关键词: 硫氧还蛋白h, 发芽, 大麦

Abstract: Thioredoxin h (Trx h) is a small disulfide-containing redox protein. During seed germination Trx h amylase，protease and other enzyme activities can be enhanced and the hydrolysis of the endosperm storage substances such as starch and protein promoted by Trx h. It’s very likely to affect the malting barley qualith by changing Trx h activity. Thioredoxin s (trxs) gene, isolated from Phalaris coerulescens, belongs to thioredoxin(trx)gene family. As far as catalysis reduction reaction of S-S is concemed, expression product of trxs gene has the same bio-function as Trx h. Trx h activity may be enhanced and carbon-nitrogen metabolic activity during seed genmination promoted and diastatic power(DP) and extract material of malting barley increased by transferring trxs into barley. To achieve barley with higher Trx h activity, trxs gene had been transformed into barley immature embryos and transgenic plants had been obtained via tissue culture in previous study. In this study, ... ...

Key words: Thioredoxin h, Germination, Barley