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作物学报 ›› 2009, Vol. 35 ›› Issue (10): 1851-1857.doi: 10.3724/SP.J.1006.2009.01851

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

以分子标记辅助选择育成抗条纹叶枯病水稻新品种“武陵粳1号”

潘学彪1*,陈宗祥1,左示敏1,张亚芳1,吴旭江1,马宁1,江祺祥2,阙金华3,周春和3   

  1. 1扬州大学作物遗传生理江苏省重点实验室/植物功能基因组学教育部重点实验室,江苏扬州225009;2江苏省常州市武进区稻麦育种场,江苏武进213149;3江苏省种子管理站,江苏南京210036
  • 收稿日期:2009-02-02 修回日期:2009-04-27 出版日期:2009-10-12 网络出版日期:2009-08-07
  • 通讯作者: 潘学彪, E-mail: shuidao@yzu.edu.cn
  • 基金资助:

    本研究由国际科技支撑计划项目(2006BAD01A01-5)和引进国际先进农业科学技术计划(948计划)重大项目(2006-G51)资助。

A New Rice Cultivar“Wulingjing1” with Resistance to Rice Stripe Virus Bred by Marker Assisted Selection

PAN Xue-Biao1,*,CHEN Zong-Xiang1,ZUO Shi-Min1,ZHANG Ya-Fang1,WU Xu-Jiang1,MA Ning1,JIANG  Qi-Xiang2,QUE Jin-Hua3,ZHOU  Chun-He3   

  1. 1 Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics of Education Ministry, Yangzhou University, Yangzhou 225009, China; 2 Wujin Rice and Wheat Breeding Station, Changzhou 213149, China; 3 Seeds Management Station of Jiangsu Province, Nanjing 210036, China
  • Received:2009-02-02 Revised:2009-04-27 Published:2009-10-12 Published online:2009-08-07
  • Contact: PAN Xue-Biao, E-mail: shuidao@yzu.edu.cn

摘要:

条纹叶枯病是21世纪以来江苏省最重要的水稻病害之一。食味品质较优的主栽品种武育粳3号因高度感染该病而种植面积锐减。本研究以镇稻88为抗条纹叶枯病毒病基因Stvb-i的供体亲本,采用回交育种策略,改良武育粳3号的抗条纹叶枯病性能。在连续回交和自交过程中,以紧密连锁的双侧分子标记对Stvb-i进行前景选择,同时对后代与轮回亲本遗传背景的相似程度进行分子标记辅助背景选择,在短期内育成抗条纹叶枯病的武育粳3,命名为武陵粳1。新品种保持了原品种的基本农艺性状、丰产性、稳产性和优异的食味品质,并大幅度提高了其条纹叶枯病抗性水平,在江苏省多点抗性鉴定试验中的平均病株率仅为4.4%,极显著低于原品种(53.2%)

关键词: 水稻条纹叶枯病, 抗性基因供体, 回交育种, 轮回亲本, 标记辅助选择

Abstract:

Rice stripe virus disease is one of the most serious diseases in rice production area of Jiangsu province since 2002. A commercial rice cultivar with excellent food taste-quality, Wuyujing 3, was highly susceptible to the disease and was improved through the MAS strategy in a backcross project with “Zhendao 88” as donor parent of resistance gene Stvb-i. In each backcross generation, we selected the progenies with Stvb-i based on the closely flanked markers. In advanced backcross generations, the marker’s detection for genetic background of selected plants was performed, along with the estimate by the eye for the similarity of selected plants to recurrent parent in synthetic agronomic traits. As a result, a new cultivar, Wulingjing 1, was fleetingly bred in BC3F4 generation, whose resistance to the disease was significantly increased and main characters especially on excellent taste-quality were consistent with those of the recurrent parent Wuyujing 3. In resistance identification tests with multiple locations organized by Seed Management Station of Jiangsu Province, the average percent of affected plants of the new cultivar was only 4.4% and significantly lower than that of recurrent parent (53.2%).

Key words: Rice stripe disease, Resistance gene donor, Backcross breeding, Current parent, MAS

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