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作物学报 ›› 2009, Vol. 35 ›› Issue (12): 2213-2217.doi: 10.3724/SP.J.1006.2009.02213

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻黑条矮缩病抗性QTL分析

潘存红1,2，李爱宏2,**，陈宗祥1，吴林波1，戴正元2，张洪熙2，黄年生2，陈夕军1，张亚芳1，左示敏1，潘学彪1,*   

  1. 1扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室，江苏扬州225009；2江苏里下河地区农业科学研究所/国家水稻改良中心南京分中心，江苏扬州225007
  • 收稿日期:2009-03-11 修回日期:2009-06-25 出版日期:2009-12-10 网络出版日期:2009-09-10
  • 通讯作者: 潘学彪, E-mail: shuidao@yzu.edu.cn; Tel: 0514-87972136
  • 基金资助:

    本研究由江苏省自然科学基金项目（BK2008202）和江苏里下河地区农业科学研究所基金项目（200801）资助。

Detection of QTL for Resistance to Rice Black-Streaked Dwarf  Disease

PAN Cun-Hong1,2,LI Ai-Hong2,**,CHEN Zong-Xiang1,WU Lin-Bo1,DAI Zheng-Yuan2, HUANG Nian-Sheng2,CHEN Xi-Jun1,ZHANG Ya-Fang1,ZUO Shi-Min1,PAN Xue-Biao1*   

  1. 1Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province,Yangzhou University,Yangzhou 225009,China；2Lixiahe Agricultural Research Institute of Jiangsu Province/Nanjing Subcenter of National Rice Improvement Center，Yangzhou 225007，China
  • Received:2009-03-11 Revised:2009-06-25 Published:2009-12-10 Published online:2009-09-10
  • Contact: PAN Xue-Biao, E-mail: shuidao@yzu.edu.cn; Tel: 0514-87972136

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1846

被引次数

32

摘要：

利用珍汕97B/明恢63的重组自交系群体，采用自然发病鉴定的方法，以穴发病率为表型值，对各株系进行黑条矮缩病抗性鉴定。群体的穴发病率偏向于抗病亲本，且呈连续性分布，表明水稻黑条矮缩病抗性受数量性状基因控制。利用WinQTLcart 2.5软件对黑条矮缩病抗性QTL进行分析，共检测到6个QTL，其中第6、11染色体上各有2个，第7、9染色体各有1个。第6、7、9染色体上的4个QTL在两个地点都能检测到，是稳定表达的QTL，尤其是第6染色体上的2个QTL，LOD值分别为12.09和9.77，贡献率分别为20.20%和18.68%，是主效QTL，能在分子标记辅助选择育种中加以利用。

关键词: 水稻黑条矮缩病, 重组自交系群体, QTL分析

Abstract:

The rice black-streaked dwarf viral disease (RBSDV), transmitted by the planthopper Laodelphax striatellus Fallèn, was one of the most serious diseases in rice in south China. Breeding of RBSDV resistance variety using marker-assisted selection was effective measure to reduce the disease damage. For achieving this aim, QTL mapping of resistance to RBSDV was studied using a recombinant inbred line (RIL) population from the cross between Zhenshan 97B and Minghui 63. Reactions of 242 RILs to RBSDV were investigated by natural infection method in Agricultural College of Yangzhou University and Lixiahe Agricultural Research Institute, and scored by the incidence of RBSDV. The trait of RBSDV incidence was normally distributed and marked bias towards the resistant parent, which implied that they were controlled by quantitative trait loci. Six QTLs for resistance to RBSDV were detected by WinQTLcart 2.5 software. Two adjacent QTLs were mapped on chromosome 6, two on chromosome 11, one on chromosomes 7 and 9, respectively. Four QTLs on chromosomes 6, 7, and 9 could be detected in two locations, and were stably expressed across the two environments. There had not been any reports about location of QTL for resistance of rice stripe virus and planthopper, which suggested that six QTLs in this study were really resistant to RBSDV. Two QTLs on chromosome 6 were with main effect, and their contributions to the total variation were 20.20% and 18.68% with LOD scores of 12.09 and 9.77 respectively, moreover, they had high value for study on genetics of RBSDV and should be useful in marker-assisted selection for resistance to RBSDV.

Key words: Rice black-streaked dwarf viral disease, Recombinant inbred line population, QTL Analysis

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