li Yan2,Liu Xin-Yu1,Zhang Shi-Qi1,Wang Jin-Yue1,Bao Xue-Lian2,Zhou Ya-Xing1,*   

  1. 1 College of Agriculture, Inner Mongolia Minzu University, Tongliao 028000, Inner Mongolia, China; 2 Tongliao Academy of Agricultural and Animal Husbandry Sciences, Tongliao 028000, Inner Mongolia, China
  • Received:2026-04-30 Revised:2026-08-21 Accepted:2026-08-21 Published:2026-11-12 Published online:2026-08-26
  • Supported by:
    This study was supported by the Inner Mongolia Natural Science Foundation (2025MS03084), the Inner Mongolia Science and Technology Plan Project (2025YFDZ0052), the Young Talents in Science and Technology Program of Institutions of Higher Education of Inner Mongolia Autonomous Region (NJYT22052), and the Tongliao Science and Technology Plan Project (TL2025YF004).

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摘要：

富含半胱氨酸的多梳样蛋白(CPP)转录因子在植物应对逆境胁迫中发挥关键的调控作用。基于前期对高粱CPP基因家族的生物信息学分析以及结合转录组数据以及RT-qPCR，筛选出的SbCPP01基因可能在盐碱胁迫下存在功能，因此为验证高粱CPP基因在盐碱胁迫下的功能，本研究对SbCPP01基因进行克隆和功能研究。利用反转录PCR克隆获得SbCPP01的编码序列并构建过表达载体，将其过表达至拟南芥中来研究其功能。在相同的盐碱胁迫条件下，SbCPP01显著增强了转基因拟南芥的耐盐碱性，表现为盐碱胁迫条件下植株发芽率、根长及鲜重均显著高于野生型。且转基因株系的POD活性、SOD活性、CAT活性、可溶性蛋白含量、可溶性糖含量、淀粉含量和脯氨酸含量均优于野生型，H2O2含量、O2?含量和MDA含量均低于野生型，说明SbCPP01能够提高抗氧化酶的活性，减少活性氧的累积。对拟南芥幼苗胁迫7 d后，通过对其表型进行观察发现，野生型拟南芥的叶片枯萎发黄，转基因拟南芥生长情况良好。在盐碱胁迫处理下，通过RT-qPCR分析盐碱胁迫关键基因的表达量，发现转基因株系中AtSOS1、AtSOS3、AtP5CS1、AtCBL10、AtLEA3、AtNHX1、AtRD29A和AtNCED3基因的表达量均高于野生型。因此，SbCPP01过表达可以提高拟南芥的耐盐碱性。SbCPP01可作为盐碱抗性分子育种的目标基因，为高粱及其他作物的耐逆遗传改良提供理论支撑和技术手段。

关键词: 高粱, SbCPP01基因, 盐碱胁迫, 拟南芥, 转基因

Abstract: The cysteine-rich polycomb-like protein (CPP) transcription factor plays a pivotal regulatory role in plant responses to stress conditions. Based on previous bioinformatics analysis of the CPP gene family in sorghum, combined with transcriptome data and RT-qPCR results, SbCPP01 was identified as a gene potentially involved in the response to saline-alkali stress. To verify the role of SbCPP01 in saline-alkali tolerance, we performed cloning and functional characterization of SbCPP01. The coding sequence of SbCPP01 was amplified via reverse transcription PCR, and an overexpression vector was constructed. The vector was subsequently introduced into Arabidopsis thaliana for functional assays. Under identical saline-alkali stress conditions, overexpression of SbCPP01 significantly improved the saline-alkali tolerance of transgenic Arabidopsis. Compared with wild-type plants, the transgenic lines showed higher germination rates, longer root lengths and greater fresh weight. Transgenic plants also exhibited higher activities of POD, SOD and CAT, along with increased contents of soluble protein, soluble sugar, starch and proline. By contrast, their levels of hydrogen peroxide (H2O2), superoxide anion (O2?) and malondialdehyde (MDA) were lower than those of the wild type. These findings indicate that SbCPP01 enhances antioxidant enzyme activities and alleviates reactive oxygen species accumulation. After 7 days of stress treatment, phenotypic observations revealed that wild-type seedlings suffered leaf yellowing and wilting, while transgenic lines grew well. RT-qPCR analysis further demonstrated that the expression levels of stress-responsive genes, including AtSOS1, AtSOS3, AtP5CS1, AtCBL10, AtLEA3, AtNHX1, AtRD29A and AtNCED3, were markedly upregulated in transgenic plants under saline-alkali stress. Collectively, overexpression of SbCPP01 confers enhanced saline-alkali tolerance in Arabidopsis thaliana. As a promising candidate gene for molecular breeding for saline-alkali resistance, SbCPP01 provides a theoretical basis and technical support for the genetic improvement of stress tolerance in sorghum and other crops.

Key words: sorghum, SbCPP01 gene, saline-alkali stress, Arabidopsis thaliana, genetic transformation

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